Amersham Pharmacia Biotech AKTA P-920 User Manual
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Amersham Pharmacia Biotech AKTA P-920 User Manual

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Pump P-920
User Manual
18-1125-54

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Summary of Contents for Amersham Pharmacia Biotech AKTA P-920

  • Page 1 Pump P-920 User Manual 18-1125-54...
  • Page 2 Important user information Trademarks ÄKTA, FPLC and UNICORN are trademarks of Amersham Pharmacia Biotech Limited or its subsidiaries. Meaning: Consult the instruction manual to avoid personal injury or damage to the product or other Amersham is a trademark of Nycomed Amersham plc.

  • Page 3: Table Of Contents

    Contents Contents Introduction General ........................1 Accessories......................2 Safety ........................3 Installation Unpacking......................4 General precautions ....................4 Connecting electrical signal cables .................4 Connecting to UniNet-1 communication link ............5 Connecting to supply voltage.................5 Rinsing tubing .......................6 Connecting the inlet and outlet tubing..............6 Running-in the new pump ..................7 Installation of accessories..................8 Operation On/off ........................9...
  • Page 4 Contents Trouble-shooting General ........................23 Faults and actions ....................23 Error messages .....................25 Checking the pump pressure ................27 Reference information Description ......................28 Module .....................28 Rear panel....................28 Fluid delivery ....................29 6-port pump valve..................29 Pressure monitoring ..................30 Protective covers ..................30 Using an external chart recorder ...............30 Menus........................31 Check menu ....................31 B.1.1...
  • Page 5 Contents About this manual This manual comprises two parts; a practical part (sections 1 – 5) and a reference part (sections A – D). Sections 1 – 5 contain the necessary information for installing, operating and maintaining the instrument. Pump P-920 User Manual 18-1125-54 Edition AB...
  • Page 6 Contents Pump P-920 User Manual 18-1125-54 Edition AB...

  • Page 7: Introduction

    3.0 or higher. ™ Pump P-920 works with a wide range of columns and media supplied by Amersham Pharmacia Biotech. The pump can be used to form accurate and reproducible gradients. In stand-alone configurations, setup parameters are stated in a Setup menu, see Reference information B.2.

  • Page 8: Accessories

    Introduction 1.2 Accessories Mixer M-925 can be connected to the pump. When P-920 is connected to UNICORN, the mixer can be controlled on/off/auto from UNICORN. When P-920 is run as a stand-alone unit, the mixer is operated via the local user interface. Pump P-920 User Manual 18-1125-54 Edition AB...

  • Page 9: Safety

    Introduction 1.3 Safety • The module is designed for indoor use only. • Do not use in a dusty atmosphere or close to spraying water. • Do not block the air inlet and outlet of the unit. WARNING! The module must be connected to a grounded mains socket.

  • Page 10: Installation

    Installation 2 Installation 2.1 Unpacking Unpack the module and check the items against the supplied packing list. Inspect the items for obvious damage that may have occurred during transportation. We recommend that all packing materials be retained if onward transport of the module is expected. CAUTION! Read the following information carefully to ensure that the module is installed correctly.

  • Page 11: Connecting To Uninet-1 Communication Link

    Installation Connecting to chart recorder (if used) 1 Connect the chart recorder to the Mini-DIN-socket Pressure using the cable supplied. Analogue out 0-1 V Signal Pressure signal Signal ground 3–6 Not used (reserved for factory testing) Note: The signal cable is delivered with protective covers on each wire.

  • Page 12: Rinsing Tubing

    Installation 2.6 Rinsing tubing The rinsing tubing for the piston seals is installed at the factory and filled with 20% ethanol. Check that the rinsing solution is present behind the pistons and that the rinsing tubing is connected and undamaged. Always use 20% ethanol as rinsing solution. Change rinsing solution at least every month.

  • Page 13: Running-In The New Pump

    Installation 2.8 Running-in the new pump General To be able to follow this section, you must know how to operate the pump. Read through section 3 Operation before continuing. Note: Running with air is not harmfull to the pump sealings. However, the sealing ring lifetime might be shortened if the pump pumps air for long periods.

  • Page 14: Installation Of Accessories

    Installation Installation of accessories Install Mixer M-925 as follows: -Mount the attached Use one of the mounting bracket with mounting rails the two screws -Loosen the two screws CAUTION! Before connecting the mixer M-925, make sure the power switch is in OFF position. Pressure Analogue out 0-1 V The mixer is...

  • Page 15: Operation

    Operation 3 Operation 3.1 On/off Switch on the module at the mains switch on the front panel. At Selftest switch on, the module performs a selftest. Several beeps are heard Please wait... during this process. If an error is detected, an error message is shown.

  • Page 16: Menu Overview

    Operation Selecting a value A cursor below a text or numerical value shows what is affected by the dial. To increase the value, turn the dial clockwise. To decrease the value, turn the dial counter-clockwise. The value can be reset by turning the dial several clicks counter-clockwise.

  • Page 17: Starting And Stopping The Pump

    Operation 3.4 Starting and stopping the pump WARNING! Incorrectly fitted tubing may loosen, causing a jet of liquid to spray out. This is especially dangerous if hazardous chemicals are being used. Connect the tubing by first inserting the tubing fully, then tightening the connector fingertight. WARNING! All protective covers over internal capillaries, piston rods and glass cylinders must be in place when running the pump.

  • Page 18: Setting The Flow Rate And Starting The Pump

    Operation Preparation before starting CAUTION! Before the start of each run, always ensure that there is an adequate supply of eluent in the reservoirs. Do not allow the pump to run dry, since this will affect the lifetime of the sealings. 1 Check that there is sufficient solvent present for the run, and that the solvent filter is fully immersed.

  • Page 19: Running A Simple Gradient

    Operation 3.7 Running a simple gradient Gradients can be run in time or volume base. The default is time base. To change base, see B.2.4 in section Reference information. The gradient is run from current 100% concentration to target concentration, Gradient length Target over a set time or volume.

  • Page 20: Changing Eluent

    Operation Pump wash operation 1 Immerse the inlet filters in a wash solution. Select operating menu Pump Wash 20 ml/min Pump Wash and press 2 When the display shows , select Pump Wash A B&A B&A Pump Wash 20 ml/min and press B&A First pump B and then pump A is washed in sequence.

  • Page 21: Restart After Power Failure

    Operation 3.10 Restart after power failure If the power supply to the pump is interrupted, it automatically restarts after performing a selftest when power is restored. All values under the menu are retained. Other operating values, e.g. flow Setup rate, are reset. Pump P-920 User Manual 18-1125-54 Edition AB...

  • Page 22: Maintenance

    Maintenance 4 Maintenance Note: The piston seals have a limited lifetime depending on the flow rate, pressure and eluents used. The seals are regarded as consumables and are available as a sealing kit. The wear of the piston seals is not covered by the warranty of the pump. A typical lifetime is around 1000 run hours.

  • Page 23: General Care

    Maintenance 4.3 General care Inspect the pump daily for eluent leaks. If, at any time, air bubbles are trapped in a cylinder assembly, Change direction purge the pump by manually changing the direction of the piston A&B when the piston reaches its end position. Change direction several times until the air bubbles are removed.

  • Page 24: Testing Leakage

    Maintenance 4.5 Testing leakage When using the Pump P-920 regularly, a leakage test should be performed every second month. When the pump is used intermittently, a leakage test should be performed before every use. After disassembly due to cleaning or repair, a leakage test should also be performed before use.

  • Page 25: Replacing The Piston Seal

    Maintenance 4.6 Replacing the piston seal If there are signs of leakage between piston seal and glass cylinder, replace the piston seal of the leaking pump cylinder. A seal leakage will gradually fill up the rinsing chamber completely, causing excess fluid to leak out around the rinsing tubing connections. CAUTION! Do not disassemble the pump mechanism unless there is good reason to believe that the seal is leaking.
  • Page 26 Maintenance 3 Switch OFF the pump at the mains power switch on the front Rinsing tubing panel. 4 Remove the protective perspex cover by unscrewing the fixing screws. 5 Remove the protective metal cover over the piston rod by gently Covers Philips screws pressing it together while pulling it off.
  • Page 27 Maintenance 17 Make sure that both the end piece gasket and the wiper are in position. Insert the two support rods into the holes in the end piece. When the glass cylinder is properly seated, tighten the two M3 nuts gently. Inlet connector Pump cylinder assembly Piston rod...

  • Page 28: Replacing A Damaged Piston

    Maintenance 4.7 Replacing a damaged piston Typical symptoms of a damaged piston are observed as excessive piston seal wear, unstable pressure, a reduction in the flow or, in some cases, noise as the piston moves. The piston should be removed, examined for damage or salt precipitation and then replaced with a new piston if necessary.

  • Page 29: Trouble-Shooting

    2 Clean and dry the system/module with a dry cloth or paper. If necessary, tilt the system/module backwards to drain. 3 Call Amersham Pharmacia Biotech local service for advice. No text on the front display 1 Check that the mains cable is connected and the power switch is in ON-position I.
  • Page 30 Trouble-shooting Fault Action Blockage or partial blockage 1 Flush through to clear blockage. 2 If necessary, replace tubing. 3 Check inlet tubing filter. It can become clogged if unfiltered buffers or samples are applied. See instructions for flushing through at the end of the run in section 3.8 Ending the run and storage .

  • Page 31: Error Messages

    Trouble-shooting 5.3 Error messages If the suggested actions do not correct the fault, call Amersham Pharmacia Biotech. Message Action 1 Reboot system. 1 ERROR in software 2 If error remains, notify instrument support. notify instr support 33 ERROR in software notify instr support 34 ERROR in software notify instr support...
  • Page 32 Trouble-shooting 1 Reboot system. 2 If error remains, call service. Failed to set offset 1 Call service for recalibration. 86 ERROR in EEPROM Recalib. all values 1 Select other instruction, or change mode (End, Run or 88 Not allowed in Pause).

  • Page 33: Checking The Pump Pressure

    Trouble-shooting 1 The key was pressed during self-test, or is faulty. ERROR key (OK) 2 Switch off the module. 3 Switch on the module. ERROR key (Esc) ERROR key (OK+Esc) 1 Switch off the module. ERROR number 100 2 Check all connections. 3 Switch on the module.

  • Page 34: A-D Reference Information

    Reference information Reference information Description A.1 Module Pump P-920 is a high performance laboratory pump for use in liquid chromatography and other applications where constant liquid flow is required. An eluent in an external vessel is drawn into the 6-port pump valve by the action of the pump cylinder assemblies.

  • Page 35: Fluid Delivery

    Reference information Auxiliary equipment with digital signal interface can be connected to the 9-pole D-SUB female REMOTE connector (5 V TTL signals only). Signal Function Digital input 1-4 Active status=low or closed terminal to pin 5 Signal ground Digital ouput 1-4 Active status=+5 V in reference to pin 5 When used in combination with UNICORN and the ÄKTA FPLC...

  • Page 36: Pressure Monitoring

    Reference information In stand-alone applications, all connections except fitted at the factory. When used in ÄKTA chromatography FPLC systems, the connections are also connected at the factory. A.5 Pressure monitoring The pressure generated by the pump is continuously monitored by a pressure transducer housed in an outlet manifold block.

  • Page 37: Menus

    Reference information Menus B.1 Check menu Select main operating menu and press to access the check Check sub menus. B.1.1 Checking piston status 1 Select operating menu and press Check Piston Status Check Piston Status 2 The display shows if Left and Right cylinder assemblies are compressing for both pump modules A and B.

  • Page 38: Setup Menu

    Reference information B.2 Setup menu Select main operating menu and press to access the setup Setup sub menus. B.2.1 Set pressure limit Sets high and low pressure limits. When the limit is reached, the pump is set in mode, a buzzer sounds and an error message Pause (for ascending pressure) or (for descending...

  • Page 39: Set Pulse Compensation Mode

    Reference information B.2.6 Set pulse compensation mode Sets the condition for pulse compensation. 1 Select sub menu , press Setup Pulsecomp. Setup Pulsecomp. (on) on off 2 Select either , press B.2.7 Set pressure offset Allows pressure offset calibration. 1 Select sub menu , press Setup Press Offset Setup Press Offset...

  • Page 40: Alarm Timer

    Reference information B.3 Alarm timer You can set the alarm function to either a fixed alarm time or use a count-down timer. The pump can be started or stopped automatically, or an alarm can sound, at the set time. It is not possible to set both an alarm time and the count-down timer.

  • Page 41: Menu And Text Overview

    Reference information B.5 Menu and text overview Turn to select menus on same level. Press to acknowledge Dial selection. Press to move up one menu level. Press again to move up further. 1.00ml/min 1.00ml/min Pause 1.00ml/min Hold 1.00ml/min End Hold Pause End Hold Continue End Continue Pause Set Concentration B...

  • Page 42: C Technical Specifications

    Reference information Technical specifications C.1 Operating data Flow rate range isocratic mode 0.01–20 ml/min in steps of 10 µl/min gradient mode 0.1–20 ml/min in steps of 1 µl/min Pressure range 0–5 MPa (50 bar, 725 psi) Pressure pulsation Max. 6% (dP/P) during pump stroke pH stability range 1–13, (1–14 <1 day exposure) Viscosity...
  • Page 43 Biotech except for alterations described in the User Manual • used as a “stand-alone” unit or connected to other CE labelled Amersham Pharmacia Biotech modules or other products as recommended. Safety standards This product meets the requirement of the Low Voltage Directive (LVD) 73/23/EEC through the harmonised standard EN 61010-1.

  • Page 44: D Accessories And Consumables

    Reference information Accessories and Consumables Item Quant./pack A/C* Code no. Sealing kit, containing two sealings two gaskets and two wipers 18-1032-16 Pump head complete 18-1032-15 Glass cylinder 18-1512-01 Internal tubing kit P-920 18-1128-70 Rinsing tubing, 1.1 mm i.d., 3.1 mm o.d. 18-1032-11 Union 1/16”...

  • Page 48: Short Instructions On

    Short instructions The following short instructions are intended as a guide to users who are fully familiar with the safety precautions and operating instructions described in this manual. These short instructions assume that the module is installed according to the installation instructions. Select with the mains switch Switch on the module...

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