Thermo 3307 Operating And Maintenance Manual

Steri-cult co2 incubator controlled rh with sterilization cycle
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Models:
3307/3308 and 3310/3311
Steri-Cult CO
Incubator
2
Controlled RH with Sterilization Cycle
Operating and Maintenance Manual
Manual No: 7013307 Rev. 3

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Summary of Contents for Thermo 3307

  • Page 1 Models: 3307/3308 and 3310/3311 Steri-Cult CO Incubator Controlled RH with Sterilization Cycle Operating and Maintenance Manual Manual No: 7013307 Rev. 3...
  • Page 2 Thermo Electron Corporation makes no representa- tions or warranties with respect to this manual. In no event shall Thermo be held liable for any damages, direct or incidental, aris- ing out of or related to the use of this manual.
  • Page 3 Steri-Cult Incubator _________________________________________________________________________Warnings Important operating and/or maintenance instructions. Read the accompanying text carefully. Potential electrical hazards. Only qualified persons should perform procedures associated with this symbol. Hot surface(s) present which may cause burns to unprotected skin or to materials which may be damaged by elevated temperatures √...
  • Page 4 Steri-Cult Incubator ___________________________________________________________________________Service...
  • Page 5 Model 3307/3308 _______________________________________________________________________________ Warranty Notes Information You Should Know Before Requesting Warranty Service • Locate the model and serial numbers. A serial tag is located on the unit itself and on the operating manual shipped with the unit. • For equipment service or maintenance, or with technical or special application inquiries, contact Technical Services at 1-888-213-1790 or 1-740-373-4763 (USA and Canada).
  • Page 6: Table Of Contents

    Steri-Cult Incubator __________________________________________________________________Table of Contents Table of Contents Section 5 - Alarms ......5 - 1 Section 1 - Installation and Start-up .
  • Page 7: Section 1 - Installation And Start-Up

    Steri-Cult Incubator ____________________________________________________________Installation and Start-Up 1.3 Control Panel Keys, Displays and Indicators Section 1 - Installation and Start-up 1.1 Incubator Components Figure 1-1 Figure 1-2 Steri-Cult Incubator 1. Silence - Silences the audible alarm. • Chamber Gas Sample Port - Used for sampling chamber 2.
  • Page 8: Operation Of The Keypad

    RH Hi Alarm 2. Locate the unit on a firm level surface capable of sup- RH Relay porting the unit’s weight (340 lbs.-Models 3307 & 3308, 410 lbs.-Models 3310 & 3311) Tank Select 3. Locate the unit away from doors and windows and heat- Gas Guard ing and air conditioning ducts.
  • Page 9: Preliminary Cleaning

    The channels are stamped as to which end goes to the front of the unit. This incubator weighs 340 lbs. for Models 3307 & 3308, 410 lbs. for Models 3310 & 3311. Have suf- ficient personnel available when lifting. Lift the unit by the sides of the cabinet base to avoid plac- ing stress on the outer door hinge.
  • Page 10: Installing The Access Port Filter Assembly

    Level the unit front-to-back and left-to-right. Figure 1-10 orientation for 3307/3308 h. Connecting the Unit to Electrical Power See the serial tag on the side of the unit for electrical speci- fications or refer to the electrical schematics in this manual.
  • Page 11: Filling The Humidity Water Bottle

    Steri-Cult Incubator ____________________________________________________________Installation and Start-Up j. Connecting the CO Gas Supply Connect the incubator to a grounded, dedicated circuit. The power cord connector is the mains High concentrations of CO gas can cause asphyx- disconnect device for the incubator. Position the iation! OSHA Standards specify that employee incubator so the unit can be easily disconnected.
  • Page 12: Humidity And Loading The Chamber

    Steri-Cult Incubator _________________________________________________________________Installation and Start-Up The CO gas supply being connected should be industrial grade 99.5% pure (nominal) and should not contain siphon tubes. Install a two-stage pressure regulator at the cylinder outlet. The high pressure gauge at the tank should have 0-2000 PSIG range.
  • Page 13: Section 2 - Incubator Settings

    Steri-Cult Incubator ______________________________________________________________________Incubator Settings Section 2 - Incubator Settings It is recommended that the overtemp setpoint be main- tained at 1°C over the operating temperature setpoint. To change the Overtemp setpoint: 2.1 Incubator Start-Up 1. Press the Mode key until the Settings indicator lights 2.
  • Page 14 Steri-Cult Incubator ______________________________________________________________________Incubator Settings 2 - 2...
  • Page 15: Section 3 - Calibration

    Steri-Cult Incubator _________________________________________________________________________Calibration Section 3 - Calibration b. Calibrating the Infrared CO System All models of the Steri-Cult incubator have an infrared (IR) 3.1 Calibration Mode sensor. Infrared CO sensors are not effected by chamber atmosphere temperature or humidity. After the unit has stabilized, several different systems can be calibrated.
  • Page 16 Steri-Cult Incubator ________________________________________________________________________Calibration 3 - 2...
  • Page 17: Section 4 - Configuration

    Steri-Cult Incubator ______________________________________________________________________Configuration Section 4 - Configuration c. Setting the REPLACE HEPA filter reminder A HEPA filter replacement timer can be set for a specific 4.1 Configuration Mode amount of time, from 1 to 12 months of actual unit running time.
  • Page 18: Setting A Low Temperature Alarm Limit

    Steri-Cult Incubator ______________________________________________________________________Configuration e. Setting a Low Temperature Alarm Limit h. Setting a High CO Alarm Limit (tracking alarm) (tracking alarm) The high CO alarm limit is the deviation from the CO set- The low temperature alarm limit is the deviation from the point which will cause a high CO alarm.
  • Page 19: Setting A High Rh Alarm Limit

    Steri-Cult Incubator ______________________________________________________________________Configuration n. Disabling the Gas Guard System k. Setting a High RH Alarm Limit (tracking alarm) On units equipped with the Gas Guard option, the Gas The high RH alarm limit is the deviation from the humidity Guard system may be turned ON or OFF if it is not in use. The setpoint which causes a high RH alarm.
  • Page 20 Steri-Cult Incubator _______________________________________________________________________Configuration 4 - 4...
  • Page 21 Steri-Cult Incubator _______________________________________________________________________Configuration 4 - 5...
  • Page 22 Steri-Cult Incubator _______________________________________________________________________Configuration 4 - 6...
  • Page 23: Section 5 - Alarms

    Steri-Cult Incubator ___________________________________________________________________________Alarms Section 5 - Alarms 5.1 Alarms The Steri-Cult incubator alarm system is shown in the table below. When an alarm is active, the message appears in the LED message center. Pressing Silence disables the audible alarm for the ringback period. However, the visual alarm continues until the incubator returns to a normal condition.
  • Page 24: Temp Controller Failure Temp Cntrl Error

    Steri-Cult Incubator ____________________________________________________________________________Alarms 5.2 Temp Controller Failure TEMP CNTRL ERROR 5.6 Water Level Alarms In addition to other safety features designed into Steri-Cult When the water level in the bottle falls to approximately incubators, a thermostat is provided to monitor the cabinet tem- 3 inches (7.6cm), a visual alarm with display message “WATER perature.
  • Page 25: Section 6 - Troubleshooting

    Steri-Cult Incubator ________________________________________________________________________Troubleshooting Section 6 - Troubleshooting Certain conditions can occur in usage of this incubator which can be resolved by checking the troublshooting chart below. If the issue is not listed or cannot be resolved, contact the Services department. Problem Possible Cause Solution...
  • Page 26: Section 7 - Routine Maintenance

    Steri-Cult Incubator ______________________________________________________________________Maintenance Section 7 - Routine Maintenance 4. Wash the cabinet interior with disinfectant, starting at the top and working down. Refer to the disinfectant label for instruction on the length of time needed before rinsing. Wash the inner door(s) both inside and out. The If the unit has been in service, turn it off and dis- cabinet and door must be rinsed with sterile water until connect the power cord connector before proceed-...
  • Page 27: Cleaning The Humidity System

    Steri-Cult Incubator ______________________________________________________________________Maintenance Figure 7-4 7.3 Cleaning the Humidity System 10. Also behind the access door is the humidifier. See Figure 7-4. Make sure power is on and the RH setpoint has been Lift the latches on both sides of changed from factory default.
  • Page 28 Model 3307/3308 ______________________________________________________________________Maintenance orientation for 3307/3308 Figure 7-5 orientation for 3310/3311 7 - 3...
  • Page 29: Section 8 - Sterilization Cycle

    Steri-Cult Incubator ______________________________________________________________________Sterilization Cycle Section 8 - Sterilization Cycle Sterilization Cycle Information You Need to Know Before Starting a Cycle • If the Sterilization Cycle is to be performed on initial start-up with new unused filters, do not install the access port or HEPA filters until AFTER the Sterilization Cycle. These fil- ters should be sterilized only to decontaminate them for disposal.
  • Page 30 Steri-Cult Incubator ______________________________________________________________________Sterilization Cycle Checkpoints What if? Then Cycle does not initiate Check for alarms: or terminates in mid-cycle TEMP IS HIGH, AIR SENSOR ERROR, TEMP CNTRL ERROR Units are stacked A Sterilization Cycle performed on either unit affects performance on second unit. Do not use second unit during Sterilization Cycle.
  • Page 31: Sterilization Cycle

    Steri-Cult Incubator _______________________________________________________________________Sterilization Cycle 8.1 Sterilization Cycle 8. After ENTER is pressed with the sensor hole plugs installed, the Heat Phase initiates. The cycle initiation button flashes and the display toggles between “STER- The Sterilization Cycle will heat the incubator ILIZING”...
  • Page 32 Steri-Cult Incubator ______________________________________________________________________Sterilization Cycle 11. Completed Sterilization Cycle - When the temperature 14. Open the chamber and replace the present HEPA with a cools down to the original set operating temperature or new one. For instructions to install, see Section 1.5f. 30°C, whichever is higher, the display toggles between 15.
  • Page 33 Avoid spraying cleaner on the CO sensor. Use sterile, distilled or demineralized water. Do not use powdered gloves for tissue cultures. Millcreek Road, Box 649 • Marietta, Ohio 45750 USA • 740-373-4763 USA and Canada 888-213-1790 • Telefax: 740-373-4189 • http://www.services.controlenv@thermo.com...
  • Page 34 Incubators ______________________________________________________________________________ Preventive Maintenance Preventive Maintenance for Steri-Cult Incubators Refer to Manual Action Daily Weekly Monthly 3 to 6 Yearly Section Months Check CO tank levels. Inspect door latch, hinges and door gasket seal. * Verify and document CO , humidity and temperature calibration, as applicable.
  • Page 35: Section 9 - Factory Options

    Steri-Cult Incubator __________________________________________________________________Factory Options Section 9 - Factory Options 9.1 Connections to External Equipment a. Connecting the Remote Alarm Contacts A set of relay contacts is provided to monitor alarms through an RJ11 telephone style connector on the back of the cabinet.
  • Page 36: Co Gas Guard

    Steri-Cult Incubator ____________________________________________________________________Factory Options 191761 4-20 mA 191763 0-1V 191762 0-5V Output Scaling Output Scaling Output Scaling 4-20 mA Equals 0-1 V Equals 0-5V Equals Temperature 12.5-162.5°C 0.0-150.0°C 0.0-150.0°C 0-100 %RH 0-100 %RH 0-100 %RH 0.0-100.0 %CO 0-100.0 %CO 0-100.0 %CO Table 9-1, Analog Output Board Specifications 9.2 CO Gas Guard (1900153)
  • Page 37: Operation Of The Co Gas Guard

    Steri-Cult Incubator ____________________________________________________________________Factory Options c. Operation of the CO Gas Guard 9.3 Datalogger (P/N 201912) With the Gas Guard in operation, the incubator will use the 1. To install the datalogger sensor, locate the sensor and gas supplied by the tank connected to Inlet #1 until the pressure cable in the bottom of the incubator chamber.
  • Page 38: Section 10 - Specifications

    Shelves Standard Maximum Construction Stainless steel, perforated Clearance Adjustable on 1.160” centers Dimensions (3307/3308) 17.7” x 19.9” (45.2cm x 50.5cm) Surface area 2.4 sq. ft. per shelf Dimensions (3310/3311) 25.7” x 19.9” (65.5cm x 50.5cm) Surface area 3.6 sq. ft. per shelf Loading 50 lbs.
  • Page 39 43.0” W x 39.4” H x 27.0” F-B (109.2cm x 100.1cm x 68.6cm) Weight Net weight Model 3307/3308 - 330 lbs. (149.7kg), Model 3310/3311 - 410 lbs. (186.0kg) Shipping (motor) Model 3307/3308 - 400 lbs. (181.4kg), Model 3310/3311 - 480 lbs. (217.7kg)
  • Page 40: Section 11 - Servicing

    Shelf channel, left 250128 12V valve (RH air valve) 3310904 Diffuser pan, large (3310/3311) 360251 Door switch, outer 3307904 Diffuser pan, small (3307/3308) 360263 Door switch, inner 191682 Microboard, main control 280096 Blue LED light source 230201 Fuse, 750mA, TD, 5 x 20mm...
  • Page 41: Replacing The Fuses

    Steri-Cult Incubator ____________________________________________________________________________Servicing 11.2 Replacing the Fuses 11.3 Replacing the CO and RH Air Filters Refer to Wiring Diagram 3307-71, page 1 of 2, Fuse Holder Detail 2. The following procedure requires accessing the electronics area. Qualified personnel only should perform this procedure.
  • Page 53 The Technical Services Department must give prior approval for return of any components or equipment. At Thermo's option, all non-conforming parts must be returned to Thermo Electron Corporation postage paid and replacement parts are shipped FOB destination.
  • Page 54 The Technical Services Department must give prior approval for return of any components or equipment. At Thermo's option, all non-conforming parts must be returned to Thermo Electron Corporation postage paid and replacement parts are shipped FOB destination.
  • Page 55 Steri-Cult Incubator ___________________________________________________________________________Appendix A Appendix A...
  • Page 56 picofast “ Snap Lock” Connector Instructions UNLOCK With TURCK’s patented Locking Sleeve pulled back, any picofast sensor slides on without any difficulty. LOCK After sensor is connected, push Locking Sleeve for- ward to create a watertight connection. It’s a snap! Installation Instructions To Detach: To Attach:...
  • Page 57 Thermo Electron Corporation Controlled Environment Equipment Millcreek Road, P.O. Box 649 Marietta, Ohio 45750 U.S.A. _________________________ Telephone (740) 373-4763 Telefax (740) 373-4189...

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330833103311

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