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MultiSplit V2
Manual
The MultiSplit is calibrated and QC'd prior to delivery, however some alignment is required upon initial setup
using the calibration cubes provided (each containing a 50% mirror). This manual provides instructions for
mounting the unit on your microscope and basic alignment to project four identical images onto your camera
chip. A more detailed technical manual can also be provided upon request.
For the purposes of this manual, all text in
1) Mounting to your microscope
1. The MultiSplit is supplied with four detached support feet, these should be screwed into the mounts on the
base of the unit prior to setup. A blue locking ring allows each support leg to be locked off at the correct
position.
2. Screw your microscope c-mount onto the input section of the MultiSplit and attach to your microscope
frame.
3. Adjust the support feet until the unit is level (a spirt level is recommended).
4. Initial setup is completed without your imaging camera mounted on the unit, so for the time being, place
your camera safely to one side.
2) Key Controls
The following images show the MultiSplit panels, which will be referenced during setup of the unit.
2.1) MultiSplit Panels
Camera mounting thread
Output port
H and V adjustment for
cube 2
Figure 1: MultiSplit top panel
bold blue
is referenced on subsequent images.
H and V adjustment for
cube 3
Horizontal (H) and Vertical
(V) image separation
controls
Input port
Rectangular aperture adjusters
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Summary of Contents for Cairn MultiSplit V2
- Page 1 MultiSplit V2 Manual The MultiSplit is calibrated and QC’d prior to delivery, however some alignment is required upon initial setup using the calibration cubes provided (each containing a 50% mirror). This manual provides instructions for mounting the unit on your microscope and basic alignment to project four identical images onto your camera chip.
- Page 2 Auxiliary drop-in positions (TR) Cube 1 magnetic door cover Auxiliary drop- in positions (TT) Support leg Cube 1 H and V controls Cube 2 magnetic door cover Figure 2: MultiSplit side panel 1 Auxiliary drop-in positions (RR) Auxiliary drop-in positions (RT) Cube 3 magnetic door cover Figure 3: MultiSplit side panel 2...
- Page 3 3. Once your camera has been mounted following initial alignment, the aperture edges should be in sharp focus in your image when partially closed. If not, please contact us (tech@cairn-research.co.uk) for further advice and we will be happy to advise depending upon your hardware setup.
- Page 4 Cubes 2 and 3 (both the calibration cubes and Cairn filter cubes) will have this blue handle removed and once inserted into the cube cradles as shown in figure 6, the cradle can be inserted to positions 2 and 3 respectively.
-
Page 5: Initial Alignment
3) Initial alignment For initial setup, we suggest mounting the MultiSplit to your microscope without your imaging camera. The four quadrants can then be roughly aligned in the correct configuration, which avoids issues with some imaging cameras flipping the image in software. Final adjustments can then be made with the camera in place. To aid this initial setup, a piece of paper held over the camera mounting thread is sufficient to observe the... - Page 6 Figure 9: MultiSplit light path diagram 3.2) Positioning the primary image (TT) 1. Turn up your brightfield microscope light source to maximum and divert the light to the relevant side port. 2. Ensure no filter cubes are within the MultiSplit unit. 3.
- Page 7 4. Using the blue thumb screws labelled H and V adjustment for cube 2 in Figure 1, re-position this image to the correct quadrant. 5. When looking through the piece of paper towards the microscope from the output of the MultiSplit, move the TR image to the top left corner.
- Page 8 2mm thick dichroic mirror range. The Cairn Filter Cube accepts 26mm x 38mm x 2mm dichroic mirrors and 25mm ring mounted emission filters. Other dichroic mirrors will fit; however, they may not be optimal. We are of course happy to provide filter and dichroic mirror advice for your specific application, so please get in touch.
- Page 9 ET 445/58m 5.3) Polarisation (anisotropy) studies For polarisation experiments, a Cairn filter cube with an integrated polarising beamsplitter cube* is also available as an additional accessory. In addition, a rotating 18mm polariser is available to act as a ‘clean-up’ for the reflected S wave.
- Page 10 Figure 13: Auxiliary drop-in holder and retaining ring Neutral Density kit A series of four neutral density filters are useful if one (or more) of the channels is disproportionally brighter than another. 03GN25: 50% Transmittance 05GN25: 32% Transmittance 06GN25: 25% Transmittance 10GN25: 10% Transmittance Corrector lens kit If one or more of the channels looks out of focus in comparison to others, it may be due to chromatic aberration.
- Page 11 Corrector lens position: TR Corrector lens position: TT Cube 1 Cube 2 Figure 14: Corrector lens positions for TR and TT Corrector lens position: RR Corrector lens position: RT Cube 3 Figure 15: Corrector lens positions for RT and RR...
- Page 12 5. Ensure the Plugin is ticked as ‘Enabled’ and ‘Snap/Live’ in the On-The-Fly Processing Pipeline dialogue box. 6. Within the ‘Cairn Multi Split’ dialogue box, ensure the tab is set to ‘OptoSplit’ and the channel option is set to ‘4 Channels’...
- Page 13 10. Each individual image can be nudged using the arrows within the Multi Split window, then saved once complete. If you have any questions during installation or use of the MultiSplit, please do not hesitate to contact our Tech Support Team (tech@cairn-research.co.uk)