Leica ASP300 Instruction Manual
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Leica ASP300 Instruction Manual

Advanced smart processor automated vacuum tissue processor
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Leica ASP300
Advanced Smart Processor
Automated vacuum
tissue processor
Instruction manual
Leica ASP300
V1.3 English - 12/2003
Always keep this manual together with the instrument.
Read this instruction manual carefully
before working with the instrument.

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  • Page 1 Leica ASP300 Advanced Smart Processor Automated vacuum tissue processor Instruction manual Leica ASP300 V1.3 English - 12/2003 Always keep this manual together with the instrument. Read this instruction manual carefully before working with the instrument.
  • Page 3 Leica reserves the right to change technical We are under no obligation to update the specifications as well as manufacturing pro- present manual according to the latest tech- cesses without prior notice.

  • Page 4: Table Of Contents

    Table of contents Important notes ..........................6 Symbols used in this manual ....................6 Designated use .......................... 6 Safety .............................. 7 Safety instructions ........................7 Warnings ............................8 Integrated safety devices ...................... 10 Instrument components and specifications ................11 Specific instrument options....................11 Overview - instrument components ..................
  • Page 5 Wax clean programs ......................61 7.1.3 Smart clean ..........................62 General cleaning steps ......................63 7.3.1 Daily cleaning and maintenance ..................65 7.3.2 Periodic cleaning and maintenance ................... 66 Preventative maintenance schedule ................. 67 Warranty and service ........................ 69 Leica ASP300...

  • Page 6: Important Notes

    Important notes 1.1 Symbols used in this manual 1.2 Designated use and their meaning The Leica ASP300 is an automated tissue pro- cessor for the following laboratory applications: Warnings • fixation appear in a grey box and are marked • dehydration by a warning triangle •...

  • Page 7: Safety

    Make sure to comply with the safety instructions and warnings in this chapter. Make sure to read these instructions, even if you are already familiar with the operation and use of other Leica products. 2.1 Safety instructions This instruction manual includes important in-...

  • Page 8: Warnings

    Safety 2.2 Warnings The safety devices installed in this instrument by the manufacturer only constitute the basis for accident prevention. Primarily responsible for accident-free operation is above all the institution which owns the instrument and, in addition, the designated personnel who operates, services or repairs the instrument.
  • Page 9 Empty the condensate bottle at regular intervals. Warnings - working with the instrument The Leica ASP300 may only be operated by trained laboratory personnel and as per the present instruction manual. In case of emergency switch off mains (item no. 5 in Fig. 3) at the rear of the instrument and unplug the power cord.

  • Page 10: Integrated Safety Devices

    Safety 2.3 Integrated safety devices The instrument is equipped with the following protection features: • overpressure protection, • protection against excessive vacuum, • overcurrent protection, • overheating protection. Overpressure protection The design of the pressure system ensures that no dangerous levels of pressure can build up.

  • Page 11: Instrument Components And Specifications

    • Magnetic stirrer. • Programmable program start time delay. • Programmable finish time for processing programs. • 3-step retort drain feature. • Specimen processing under pressure, vacuum, alternating pressure / vacuum cycles or under ambient pressure. • User-programmable clean cycles. Leica ASP300...

  • Page 12: Overview - Instrument Components

    Instrument components and specifications 3.2 Overview - instrument components 12 11 8 Fig. 1 Instruction manual V 1.3 – 12/2003...
  • Page 13 15 - Reagent bottle (13 units) 16 - Drip tray 17 - Wax drain connection 18 - Remote drain connection 19 - Retort 20 - Wax baths 21 - Specimen basket 22 - Lid for specimen basket 23 - Splash protector Leica ASP300...

  • Page 14: Standard Delivery - Packing List

    Instrument components and specifications 3.3 Standard delivery - packing list Leica ASP300 basic standard delivery: 1 Leica ASP300 - Basic instrument 0476 34100 1 Accessory kit consisting of: 0476 35929 - 1 Basket hook 0476 34713 - 3 Specimen baskets, assy., including lid, dividers, springs and handle...

  • Page 15: Technical Data

    (can be operated as both normally-open and normally-closed connections) Paraffin wax baths Number of baths: Volume (liters): 4.3 l per bath Melt time: approx. 10 hrs Temperature: 40 to 65 °C Temperature accuracy: + 1 °C Leica ASP300...
  • Page 16 Instrument components and specifications Retort Capacity: max. 300 cassettes Reagent volume (in liters): Temperature (paraffin): 40 to 65 °C Temperature (processing reagents): ambient or 35 to 55 Temperature (cleaning reagents): 50 to 65 °C Temperature accuracy: + 1 K Filling time: approx.
  • Page 17 • Reagent temperature adjustable from 35 °C to 55 °C or ambient. • Paraffin temperature adjustable from 40 °C to 65 °C. • Choice of three retort drain rates of 80, 120 and 140 seconds. • Up to 100 reagent names in memory. Leica ASP300...

  • Page 18: Compatible Reagents

    1. Xylen (recommended) 2. Toluol * Prior to using these reagents, contact 3. Chloroform your Leica representative or Leica di- rectly for information on necessary pro- Paraffin wax tective measures. Using these reagents will require an increased number of main- 1.

  • Page 19: Setting Up The Instrument

    • Relative humidity maximum 80%, non-condensing. • Avoid vibrations, direct sunlight and heavy variation in temperature. The chemicals to be used in the Leica ASP300 are both flam- mable and noxious. Therefore, the instrument must be set up in a well ventilated area, free from any ignition sources.
  • Page 20 Connecting the instrument to mains The instrument MUST be connected to an earthed mains power outlet socket. It is recommended that the ASP300 be plugged into a wall socket that has Ground Fault Circuit Interruption (GFCI) protection - as an additional electrical safeguard.

  • Page 21: Installing The Accessories

    • Connect the remote fill / drain hose as shown in Fig. • Important! When inserting the hose into the fill/drain connection (item 7 in Fig. 5) the connecting device (8) of the hose must lock with an au- dible click. Fig. 5 Leica ASP300...

  • Page 22: Setting Up The Data Connections

    Setting up the instrument 4.4 Setting up the data connections Printer (optional) • Lift off the instrument console flap (12). • Connect the printer to the printer port (7) via a standard Centronics cable (refer Fig. Other connections • For additional data transfer and storage the instrument console is equipped with a stan- dard 1.44 MB floppy drive (10) for 3.5“...

  • Page 23: Alarm Functions

    A maximum voltage of 30 V DC may be present. Connecting a remote alarm device: A remote alarm device can be connected to the Leica ASP300 via a 6.3 mm-diameter jack connector (optional accessory). • For details on how to connect a remote alarm device to the Leica ASP300, please contact your local Leica office or the manufacturer Leica Microsystems Nussloch directly.

  • Page 24: Switching The Instrument On

    Setting up the instrument 4.6 Switching the instrument on Once the instrument has been switched on, the mains switch (ON/OFF) (5) should always re- main in position ON. • After switching it on the instrument will take a few minutes to initialize (see opposite - Fig.
  • Page 25 Fig. 5 - Delete all programs and reset instrument status (all lists will be empty). 6 - Store actual instrument status on floppy disk. 7 - Display run log. 8 - Restart instrument. Fig. 10 Leica ASP300...

  • Page 26: Touch Screen Functions

    To activate any functions on the Leica ASP300 touchscreen, press the corresponding button symbols. Examples of buttons are: Enabled Buttons on the Leica ASP300 touch screen can either be enabled or dis- abled. Disabled A button that is disabled appears with a thinner border than a button that is enabled.

  • Page 27: Access Control

    Setting up the instrument 4.7.2. Access control The Leica ASP300 may be configured to allow two levels of user ac- cess. ‘Operator’ symbol Operator access level: • Operators may run programs and view results. On this level, the OP- ERATOR symbol is displayed in the upper right corner of the touch screen.

  • Page 28: Check List For First Operation (At Supervisor Access Level)

    ‘REPLACE REAGENTS’ warning thresholds. Allocate a reagent to each of station. → → → Fill the reagent stations (bottles), either via the ASP300 remote drain/ fill function → (SMART SCREEN) or manually (REAGENT STATUS). Create the programs you need. →...

  • Page 29: Operation

    • On the REAGENT STATUS screen, verify correct allocation and set the reagent bottle as full. ↓ ↓ 2. Use the ‘Remote Fill / Drain’ features of the Leica ASP300 Connect the supplied remote fill/drain hose to the remote drain connection (refer Fig.
  • Page 30 Operation Fill / drain reagents → In FAVORITES, press MENU. In MENU FUNC- TIONS, press SMART SCREEN. The SMART SCREEN (Fig. 12) pops up. Fig. 12 Select station Press key symbol Remote drain • On the screen, select reagent bottle no. 3 and the bulk container (Remote).

  • Page 31: Changing The Wax

    From the FAVORITES screen, press SMART → SCREEN (see previous chapter). • Select the wax bath to be drained and the → remote container (‘Remote’). Then press STATION DRAIN. • Several queries will be displayed each of which has to be confirmed individually. Leica ASP300...
  • Page 32 Operation Filling with wax pellets • Remove the splash protector. • Fill the wax bath (Fig. 15) with wax pellets up → → to the top. • Press BACK and REAGENT STATUS to ac- cess the reagent status table (Fig. 14).
  • Page 33 • After a wax bath has been filled, it must be marked as ‘full’ in the REAGENT STATUS screen. Software control • If the wax baths are filled with molten wax, it is possible to override the Leica ASP300 melting time calculation. For that purpose, in supervisor mode, access the → →...

  • Page 34: Setting Up The Instrument Parameters

    Operation 5.2 Setting up the instrument parameters From the start screen, press MENU to go to the MENU FUNCTIONS → screen, then press MORE. In the MORE MENU FUNCTIONS screen, press SYSTEM SETUP. 5.2.1 System setup SYSTEM SETUP screen (Fig. 17). Fig.
  • Page 35 Press WAX BATH SET TEMP (°C) to access the wax bath temperature entry window. Select the highest temperature compatible with the type of paraffin used as paraffin temperature will drop slightly once the paraf- fin is in the retort. Leica ASP300...
  • Page 36 Operation Security To determine the level of access to the instrument. • SUPERVISOR PASSWORD: Enabled or Disabled Enabled - A password is required for access to the supervisor level. Disabled - All instrument and software functions are fully accessible to all staff. •...

  • Page 37: Editing The Reagent List

    • Enter the new reagent name. • Press OK to confirm. • You will then automatically be prompted to select the reagent group: Select the reagent group Allocate the new reagent to the desired group and press OK to confirm. Fig. 19 Leica ASP300...
  • Page 38 Operation Allocation of reagents to the correct reagent group is the basis for compatibility monitoring. Allocation to the wrong reagent group can lead to reagent cross contamination. Changeable parameters Entering / modifying reagent thresholds • Highlight the reagent to be modified. •...
  • Page 39 • In the reagent list, highlight the station / reagent that has been filled manually. • Press SET AS FULL to mark the station as ‘full’. ↓ Upon activation of the RMS all warning thresholds for the se- lected reagent are automatically reset to ‘0’. Leica ASP300...

  • Page 40: Viewing The Program List

    5.2.3 Viewing the program list → This list (Fig. 20) displays all programs currently existing in the ASP300. You can create: • up to 15 processing programs, • 3 retort cleaning programs, • 1 wax cleaning program. At supervisor access level: •...

  • Page 41: Adding And/Or Modifying Programs

    • duration of step (with the exception of fill and drain steps) • retort temperature (if “Ambient“ is selected, the retort temperature indication remains empty) • type of pressure and/or vacuum cycle • retort drain time • delay step. Leica ASP300...
  • Page 42 Operation Editing program steps • To edit a step, highlight the corresponding line and press the re- spective headline. • In the entry windows that pop up enter / select the program step set- tings. ↓ ↓ ↓ ↓ ↓ Fig.
  • Page 43 • Use the MOVE UP / MOVE DOWN symbols to move program steps up or down within an existing program without having to recreate those steps. DELETING PROGRAM STEPS • Highlight the step to be deleted. • Press DELETE. Leica ASP300...

  • Page 44: Favorites (Maximum Of 5 Programs)

    Operation 5.2.5 Favorites (maximum of 5 programs) Favorites can be programmed to end either: • “as soon as possible“ (ASAP) or • at a predetermined finish time. ↓ Fig. 22 Adding and/or modifying Favorites • Highlight the program line to be altered. ↓...
  • Page 45 ↓ ↓ Deleting a ‘Favorite’ • Highlight the Favorite to be deleted. • Press DELETE. The ‘Favorite“ is deleted from the FAVORITES list only - the program itself is not deleted. Leica ASP300...

  • Page 46: Editing The Stations

    Operation 5.2.6 Editing the stations Fig. 23 shows all stations / allocated reagents cur- → → rently defined in the instrument. ↓ Fig. 23 Adding / changing reagents Allocating reagents Highlight the desired station. • Only processing reagents (except paraffin) can be allocated to stations 1 –...

  • Page 47: Reagent Groups

    • Press COLOR in the table header: the entry window SELECT COLOR pops up. • Select the desired color and press OK to confirm. • If you want to close the entry field without changing the color, press CANCEL. Leica ASP300...

  • Page 48: Programs

    Operation 5.3 Programs Programs can be started from two different screens: ALL PROGRAMS FAVORITES (max. 5 programs) Fig. 25 Fig. 26 A ”Favorite” is a processing program that is In the Favorites screen, press frequently used. ALL PROGRAMS. All settings are already programmed. Only the number of blocks has to be entered (if this •...

  • Page 49: Editing A Selected Program

    To modify a program prior to starting it, press EDIT to access the Edit Program screen. Fig. 28 ↓ ↓ • Enter the day when you want the program finished. • Enter the finish time (24-hour clock format). • Press OK to confirm. Fig. 29 Leica ASP300...
  • Page 50 5. Operation Access the RUN OPTIONS function to display the options selected for the current program. • LEVEL CHECK enabled: The lower level sensor in the retort is acti- ↓ vated. • RECIRCULATION enabled: During each processing step, the reagent is pumped periodically from the bottle to the retort and back for continuous and efficient mixing of fluid.

  • Page 51: Starting A Program

    The finish time of a program in progress can only be changed as long as the delay step has not yet been completed. • Press TABLE to change from graphic to tabular program display, → press GRAPHICAL to go back to graphic display. Leica ASP300...

  • Page 52: Reagent Status

    Operation 5.4 Reagent status In the reagent status screen (Fig. 32) you can display and update the → actual reagent status of each station and you can set the stations as full or empty. • Warning thresholds that trigger warning messages appear on a red background.

  • Page 53: System Monitor

    This screen displays the average fill / drain times of all stations (in seconds). Short term = average duration of last 5 fills / drains. Long term = average duration of last 20 fills / drains. If one of the figures displayed exceeds 250, call Leica Technical Service. Leica ASP300...

  • Page 54: On-Line Help

    Operation 5.6 On-line help The Leica ASP300 features an extensive on-line Help system. For information on basic problems refer to the Help Index (to access press INDEX on the Help screen). Use the UP/ DOWN arrows to scroll. Press PREVIOUS TOPIC (1) to return to the index.

  • Page 55: Configuring A Printer (Optional)

    For the PRINT symbol to be displayed, a printer must have been configured for the instrument. The following lists and protocols can be printed: • reagent list, • stations list, • reagent status list, • all programs, • run log, • error log. Leica ASP300...

  • Page 56: Trouble Shooting

    Trouble shooting 6.1 General The Leica ASP300 is equipped with a trouble shooting system. If an error is detected, detailed error messages and corresponding user instructions on what to do are displayed on the touch screen. For all correctable error conditions the instrument will take corrective actions to protect the specimens.

  • Page 57: Trouble Shooting

    • Verify that the power switches on the rear (adjacent to the power lead) and on the side of the instrument are both switched ON. If, by carrying out the above listed steps, you cannot identify the problem, call Leica Technical Service. Leica ASP300...

  • Page 58: Typical Fill Or Drain Problems

    • Verify that both the reagent bottles and the wax stations have sufficient reagent. 2. Insufficient pressure or vacuum The Leica ASP300 is unable to create sufficient vacuum (for a fill) or pressure (for a drain) in the retort. • Verify that the reagent bottle in question is correctly inserted in the reagent cabinet.

  • Page 59: Cleaning And Maintenance

    (Fig. 35). To display a clean program, highlight the re- spective line and press EDIT. The ASP300 offers three retort clean programs and one paraffin clean program. Clean programs cannot be copied or deleted. 7.1.1 Retort clean programs A clean program comprises a...
  • Page 60 Cleaning and maintenance Editing steps • To edit a program step highlight the corresponding line and press the respective table header. • To disable a step, set the number of cycles to “0“. Only cleaning wa- ter steps can be disabled. The following characteristics of each step can be edited: •...

  • Page 61: Wax Clean Programs

    Cleaning and maintenance 7.1.2 Wax clean programs The Leica ASP300 contains a wax clean fea- ture that removes solvents (xylene) from used wax. Follow the instructions on the left (Fig. 36) to run a wax clean program. ↓ ↓ Fig. 36 The duration of the wax clean program is →...

  • Page 62: Smart Clean

    Cleaning and maintenance 7.1.3 Smart clean The Smart Clean prepares the ASP300 for transport. Prior to starting a Smart Clean, a complete retort clean program must be run. To start a Smart Clean, press SMART CLEAN on the MORE MENU FUNC- →...

  • Page 63: General Cleaning Steps

    Clean the instrument exterior as necessary. Wipe with a damp cloth moistened with mild detergent and dry. Do not use solvents on painted surfaces and/or the touch screen! Checking the drip tray Regularly check the drip tray for spilled reagents (refer Fig. 38). Fig. 38 Leica ASP300...
  • Page 64 Cleaning and maintenance Draining the condensate bottle • Remove and empty the condensate bottle and reinsert (Fig. 39). Fig.39 Exchanging the activated carbon filter • Exchange the filter as shown in Fig. • Replace the filter at least every four months –...

  • Page 65: Daily Cleaning And Maintenance

    • To ensure easy removal of the reagent and condensate bottles, lu- bricate the O-ring seals on the plug-in nozzles with the O-ring lubri- cant supplied. • Check regularly whether the reagent bottles are still properly con- nected to the connection manifolds in the instrument. Retighten, if necessary. Leica ASP300...

  • Page 66: Periodic Cleaning And Maintenance

    7. Cleaning and maintenance This procedure is particularly important with bottles containing chloroform. Chloroform tends to swell the bottle seals, making them difficult to remove. This problem can be prevented by regular lubrication. Checking the retort and wax bath lid seals •...

  • Page 67: Preventative Maintenance Schedule

    √ Inspect and, if necessary, clean wax bath lid seal. √ Inspect and, if necessary, adjust paraffin levels. √ Inspect reagent container fluid levels. if the automatic fill and drain function is actually being used Leica ASP300...
  • Page 68 Cleaning and maintenance Maintenance activity daily weekly monthly every 4 months √ Inspect and, if necessary, clean wax bath strainers . √ Inspect wax bath air vent and, if necessary, clear. √ Inspect and empty condensate bottle. Clear inlet holes. √...

  • Page 69: Warranty And Service

    Leica Microsystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality con- trol procedure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or agreed characteristics warranted.
  • Page 70 Notice Instruction manual V 1.3 – 12/2003...

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