Biobase BK-S360 User Manual
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Biobase BK-S360 User Manual

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UV/VIS SPECTROPHOTOMETER
BK-S360
User Manual
BIOBASE GROUP
Version 2020.11

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Summary of Contents for Biobase BK-S360

  • Page 1 UV/VIS SPECTROPHOTOMETER BK-S360 User Manual BIOBASE GROUP Version 2020.11...

  • Page 3: Table Of Contents

    Content Preface..............................2 1. Safety............................2 2. About the Instrument........................2 3. Symbols............................2 I. Summary............................. 3 1. Working Principle and Structure....................3 2. Main Specifications........................3 3. Main Functions..........................4 II. Install Instrument..........................5 1. Environment Required........................ 5 2. Voltage Confirmation........................5 3.

  • Page 4: Preface

    Preface 1. Safety This instruments are designed according to the following safety documents: 《 IEC 61010 — 1： 2001》 (Which is： 《Measurement, Control and Laboratory Electric Equipments Safety Requirement》 Part 1) and 《IEC 60601 — 1：1988》(Which is： 《Electric Apparatus of Medical Treatment》 Part 1). 2.

  • Page 5: Summary

    I. Summary Scanning Series UV/Vis Spectrophotometer have the characters of wide range of wavelength, high sensitivity, powerful function, easy to use, simple structure and pretty figure. Besides these, the large LCD, High Precise A/D and easy to store RAM makes the instrument much more superior than other originals.

  • Page 6: Main Functions

    3. Main Functions Scanning Series UV/Vis Spectrophotometer has functions below as main three parts. Photometry  The photometric mode can be switched between Absorbance, Transmittance and Energy. You can use single-point method to measure the concentration. Quantitative  Coefficient Method, Standard Curve Method and Input Method. The Regression Equation and the test result can be stored in the RAM and printed out.

  • Page 7: Install Instrument

    II. Install Instrument 1. Environment Required To ensure the best performance, the following conditions are required: The best working temperature range is 16-35℃and the humidity is 45-80%.  Keep it as far as possible away from the strong magnetic or electrical fields or any electrical ...
  • Page 8 Fig. 2-1 Fig. 2-2...

  • Page 9: Introduction Of The Instrument

    III. Introduction of the Instrument 1. Introduction Appearance of the instrument (Fig. 3-1)： Fig. 3-1 1 — Lid of the room 2 —Operation Panel 3—Cell Holder 4— Rod 5— Fan 6— Fan Cover 7— Power Socket 8— Con-Tem Port 9— Power Switch 10—USB 11—Print Port 12 —USB Print Port...

  • Page 10: Operation Panel

    2. Operation Panel Operation panel of Scanning UV/Vis Spectrophotometer(Fig.3-2)： Fig. 3-2 1 —LCD Displayer 2 —Keypad 3. Keypad Description … Input Parameters, Wavelength and set 8 cell auto cell holder. Delete the input value or stored data. Cancel operation or return to former menu Calibrate 100%T/0Abs Load the files saved in the RAM Save the test results.
  • Page 11 Set Wavelength Print Test Result Confirm the input value or setting Scroll the menu or data Seek wavelength peak or set coordinate.

  • Page 12: Instrument Operation

    IV. Instrument Operation 1. Software System Software System of Instrument (Fig. 4-1) Fig. 4-1...

  • Page 13: Basic Operation

    2. Basic Operation (1) Select Test Method In the main menu, press numeric key —— to enter this mode directly. (2) Set Wavelength Press to set wavelength,use numeric key to input the value and press confirm. When the wavelength changed to the point you set, the system calibrates 100%T/0Abs automatically. (3) Set Parameters In different menus, press to go into different parameter setting interface, input the values by...

  • Page 14: Before Measurement

    (11) Load the Stored File In the test interface, press to go into file selecting interface, press to choose the 、 file you want, press to open. In different menu, users can only load the files with the corresponding postfix. List is the corresponding postfixes: Quantitative：...
  • Page 15 Step 2, Set Test Mode Press to set up (Fig. 4-2). Press to go into selecting mode, Press 、 choose “TRANS”,“ABS” or “ENERGY” mode, and press to confirm. Press to set coefficient K (-9999.9-9999.9) to confirm. Press to confirm all settings. Step 3, Set Wavelength Press to set wavelength, input the value by the numeric keypad followed with...
  • Page 16 Fig. 4-4 Step 2, Set Parameters Press to enter the quantitative setting interface (Fig. 4-4). Press numeric key to select. Press to confirm. Press to enter standard sample test, input the standard sample concentration, press to confirm. After finished setting, press to test successively or press to input standard sample Abs.
  • Page 17 Quantitative Method. Press to choose standard sample read (RECORD). to confirm. Set Parameter. According to the menu options, press numeric key to set up fitting method, wavelength method, units and room, press to confirm, then press to enter wavelength, sample no., to confirm.
  • Page 18 4) Set Standard Sample. According to the notice, input the standard sample concentration (Fig. 4-7), to confirm, press to select the value. After finishing inputting concentration, press to enter Abs. INPUT, the method is same as inputting concentration. Then press to enter curve picture, press again to enter quantitative data sheet to start test.
  • Page 19 Fig. 4-7 Load the Stored Curves： In the “Quantitative” interface,press to go into files selection interface.Use select the curve you need and press to load. Users can press to check the curve.(Fig.4-5),press to return. Step 4,Go into the sample measurement interface In the “Quantitative Curve”...
  • Page 20 Step 7, Print Data Press to print the result. Step 8, Delete Data Press to move the cursor to select the value you don’t need, then press to delete the value. Step 9, Store the data After measurement, press to store the data. Input the file name by the numeric keypad and press to store.
  • Page 21 Press numeric key to input interval, the data in the data sheet read according to the interval. Press numeric key to input the sample Abs. range, which is -4-4. Press numeric key to choose room, standard is manual, default is auto. After finished, press to enter spectrum interface.
  • Page 22 Step 6,Print curve Press to print curve and data sheet. Step 7, Save and Open After scan, press to save curve, parameters and data sheet, press numeric key to input file name, to save. to open stored file. to choose file, open.
  • Page 23 Press to save data, press to choose store data, press again to input file name, to confirm.Press to choose stored file, to open and test directly. to print the tested data. Fig. 4-11 Step 6, Delete Press to delete the current data. (5) Spectrum Step 1, Go into spectrum scan In the main menu, press numeric key...
  • Page 24 Press numeric key , source light: Auto, W lamp, D lamp, press to choose test source light. Press to confirm. Press numeric key , set interval:0.1, 0.2, 0.5, 1, 2, 5nm, press to choose scan interval. Press to confirm. Press , input scan range: 190-1100nm, input the range under the practical meet.
  • Page 25 Step 5, Data Process After scan, press to enter data process (Fig. 4-16b). Press numeric key , recover original spectrum; Press numeric key to change X,Y coordinate. Press numeric key to enter data sheet. Step 6, Print Curve and Peak Value Press to print curve and data sheet.

  • Page 26: Tools

    Step 3, Setup Press to set coefficients, input all the values of 1-5 by numeric keypad according the indication and press to confirm. Press to enter test interface. Step 4, Calibrate 100%T/0Abs Place the Reference Sample in the light path, press to calibrate 100%T/0Abs.
  • Page 27 2. D2 Lamp Press anduse to choose “D Lamp on or off” then press to confirm. Users can switch Off the D2 Lamp when test in the wavelength range of 340-1100nm, so as to prolong the life time of D2 lamp. After some period of usage(About 1000h), the energy would drop off to a low level,and the test data in the UV range would get instable and deviate a lot from the experiential value.

  • Page 28: Instrument Maintenance

    V. Instrument Maintenance To keep the instrument work in good condition, constant maintain is needed. 1. Daily Maintain (1) Check the Room After measurement, the cuvettes with sample solutions should be taken out of the compartment in time. Or the volatilization of the solution would make the mirror go moldy. Users must pay more attention to the corrosive sample and liquid easy to volatilize.

  • Page 29: Spare Parts Replacement

    Cuvettes polluted Clean the cuvettes  (6) Incorrect Reading Possible Reason Solution Dark Current Error Re-get the dark current  Worse matching of the cuvettes Improve the matching of the cuvettes  3. Spare parts replacement (1) Replace the Fuse Danger! Be sure to switch off the power and unplug the socket before replacement! Step 1, Tools Preparation Prepare a 3×75 Flat Blade screwdriver.
  • Page 30 Fig. 5-2 Step 5, Reset the Fuse Seat Replace the fuse seat in the power socket. Step 6, Switch On the Power Plug the socket and switch on the power. (2) Replace Lamps High temperature！ Wait 20 minutes before open the lamp chamber after power off to avoid scald! Step 1, Tools Preparation Prepare a 6×150mm Cross Blade screwdriver and a pair of glove.
  • Page 31 Fig. 5-4 Step 5, Replace the D2 Lamp Unscrew the 2 screws on the D2 Flange (No.1 in Fig. 5-5), unplug the connector in the power board(No. 2)and remove the D2 lamp. Draw on the cotton glove and replace a new lamp. Fix the 2 screws and plug the connector again.
  • Page 32 Fig. 5-6 Step 7, Adjust the Position of the W Lamp Switch on the power,(the Switch Mirror should be placed to the position as Fig. 5-7 indicates). Observe the entrance facula,and it should in the center of the entrance hole(Fig.5-7). If the facula deviate to Left or Right, then loosen the No.1 screws in Fig.
  • Page 33 Fig. 5-8 Step 8, Finish Reset the cover of the light chamber and fix the screws. Reset the cover of theinstrument and fix the screws. Recover the Pole in the compartment, then the course finished. (3) Replace the Battery Be sure to switch off the power supply and unplug the socket before open the Bottom Cover！...
  • Page 34 Fig. 5-10 Step 5, Finish Recover the bottom plate and fix the 13 screws, then the course finishes.
  • Page 35 Appendix 1: Consumable Sheet Deuterium Lamp Tungsten Lamp 10mm Cuvette Glass (4PCS/BOX) 10mm Cuvette Quartz (4PCS/BOX)
  • Page 36 BIOBASE GROUP 2# building, No.9 Gangxing Road, High-tech Zone, Jinan City, Shandong Province, China Tel: +86-531-81219803/01 Fax: +86-531-81219804 Inquiry: export@biobase.com Complaints: customer_support@biobase.cc After-sales service: service_sd@biobase.cc; service_ivd@biobase.cc Web: www.biobase.cc/www.meihuatrade.com / www.biobase.com...

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