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miniRaman spectrometer
User Manual
English
version 202202
lightnovo.com

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Summary of Contents for Lightnovo miniRaman

  • Page 1 User Manual English version 202202 lightnovo.com...
  • Page 2: Table Of Contents

    Table of Contents Safety ......4 ..... 4 General Information .
  • Page 4: Safety

    Any non-observance of the precautions will infringe the intended use (i.e. performing measurements by Raman spectroscopy) of miniRa- man spectrometer. In this case Lightnovo ApS will not assume any liability. It is the operator’s duty to plan and implement all necessary safety measures and to supervise their observance.
  • Page 5: Warning Labels

    Safety Warning labels This warning symbol indicates the existence of laser radia- tion. A Class 3R laser is considered safe if handled carefully, with restricted beam viewing. With a class 3R laser, the maxi- mum permissible exposure (MPE) can be exceeded, but with a low risk of injury.
  • Page 6: Laser Safety

    Laser Safety General Information The analysis system miniRaman microscope uses the light of a lasers (660 and 785nm) that are installed in miniRaman spectrometer. The used laser diodes emit visible and partially visible laser radiation in the near infrared region.
  • Page 7: Installation

    Warning: Do not put miniRaman spectrometer into operation if there are signs of damage. Fail- ure to do so may result in severe personal injuries and/or property damage. Transportation...
  • Page 8 Installation Space Requirements miniRaman spectrometer requires a space of 12cm in diameter and 4cm in height. (For the exact instrument dimensions refer to Specification.) At the rear instrument side, take a clearance of at least 3cm into account. Note: MiniRaman spectrometer is an instrument of protection class I (electric safety).
  • Page 9 Installation Figure 3. Rear side – overview of the USB connector socket Download miniRaman microscope software package from Lightnovo website: https://lightnovo.com/lightnovo-software/ Please select version 32 or 64 bit depending on your operation system. Install all drivers from the corresponding software folder.
  • Page 10: Overview

    SERS signals on plasmonic substrates, identification of pow- ders and liquids. MiniRaman spectrometer is also suitable for samples that tend to fluoresce when ex- posed to laser radiation. Due to the usage of a 785nm laser, the excitation energy is low enough for these samples not to fluoresces or only to a minor degree.
  • Page 11 Overview Lasers Depending on the selection of the miniRaman spectrometer model miniRaman spec- trometer could be configured in the following models with different laser parameters: • MiniRaman spectrometer Standard (includes one laser with optical isolation: 785 nm, power range on a sample 5-50 mW) •...
  • Page 12: Instrument Design

    Overview Instrument design Key system components are shown on Figure 4. Figure 4. Optical schema of MiniRaman spectrometer. See details for patent # US20210072158 here: https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2019145005. laser – collimation lens – Wollaston unit – laser line filter – focusing lens –...
  • Page 13 Overview MiniRaman spectrometer has multiple control elements: Laser with wavelength 785nm (ON/OFF and power control) Laser with wavelength 660nm (ON/OFF and power control) Spectroscopic sensor for Raman spectra acquisition (exposure, gain, row selec- tion and binning control)
  • Page 14: Operation

    Warning: Do not work with miniRaman spectrometer at laser power that is high than specified for particular model. This could lead to the decreased life time of the laser diode or laser damage...
  • Page 15 Operation Figure 5. miniRaman spectrometer software interface; connection of accessories Figure 6. Tools menu Turn ON/OFF lasers Figure 7. Switch on/off laser Press “On” or “Off” bottoms, see below.
  • Page 16 Operation Measuring the Raman spectrum of the sample Raman spectra shown in the “Plot” window, see below. Figure 8. Curve context menu If laser is ON system should show the “live” spectrum, see below. Spectrum scale can be adjusted by right click of the mouse and selection of “fit to window” bottom. Figure 9.
  • Page 17 CMOS sensor and exposure time can be adjusted. Icon “Wait stable ref” should be applied if experiment require high resolution of Raman spectra. Figure 12. miniRaman microscope software interface; calibration of 3D stage Figure 11. miniRaman spectrometer software interface; advanced settings menu Figure 12.
  • Page 18 Operation Figure 14. Spectrum capture Spectra preprocessing Figure 15. Options menu Figure 16. Smoothing methods Figure 17. Background correction methods...
  • Page 19 Operation Saving and/or exporting the data Export spectrum in tsv: Menu/file/save (see Figure below). Figure 16. Spectrum capture Figure 18. Saving captured spectrum GUI settings It is possible to optimize user interface based on customer preferences. Windows can be adjusted with docking widgets, see below. Figure 19.
  • Page 20 Operation Each user interface can be saved, see below. Figure 20. Adding perspective Figure 21. Saving perspective Saved perspective can be opened at any time later, see below. Figure 22. Saved perspective list...
  • Page 21: Technical Drawings

    Technical drawings Figure 23. miniRaman spectrometer drawing with dimensions...
  • Page 22: Service Addresses

    Service addresses Lightnovo ApS Blokken 15, 1. Tv. 3460 Birkerod Denmark Phone: +45 71370410 https://lightnovo.com/ service@lightnovo.com...

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