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Related Manuals for Lightnovo miniRaman
Summary of Contents for Lightnovo miniRaman
- Page 1 User Manual English version 1.0 lightnovo.com...
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Page 2: Table Of Contents
Software Installation ..12 Removal and installation of miniRaman spectrometer Overview ......13 . -
Page 4: Safety
Any non-observance of the precautions will infringe the intended use (i.e. performing measurements by Raman spectroscopy) of miniRaman microscope. In this case Lightnovo ApS will not assume any liability. It is the operator’s duty to plan and implement all necessary safety measures and to supervise their observance. -
Page 5: Warning Labels
Safety Warning labels This warning symbol indicates the existence of laser radia- tion. A Class 3R laser is considered safe if handled carefully, with restricted beam viewing. With a class 3R laser, the maxi- mum permissible exposure (MPE) can be exceeded, but with a low risk of injury. -
Page 6: Laser Safety
Laser Safety General Information The analysis system miniRaman microscope uses the light of a lasers (660 and 785nm) that are installed in miniRaman spectrometer. The used laser diodes emit visible and partially visible laser radiation in the near infrared region. - Page 7 Safety Safety Instructions In additions to the safety instructions given below, also comply with all local regulations concerning laser safety. The analysis system is specified as a laser class 3R/3B product (depending on the model, see overview section), i.e. it considered safe if handled carefully, with restricted beam viewing.
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Page 8: Installation
Warning: Do not put miniRaman microscope into operation if there are signs of damage. Failure to do so may result in severe personal injuries and/or property damage. Transportation... -
Page 9: Site Requirements
Installation Site requirements Space Requirements miniRaman microscope requires a space of 17cm in diameter and 28cm in height. (For the exact instrument dimensions refer to Specification.) At the rear instrument side, take a clearance of at least 5cm into account. -
Page 10: Cable Connections
Cable Connections Please unpack miniRamaman microscope and remove it from the transportation case (see Figure 3). Figure 3. miniRaman microscope with transportation case. Please remove power adaptor (Figure 4) and connect it to the right connector on the rear panel of the miniRaman microscope (Figure 5). -
Page 11: Software Installation
PC via USB cable, right connector is for power supply 12V). Software Installation Download miniRaman microscope software package from Lightnovo website: https://lightnovo.com/lightnovo-software/ Please select version 32 or 64 bit depending on your operation system. Install all drivers from the corresponding software folder. -
Page 12: Removal And Installation Of Miniraman Spectrometer
6. Removal steps of the miniRaman spectrometer from the miniRaman microscope... -
Page 13: Overview
SERS signals on plasmonic substrates, thin sample films, nano particles and surfaces. MiniRaman microscope is also suitable for samples that tend to fluoresce when ex- posed to laser radiation. Due to the usage of a 785nm laser, the excitation energy is low enough for these samples not to fluoresces or only to a minor degree. -
Page 14: Specification
Overview Specification Lasers Depending on the selection of the miniRaman spectrometer model miniRaman micro- scope could be configured in the following models with different laser parameters: • MiniRaman microscope Standard (includes one laser with optical isolation: 785 nm, power range on a sample 5-50 mW) •... - Page 15 Overview Spatial resolution • Diffraction limited (microscope objective dependent) • 600 nm (lateral) at 660 nm excitation at NA=0.95 (100x) • 750 nm (lateral) at 785 nm excitation at NA=0.95 (100x) White light illumination • Reflected light microscopy with simultaneous laser spot visualization and Raman spectra registration •...
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Page 16: Instrument Design
MiniRaman microscope consists of three key units (see Figure 7): miniRaman spectrometer white light microscopy unit motorized 3D stage MiniRaman spectrometer can be installed and taken out by the user if necessary as shown in Figure 6. Key system components are shown on Figure 7. LED for reflected white... -
Page 17: General Overview Of Control Elements And Components
Overview General overview of control elements and components MiniRaman microscope has 6 control elements: Laser with wavelength 785nm (ON/OFF and power control) Laser with wavelength 660nm (ON/OFF and power control) Spectroscopic sensor for Raman spectra acquisition (exposure, gain, row selec-... -
Page 18: Operation
Power cable (12V) Both cables could be connected (order does not matter). User should wait for LED 1 shining “Blue” (constant) and miniRaman spectrometer bottom blinking “Blue”. Then software is ready for connection (“Connect” bottom in Miraspec). After connection LED 2 should be “Green”. -
Page 19: Operation Procedure
Operation procedure Switching the system ON/OFF It is highly recommended to switch off the miniRaman microscope LEDs for white light microscopy when they are not used for a longer period of time (several hours) and overnight. This action will prolong the service lifetime of the microscope LED. - Page 20 Operation Turn ON/OFF lasers Press “On” or “Off” bottoms, see below. Figure 10. Switch on/off laser Figure 11. Tools menu...
- Page 21 Calibration of the motorized stage Open Menu/Tools/Visible microscopy and press Home bottom (see Figure 12). Figure 12. miniRaman microscope software interface; calibration of 3D stage Correcting the measuring position in case of a slight offset of the point of incidence of the laser beam, i.e. offset with regard to the cross-hairs center (If this is not the case, skip this step.), see Figure 12.
- Page 22 Spectrum will be acquired by pressing “Capture” bottom. Acquisition parameters like laser power, gain of CMOS sensor and exposure time can be adjusted. Icon “Wait stable ref” should be applied if experiment require high resolution of Raman spectra. Figure 15. miniRaman microscope software interface; laser settings...
- Page 23 Operation Figure 16. Spectrum capture Defining Raman mapping parameters, see Figure 17. Figure 17. miniRaman microscope software interface; Raman mapping settings...
- Page 24 Raman intensity fluctuations during mapping can be visualized by map of reference channel, see below. Figure 18. miniRaman microscope software interface; Raman map of the in-build reference channel Measuring the Raman map of the sample Press “Start” bottom in order to initiate the mapping process. If necessary, options of pause, resume and abort of the mapping process are also available.
- Page 25 Raman mapping parameters before acquisition. Export map in Matlab: Menu/advanced tools/scan map/export (see Figure below). Figure 20. miniRaman microscope software interface; data export. GUI settings It is possible to optimize user interface based on customer preferences. Windows can be adjusted with docking widgets, see below.
- Page 26 Figure 23. Saving perspective Figure 24. Saved perspective list System indicators. miniRaman microscope has in-build humidity and temperature sensor, see sensor posi- tion on Figure 8. Values are shown in connection status bar, see Figures below.
- Page 27 Operation Figure 25. miniRaman microscope software interface; hardware connection status and in-build sensor data. Figure 26. Connection status bar Connection status also represents the COM port number and current position of 3D stage in XYZ axis. If XYZ stage requires calibration, those values will be highlighted in yellow, see below.
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Page 28: Upright And Inverted Operation
Operation Upright and Inverted operation miniRaman microscope can be used in upright and inverted configurations without any hardware modifications. In order to switch between different operation modes it needs to be rotated as shown on Figure 28. Figure 28: a) Upright operation... -
Page 29: Technical Drawings
Technical drawings 130.00 29.39 DRAWN 11/24/2021 olil Lightnovo ApS CHECKED TITLE miniRaman microscope APPROVED SIZE DWG NO Cage housing with electronics and optics SCALE 1 : 1 SHEET Figure 29. miniRaman microscope drawing with dimensions... -
Page 30: Service Addresses
Service addresses Lightnovo ApS Blokken 15, 1. Tv. 3460 Birkerod Denmark Phone: +45 71370410 https://lightnovo.com/ service@lightnovo.com...
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