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Diagnosys Celeris User Manual

Next generation rodent erg testing
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CELERIS USER MANUAL
US: Diagnosys LLC USA ● 55 Technology Drive, Suite 100 ● Lowell, MA 01851 USA ● Phone: 978.458.1600 ● Fax: 978.458.1755 ● Email:
sales@diagnosysllc.com
UK: Diagnosys LTD UK ● 5 Trust Court, Vision Park ● Histon, Cambridge, CB24 9PW ● Phone/Fax: +44 (0) 1223 520699 ● Email:
mail@diagnosysuk.co.uk
www.diagnosysllc.com

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  • Page 1 CELERIS USER MANUAL US: Diagnosys LLC USA ● 55 Technology Drive, Suite 100 ● Lowell, MA 01851 USA ● Phone: 978.458.1600 ● Fax: 978.458.1755 ● Email: sales@diagnosysllc.com UK: Diagnosys LTD UK ● 5 Trust Court, Vision Park ● Histon, Cambridge, CB24 9PW ● Phone/Fax: +44 (0) 1223 520699 ● Email: mail@diagnosysuk.co.uk...

  • Page 2: Table Of Contents

    Setting up for a test ......................................7 Placing the Electrodes ....................................8 Setup Tips and Tricks ....................................10 Runtime Menu Controls / Celeris Control Buttons ........................12 Running a Test....................................... 14 In-test Troubleshooting .................................... 14 Printing, Saving, and Exporting Data ..............................16 Loading Saved Tests ....................................

  • Page 3: Introduction

    Celeris User Manual Introduction The Celeris system uses hardware and software developed entirely by Diagnosys LLC to provide a compact, transportable system for recording any conceivable ERG or VEP test. Diagnosys’ industry-leading, patent- pending combined stimulator-electrodes are capable of performing any electrophysiology test, even those previously possible with only custom-built lab equipment such as Scotopic Threshold responses, intensity series, Photopic Negative ERGs, or pattern stimuli.

  • Page 4: Disclaimer

    The Diagnosys Electrophysiology System software is licensed, not sold. You may install and use one copy of the Diagnosys Electrophysiology System software on a single Celeris System console. You may not install it on any additional consoles or computers. You may make one copy of the Diagnosys software solely for backup purposes.

  • Page 5: Console Buttons And Status Lights

    Figure 1 - Console control buttons and status lights Dimensions and Weight Celeris (height to table top): 15.7 x 6.9 x 5.3 inches (399 x 175 x 135 mm) Celeris heating table: 6.1 x 4.0 inches (155 x 102 mm) Celeris weight: 10 lbs (4.5 kg)

  • Page 6: Power

    The system is designed to be operated in a research laboratory under normal ambient temperature and humidity conditions. Although no special electrical shielding is required, the Celeris should not be placed in an electrically noisy environment; large machinery such as cage washers, heavy-duty refrigeration equipment, or elevator motors may introduce electrical interference into the signal.

  • Page 7: Instructions For Use

    Cover or turn off any light sources in the room. Light sources covered with red filters may be used but may affect dark adaptation, due to the wide passband of many filters. The room should be darkened. Ensure that the Celeris is turned on and that the heating pad has been permitted to come up to temperature.

  • Page 8: Placing The Electrodes

    Protocols labelled TOUCH/TOUCH ™ TOUCH/TOUCH protocols are protocols that take advantage of the patent-pending stimulator/electrode combo unique to the Celeris. TOUCH/TOUCH protocols stimulate one eye at a time and use the fellow, unstimulated eye as the reference. To set up an animal for a TOUCH/TOUCH protocol, place a small amount of hypromellose solution on the cup of each electrode, then place an electrode on each cornea.
  • Page 9 Figure 9 - The TOUCH/TOUCH Setup with gas nose cone When plugging a TOUCH/TOUCH protocol into the Celeris, imagine bisecting both the rodent and the Celeris along the midline of the animal, as seen in Figure 8 below. Every plug/input on the right side of the animal should stay on the right;...

  • Page 10: Setup Tips And Tricks

    While there is no electrophysiological advantage to either site, Diagnosys recommends placing the electrode on the hindquarters near the tail, as the skin of the tail is typically tougher and will blunt an electrode more quickly.
  • Page 11 Celeris User Manual Full-Field Stimulator electrode • Make sure that the eye has been wetted before placing the electrode. • Be sure to make contact with the cornea – making contact solely through the wetting solution will decrease amplitudes. •...

  • Page 12: Runtime Menu Controls / Celeris Control Buttons

    (target temperature is 37 °C, ±1) and which eye is being tested by this step of the protocol. A small lightbulb icon at the top controls the safety light on the Celeris. The Navigation Bar at the top controls the popout menu at the right. Pressing any of those buttons will cause the corresponding popout menu to appear;...
  • Page 13 The Control Bar at the bottom of the page controls the test. Most of these buttons have their functions duplicated by the control buttons on the Celeris. By default, buttons that can be pressed are green; buttons that are active are red; buttons that cannot be used at the moment are greyed out. Figure 12 below shows a Control Bar in the ready state;...

  • Page 14: Running A Test

    Celeris is plugged into is grounded. If using a laptop, tablet, or other battery- powered computer to drive the Celeris, try unplugging the computer from the wall to see if it is producing the noise. To enable the mains/line filter, press the Channels/Eye button at the top of the runtime menu and toggle the 50/60 Hz Line Filter from the popout menu that appears.
  • Page 15 – try testing the next animal to see if the problem persists. If the larger response switches to the other eye, contact Diagnosys for further troubleshooting. Problem: Test is running and running but doesn’t appear to record anything If the test is running and does not appear to be progressing, the auto-reject window may be enabled and set too tightly.

  • Page 16: Printing, Saving, And Exporting Data

    Celeris User Manual Figure 24 - automatic rejection parameters Printing, Saving, and Exporting Data Your data is being saved to the hard drive during testing. To save data to the database, press the Exit button at the bottom right at the end of the test.

  • Page 17: Loading Saved Tests

    Celeris User Manual Stimulus Table: Provides information about stimulus parameters for the test. Data Table: Provides X and Y axis values for each data point on the waveform. Separator: When exporting to a file, data delimiter can be Tab, comma, colon, semicolon, or space.

  • Page 18: Creating And Modifying Protocols

    Diagnosys. Diagnosys recommends calibrating stimulators on an annual basis. To clean the Celeris console, spray with isopropyl alcohol or other disinfection solution and wipe dry. Alternatively, disinfectant wipes may be used. Caution: improper cleaning may lead to damage of the electrode!

  • Page 19: Local Configuration Settings

    Configure System, accessible from the launch page of the software, permits operators to customize the configuration of their Celeris system. Most of the Configure System parameters are factory settings, and users should not change settings they are unsure of. Local configuration settings are stored in the ESLOCAL.INI file in the Celeris software directory, and Diagnosys recommends making a backup of this file before making any changes to Configure System parameters.
  • Page 20 Field 4; User Field 5; User Field 6; User Field 7; User Field 8; User Field 9. Many of these fields are suppressed in the Celeris configuration. The underlined list of codes are visible from the subject selection page, and will be the most useful for the majority of users.

  • Page 21: Tips And Tricks

    Configure System > Program > Global with the parameters Autobackup Enabled and Autobackup Directory. Diagnosys strongly recommends leaving the autobackup enabled and setting the directory to a location not on the computer’s hard drive. External hard drives or USB keys are highly recommended.
  • Page 22 0 and 40 ms. Figure 34 - marker placement settings Networking Databases There are several reasons you might want to put your Celeris database on your network: • to allow multiple Diagnosys systems to share a single database •...
  • Page 23 Celeris User Manual Export Lists of Patients/Tests You can export lists of tests or subjects tested. From the subject selection page, selecting a subject category, or typing in two dates in MM/DD/YYYY MM/DD/YYYY format will filter the list of patients to only those patients assigned to that category, or who had tests performed between those two dates.

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