Bright Instruments 8000 Manual

Bright Instruments 8000 Manual

Retracting sledge microtome

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8000 Retracting
Sledge Microtome
Manual
Instruments

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Table of Contents
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Summary of Contents for Bright Instruments 8000

  • Page 1 8000 Retracting Sledge Microtome Manual Instruments...
  • Page 2: Table Of Contents

    Knife guards Figure 2. Bottom View of Microtome Side pillars [Fig.1 13 & 16] Figure 3. Knife Holder Figure 4. Setting Blade Angle OPERATION Operating handle (3) Thickness control (5) Coarse advance/trim control (4) Specimen holder Knife clamps Bright Instruments...
  • Page 3: Safety Warnings

    SAFETY WARNING Low temperatures are present in this equipment. Extreme care should be taken. DO NOT let bare skin come into contact with metal surfaces. Bright Instruments...
  • Page 4: Knives / Blades

    SAFETY WARNING Extremely sharp knives/blades. Use knife/blade guards at all times. Use correct tools for removal and insertion of knives/blades. DO NOT leave knives/blades laying around. Place knives/blades not in use, into box/wallet provided. Bright Instruments...
  • Page 5: Safety Information

    Your attention is therefore drawn to the towards the back of this instruction manual following precautions: e. REMEMBER that even used knives and for further details. blades are dangerous. The are still sharp and may have been used to cut potentially infectious specimens. Bright Instruments...
  • Page 6: Product Safety Suggestions

    If the instrument or any part of it has been payable by us, which will be invoiced to exposed to or been in contact with potential the sender. pathogenic or radioactive material, it is essential that it be decontaminated. Bright Instruments...
  • Page 7: Warranty

    Seller will repair or replace them free by law as to the quality or fitness for any of charge and will return them to the Buyer, particular purpose of such goods. carriage paid. Bright Instruments...
  • Page 8: Introduction

    1.1.3 PACKING All packing must be carefully removed and parts checked against the enclosed packing list. If any damage or discrepancy is noted, please inform our agent/distributor, or Bright Instruments...
  • Page 9: Operating Instructions

    2.2 THICKNESS CONTROL [FIG. 1.5] clamp levers [10] and moving knife to slew A knob, calibrated in microns, is used to angle required. select the thickness of sections to be cut from 1µm – 40µm, in 1µm steps. Always set the Bright Instruments...
  • Page 10: Operation

    20µm, and move the the ends of the knife. Set the knife to the on other parts during sectioning. sledge back and forth repeatedly to trim desired cutting angle and tighten the knife the specimen. clamping levers. Bright Instruments...
  • Page 11 A slow, steady cutting action is needed to and flatten them. They can be collected on obtain the best results. However, different microscope slides as follows: specimens may benefit from different cutting speeds, so experimentation will sometimes bring improved results. Bright Instruments...
  • Page 12: Maintenance

    4.1 CLEANING Wipe off sectioning debris etc with a paper The 8000 Sledge Microtome is constructed of towel. Alcohols (industrial methylated spirits, materials which are sensitive to hypochlorite ethanol or methanol) may be used. Xylene (bleach) solutions.
  • Page 13: Specification

    Knife guards cover full width of knife Dimensions H325 x W260 x D610mm Packing Net weight: 40kg Gross weight: 50kg Packing dimensions: H400 x W920 x D550mm Weights vary according to specification of instrument Shipping volume: 0.202m Bright Instruments...
  • Page 14: Accessories & Consumables

    • 40 x 40mm stage. • Cryo-M-Bed embedding. 8000-02 8000 Retracting base sledge microtome • Freezing to -25°C compound. (Same as 8000-01 but without standard blade) • Temperature control is • Cooling block removal tool. by current limit to Peltier • Allen Key. device.
  • Page 15 Tungsten carbide tipped knife with box, 228 x 38 x 6mm. Suitable for bone sections. NB: Needs to be resharpened at our factory. 53577 Spare fuse set for 8000-208-01. 57675 Holder for feather blades with box. 240mm, complete with pack of 50 disposable 53578 Spare fuse set for 8000-208-02.
  • Page 16: Material Safety Data Sheet

    Material Safety Data Sheets Bright Instruments...
  • Page 17: Microtome Lubrication Oil

    Physical and chemical Form: Liquid. properties: Container: Plastic Bottle (200ml). Odour: Negligible. Colour: Water White. Uses: For lubrication of 8000 microtomes. Water Solubility: Insoluble Stability and reactivity: Stable: Description: Lubricating Oil. Conditions to avoid: Strong oxidising agents Materials to avoid: No special precautions.
  • Page 18 Microtome Lubricating Oil 57425 MATERIAL SAFETY DATA SHEET 2. Mic Lubricating Oil 57425 Product Information (Cont.) Toxicoloigical Information Skin: Prolonged contact may cause defatting of the ski resulting (Health information): in cracking and soreness. Respiratory: Low volatility make inhalation unlikely at ambient temperatures.
  • Page 19: References & Drawings

    References & Drawings Bright Instruments...
  • Page 20 12. Knife Block Clamp Lever 19. Sledge Slide 5. Thickness Knob 13. Side Pillar L/H 20. Orientating Head 6. Slide Block 14. Anti-lift Plate 21. 0/11 Clamp Lever 7. Orientating Head Vice 15. Bump Stop 22. Rubber Feet 8. Knife Guard Knob Bright Instruments...
  • Page 21 5. Link Arm 12. Operating Handle 19. Slide Adjustment Screw 6. Main Arm 13. Rewind Knob 20. Slide Block L/H 7. Ball Joint 14. Rewind Handle 21. Front Anti-Lift Plate 1. REMOVE ALL BURRS, SHARP EDGES AND CORNERS. Bright Instruments...
  • Page 22 2. Knife Clamp Lever 3. Knife Clamp Screw 4. Knife Block Clamp Lever 5. Knife Block Clamp Screw 6. Knife Block T-Nut 8. Knife Guard Rod Knife Guard Spacer 10. Knife Guard Locking Knob 11. CSK Screw 12. Knife Guard Bright Instruments...
  • Page 23 2 .5º (two and a half degree) blade clearance. (This is similar to 5040 setting) Sketch shows blade angle set to 0º on the Scale. Horizontal 15º Blade Holder Angle Standard Feather Blade Angle is 35º Bright Instruments...
  • Page 24 Agent / Distributor info: Instruments 1-3 Sovereign Park, Laporte Way, Luton, Beds, LU4 8EL United Kingdom +44 (0) 808 168 9697 brightinstruments.com Member of:...

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