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Related Manuals for Bright Instruments 2212 Starlet
Summary of Contents for Bright Instruments 2212 Starlet
- Page 1 2212 Starlet Bench-Top Portable Cryostat Manual Instruments Instruments...
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Page 2: Table Of Contents
1.2.4 Positioning 2.2.2 Blade holder UV light power unit (when supplied) Electrical Connections 2.2.3 Blade angle Decontamination procedures 1.3.1 Settling 2.2.4 Coarse advance control 1.3.2 Electrical requirements 2.2.5 Section thickness control WARRANTY 1.3.4 Switching on 2.2.6 Object holder Bright Instruments... - Page 3 Figure 16. Pawl Adjustment 6.7.4 Sections curl after lifting 5.6.2 Virkon decontamination Rotary Handwheel Diagram anti-roll plate DWG: 136026-01 6.8 Sections fail to flatten and pass down blade 6.9 Cutting Temperatures 6.9.1 Fresh, unfixed tissue 6.9.2 Fixed tissue SPECIFICATIONS Bright Instruments...
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Page 4: Safety Warnings
SAFETY WARNING Low temperatures are present in this equipment. Extreme care should be taken. DO NOT let bare skin come into contact with metal surfaces. Bright Instruments... -
Page 5: Knives / Blades
SAFETY WARNING Extremely sharp knives/blades. Use knife/blade guards at all times. Use correct tools for removal and insertion of knives/blades. DO NOT leave knives/blades laying around. Place knives/blades not in use, into box/wallet provided. Bright Instruments... -
Page 6: Safety Information
Your attention is therefore drawn to the of the dangers and observe the following following precautions: warnings: a. Do not leave the microtome unattended with an exposed knife in position. Remove the knife, or cover it with the guards provided. Bright Instruments... -
Page 7: Operation
Open the window and place a beaker of ensuring that users are aware of information. containing 10ml of ammonia SG.880 in the this situation. chamber. Close the window. e. Leave for one hour. The cryostat is now decontaminated.essential that it be decontaminated. Bright Instruments... - Page 8 For further information regarding alternative decontamination procedures please refer to ‘Safe Working and the Prevention of Infection in Clinical Laboratories’ , ISBN 0 11 885446 1. Bright Instruments...
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Page 9: Warranty
Seller’s liability under this clause shall be in lieu of any warranty or condition implied by law as to the quality or fitness for any particular purpose of such goods. Bright Instruments... -
Page 10: Introduction
DESPATCH) and Bright Instrument Company Limited immediately. The microtome is supplied already installed in the chamber, carefully remove the packing from around the microtome and the anti-roll Bright Instruments... -
Page 11: Insulating Plug
(Section 2.1.3) switching on. We recommend the instrument is left standing for at least eight hours, and preferably overnight, before switching on. Bright Instruments... -
Page 12: Operating Instructions
Set button, then press Up or Down buttons until desired temperature is set. After 5 seconds the actual chamber temperature will show. QUICK FREEZER TEMPERATURE DEFROST CHAMBER TEMPERATURE To review set temperature at any time press Starlet 2212 and release Set button. Instruments Bright Instruments... -
Page 13: Microtome Controls
If the blade has by scraping it with a wooden or plastic been used only at one end, it may be reversed scraper or wiping it with a cloth dampened and refitted as described above. with alcohol. Bright Instruments... -
Page 14: Section Thickness Control
IMPORTANT: Always push the operating ANGULAR lever/hand wheel down slightly before changing thickness setting. DO NOT SCALE ! Bright Instruments IF IN DOUBT - ASK! Object Holder (34) 2.2.6 OBJECT HOLDER Cryo-M-Bed before inserting in the carrier (head)(12) and First apply a coating of embedding compound... -
Page 15: Anti-Roll Assembly
To adjust the height of the ARP turn screw Figure 12a. Figure 12b. (84) Upward adjustment can be made with the ARP against the blade; downward adjustment must Render be made with it away from the blade. If the Anti Roll Plate Render Magnaplate Anti-Roll Plate Bright Instruments... -
Page 16: Cutting & Collecting Sections
16µm. d. After cutting an acceptable section, lock h. Clean the blade surface using the bristle the specimen at the bottom by using the hand brush with an upward motion. wheel lock, or by pushing the lever back. Bright Instruments... -
Page 17: General Maintenance
0ºC. Bright Instruments... -
Page 18: Total Defrosting
See notes on Decontamination (section 3.6). minute defrosts. d. Do not extend the defrost cycle time beyond thirty minutes, as this could cause the entire chamber to warm up above 0ºC. Bright Instruments... -
Page 19: Service & Repairs
Determine which fuse is (48/72) to correct the gap. blown and replace it with one of exactly the f. Set pawl in the rewind position. same type and rating. Bright Instruments... -
Page 20: Decontamination
“Code microbiological and clinical departments. is fitted. of Practise for the Prevention of Infection in Clinical Laboratories and Post-mortem Rooms”, a. Defrost the cryostat completely. ISBN 0 11 320464 7 b. Remove the microtome (Section 3.3.3) Bright Instruments... - Page 21 Carry out a similar procedure on the microtome. g. Ensure microtome and chamber are dry. h. Apply low temperature grease sparingly to screw threads and re-install microtome in the chamber. i. The cryostat is now ready to be switched back on. Bright Instruments...
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Page 22: Frozen Sectioning
Specimen loose on holder? Has the specimen dehydrated? Freezing technique too slow? Clamping screw too loose? Cutting temperature too warm? Cutting temperatures too warm / cold? Anti-roll plate adjusted too high? Tissue expansion due to block warming up? Bright Instruments... -
Page 23: Uneven Thickness Across Section
6.7 SECTIONS CURLING 6.9 CUTTING TEMPERATURES 6.7.1 SECTIONS CURL OVER ROLL PLATE Standard textbooks on histological technique give table of recommended cutting Anti-roll plate too low? temperatures for different tissues. However, in most cases the following guidelines will prove adequate. Bright Instruments... -
Page 24: Specifications
2-12μm in 2μm increments Instruments 4-24μm optional Dimensions H315 x W465mm(min)/ 546mm(max) x D740mm Packing Net weight: 40kg Gross weight: 48kg Packing dimensions: H400 x W920 x D550mm Shipping volume: 0.20m3 Weights vary according to specification of instrument Bright Instruments... -
Page 25: Accessories & Consumables
2212 Starlet Order List 2021 PRODUCT ACCESSORIES Code Description Code Description 2212-021 Starlet 2212 Cryostat 50894-3 Cryo block embedding mould, 22mm diameter. for 22/240V AC, 50/60Hz 50894-2 Cryo block embedding mould, 16mm diameter. Complete with: Standard accessories include: 50894-1 Cryo block embedding mould, 10mm diameter. -
Page 26: Material Safety Data Sheets
Material Safety Data Sheets Bright Instruments... -
Page 27: Cryo-M-Bed 53581
Goggles should be worn. Instruments OES: Not assigned (long term, 8 hour TWA). Office: Telephone: +44 (0) 808 168 9697 Web: brightinstruments.com Bright Instruments LTD Burnett House, Lakeview Court Email: sales@brightinstruments.com Member: Instruments Ermnie Business Park, Huntingdon Cambridgeshire, PE29 6UA... -
Page 28: Cryospray 57713
An environmental hazard cannot be excluded in the event Eff ects: of unprofessional handling or disposal. Instruments Office: Telephone: +44 (0) 808 168 9697 Web: brightinstruments.com Bright Instruments LTD Burnett House, Lakeview Court Email: sales@brightinstruments.com Member: Instruments Ermnie Business Park, Huntingdon Cambridgeshire, PE29 6UA... - Page 29 Advice on common storage: Do not store together with Oxidising agents. Further information In the event of fi re, cool tanks with water spray. Instruments Bright Instruments LTD Office: Telephone: +44 (0) 808 168 9697 Web: brightinstruments.com Burnett House, Lakeview Court Email: sales@brightinstruments.com...
- Page 30 Eye protection: Goggles. Skin and body protection: Wear suitable protective equipment. Protective footwear. Instruments Office: Telephone: +44 (0) 808 168 9697 Web: brightinstruments.com Bright Instruments LTD Burnett House, Lakeview Court Email: sales@brightinstruments.com Member: Instruments Ermnie Business Park, Huntingdon Cambridgeshire, PE29 6UA...
- Page 31 MSDS are available for professional users on request. Instruments Office: Telephone: +44 (0) 808 168 9697 Web: brightinstruments.com Bright Instruments LTD Burnett House, Lakeview Court Email: sales@brightinstruments.com Member: Instruments Ermnie Business Park, Huntingdon Cambridgeshire, PE29 6UA...
- Page 32 Not in compliance with Ems number F-C,S-V published by ERMA New Zealand. inventory. Marine pollutant Instruments Office: Telephone: +44 (0) 808 168 9697 Web: brightinstruments.com Bright Instruments LTD Burnett House, Lakeview Court Email: sales@brightinstruments.com Member: Instruments Ermnie Business Park, Huntingdon Cambridgeshire, PE29 6UA...
- Page 33 An environmental hazard cannot be excluded in the event of unprofessional handling or disposal. Instruments Office: Telephone: +44 (0) 808 168 9697 Web: brightinstruments.com Bright Instruments LTD Burnett House, Lakeview Court Email: sales@brightinstruments.com Member: Instruments Ermnie Business Park, Huntingdon...
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Page 34: Low Temperature Oil 57491
Will not evaporate or dissolve Air: DO NOT allow to enter drainage systems, rivers or waterways. Instruments Office: Telephone: +44 (0) 808 168 9697 Web: brightinstruments.com Bright Instruments LTD Burnett House, Lakeview Court Email: sales@brightinstruments.com Member: Instruments Ermnie Business Park, Huntingdon Cambridgeshire, PE29 6UA... - Page 35 Regulatory information: This product is a preparation and is NOT classifi ed according to EEC Guideline. Instruments Office: Telephone: +44 (0) 808 168 9697 Web: brightinstruments.com Bright Instruments LTD Burnett House, Lakeview Court Email: sales@brightinstruments.com Member: Instruments Ermnie Business Park, Huntingdon...
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Page 36: References & Drawings
References & Drawings Bright Instruments... - Page 37 Figure A. Quick Freezer Bar QF Bar Lock Pin Object Holder Line drawing or render QF Bar Bright Instruments...
- Page 38 Figure 1. Blade Holder 1. Anti-roll Plate Height Adjuster 2. Magnets SUB - ASSEMBLY 3. Anti-roll Plate 4. Blade Guard shown open Magnacut blade not shown for clarity MAGNACUT ASSEMBLY Bright Instruments...
- Page 39 Adjustment DO NOT SCALE ! 24. Knife Angle Adjuster 24. Knife Angle Adjuster IF IN DOUBT - ASK! 25. Lock Screw 25. Lock Screw 39. Blade Holder Clamp Knob 39. Blade Holder Clamp Knob STARLET MICROTME FIXINGS REMOVED Bright Instruments...
- Page 40 Figure 4. Coarse Advance Figure 5. Section CALE ! Control Thickness Control BT - ASK! 14. Ratchet Wheel 28. Micron Indexing Dial 17. Pawl 39. Blade Holder Clamp Knob STARLET MICROTME Bright Instruments...
- Page 41 Figure 11. Trimming DO NOT SCALE ! IF IN DOUBT - ASK! 17. Pawl 12. Carrier Head 200. Rotary Main Shaft 14. Ratchet Wheel 203. Coupling Joint 17. Pawl 208. Main Shaft Coupler 20. Clamp Screw HAFT ASSEMBLY STARLET MICROTME Bright Instruments...
- Page 42 Figure 16. Pawl Adjustment 16. Spacer 73. Pawl Spring Backing Plate 13. Pawl Arm 17. Pawl 17. Pawl 97. Screw 14. Ratchet Wheel 18. Pivot Lock Screw 19. Pawl Spring 107. Roll Pin Clearance Gap: 2mm + 0.2 - 0.0 Bright Instruments...
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Page 43: Rotary Handwheel Diagram
(Refer to Fig.11) handwheel version and replace the following Drive Shaft Coupler part numbers in Fig 3, 6, 29, 30, 31, 32, 33, 39, Drive Mechanisim 41, 47, 74, 91, 92 and 95. Bright Instruments DO NOT SCALE! IF IN DOUBT ASK! -
Page 44: Dwg: 136026-01
STARLET 230V DWG: 136026-01 Description Wiring Connections DEF HTR Fuse 1AT WIRE IDENTIFICATION +1.5mm -1.0mm ELSE 0.5mm Filter Added CN1895 Black Blue Grey Pink Violet Yellow Convert to Visio Brown Green Orange White Interim Wire Colour Change CN2478 Bright Instruments... - Page 45 Instruments Instruments Instruments Instruments Agent / Distributor info: Burnett House Lakeview Court Ermnie Business Park Huntingdon Cambridgeshire Instruments PE29 6UA +44 (0) 808 168 9697 brightinstruments.com Instruments Member of:...