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Summary of Contents for CytoSMART Lux2
- Page 1 Lux2 Your best friend in the lab INSTRUCTION MANUAL...
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Page 2: Table Of Contents
INDEX Safety Information 1. Introduction Technology to Make Your Work Easier How Easy the CytoSMART™ Lux2 Works Ideal Circumstances for Cell Cultures System Overview 2. Getting Started 3. Setting Up the System Cable Placement Securing the Cable Status Indicator Vessel Placement Starting the Software 4. - Page 3 Please connect the charger Please connect to the internet Server offline, online functionality unavailable Warning! Prolonged live mode can cause increased temperature No Project Email Received Video Is Shaking 8. Technical Specification 9. Warranty 10. Cytosmart Care - Ordering Information Contact Information...
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Page 4: Safety Information
Safety Information To avoid damage or malfunction of the CytoSMART™ Lux2: • Be sure to set this device on a firm, stable and horizontal surface. The device may break or cause injury if it falls • Shut down the device immediately if it produces smoke, a strange odor or unusual noise. -
Page 5: Introduction
CO -incubator. The device is linked to a Windows tablet or computer. The Lux2 is delivered with a Windows tablet, so no additional hardware is required. Via the tablet, you can customize the imaging settings and get started. The images are then transferred to the CytoSMART™... -
Page 6: Ideal Circumstances For Cell Cultures
Ideal Circumstances for Cell Cultures With the CytoSMART™ Lux2, cells can remain in their defined culture conditions inside the incubator while monitoring them online. There is no need to disturb the ideal circumstances of your experiment. The system also provides automatic email alerts regarding the cell culture, e.g. -
Page 7: Getting Started
CytoSMART Lux2 Live Cell Imaging System Instruction Manual 2. Getting Started The content of the CytoSMART™ Lux2 package is shown below (fig. 2): CytoSMART Lux2 CytoSMART Lux2 Instruction Manual CytoSMART Lux2 Quick Starter Guide Cable clips License key Manual and Quick Start Guide CytoSMART™... -
Page 8: Setting Up The System
3. Setting up the system It is really easy to set up the CytoSMART™ Lux2 system. All you need are these items: • An incubator • The CytoSMART™ Lux2 device • Cable clips • A Windows tablet or PC •... -
Page 9: Securing The Cable
USB cable connection near the tablet. For more details, please check the quick start guide of the tablet. Status Indicator To check if the device is active, open the incubator to see if the CytoSMART™ Lux2 is illuminated: •... -
Page 10: Setup An Experiment
This is it, the real magic begins here. With the CytoSMART™ Lux2 properly placed inside the incubator and the software active, you can begin with the imaging of an experiment. Place a culture vessel on the Lux2 to begin. An image of the cells will be visible on the live screen (fig. 5). -
Page 11: Registration Of The Device
‘New registration’ (6). Fig. 6. Registration menu. To register the CytoSMART™ Lux2, enter a name (7) for the device, e.g. company name or research group. Other CytoSMART products can be added to this name. Next, create a personal account using an email address (8) and a personal password (9). -
Page 12: Account Setup
Account Setup It does not matter whether you want to include one or multiple experiments in one project. The CytoSMART™ Lux2 can do it all. An experiment is one imaging session of a cell culture experiment. Select “Start Experiment” (4) in the live screen (fig. 5), to start a new experiment simply login. -
Page 13: Start A Project
CytoSMART Lux2 Live Cell Imaging System Instruction Manual Start a Project If you are the creator of a new project, you will be assigned as a project owner or an admin (16) and this cannot be changed (fig. 10). A project can hold multiple experiments. -
Page 14: Start An Experiment
When the imaging has started, the system will go in sleep mode. For more details regarding the sleep mode, please check the quick start guide of the tablet. Fig. 11. Experiment menu. CytoSMART Lux2 Instruction Manual... -
Page 15: Ending An Experiment
A two-year subscription to the CytoSMART™ Cloud is included with the CytoSMART™ Lux2. After starting an experiment, the captured images will be stored in the cloud. To allow for this, make sure the system has an active internet connection. All time logs in the cloud are set to GMT. -
Page 16: Project Overview
Fig. 14. Login menu. Project Overview After logging in to the CytoSMART™ Cloud, you will get access to an overview of all projects (fig. 15). Navigating the portal is pretty simple. If you have multiple projects in an account, use the ‘Search Projects’... -
Page 17: Experiment Overview
CytoSMART Lux2 Live Cell Imaging System Instruction Manual Experiment Overview After selecting a project (3), you will get access to an overview of all experiments assigned to that project (fig. 16). In the top, you can see an overview of the project data (6). -
Page 18: Experiment Results
After you select a specific experiment, the data collected during that experiment will be presented in the CytoSMART™ experiment page (fig. 17). In the next few pages, you will find an overview of the panes and buttons in this Experiment Page. -
Page 19: Time-Lapse Movie Pane
CytoSMART Lux2 Live Cell Imaging System Instruction Manual Time-Lapse Movie Pane This pane shows you a time-lapse movie of the cell culture (fig. 18). You can control the playback of the movie by the buttons below the image, these include play, pause, forward one picture, backward one picture. -
Page 20: Notes Pane
Furthermore, you can delete or add these notifications at any time throughout the duration of the experiment. When a limit is reached, the project owner receives an email notification. Fig. 21. Notification pane. CytoSMART Lux2 Instruction Manual... -
Page 21: Logbook Pane
CytoSMART Lux2 Live Cell Imaging System Instruction Manual Logbook Pane The logbook pane shows a log of all the changes made to the experiment (fig. 22). Fig. 22. Logbook pane. Cell Coverage Graph In this graph, the cell coverage - in percentage of surface area - is plotted against the duration of the experiment (fig. -
Page 22: Offline Experiment
6. Offline Experiment Internet access is not required for you to experiment. With the CytoSMART™ Lux2 it is possible to perform an offline experiment when there is no internet access. However, we recommend to only use this for short-term experiments. Furthermore, access to the results will be limited to the system directly connected to the CytoSMART™... - Page 23 CytoSMART Lux2 Live Cell Imaging System Instruction Manual After selecting ‘Start Experiment’, the offline experiment menu will appear (Fig. 26). Provide an experiment name here (2) to proceed. Next, select the desired imaging frequency (3). A drop down menu will appear with different intervals to choose from.
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Page 24: Ending An Offline Experiment
Fig. 27. Ending an offline experiment. Accessing Experiment Results To access the obtained results (Fig. 28), select ‘Menu’ and then ‘Browse’ (6) from the live screen. To minimize the CytoSMART software, select ‘Menu’ and then ‘Minimize’ (7). Fig. 28. Accessing results. -
Page 25: Cleaning And Troubleshooting
A scratched or broken cover glass can be repaired but this is not included within the product’s warranty. Important: Do not use acetone to clean the CytoSMART™ Lux2. Also, do not autoclave the CytoSMART™ Lux2, this can result in damage to the electronics. -
Page 26: Please Connect The Device
Please connect the charger When the tablet is not connected to a functioning power outlet, connectivity problems may occur with the CytoSMART™ Lux2. To resolve this, make sure the tablet is connected to a functional power outlet using the provided charger. -
Page 27: Server Offline, Online Functionality Unavailable
If this error remains, please contact our support, via support@cytosmart.com. Warning! Prolonged live mode can cause increased temperature When the CytoSMART™ Lux2 has been active for 3 minutes continuously, due to activity in the live screen, this warning will appear. -
Page 28: Technical Specification
133 x 90 x 100 mm (LxWxH) or 5,2” x 3,5” x 3.9” (LxWxH) Weight 0.5 kg or 1.1 lbs. Data Storage Unlimited storage in the CytoSMART™ Cloud with the subscription. Operating Environment 5-40 ° C, 20-95% humidity Minimal System Requirements Windows 7 or higher 1.44 GHz CPU... -
Page 29: Warranty
9. Warranty The CytoSMART Lux2 is covered by warranty - if the device is properly used and installed - for a period of one year (1 year) from the date of purchase. If any defects occur during this warranty period, CytoSMART will repair or replace the defective parts at its discretion without charge. -
Page 30: Cytosmart Care - Ordering Information
10. Ordering Information Would you like to purchase extended warranty for the CytoSMART™ Lux2? These products are available separately from CytoSMART for the duration of 1 year, 2 years or 5 years. Cat. No. Description Contents HCA-1003 CytoSMART Care - Lux2, option 1... - Page 31 CytoSMART Lux2 Live Cell Imaging System Instruction Manual NOTES...
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Page 32: Contact Information
The buyer assumes all risks of use and/or handling. Any user must make his own determination and satisfy himself that the products supplied by CytoSMART™ Technologies or its affiliates and the information and recommendations given by CytoSMART™ Technologies or its affiliates are (i) suitable for intended process or purpose, (ii) in compliance with environmental, health and safety regulations, and (iii) will not infringe any third party’s...
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