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Himedia Insta NX Product Information

Fungal dna purification kit

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U n z i p p i n g
G e n e s
MBIN008
Kit Contents
Product Code
DS0401
Fungal DNA Extraction Cartridges
DS0337
1 ml tip set
DS0016
Lysis Buffer (PL)
DS0017
Precipitation Buffer (PS)
DS0003
RNase A Solution (20 mg/ml)
DS0040
Elution Buffer (ET) [10 mM Tris-Cl, pH 8.5]
DBCA04
HiBead Tubes
DSCA02
HiShredder (in DBCA016 Collection Tube)
PW1139
Collection Tubes, Polypropylene (2.0 ml)
DBCA021
Columns for Insta NX
Introduction
This kit provides a fast and easy method to purify total DNA from fungal samples for
downstream applications such as PCR, Southern blotting technique etc. The DNA purification
procedure using the column comprises of three steps viz. adsorption of DNA to the Super- S
membrane, removal of residual contaminants and elution of pure genomic DNA. The Super- S
columns have a high binding capacity and high quality genomic DNA is obtained from various
species.
Insta NX® Fungal DNA Purification Kit
This kit simplifies isolation of DNA from various fungal samples with vacuum based innovative
Super- S column technology. The Super- S columns contains specially developed membranes for
optimal binding of genomic DNA. After the initial binding of DNA, impurities like proteins,
polysaccharides, low molecular weight metabolites and salts are removed by short washing
steps. High quality DNA is finally eluted in the Elution Buffer provided with the kit.
The salient features of this kit are as follows:
a. Cross- Contamination free
b. 12 samples can be processed at a time.
c. Prefilled reagent cartridge
d. High purity, high yield, as good as manual.
Elution
The yield of genomic DNA depends on the sample type and the number of cells in the sample.
An elution with 100 µl of Elution Buffer (ET) will provide sufficient DNA to carry out multiple
amplification reactions. Elution with volume less than 100 µl will increase the final DNA
concentration, but will reduce the overall DNA yield. The eluted DNA ranges in size upto 20-30
kb, and is suitable for direct use in PCR, restriction digestion and Southern blotting applications.
WHO
15
GMP
CERTIFIED
Insta NX® Fungal DNA Purification Kit
Reagents provided
1
Registered Office :
23, Vadhani Industrial Estate,LBS Marg,
Mumbai - 400 086, India.
Tel. : (022) 4017 9797 / 2500 1607
Fax : (022) 2500 2286
The information contained herein is believed to be accurate and complete. However no warranty or guarantee whatsoever is made
or is to be implied with respect to such information or with respect to any product, method or apparatus referred to herein
P r o d u c t I n f o r m a t i o n
12 Preps
12 nos.
12 nos.
6 ml
1.8 ml
300 µl
350 µl
12 nos.
12 nos.
40 nos.
12 nos.
Commercial Office
A-516, Swastik Disha Business Park,
Via Vadhani Indl. Est., LBS Marg,
Mumbai - 400 086, India
Tel: 00-91-22-6147 1919
Fax: 6147 1920, 2500 5764
Email : info@himedialabs.com
Web : www.himedialabs.com

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Summary of Contents for Himedia Insta NX

  • Page 1 P r o d u c t I n f o r m a t i o n U n z i p p i n g G e n e s MBIN008 Insta NX® Fungal DNA Purification Kit Kit Contents Product Code Reagents provided...
  • Page 2 1.6-1.9. Purity is determined by calculating the ratio of absorbance at 260 nm to absorbance at 280 nm. DNA purified by Insta NX® Fungal DNA Purification Kit is free of protein and other contaminants that can inhibit PCR or other enzymatic reactions.
  • Page 3 Procedure (For Non- Barcode Mode) 1. Turn on Insta NX® instrument. Allow the instrument to initialize. 2. Once the instrument is ready, select “Login” on the instrument screen. Fig. 1 3. Enter the Password to login. Fig. 2 4. On the Home screen, select “ Start Purification”...
  • Page 4 Select Elution volume: 50/ 100/150 or 200µl b. Select Kit Name: Select the type of extraction procedure to be performed. MBIN008 Insta NX® Fungal DNA Fig. 5 7. Lift open the front door and take out the 12-in-1 rack for preparation.
  • Page 5 9. Place the columns (DBCA021) on the cartridge as shown below: Fig. 7 10. Place 1ml tip (DS0337) set onto the 12-in-1 rack. Fig. 8 11. Open the metal lid and place the Collection tube, Polypropylene (PW1139) into 12-in-1 rack (as shown in figure below) and close the metal lid.
  • Page 6 Transfer the flow through without disturbing the pellet to a new Collection tube (provided)  Proceed with step 14 for DNA purification in Insta NX® Machine as follows. 14. Place the Collection tube containing sample onto the 12-in-1 rack. Fig. 11...
  • Page 7 15. Close the front door. Click Start Run. MBIN008 Insta NX® Fungal DNA Purification Fig. 12 16. Following screen will appear while the run is going on. Fig. 13 17. After the run is completed, the screen will look like the below figure: Run complete Fig.
  • Page 8: Troubleshooting Guide

    Fig. a: Insta Q 96-PLUS Fig. b: Wee Machine Representative image of PCR set up in HiMedia’s PCR Machines Storage of the eluate with purified DNA: The eluate contains pure DNA. For short-term storage (24-48 hrs.) of the DNA, 2-8°C is recommended. For long-term storage, -20°C or lower temperature (-80°C) is recommended.
  • Page 9 Technical Assistance At HiMedia, we pride ourselves on the quality and availability of our technical support. For any kind of technical assistance, mail to mb@himedialabs.com.

This manual is also suitable for:

Mbin008