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U n z i p p i n g
G e n e s
HiPer
Number of experiments that can be performed: 10
The kit is stable for 12 months from the date of manufacture
50 bp DNA Ladder, Primer mix for SNP and Molecular Biology Grade
Other kit contents can be stored at room temperature (15-25
WHO
15
GMP
CERTIFIED
®
Single Nucleotide Polymorphism
(SNP) Teaching Kit
Product Code: HTBM034
Duration of Experiment:
Protocol: 2 hours
Agarose Gel Electrophoresis: 45 minutes
Storage Instructions:
Store 2X PCR Master Mix, Normal DNA, SNP DNA,
Store 6X Gel Loading Buffer at 2-8
Registered Office :
23, Vadhani Industrial Estate,LBS Marg,
Mumbai - 400 086, India.
Tel. : (022) 4017 9797 / 2500 1607
Fax : (022) 2500 2286
The information contained herein is believed to be accurate and complete. However no warranty or guarantee whatsoever is made
or is to be implied with respect to such information or with respect to any product, method or apparatus referred to herein
P r o d u c t I n f o r m a t i o n
Water at -20
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C
1
Commercial Office
A-516, Swastik Disha Business Park,
Via Vadhani Indl. Est., LBS Marg,
Mumbai - 400 086, India
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C
o
C)
Tel: 00-91-22-6147 1919
Fax: 6147 1920, 2500 5764
Email : info@himedialabs.com
Web : www.himedialabs.com
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Related Manuals for Himedia HiGenoMB HIPERж
Summary of Contents for Himedia HiGenoMB HIPERж
- Page 1 P r o d u c t I n f o r m a t i o n U n z i p p i n g G e n e s ® HiPer Single Nucleotide Polymorphism (SNP) Teaching Kit Product Code: HTBM034 Number of experiments that can be performed: 10 Duration of Experiment:...
- Page 2 Index Sr. No. Contents Page No. Introduction Principle Kit Contents Materials Required But Not Provided Storage Important Instructions Procedure Agarose Gel Electrophoresis Observation and Result Interpretation Troubleshooting Guide...
- Page 3 Aim: To detect genetic disorder through the study of Single nucleotide polymorphism Introduction: Single nucleotide polymorphism (SNP) is a variation in the DNA sequence at a precise position among individuals which lead to genetic variation among people. For example, a SNP may replace the nucleotide cytosine (C) with the nucleotide thymine (T) in a certain stretch of DNA.
- Page 4 Kit Contents: The kit can be used to amplify a particular template DNA using PCR. Table 1: Enlists the materials provided in this kit with their quantity and recommended storage Quantity Product Code Materials Provided Storage 10 expts MBT061 2X PCR Master Mix 0.6 ml *TKC415 SNP DNA...
- Page 5 Procedure: 1) Preparation of master mix for PCR Take two PCR tubes, label them and add the following ingredients in mentioned order: Test Sample Test Sample Molecular Biology Grade Water 19 µl 19 µl 2X PCR Master mix 25 µl 25 µl SNP DNA 5 µl...
- Page 6 Agarose Gel Electrophoresis: Preparation of 1X TAE: To prepare 500 ml of 1X TAE buffer, add 10 ml of 50X TAE Buffer to 490 ml of sterile distilled water*. Mix well before use. To prepare 50 ml of 2% agarose gel, add 1 g agarose to 50ml of 1X TAE buffer Preparation of agarose gel: in a glass beaker or flask.
- Page 7 Interpretation: In this PCR reaction two targets are amplified simultaneously, one is specific for Staphylococcus genus and the other for methicillin-resistant staphylococci (mecA gene). The target specific for Staphylococcus genus amplifies in both SNP and normal DNA but the MRSA specific mecA gene is amplified only in the SNP DNA which is extracted from a MRSA strain.
- Page 8 Technical Assistance: At HiMedia we pride ourselves on the quality and availability of our technical support. For any kind of technical assistance, mail at mb@himedialabs.com 25°C Storage temperature 15°C Do not use if package is damaged HiMedia Laboratories Pvt. Limited,...
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