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Ultra-Portable Real-Time Fluorescence
Quantitative PCR Instrument
Instruction Manual
The instruction manual must be properly placed in the product box during shipment.
The user is required to keep this manual in a safe place so that it can be consulted when
needed.
All rights reserved. All information contained in this manual is subject to change without
notice.
Manufacturer:
Anitoa Biotechnology (Hangzhou) Co., Ltd.
Address: China-Australia Biomedical Industry Science and Technology Park, No.656
Binan Road, Binjiang District, Hangzhou, Zhejiang, China
Thank you for choosing our products. Please read this instruction manual carefully before use.
Maverick qPCR
Version（V2.1）
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Summary of Contents for anitoa Maverick qPCR
- Page 1 All rights reserved. All information contained in this manual is subject to change without notice. Manufacturer: Anitoa Biotechnology (Hangzhou) Co., Ltd. Address: China-Australia Biomedical Industry Science and Technology Park, No.656 Binan Road, Binjiang District, Hangzhou, Zhejiang, China Thank you for choosing our products. Please read this instruction manual carefully before use.
- Page 2 Software Description： The software is a necessary tool for the operation of the instrument and Anitoa has the right to modify its functions or design, etc., in order to improve its operational performance and reliability, without prior or subsequent notification to the customer, and Anitoa has all the intellectual property rights for the modified version.
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Page 3: Table Of Contents
Anitoa Biotechnology (Hangzhou) Co., Ltd. CONTENTS Chapter 1 Important Notes ............1 1.1 Instrument Grounding ............1 1.2 Placement of the Instrument ........... 1 1.3 Operation Note ..............2 1.4 After-Sales Service ............2 1.5 Packaging, Storage and Transportation Marking ......... 3 1.6 Instrument Identification Information ......... -
Page 4: Chapter 1 Important Notes
Anitoa Biotechnology (Hangzhou) Co., Ltd. Chapter 1 Important Notes The following basic safety measures must be observed during all phases of operation, maintenance and repair of this instrument. Failure to observe these measures or the warnings and precautions indicated in this manual will likely affect the basic protection provided by the instrument. -
Page 5: Operation Note
Anitoa Biotechnology (Hangzhou) Co., Ltd. The instrument uses semiconductor cooling and fan-assisted heat dissipation, so when placing the instrument, ensure that there are no obstacles within 15 cm of the instrument. The instrument should be placed in a place with low humidity, less dust and far from water sources (such as pools, water pipes, etc.), with good ventilation, no corrosive gases or strong... -
Page 6: Packaging, Storage And Transportation Marking
Anitoa Biotechnology (Hangzhou) Co., Ltd. 1.5 Packaging, Storage and Transportation Marking Table 1.5 Package storage and transportation identification Description Symbol Title Position This symbol is used to indicate that Place the product is a precision On the packing carefully instrument and should be handled... -
Page 7: Instrument Identification Information
Anitoa Biotechnology (Hangzhou) Co., Ltd. 1.6 Instrument Identification Information Table 1.6 Instrument identification information The location on the instrument Symbol Description where the symbol will appear Watch out for high On the equipment temperatures Production date On the equipment nameplate... -
Page 8: Fcc Statement
Anitoa Biotechnology (Hangzhou) Co., Ltd. 1.7 FCC Statement This device complies with part 15 of the FCC rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept... -
Page 9: Chapter 2 Product Overview
Anitoa Biotechnology (Hangzhou) Co., Ltd. Chapter 2 Product Overview This chapter mainly describes the usage, characteristics, specifications and performance parameters of MQ4 fluorescence quantitative PCR instrument. 2.1 Product Usage The MQ4 model is an ultra-portable qPCR instrument with a large 10" touch screen for integrated operation. -
Page 10: Specification Model Description
Anitoa Biotechnology (Hangzhou) Co., Ltd. Specification Model Description 4：Represents the number of channels of the instrument 16：Represents sample throughputs 4： Represents appearance number MQ：Represents series number 2.4 Instrument Models Table 2.4 Instrument models Model Channel Sample throughput MQ4044 4 channels... -
Page 11: Wifi And Bt
Anitoa Biotechnology (Hangzhou) Co., Ltd. Fluorescence Detection System Performance Light Source High Brightness LED Detector Ultra-Low-Light CMOS Image Sensor (CIS) Chips F1：470nm F2：523nm Excitation Wavelength F3：571nm F4：624nm F1：527nm F2：564nm Detection Wavelength F3：612nm F4：694nm 2.6 WIFI and BT The XY8788 module provides a shared antenna interface ANT_WIFI/BT for WIFI and BT with an impedance of 50Ω... - Page 12 Anitoa Biotechnology (Hangzhou) Co., Ltd. 2.6.2 WIFI performance indicators The following table lists the transmit and receive performance of XY8788 WIFI Table 24: Transmission performance of WIFI Output Power standard rate 17±2dBm 802.11b 1Mbps 802.11 11Mbps 16±2dBm 802.11g 6Mbps 15±2dBm ...
- Page 13 Anitoa Biotechnology (Hangzhou) Co., Ltd. Reference Specification serial document number numb IEEE 802.11n WLAN MAC and PHY, October 2009 + IEEE 802.11-2007 WLAN MAC and PHY PHY, June 2007 IEEE 802.11b, IEEE Std 802.11d, IEEE 802.11e, IEEE 802.11g, IEEE Std 802.11i: IEEE 802.11-2007 WLAN MAC and PHY, June 2007 2.6.3 BT overview...
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Page 14: Chapter 3 Instrument Installation
Anitoa Biotechnology (Hangzhou) Co., Ltd. Chapter 3 Instrument Installation This chapter describes the use and storage conditions of the MQ4 ultra-portable quantitative fluorescence PCR instrument, its construction, removal of the fixture, installation/uninstallation of the software, and preparation for power-up. 3.1 Environmental Conditions 1) Transportation and storage conditions of the instruments a. -
Page 15: Power Cord Connection
Anitoa Biotechnology (Hangzhou) Co., Ltd. Power Adapter USB Cable Instruction Manual Factory Inspection Report Warranty Card Certificate of Conformity 3.4 Power Cord Connection 1) Connection of adapter: the adapter supplied with the instrument should be used to connect the adapter to the instrument. - Page 16 Anitoa Biotechnology (Hangzhou) Co., Ltd. SampleTable Location Instrument Running Indicator Light Figure 3.6-2 Front of the instrument Insert the DC connector into the instrument interface, and the "click" sound means that the connector is in place Open the main switch of the instrument (boat switch), and the red indicator of the instrument...
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Page 17: Chapter 4 Software Operation Guide
"System" module: mainly contains WLAN, language, virtual keyboard, date and time, display, and application language. 4.1 Launch Software 1) Launch the screen software "anitoa" to enter the login screen, as shown in Figure 4.1-1, and click the "Login" button. Figure 4.1-1 Login Interface... -
Page 18: Experimental Setup
Anitoa Biotechnology (Hangzhou) Co., Ltd. When asked if the USB connection is allowed, click < OK>, as shown in Figure 4.1-2 Figure 4.1-2 USB Access interface 4.2 Experimental Setup 1) In the test interface, click "New Experiment" (Figure 4.2-1) to enter the experiment settings 1 Figure 4.2-1 New experiment interface... - Page 19 Anitoa Biotechnology (Hangzhou) Co., Ltd. 2) In Experiment Setup 1 (Figure 4.2-2), enter the experiment name, select the corresponding channel, select the well position, and enter the sample information, etc., and click "Next" to enter Experiment Setup 2. Figure 4.2-2 Experimental setup 1 3) In Experimental Setup 2 (Figure 4.2-3), set the reaction program (parameters such...
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Page 20: Save Template
Anitoa Biotechnology (Hangzhou) Co., Ltd. 4.3 Save Template 1) Click the "Save Template" icon in the upper right corner to save the current template. Figure 4.3-1 Program setting interface 2) The saved templates will appear in the "Test" screen for selection. Click "Import Template"... -
Page 21: Running Experiments
Anitoa Biotechnology (Hangzhou) Co., Ltd. 4.4 Running Experiments After the experiment setting is completed, click the "Start" button to start the experiment and enter the "Run" interface. Click "Force Stop" at the bottom right corner to stop the experiment. Figure 4.4-1 Running interface 4.5 Experimental analysis... - Page 22 Anitoa Biotechnology (Hangzhou) Co., Ltd. Figure 4.5-1 Data list page 2) To view the amplification curve: click on the experimental data and enter the "Result Analysis" page, as shown in Figure 4.5-2. Figure 4.5-2 Result analysis interface 3) Experimental analysis a.
- Page 23 Anitoa Biotechnology (Hangzhou) Co., Ltd. Figure 4.5-3 Experimental analysis interface d. Click the "Filter" button in the upper right corner to enter the page in Figure 4.5-4 and select the well and channel for data analysis. e. Click the "Result" button in the upper-right corner to return to the Figure 4.5-3 page.
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Page 24: Data Export
Anitoa Biotechnology (Hangzhou) Co., Ltd. interface (see Figure 4.5-4). b. Manually enter the standard concentration, plot the standard curve, and calculate the concentration of the unknown sample based on the Ct value. Figure 4.5-4 Standard curve interface 4.6 Data Export Click the "Export"... - Page 25 Anitoa Biotechnology (Hangzhou) Co., Ltd. Figure 4.6-1 Data Export Select one of the export formats, such as "PDF" to enter the PDF preview interface. Figure 4.6-2 PDF preview page Click on the upper right corner of the "OK" button, the pop-up "saved successfully, whether to print PDF documents"...
- Page 26 Anitoa Biotechnology (Hangzhou) Co., Ltd. Figure 4.6-3 Whether to print the confirmation page Click the "OK" button to enter the Figure 4.6-4 page Figure 4.6-4 Save as PDF file page Click the upper left corner of the "drop-down" button, "Save as PDF" or "Add Printer"...
- Page 27 Anitoa Biotechnology (Hangzhou) Co., Ltd. Click the "Save as PDF" option to save the file in the system directory, the storage path: /sdcard/anitoa/report Figure 4.6-6 Save path System save default path： a. PDF path：/sdcard/anitoa/report b. CSV path：/sdcard/anitoa/report c. JSON path：/sdcard/anitoa/imageData JPG path：/sdcard/anitoa/imageData...
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Page 28: Chapter 5 Instrument Maintenance
Anitoa Biotechnology (Hangzhou) Co., Ltd. Chapter 5 Instrument Maintenance 5.1 Instrument Cleaning (1) Instrument surface cleaning: The surface of the instrument should be scrubbed regularly with a soft cloth with 75% alcohol, and the instrument should be wiped dry after cleaning. -
Page 29: Operation Requirements
Anitoa Biotechnology (Hangzhou) Co., Ltd. force all ongoing operations to stop. (2) After the above-mentioned failure of the heating system, the user should stop using the instrument and contact the manufacturer for repair in time. 5.5 Operation Requirements (1) During the use of the instrument, the operator may be exposed to residual substances harmful to organisms or infectious substances, and the operator needs to be trained and have relevant qualifications. -
Page 30: Chapter 6 Faq
Anitoa Biotechnology (Hangzhou) Co., Ltd. Chapter 6 FAQ Failure Cause Analysis Processing Phenomenon Must replace screen Screen motherboard motherboard, please contact with damage the supplier or manufacturer If the screen lock function incorrectly Close the lock screen, and enter The screen shows a... - Page 31 Anitoa Biotechnology (Hangzhou) Co., Ltd. data after experimental parameters and sample parameters instrument parameters are set correctly, and set the operation fluorescent markers Report upload and Please check if the instrument WIFI print function WIFI setting error setting is correct, please connect...
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