DMT 820MO User Manual
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DMT 820MO User Manual

Organ bath system
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ORGAN BATH SYSTEM – 820MO
USER MANUAL, VOL. 1.2

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  • Page 1 ORGAN BATH SYSTEM – 820MO USER MANUAL, VOL. 1.2...
  • Page 2 Since then, changes may have been made to the software and hardware it describes. New information may be supplied separately. This documentation is provided with the DMT Organ Bath System – Model 820MO Document Number: 820MO – UG2.2A All rights reserved. No part of this manual may be reproduced or transmitted in any form or by any means without the written permission of Danish Myo Technology A/S.

  • Page 3: Table Of Contents

    1.1 Interface 1.2 Interface Rear Panel 1.3 Organ Bath Chamber - 820MO CHAPTER 2 - SETTING UP 2.1 Setting up the Organ Bath System - 820MO 2.2 Setting up step-by-step CHAPTER 3 - THE INTERFACE MENUS 3.1 Interface Menus - Navigation 3.2 Interface Menus –...

  • Page 4: Safety

    SAFETY The Organ Bath System - 820MO has been designed for teaching and research only. It is not intended for clinical or critical life-care use and should never be used for these purposes. The 820MO also should not be used for the prevention, diagnosis, curing, treatment, or alleviation of disease, injury, or handicap.

  • Page 5: Unpacking The Myograph System

    UNPACKING THE ORGAN BATH SYSTEM Take a few minutes to carefully inspect your new Organ Bath System - 820MO for damage that may have occurred during handling and shipping. If you suspect any damage, please contact DMT immediately, and the matter will be pursued as soon as possible.

  • Page 6: Ec Declaration Of Conformity

    EC DECLARATION OF CONFORMITY DMT A/S Certify and declare that the following apparatus: ORGAN BATH SYSTEM - MODEL 820MO Restrictive use: Only for laboratory use. Manufactured by: DMT A/S Rho 14 8382 Hinnerup Denmark Conforms with the essential requirements of the EMC Directive 2004/108/EC.

  • Page 7: Chapter 1 - System Overview

    CHAPTER 1 - SYSTEM OVERVIEW 1.1 INTERFACE Myo-Interface display Heat indicator Power indicator Valve buttons Figure 1.1 Interface front panel...

  • Page 8: Interface Rear Panel

    1.2 INTERFACE REAR PANEL Gas input pH meter connection Temperature probe Gas regulator Transducer connections connection (needle valve) USB output (digital) ON/OFF switch Power connector 4 Recorder outputs (analogue) Vacuum input Grounding connected to Data Acquisition Figure 1.2 Interface Rear Panel...

  • Page 9: Organ Bath Chamber - 820Mo

    1.3 ORGAN BATH CHAMBER - 820MO Connection to Organ Bath Interface Micrometer positioner Figure 1.3 820MO Organ bath chamber unit and 820MO-XL Chamber Unit Organ bath clamp support Force transducer pin connected to micrometer Organ bath clamp support connected to force transducer...

  • Page 10: Chapter 2 - Setting Up

    CHAPTER 2 - SETTING UP 2.1 SETTING UP THE MUSCLE STRIP MYOGRAPH SYSTEM - 820MS Figure 2.1 The complete Organ Bath System – 820MO...

  • Page 11: Setting Up Step-By-Step

    Figure 2.1. Interface – PC Connection: Data acquisition is possible either by connecting the 820MO Interface directly to a PC with LabChart Pro and the DMT Device enabler installed or through the BNC connectors, and BNC cables from the 820MO Interface e.g., to the Powerlab box and the Powerlab box is then connected using a USB cable to the computer with...
  • Page 12 Figure 2.4B - Chamber cover with built-in electrodes (optional item) Initial Force Transducer Status: Prior to the shipment of the Organ Bath 820MO System, it has gone through two days of continuous testing, including a final force transducer calibration. However, DMT recommends that a new force transducer calibration is performed before using the organ bath system for the first time.

  • Page 13: Chapter 3 - The Interface Menus

    CHAPTER 3 - THE INTERFACE MENUS Chapter 3 is a complete manual for the 820MO Interface menus. This chapter contains a detailed description of how to navigate the touch-screen menus and how to use the special features of the 820MO myograph.
  • Page 14 3.2 INTERFACE MENUS POWER-UP SCREEN After turning on the 820MO Interface, an “Introduction” screen appears. The system is auto-calibrating the A/D converters while this screen is displayed. After a few seconds, the “ACTUAL FORCE” display will appear. At any given time, if the force applied on any channel is out of range, the force reading for the overloaded channel will turn yellow as a warning.
  • Page 15 Three menus are accessible from the default “Actual Force” screen or display. These menus are Zero, Heat, and Settings. ZERO MENU This menu is used to zero the output of the transducers. When using a data acquisition program like LabChart by AD Instruments ®, using this feature will reset the baseline of the chart traces without affecting the calibrations or physically changing any pre-load tensions placed on the mounted vessels.

  • Page 16: Interface Menus - Calibrated Mode

    SETTINGS MENU The “Settings Menu” contains several sub-menus that can be accessed to change functional aspects of the Interface. These sub- menus include: Force Calibration II. pH Calibration III. Force REC. OUTPUT IV. Measurement Range V. Interface Settings Force Calibration Entering the FORCE CALIBRATION sub-menu begins the transducer calibration procedure.
  • Page 17 Step 1 involves setting up the chamber for calibration. Make sure the chamber contains the pins or clamps, depending on the type of tissue being studied. Fill the chamber with double-distilled water for the volume to be used experimentally. Press “NEXT STEP”. Step 2 involves setting up the calibration kit appropriately for the actual weight calibration.
  • Page 18 “NEXT STEP”. Calibrate the other chambers in the same manner. 2. pH Calibration Menu (Optional) The pH Module in the 820MO is locked by a code. If the lock screen appears, a code is needed from DMT to unlock the pH module.
  • Page 19 LOGIN CODE TO pH The 820MO system has a build-in pH meter and a pH-meter Call DMT A/S to get access code. electrode plug-in port marked PH on the backside of the 820M interface. The pH electrode can be ordered at DMT by contacting your sales System ID number.
  • Page 20 Step 4 - The calibration is now finished. The values in the two bottom lines are the actual pH and temperature reading. 3. Force Rec. output (analog output only) The FORCE RECORDING OUTPUT, or FORCE REC. OUT,sub- menu determines the upper limit for force sent through the BNC output connectors to e.g.
  • Page 21 Rec Output values at the same time. Pressing “ENTER” will store the numbers in memory for future experiments. NOTE: FOR USERS USING THE ANALOG BNC OUTPUTS OF THE 820MO INTERFACE FOR DATA ACQUISITION ON A POWERLAB OR SIMILAR SYSTEM: ANYTIME THE FORCE REC OUTPUT IS CHANGED, A NEW 2-POINT CALIBRATION MUST BE PERFORMED IN E.G.
  • Page 22 Rec Output values at the same time. Pressing “ENTER” will store the values in memory for future experiments. NOTE: FOR DMT DEVICE ENABLER USERS LABCHART HAS TO BE RESTARTED AFTER CHANGING THE MEASUREMENT RANGE VALUES OTHERWISE THE OLD SETTING WILL PERSIST IN LABCHART...
  • Page 23 This is referred to as a temperature offset (TEMP OFFSET ON CHAMBER). Use the thermometer connected to the backside of the 820MO Interface to check the actual temperature of the buffer in the chamber and use that information to adjust the offset temperatures.
  • Page 24 II. Valve Delay Pressing “VALVE DELAY” in the INTERFACE SETTINGS sub-menu will allow the user to modify the time duration that the vacuum valves stay open for washes. Factory default is set at 1sec, but 1sec is usually not enough time to completely empty a chamber with even as small a volume of 5ml.
  • Page 25 IV. Select mN or g The contraction force can be shown in gram or in mN on the touch screen, and for LabChart Pro and DMT device enabler users, the selected force unit will be shown automatically in LabChart as well. For users of Powerlab or similar systems, the...
  • Page 26 The color of the text will turn black when it is saved in the system. NOTE: FOR DMT DEVICE ENABLER USERS (NO POWERLAB) IT IS VERY IMPORTANT TO RESTART LABCHART PRO AFTER CHANGING THE FORCE UNITS OTHERWISE THE FORCE UNITS WILL NOT BE TRANSFERRED TO LABCHART.
  • Page 27 In this menu, the active myograph menu is marked by bold letters. To change to one of the other systems, press SELECT, and the text line will turn blue, and then press ENTER. The text line will not be in bold letters indicating that this systems menu structure is selected.

  • Page 28: Chapter 4 - The Organ Bath Chamber Unit

    CHAPTER 4 - THE ORGAN BATH CHAMBER UNIT Chapter 4 contains a complete explanation of how to calibrate and maintain the 820MO organ bath to ensure the equipment is always performing at peak performance. 4.1 CALIBRATION OF THE FORCE TRANSDUCER As a part of the general maintenance of the myograph, DMT recommends that the myograph be weight-calibrated at least once a month.
  • Page 29 depending on the temperature regulation in the room the myograph is set up in. Obviously, lower temperatures take less time, and higher temperatures take more time to reach. Make sure adequate time is allowed so that calibration can be performed at the temperature at which the experiments will be performed.

  • Page 30: Checking The Force Transducer

    AND the transducer cannot be recalibrated, then the force transducer is broken and needs to be replaced. If any other problems related to the force transducer are encountered, please contact DMT for advice or further instructions.

  • Page 31: Force Transducer Replacement

    4.3 FORCE TRANSDUCER REPLACEMENT If the force transducer breaks and needs to be replaced, follow this step-by-step replacement procedure carefully: Remove the pin/clamp from the transducer pin coming out of the transducer house. Disconnect the Organ Bath Chamber from the Interface. Turn the Organ Bath Chamber upside down and remove the transducer housing by loosening the two screws (A+B) as illustrated in figure 4.4.
  • Page 32 IMPORTANT: CALIBRATE THE NEW FORCE TRANSDUCER BEFORE PERFORMING A NEW EXPERIMENT, AS DESCRIBED IN “CHAPTER 3 - THE INTERFACE MENUS” AND “4.1 CALIBRATION OF THE FORCE TRANSDUCER”. Figure 4.5 The transducer in the transducer housing and close-up of transducer pin inside the transducer.

  • Page 33: Organ Bath Maintenance

    4.4 ORGAN BATH MAINTENANCE The Organ Bath System - 820MO is a very delicate and sophisticated piece of research equipment. DMT recommends that the following sections are read carefully and that the instructions are followed at all times. ORGAN BATH CHAMBER TUBING To prevent the tubing from becoming blocked with buffer salt deposits after an experiment, remove the chamber cover from the Organ Bath Chamber.

  • Page 34: Cleaning The System

    Figure 4.6 The areas where linear slide grease may be applied for smooth micropositioner movement 4.5 CLEANING THE ORGAN BATH DMT strongly recommends that the organ bath chambers and surrounding areas are cleaned after each experiment. At the end of each experiment, use the following procedure to clean the organ bath chambers and supports: Fill the organ bath chamber to the edge with an 8% acetic acid solution and allow it to work for a few minutes to dissolve calcium deposits and other salt build-up.
  • Page 35 solution for about 1-2 minutes. Wash the organ bath chamber and supports several times with double distilled water. If acids such as 1M HCl and 3M HNO are used to clean the chambers, make sure ALL surfaces are thoroughly dried after copious washes with double distilled water. Any residual acid will cause corrosion of the stainless steel chamber and/or mounting supports.

  • Page 36: Chapter 5 - Mounting Supports And Muscle Mounting

    CHAPTER 5 – MOUNTING SUPPORTS AND MUSCLE MOUNTING IN THE 820MO CHAMBER Each chamber contains supports with mounting clamps to facilitate the mounting of muscle strips in the organ bath chambers. These clamps have been designed to optimally “clamp” the tissue without slipping during a contraction.
  • Page 37 DAMAGE THE FORCE TRANSDUCER. Below is a short description of how to mount a tissue ring preparation on the Mounting Pins of the 820MO system. DMT deliver the 820MO system with 200µm pins allowing the user to mount ring preparation with an internal diameter from 450µm and up.
  • Page 38 9. The mounted tissue is now ready for experiments. 3.2 NORMALIZATION – OPTIMAL PRE-LOAD TENSION Artery tissue: DMT highly recommend the user to read the DMT Normalization Guide before performing any experiments on arteries. The Normalization guide can be downloaded on www.dmt.dk.
  • Page 39 The active response of a preparation is dependent on the extent of stretch, which makes it important to set the preparation to an internal circumference or pre-load tension giving maximal response of a given tissue mounted in the 820MO chambers. The aim of the normalization procedure is to stretch the mounted muscle segment to an optimal pre-load tension giving maximum response by muscle contraction.

  • Page 40: Appendix 1 - System Specifications

    APPENDIX 1 - SYSTEM SPECIFICATIONS SPECIFICATIONS: Vessel size 250µmm – 10 mm (rings) or 3mm - 19mm (muscle) Chamber Four individual chambers Chamber material Acid-resistant stainless steel Chamber volume Max. 8ml Chamber suction Manual or automatic, time controlled, user defined Chamber cover Supplied with connections for gassing Chamber gassing...

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