Table of Contents
Advertisement
Quick Links
Advertisement
Table of Contents
Related Manuals for DMT 720MO
Summary of Contents for DMT 720MO
- Page 1 USER GUIDE TISSUE BATH MYOGRAPH SYSTEM - 720MO - USER GUIDE...
- Page 2 TISSUE BATH - 720MO USER GUIDE...
-
Page 3: Table Of Contents
4.4 Maintenance of the linear slides ..............................15 Appendix 1 - Buffer recipes ........................................16 Appendix 2 - Normalization theory ...................................... 18 Appendix 3 - Reading a millimetre micrometer ................................20 Notes ................................................21 TISSUE BATH MYOGRAPH SYSTEM - 720MO - USER GUIDE... -
Page 4: Chapter 1 - Tissue Bath Overview
CHAPTER 1 - TISSUE BATH OVERVIEW Micrometer Transducer house Force transducer pin Pin support connected to micrometer Pin support connected to force transducer Figure 1.1 Tissue Bath with close-up of chamber CHAPTER 1... -
Page 5: Chapter 2 - Setting Up The Tissue Bath
SUPPORTS WILL SAVE TIME WHEN THE MOUNTING SUPPORTS ARE CHANGED AGAIN, MINIMIZING THE AMOUNT OF ADJUSTMENTS NEEDED AFTER EACH CHANGE. Micrometer Figure 2.1 Illustration of the screws for changing supports and adjustment of the pins TISSUE BATH MYOGRAPH SYSTEM - 720MO - USER GUIDE... -
Page 6: Fine Adjusting The Pins For Larger Vessels (Figure 2.2 And 2.3)
2.2 Calibration of the force transducer As a part of the general maintenance of the Tissue Bath, DMT recommends that the Tissue Bath is force calibrated at least once a month. The Tissue Bath should also be force calibrated every time the Multi Interface has been moved. Although lab benches are all supposedly perfectly horizontal, small differences in lab bench pitch can affect the calibration of the system. -
Page 7: Chapter 3 - Experimental Set-Up
3.1 Mounting protocol Below is a short description of how to mount a tissue ring preparation on the mounting pins of the 720MO system. DMT deliver the 720MO system with 200µm pins allowing the user to mount ring preparation with an internal diameter from 450µm and up. -
Page 8: Normalization
3.2 Normalization The importance of normalizing the preparation is three-fold: 1. Experiments with elastic preparations like vessels can only have meaning if they are performed under conditions where the size is clearly defined. 2. Clearly defined conditions are required in pharmacological experiments as the sensitivity of preparations to agonists and antagonists is dependent on the amount of stretch. -
Page 9: Principles Of The Standard Start Procedure
(EDCF). Therefore, it is important to check the existing literature in order to determine the expected response in your particular vessel with the given concentration of agonist. TISSUE BATH MYOGRAPH SYSTEM - 720MO - USER GUIDE... -
Page 10: In Vitro Experiment 1: Noradrenaline Contractile Response
3.5 In vitro experiment 1: Noradrenaline contractile response The purpose of the present protocol is to determine the sensitivity of rat mesenteric small arteries to the vasoconstrictor noradrenaline/norepinephrine with a cumulative concentration-response curve. 3.5.1 Background Noradrenaline (norepinephrine) causes contraction of mesenteric small arteries through activation of α-adrenoceptors whereas noradrenaline activation of β-adrenoceptors causes vasodilatation. -
Page 11: In Vitro Experiment 2: Acetylcholine Relaxation Curve
1.5 μL of 10 1 μL of 10 7.5 μL of 10 1 μL of 10 2.5 μL of 10 *In calculating the [ACh] in the chamber, the applied volume of ACh is ignored. TISSUE BATH MYOGRAPH SYSTEM - 720MO - USER GUIDE... -
Page 12: Chapter 4 - Cleaning And Maintenance
CHAPTER 4 - CLEANING AND MAINTENANCE 4.1 Cleaning the Tissue Bath DMT STRONGLY RECOMMENDS THAT THE TISSUE BATH AND SURROUNDING AREAS ARE CLEANED AFTER EACH EXPERIMENT. At the end of each experiment, use the following procedure to clean the Tissue Bath. -
Page 13: Maintenance Of The Force Transducer
• If the value is 0, a single digit, or a three digit number, the force transducer is broken and needs to be replaced. • If the value is less than 2000 or greater than 4500, the force transducer has been broken and needs to be shipped to DMT for further test and a temperature compensation. -
Page 14: Force Transducer Replacement
4.2.1 Force transducer replacement If the force transducer breaks and needs to be replaced, follow this step-by-step replacement procedure carefully: 1. Remove the pin from the transducer pin coming out of the transducer house. 2. Disconnect the Tissue Bath from the Multi Interface. 3. -
Page 15: Maintenance Of The Linear Slides
Check the linear slides (under the black covers) for grease at least once a week. In case of insufficient lubrication, grease the slides with the “Grease for Linear Slides” included with your system. See figure 4.4. Figure 4.4 The areas where linear slide grease may be applied for smooth micropositioner movement TISSUE BATH MYOGRAPH SYSTEM - 720MO - USER GUIDE... -
Page 16: Appendix 1 - Buffer Recipes
APPENDIX 1 - BUFFER RECIPES Physiological Saline Solution (PSS) 1x PSS: Chemical Mol.Wt g/0.5L g/2L g/4L NaCl (58.45) 3.799 7.598 15.20 30.39 (74.557) 0.175 0.35 0.70 1.40 (136.09) 1.18 0.08 0.16 0.32 0.64 MgSO (246.498) 1.17 0.145 0.29 0.58 1.16 NaHCO (84.01) 14.9... - Page 17 Use the recipe for regular PSS but omit the CaCl and add EGTA (to buffer the residual Ca in solution). The concentration of EGTA in the PSS should be 1-5 mM to ensure sufficient buffering. TISSUE BATH MYOGRAPH SYSTEM - 720MO - USER GUIDE...
-
Page 18: Appendix 2 - Normalization Theory
APPENDIX 2 - NORMALIZATION THEORY The importance of making a normalization before initiating an experiment with any tubular tissue segment is described in chapter 3.2. In this appendix the mathematical rationale and calculations underlying the normalization procedure are described in detail. Mathematical calculations Let (X ) be the pair of values representing the micrometer reading (see appendix 3) and force reading respectively character-... - Page 19 The normalized internal (lumen) diameter is then calculated by: The micrometer reading X at which the internal circumference of the normalized vessel is set to is calculated by: – IC TISSUE BATH MYOGRAPH SYSTEM - 720MO - USER GUIDE...
-
Page 20: Appendix 3 - Reading A Millimetre Micrometer
APPENDIX 3 - READING A MILLIMETRE MICROMETER Sleeve scale Thimble scale Figure A3.1 Overview of the micrometer parts (actual reading 20000 µm = 20 mm) Sleeve scale The micrometer sleeve scale has a total length of 25 mm divided into 50 equal parts. Each part of a division above the horizontal line represents 1 mm, where each 5 line is marked by a longer line and a number designating the length in mm.
Need help?
Do you have a question about the 720MO and is the answer not in the manual?
Questions and answers