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NEW BRUNSWICK SCIENTIFIC AS-10 Manual To Operations

Agar sterilizer
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AS-10
Agar Sterilizer
Models M1189-0010 & M1189-0011
MANUAL NO: M1189-0057
Revision K
May 12, 2003
NEW BRUNSWICK SCIENTIFIC CO., INC.
BOX 4005 • 44 TALMADGE ROAD • EDISON, NJ 08818-4005
Telephone: 1-732-287-1200 • 1-800-631-5417
Fax: 732-287-4222 • Telex: 4753012 NBSCO
Internet: http://www.nbsc.com • E-mail: bioinfo@nbsc.com

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Summary of Contents for NEW BRUNSWICK SCIENTIFIC AS-10

  • Page 1 Models M1189-0010 & M1189-0011 MANUAL NO: M1189-0057 Revision K May 12, 2003 NEW BRUNSWICK SCIENTIFIC CO., INC. BOX 4005 • 44 TALMADGE ROAD • EDISON, NJ 08818-4005 Telephone: 1-732-287-1200 • 1-800-631-5417 Fax: 732-287-4222 • Telex: 4753012 NBSCO Internet: http://www.nbsc.com • E-mail: bioinfo@nbsc.com...
  • Page 2 AS-10 Agar Sterilizer M1189-0057 User’s Guide...
  • Page 3 Fax: 49 (0)7022 32486 E-mail: sales@nbsnv-sa.be E-mail: sales@nbsgmbh.de CHINA THE NETHERLANDS New Brunswick Scientific Co., Inc. New Brunswick Scientific BV Room 1501, Xiangjiang Building, No. 18 Kerkenbos 1101, 6546 BC Nijmegen Lane 1265, Zhongshan Road (W) P.O Box 6826, 6503 GH Nijmegen Shanghai 200051, P.R.
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  • Page 5 Do not use this equipment in a hazardous atmosphere or with hazardous materials for which the equipment was not designed. New Brunswick Scientific Co., Inc. (NBS) is not responsible for any damage to this equipment that may result from the use of an accessory not manufactured by NBS.
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  • Page 7 Reproduction, adaptation, or translation without prior written permission from New Brunswick Scientific is prohibited. Disclaimer Notice New Brunswick Scientific Co., Inc. reserves the right to change information in this document without notice. Updates to information in this document reflect our commitment to continuing product development and improvement.
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  • Page 9 W A R R A N T Y Every Instrument manufactured by the New Brunswick Scientific Co., Inc. is warranted to be free from defects in material and work- manship. This apparatus with the exception of glassware, lamps and electrodes (where supplied), is warranted for 1 year against faulty com-...
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  • Page 11: Table Of Contents

    ....................44 LASS ILTER ONVERSION MAINTENANCE .............................47 ..........................47 OUTINE LEANING .........................47 LEANING XHAUST ..........................48 ERIODIC NSPECTION ..............................48 INGS )..................48 GITATION HAFT EARING ELOW ESSEL ...............................49 ILTER ......................49 ARVEST ALVE SSEMBLY EALS ..........................49 GITATION YSTEM New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 12 Controller Error Messages........................51 ..........................52 ARNING ESSAGES ........................54 ROUBLESHOOTING UIDE REPLACEMENT PARTS & ACCESSORIES .....................57 10.1 .....................57 EPLACEMENT ARTS AND UMBERS 10.2 ........................59 CCESSORY NFORMATION DRAWINGS ..............................61 11.1 ...........................65 IST OF RAWINGS TIMING CHART.............................67 INDEX................................69 AS-10 Agar Sterilizer M1189-0057 User’s Guide...

  • Page 13: Overview

    Unique AS-10 features ensure quality product time after time. An absolute sterile filter is sterilized in place each time the AS-10 is run. Automatic venting eliminates foaming during sterilization and maintains positive pressure during cool-down and dispensing.

  • Page 14: Specifications

    Specifications AS-10 Agar Sterilizer Overall Dimensions Cover Closed Cover Open Height 26 inches 35 inches Depth 20 inches 29 inches Width 24 inches Weight Approx. 160 lbs 12 liters Vessel Total Volume Working Volumes 1 to 10 liters Operating Pressure...

  • Page 15: Vessel Test Certificates

    The inner vessel serial number is stamped on a label located on the outside diameter. The inner vessel must be removed to view the label. New Brunswick Scientific Co., Inc. User’s Guide...
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  • Page 17: Inspection

    Save all packing materials and the User’s Guide. If any part of your order was damaged during shipping, is missing pieces, or fails to operate, please fill out a Customer Satisfaction Form 6300 (copy the form packed with your AS-10) and return it by fax or mail.
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  • Page 19: Introduction

    Physical Location The AS-10 Agar Sterilizer should be placed on a smooth, level and sturdy surface that can support both the sterilizer and its contents when in use. It must also be near suitable electrical, water and drain services. Leave enough clearance to allow easy access to the rear panel, and ensure that the lid and vessel cover are clear from any obstructions that might hinder their proper operation.

  • Page 20: Front Panel Features

    Acquaint yourself with the location (see Figure 1) and features (see Figure 2 on the following page) of the front panel, which is the primary user interface to control your AS- Figure 1: Front Panel Location (top view) FRONT PANEL AS-10 Agar Sterilizer M1189-0057 User’s Guide...

  • Page 21: Process Indication

    Located below the keypad are three green LEDs, to indicate Power, Agitation and Heat. Upon powering-up the unit, the green LED indicating Power should be lit. After starting the sterilization process, the Agitation and Heat LEDs should also be lit. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 22: Keypad/Display Functions

    The setpoint of this function influences the locking function of the protective cover. The protective cover will lock when the temperature rises several degrees above the dispense temperature setting. AS-10 Agar Sterilizer M1189-0057 User’s Guide...
  • Page 23 When the user selects the correct volume, the sterilizer can maintain the correct sterilization temperature within ± 0.5 °C. Failure to input this parameter will result in a possible overshoot or undershoot in temperature of the control process. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 24: Sterilizer Features

    ELECTRICAL SPECIFICATION PLATE ON/OFF POWER SWITCH • ON/OFF POWER SWITCH switch energizes the . The switch is a magnetic ON/OFF POWER AS-10 circuit breaker that will trip if there is an overload or short-circuit. AS-10 Agar Sterilizer M1189-0057 User’s Guide...
  • Page 25 • DRAIN The fitting connects the jacket drain and jacket pressure relief valve to an external drain. • VENT The fitting connects the vessel pressure relief valve to an external drain. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 26: Vessel Cover

    Always respect the tightening sequence in Figure 10. • PRESSURE RELIEF VALVE , set to open at 25 PSIG, prevents excessive PRESSURE RELIEF VALVE pressure build-up in the inner process vessel during sterilization. AS-10 Agar Sterilizer M1189-0057 User’s Guide...
  • Page 27 THERMOWELL TEMPERATURE PROBE the temperature of the sterilization vessel. • TEMPERATURE PROBE ) is provided to sense and to relay the current TEMPERATURE PROBE vessel temperature to the control system and display. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 28: Over-Temperature Safety Thermostat

    Certain agencies require that the vessel be tested to ensure that it is operating within prescribed pressure limits (45 PSIG). The PRESSURE LIMIT TEST PORT used for these tests. It is ¼” NFPT. Figure 6: Top View TEMPERATURE PROBE HOLDER VESSEL COVER PRESSURE LIMIT TEST PORT AS-10 Agar Sterilizer M1189-0057 User’s Guide...

  • Page 29: Making Connections

    3. Attach and clamp the open end of the one-foot hose from the pressure gauge side of the water regulator to the fitting on the rear panel of the AS-10 (see Figure WATER-IN 4, repeated on the following page).
  • Page 30 5. Connect and clamp a 3/8" ID hose, supplied, between the fitting on WATER RETURN the back of the AS-10 and a water recovery system, or run the open end of the hose to an external drain. 6. Connect and clamp a 5/16" ID hose, supplied, to the fitting on the AS-10.

  • Page 31: Electrical Connection

    (located on the rear panel of the unit, see Figure 3). 2. Position the AS-10, and, if you have verified that your electric supply is appropriate, plug it in.
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  • Page 33: Operation

    Before operating the AS-10, all controls in this and the previous chapters should be thoroughly reviewed and understood. Preparation 1. Sterilize the supplied dispense line prior to attaching it to the HARVEST VALVE (see Figure 11). ASSEMBLY SPOOL 2. Using your fingers, loosen the fittings on the...
  • Page 34 9. Fill the vessel with the desired measured amount of medium. The working volume of the vessel is from 1 to 10 liters total volume, including any dry medium. CAUTION! Do NOT overfill, to prevent fluid from being expelled through (and therefore clogging) the sterile filter. AS-10 Agar Sterilizer M1189-0057 User’s Guide...
  • Page 35 . If you are using the TEMPERATURE PROBE THERMOWELL OPTIONAL , ensure that it is correctly connected to your equipment DUAL TEMPERATURE PROBE and install it into the according to the instructions provided with this THERMOWELL option. New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 36 In the up position, a horizontal will SPOOL INDICATOR GROOVE be visible on the of the (see Figure 11 above and SPOOL HARVEST VALVE ASSEMBLY Figure 12 on the following page ). AS-10 Agar Sterilizer M1189-0057 User’s Guide...

  • Page 37: Initial Power-On And Self-Test

    2. Audible alarm is , and all selection LEDs are 3. All selection LEDs are , and all process indication LEDs are 4. All process indication LEDs are 5. Power-on self-test is complete. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 38: Sterilizer Operation

    Sterilizer Operation After the AS-10 is prepared and filled as outlined previously, continue as follows: 1. If the unit is not currently , turn unit using the located ON/OFF POWER SWITCH on the rear of the unit (see Figure 3).

  • Page 39: Removing The Vessel Cover

    This will prevent damage to the bolt threads. 8. After completing step 7, continue loosening all vessel bolts until the VESSEL COVER can be detached from its mating flange. New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 40 VESSEL COVER NOTE: The AS-10 is designed with an outer vessel jacket that is filled with water during operation. After dispensing, when the vessel cover is removed, the inner vessel may pop out of the outer vessel due to the buoyant force on it from the water.

  • Page 41: Communications & Output Ports

    The information provided in this section is for advanced users only. An NBS-certified service technician can help you adjust the settings. In order for the AS-10 Agar Sterilizer to properly communicate to a personal computer that is running BioCommand software (Communications settings: multidropping,...
  • Page 42 Process running mode (see timing chart in the manual for details) "UtiA 4" field In order for the AS-10 Agar Sterilizer to properly communicate to a serial printer, a 3- wire only, no-modem RS-232 cable has to be used. The DIP switches on the main...

  • Page 43: Optional Equipment

    TEMPERATURE PROBE ASSEMBLY The dual probe is available as an ordered option with the AS-10, or as an accessory for those units originally supplied with a single probe. The probe fits in the standard . However, if the probe is ordered as an accessory, it must be installed by THERMOWELL qualified service personnel.

  • Page 44: Glass Wool Filter Conversion Kit

    CENTER SCREEN ASSEMBLY 4. Replace the cap on the filter housing. Figure 16: Glass Wool Conversion Kit Installation CENTER SCREEN ASSEMBLY CENTER HUB FILTER HOUSING ¼” OD TUBE EXHAUST CONNECTOR ASSEMBLY VESSEL COVER AS-10 Agar Sterilizer M1189-0057 User’s Guide...
  • Page 45 5. Remove the existing fitting from the ¼” OD tube of the filter housing (see Figure 17 below) and discard. Figure 17: Fitting Removal ¼” OD TUBING FILTER HOUSING FITTING 6. Install the onto the ¼” OD Tube of the filter EXHAUST CONNECTOR ASSEMBLY housing and hand tighten. New Brunswick Scientific Co., Inc. User’s Guide...
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  • Page 47: Maintenance

    Routine Cleaning The AS-10 should be cleaned immediately after each use, while still warm and before the agar has solidified. To clean the sterilizer: 1.

  • Page 48: Periodic Inspection

    10. Press the button. START/STOP 11. Exhaust line cleaning starts when the AS-10 enters Sterilization mode, and stops when the AS-10 enters Dispense mode. 12. When the vessel cools to dispense temperature, remove the remaining water and turn the power switch off.

  • Page 49: Filter

    1. With the unit empty and the power cord disconnected, tilt the unit on its front side, to protect the unit. 2. Remove the unit’s bottom plate. 3. Inspect the O-ring drive belt on the agitation system for cracks, wear, etc. 4. Replace the O-ring drive belt if necessary. New Brunswick Scientific Co., Inc. User’s Guide...
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  • Page 51: Troubleshooting

    LED or a flashing combination of display parameter LEDs. To determine the code for this type of error, observe which LED parameters are flashing or on the keypad display and compare this to the following table: New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 52: Warning Messages

    128 (see the table on the following pages). In such a case, subtract 128 from the number displayed, then search for the error that corresponds to the remainder. AS-10 Agar Sterilizer M1189-0057 User’s Guide...
  • Page 53 Temperature did not drop from supply is closed. sterilization setpoint to 99º C in • Defective inlet or outlet 15 minutes. solenoid valve on vessel jacket. • Supply water temperature is too hot. …continued… New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 54: Troubleshooting Guide

    Loose heater on jacket. • Defective solenoid valves on vessel jacket. Troubleshooting Guide The following tables list problems that may occur during the use of the AS-10 Agar Sterilizer, and their possible causes: Symptom Possible Cause/Solution • Defective solid state relay.
  • Page 55 Vessel safety relief valve "pops". • Defective temperature sensor. • Defective relief valve. • No response to keypad functions Keypad not connected to control board. Foaming condition. • Leak in vessel seal or exhaust line. New Brunswick Scientific Co., Inc. User’s Guide...
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  • Page 57: Replacement Parts & Accessories

    & & When ordering replacement or accessory parts, or requesting service information, please provide the Model Number, and Serial Number of the AS-10. This information is on the ELECTRICAL located on the rear panel of the unit. SPECIFICATION PLATE 10.1...
  • Page 58 O-ring 2-223 EPRP P0280-6882 O-ring 2-377 EPRP P0280-8242 Bushing, Impeller Guide M1189-9564 O-ring 2-010 VITON P0280-5323 O-ring 2-108 EPRP P0280-5882 O-ring 2-117 EPRP P0280-5972 Filter Absolute Element PTFE P0200-0740 Inoculation Diaphragm MF-468 O-ring 2-113 EPRP P0280-5932 AS-10 Agar Sterilizer M1189-0057 User’s Guide...

  • Page 59: Accessory Information

    Dual Temperature Probe M1189-8009 Glass Wool Filter Conversion Kit M1189-0300 Temperature Probe Validation Kit M1189-0400 QuickConnect Dispense Hose Fitting, ¼ inch M1189-9826 Inner Vessel M1189-6854 Tubing Protector P0740-7500 Duel RTD Temperature Probe M1189-8009 New Brunswick Scientific Co., Inc. User’s Guide...
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  • Page 61: Drawings

    Figure 19a: Control Schematic (see Figures 19b & 19c for details) New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 62 Figure 19b: Control Schematic, Section A AS-10 Agar Sterilizer M1189-0057 User’s Guide...
  • Page 63 Figure 19c: Control Schematic, Section B New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 64 Figure 20: Piping & Instrumentation Diagram AS-10 Agar Sterilizer M1189-0057 User’s Guide...

  • Page 65: List Of Drawings

    Glass Wool Conversion Kit Components Glass Wool Conversion Kit Installation Fitting Removal Spare Parts Kit Spare Parts Kit Control Schematic (overview) Control Schematic, Section A Control Schematic, Section B Piping & Instrumentation Diagram New Brunswick Scientific Co., Inc. User’s Guide...
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  • Page 67: Timing Chart

    New Brunswick Scientific Co., Inc. User’s Guide...
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  • Page 69: Index

    Keypad/Display Functions · 22 Clean Function · 23 Control Schematic · 61 Controller Error Messages · 51 Copyright Notice · 7 Leveling the AS-10 · 31 Curr Temp Function · 22 Location, Preparation · 19 Data Output · 41 Maintenance · 47 DIP Switch Settings ·...
  • Page 70 Steri Time Function · 23 Symbol for · 7 Warning Messages · 52 Warranty · 9 Water In Fitting · 25 Water Return Fitting · 25 Table of Contents · 11 Temperature Probe · 27, 35 AS-10 Agar Sterilizer M1189-0057 User’s Guide...

This manual is also suitable for:

M1189-0010M1189-0011

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