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BioTools BS-2090 Instruction Manual

Inversed biological microscope
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Inversed Biological
Microscope
BS-2090
Instruction Manual
This manual is written for Inversed Biological Microscope BS-2090. For safety and for
exerting the best performance, making you familiar with the instrument entirely, it is
strongly recommended that you read this manual carefully before using the microscope. For
the further reference, please place this manual in a position where nearby the worktable and
fetched easily.
BioTools Inc.

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  • Page 1 BS-2090 Instruction Manual This manual is written for Inversed Biological Microscope BS-2090. For safety and for exerting the best performance, making you familiar with the instrument entirely, it is strongly recommended that you read this manual carefully before using the microscope. For the further reference, please place this manual in a position where nearby the worktable and fetched easily.

  • Page 2: Use Notices

    Bestscope International Limited Use Notices BS-2090 I、 Safety Note 1、 Do not keep the instrument in a direct sunlight, high temperature or humidity, dusty and easy shaking environment. Make sure the stage is plane, horizontal and stable enough. 2、 When moving the microscope, please hold up the instrument with one hand on the lower side of the eyepiece tube ①...
  • Page 3 Bestscope International Limited liquid of the tissue culture entering the inside. 6、 When not using, remember to cover up the microscope with the dust casing. And make sure the lamp cools down enough before you do so. Ⅲ Safety Symbol The surface is very hot, not touch by your hands Before using ,please read the instruction carefully, improper operation will result in bodily injure or instruction malfunction.
  • Page 4 Bestscope International Limited 1. Components BS-2090 Objective Lamp house Phase contrast slider 10X（PH） 20X（PH） Illumination bracket Ocular (eyepiece) Condenser Trinocular viewing tube Stage inserted plate (fixed) Objective turret (Nosepiece) Stage (fixed) (fixed) Microscope base Quintuple Stage lengthen splint Glass stage...

  • Page 5: Installation

    Bestscope International Limited 2．Installation BS-2090 2.1 Installing diagram The following figure shows the installation sequence of the components. The number in the figure shows the installation steps. Before installing, be sure every components is clean, do not score any parts or glass surface.
  • Page 6 Bestscope International Limited BS-2090 2.2 Installing steps 2-2-1 Installing and replacing the lamp （figure3） Please use the specified halogen Lamp 6V30W. Hold to the bulb ①after you wrap it with gauze or other protection materials, then depress the plugs into the jack on the lamp house, ②...
  • Page 7 Bestscope International Limited 2-2-2 Installing the condenser illumination unit（figure 4） Insert the condenser illumination unit into the bracket gently, according the figure showed in ① ② the left. Turn the condenser illumination unit at clockwise about 90°, let the “AS” mark of filter holder③...
  • Page 8 Bestscope International Limited BS-2090 2-2-4 Installing the objective（Fig. 6 and Fig.7） 1. Turning the coarse fusing knob① like the figure shows till the nosepiece get to its lowest position. ensuring safety ★For instruction on transportation, the nosepiece is located in the lowest position and the tension adjustment collar②...
  • Page 9 Bestscope International Limited 2-2-5 Installing the stage lengthen splint and the mechanical ruler（fig. 8） ◎ Stage lengthen splint can be installed in either side of the stage to enlarge the work surface. But you can’t install the mechanical ruler together. ◎...
  • Page 10 Bestscope International Limited BS-2090 2-2-6 Installing the stage inserted plate（fig.9） 1. When using the glass stage ①, there is no special require, you just need to place it in a plane. 2. Install the stage inserted plate on to the stage opening.
  • Page 11 Bestscope International Limited BS-2090 2-2-9 Connecting the power cord（fig.12、13 and 14） Do not bring the power cord to bear a ★ powerful stress. When being bent wrapping, the cable and wires will be broken easily. 1. Turn the main switch① on “O”(off) state before connecting the power cord.
  • Page 12 Bestscope International Limited 3. Adjustment BS-2090 Color filter holder Aperture diaphragm adjust lever Phase center bolt Phase contrast slider Diopter ring Light path selector lever Light switch Main switch Coarse focus knob Fine focus knob Tension adjustment collar Figure 15...

  • Page 13: Operating The Adjustment

    Bestscope International Limited 4. Operating the adjustment BS-2090 4-1 Microscope base 4-1-1 Turning on the lamp （fig.16） Connect the power, turn on the main switch①(shown on the fig.16)which on the bottom side of the base to “－”(on). 4-1-2 Adjusting the brightness （fig.17）...
  • Page 14 Bestscope International Limited BS-2090 4-2 Stage 4-2-1 Setting the specimen （fig.19and fig.20） Set the specimen in the center of the stage, please. ★ to obtain the best observe effect, please select the containers, such as culture dish and culture bottle, with the bottom thickness is 1.2mm, and the same thickness is also required...
  • Page 15 Bestscope International Limited BS-2090 4-3 the viewing tube 4-3-1 Adjusting the diopter（fig.21） Look into the right ocular by your right eye, then revolving the coarse focus knob to focus on the specimen. Then use your left eye to look into the left ocular.
  • Page 16 Bestscope International Limited 4-3-3 Switching the light path（fig.23） ◎ Pulling out the light path selector lever① by your thumb, select the light path you needed. ◎ when in the binocular observation, pushing in the lever until you heard a “clicked” .while in video or photography, pulling out the lever until it reached the “clicked”...

  • Page 17: Illumination Unit

    Bestscope International Limited BS-2090 4-4 Illumination Unit 4-4-1 Using color filters（fig.24） Selecting the appropriate color filters ◎ according your need, it became more effective to observe or photography the specimen. Especially, we suggest using the LBD color filter, which can compensate more neutral colors.
  • Page 18 Best tscope In nternation nal Limit 4-4-2 us sing the ap perture diap phragm（f fig.25） ● When n in the bri ight field obs servation, the e aperture di iaphragm con ntrol the num merical apertu ure of the illum mination syst tem.

  • Page 19: Phase Contrast Viewing

    Bestscope International Limited 5．Phase contrast viewing BS-2090 5-1 The name of the components 5-1-1 Phase contrast objective （fig.26） the optional magnification of the phase ◎ contrast is :10X、20X。 ◎ If you want to know how to mount the phase 2-2-4 contrast objective, please see .
  • Page 20 Bestscope International Limited BS-2090 5-2 The installation and use 5-2-1 Installing the phase contrast slider （ fig.28） 1. Keep the slider① face ( the surface which had character) up towards, then inserted it into the illumination system from the right to the left like the figure showed.
  • Page 21 Bestscope International Limited 4. Using the CT to look the light ring’s image① and the phase contrast ring’s image②, if the light ring’s image is not sharp, please shifting the CT’s ocular until you can see a clear image of the light ring②. 5.
  • Page 22 Bestscope International Limited 6．Microscope photography and video BS-2090 6-1 Microscope video 6-1-1 selecting the light path（fig.31） just used in the trinocular observation ★ Pulling out the light path selector lever, until you heard the “clicked”. ★ In the dark specimen observation, you can make the focus by both eyes at first, then change the light path.
  • Page 23 Bestscope International Limited BS-2090 6-2 Microscope photography 6-2-1 selecting the light path just used in the trinocular observation ★ The operation diagram is shown in 6-1-1， and the details reference is in 4-3-4。 6-2-2 Installing the photography set camera （fig.33）...

  • Page 24: Adjusting The Color Temperature

    Bestscope International Limited 6-2-3 Focus Do the binocular observation at 20% brightness, and focus primary. When in microscope photography, do use the camera viewfinder to focus the specimen. Please refer the user manual of the camera set to obtain the details. 6-2-4 Adjusting the color temperature ◎...

  • Page 25: Technical Specifications

    Bestscope International Limited 7．Technical specifications BS-2090 7-1 Main specifications Optical Infinite Optical System system Compensation Free Trinocular Tube Inclined at 30; Viewing Tube Division ratio: 20% of Binocular Viewing and 80% of Video Viewing & Micrography Eyepiece Wide Field Eyepiece 10X, Linear Visual Field: 22 mm...

  • Page 26: Troubleshooting

    Contrast Objective 8．Trouble Shooting BS-2090 Under certain condition, some no-fault factors will bring a reversible influence to the instrument’s performance. If the problem is happened, please take proper measures according to the follow table. If you can’t solve the trouble by the supplied methods, please contact with the sales department of our company.
  • Page 27 Bestscope International Limited The lens (condenser, objective, not high； The detail is ocular culture dish) l l a become dirty not clear； Don’t obtain phase contrast Use a the culture dish whose phase observation, bottom bottom thickness is less than contrast effect thickness of the culture dish is 1.2mm...
  • Page 28 Bestscope International Limited BS-2090 PROBLEM REASON PAGE SOLUTION II、Mechanical Part: 1.The coarse focus The tension adjustment collar Loose properly knob is hard to run is too tight 2.The image can’t stay on the focal The tension adjustment collar Tighten properly...
  • Page 29 Bestscope International Limited objective 3.The indoor The extra light entered into the Cover up the eyepiece and the window eyepiece and viewfinder is viewfinder of the microscope fluorescence lamp reflected illumination system develop...

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