PSS MCX GPC Quick Start

Quick Start PSS MCX GPC/SEC Columns

1. Introduction

Thank you for choosing a PSS MCX column. It is a high quality, high performance product ready for
immediate use. This Quick Start Guide is designed to get the user up and running quickly with the
common, compatible solvents.
For detailed information on care and use in a wide range of solvents, replacement parts and
accessories, download the Full User's Guide:
www.pss-polymer.com/Resources/manuals/FullUsersGuide_MCX_E.pdf
PSS MCX columns are packed with highly porous particles of a sulfonated styrene-divinylbenzene
copolymer and are supplied as standard in water with 0.75mmol sodium azide. Each column has a
unique serial number and is quality checked for efficiency in the eluent in which the column was
ordered. The test conditions and results are shown on the enclosed certificate of analysis (CoA).
Please keep this in a safe place for future reference.

1.1 Specifications

Column Type
MCX 5µ
MCX 10µ
MCX Prep

1.2 Solvent Compatibility

PSS MCX is compatible with a wide range of aqueous eluents and has optimum performance, when
used with eluents, which have a pH in the range 7.0 to 13.0 for the analysis of sulfonated polyanions.
Transfer to compatible eluents is easy provided the guidelines in this user manual are followed. For
solvents outside this pH range, other PSS column materials may be more appropriate to maintain a
balance between sample ionic character, eluent pH and packing material polarity for a size only based
separation based on the Magic Triangle concept. Please visit the
Parameter
pH Stability
Optimum pH range
Temperature
Organic modifiers
Salts
Buffers

2. Column Installation

When using the column for the first time it is recommended to use the eluent that the column was
originally supplied in and to perform a system plate count noting the backpressure. However, this is
not essential.

2.1 Preparing the GPC/SEC system

Remove any existing columns and store according to the manufacturer's instructions. Connect the
injector directly to the detector with appropriate tubing and connectors. Transfer the GPC/SEC system
from the current eluent to that required, paying attention to the miscibility and compatibility with the
hardware as per the manufacturer's instructions.
PSS Polymer Standards
Service GmbH
In der Dalheimer Wiese 5
55120 Mainz | Germany
Max. Operating Max. Operating
Pressure Pressure for
per column 3 Column Set
bar / psi bar / psi
60 / 870 120 / 1740
40 / 580 80 / 1160
40 / 580 80 / 1160
Range Comment
1-13 Chemical stability
7-13 Due to anionic nature of the surface
<80°C Operate at 10°-35°C for optimum column lifetime
<100% v/v e.g Methanol, Acetonitrile, Tetrahydrofuran
< 0.5M e.g.NaN
Compatible with most commonly used buffers
Phone +49 6131 96239-0
Fax +49 6131 96239-11
E-Mail info@pss-polymer.com
Web www.pss-polymer.com
Max.
Operating
Temperature
80°C
80°C
80°C
PSS website
, NaCl, NaNO
3 3
Polymer Standards
Service-USA, Inc.
160 Old Farm Rd, Suite A
Amherst | MA 01002 | USA
Efficiency
Plates/m
Dextran p1 in
water 0.75mmol
NaN
3
>40.000
>20.000
>15.000
for more information.
Phone +1 413 835-0265
Fax +1 413 835-0354
E-Mail pssusa@pss-polymer.com
Web www.pss-polymer.com