Olink Signature Q100 User Manual
  1. Manuals
  2. Brands
  3. Olink Manuals
  4. Laboratory Equipment
  5. Signature Q100
  6. User manual

Olink Signature Q100 User Manual

Hide thumbs Also See for Signature Q100:
Table of Contents

Advertisement

Quick Links

Download this manual
®
Olink® Signature Q100
User Manual
1172 v1.1, 2022-01-25

Advertisement

Table of Contents
loading

Related Manuals for Olink Signature Q100

Summary of Contents for Olink Signature Q100

  • Page 1 ® Olink® Signature Q100 User Manual 1172 v1.1, 2022-01-25...

  • Page 2: Table Of Contents

    3.3 Best Practices ........................26 4. Olink Signature Q100 Workflows ..................28 4.1 Workflow Overview ......................28 4.2 Olink Target Workflows with the 96.96 and 48.48 chip ........30 4.3 Olink Focus Workflow with the 24.192 chip ............40 5. Troubleshooting .........................48 5.1 Run Instrument Checks ....................50 5.2 Export Instrument Check Logs ...................

  • Page 3: About This User Manual

    1. About This User Manual This user manual describes how to use the Olink® Signature Q100 system, including the instrument and the system software. For complete instrument specifications and unpacking/installation instructions, see Olink Signature Q100 Site Requirements. IMPORTANT: Before using the Olink Signature Q100 system, read and understand the safety guidelines in this document.

  • Page 4: Safety Data Sheets

    Safety Data Sheets Read and understand the SDSs before handling chemicals. To obtain SDSs for chemicals ordered from Olink, either alone or as part of this kit, go to olink.com and search for the SDS using either the product name or the part number.

  • Page 5: System Overview

    The Olink Signature Q100 system provides throughput for proteomics that is higher by orders of magnitude than conventional platforms using Integrated Fluidic Circuits (Chip; from now on referred to as chips)—nanofluidic circuits containing fluidic networks that automatically combine sets of samples with sets of primers.

  • Page 6: Olink Signature Q100 Interface Plates

    Olink Signature Q100 Interface Plates The Olink Signature Q100 interface plates are specific to the type of chip you are using. Store interface plates in the storage container when not in use. The 96.96 Interface Plate comes with the Olink Signature Q100 system.

  • Page 7: Supported Panels

    2.6 Air Options The Olink Signature Q100 system uses compressed air to pressurize fluids and control channels in the chip. The instrument contains an internal compressor that draws in ambient air by default. Optionally connect it to an in-house air source.
  • Page 8 2.7.1 Network Requirements When adding Signature Q100 to network, it is necessary to add it to a secured segmented network that cannot access internet. Olink will not take any responsibilities for the risks associated with networking of the instrument. To connect the Signature Instrument to the network the following requirements must be met: •...

  • Page 9: Regulatory Compliance

    Together with the Firewall, traffic between the networks can be established if the scenario requires it. 2.8 Regulatory Compliance The following directives and harmonized standards were used to evaluate the safety and performance of the Olink Signature Q100 system: 2.8.1 General Regulations and Requirements •...

  • Page 10: Olink Signature Q100 Operation

    Olink Signature Q100 System Software 3.1.1 Unlock the Touchscreen Swipe toward the unlock icon to unlock the touchscreen. 3.1.2 Log In to the Olink Signature Q100 System If required, enter your username and password to log in to the system.
  • Page 11 To access the User Accounts settings, you must have an account that has Manager permissions (see section "3.2 User Accounts"). – Instrument Check: Run instrument checks on the Olink Signature Q100 instrument and export instrument logs. For more information, see section “5.1 Run Instrument Checks”.
  • Page 12 3.1.4 Onscreen Help Olink Signature Q100 includes an on-screen help system. During the process steps of a run, tap to open a new window that contains instructions for how to perform the tasks required for that step of the workflow.
  • Page 13 3.1.5.1 Export Data from a Run 1. Insert a USB drive into the USB port on the front of the instrument. 2. Identify the desired run log in the History screen. 3. Select and export the run files: – Tap in the Export column to select a run, then tap –...
  • Page 14 3.1.5.2 Export Data from Multiple Runs 1. Insert a USB drive into the USB port on the front of the instrument. 2. In the History screen, tap in the Export column to select each run you want to export. 3. Tap (Export).
  • Page 15 2. Tap Yes, delete to delete the selected run folder. If you do not want to delete the run, tap No. A Success message is displayed when the deletion is complete. 3.1.7 Delete Multiple Run Files You can delete the already exported run files. In the History screen, exported runs show a checkmark ( ) in the right column.
  • Page 16 3.1.8 Change System Time Settings 1. Tap Settings > System, then tap (Edit) next to Time Settings. 2. Change the time zone and the date and time, then tap OK.
  • Page 17 3. To view the time in 24-hour format, select the 24 Hour Format checkbox. 4. Tap Save Changes. Tap Yes to update the system settings. If you do not want to keep your changes, tap Cancel.
  • Page 18 3.1.9 Change the Periodic Instrument Check Scheduled Time 1. Tap Settings > System, then tap (Edit) next to Periodic Instrument Check. 2. Change the time of day you want the automated instrument checks to occur, then tap Save Changes. NOTE: The time interval for the automated checks is 30 days and cannot be changed.
  • Page 19 1. Tap Settings > System, then tap (Edit) next to Authentication. 2. Enter a Manager username and password (contact Olink Technical Support if you are the instruments Manager and you don't have access to username and password.). 3. Tap to enable or disable the Require Authentication checkbox, then tap Save Changes.

  • Page 20: User Accounts

    IMPORTANT: To add a new user, enable user authentication, and log in with an account with Manager permissions. 1. Log in to the Olink Signature Q100 system as a Manager. 2. Tap Settings > User Accounts. 3. Tap (New User).
  • Page 21 IMPORTANT: To disable a user account, you must log in with an account that has Manager permissions. 1. Log in to the Olink Signature Q100 system as a Manager. 2. Tap Settings > User Accounts. 3. Tap a user to open the details screen.
  • Page 22 IMPORTANT: To delete a user, you must log in with an account that has Manager permissions. 1. Log in to the Olink Signature Q100 system as a Manager. 2. Tap Settings > User Accounts. 3. Tap a user to open the details screen.

  • Page 23: Best Practices

    Best Practices IMPORTANT: Read and understand the safety information in “10. Appendix: Safety”. For the overall success of the experiment, we recommend the following best practices: 3.3.1 Chip and Control Line Fluid Handling • Use the chip within 24 hr of opening the foil envelope. •...

  • Page 24: Olink Signature Q100 Workflows

    Remove the chip from the instrument and pipet each final sample and primer mix into the chip. Run the chip and collect data on Olink Signature Q100. Transfer the run data using a USB drive or directly import data from the NPX Signature software.

  • Page 25: Olink Target Workflows With The 96.96 And 48.48 Chip

    “3.3.1 Chip and Control Line Fluid Handling”. • Only use Olink® Control Line Fluid for 96.96 chip syringes or Olink Control Line Fluid for 48.48 chip syringes. Control line fluid syringes are prefilled according to a specific chip type. 1. Remove the control line fluid syringes from the packaging and the chip from the box and foil envelope.
  • Page 26 7. Check to ensure that the O-ring returns to its normal position after the syringe is removed. 8. Pull the protective film down and away from the bottom of the chip. Discard the film. 9. Place the appropriate (chip-specific) Olink Signature Q100 interface plate over the chip. Align the barcoded edges. IMPORTANT:...
  • Page 27 4.2.1.2 Prepare the Target 48 or Target 96 chip on Olink Signature Q100 NOTE: If you see the Lock icon, swipe it to unlock it. 1. If required, log into the Olink Signature Q100 instrument. 2. On the Home screen, select the format that matches the chip you are using: –...
  • Page 28 NOTE: If you need to eject the chip, tap (Eject) at the bottom of the screen to return to the Insert a chip with control line fluid into the instrument screen. 6. Tap Start. Preparation begins and displays the time remaining. NOTE: If you need to abort the preparation, tap (Abort) at the bottom of the screen.
  • Page 29 7. When preparation is complete, tap (Eject) in the bottom right corner to open the drawer. 8. Remove the chip and interface plate from the instrument, then tap Close Drawer. IMPORTANT: Do not leave the drawer open while you are pipetting samples and primers into the chip.
  • Page 30 Olink recommends using the reverse pipetting method when pipetting the sample and primer mixes. See the Olink® Target 96 User Manual (Olink 0935) or the Olink® Target 48 User Manual (Olink 1141). 1. Remove the interface plate from the prepared chip.
  • Page 31 Primers Samples Figure 10. Pipetting map for the 48.48 chip 4.2.3 Run the 96.96 or 48.48 chip on Olink Signature Q100 NOTE: If you see the Lock icon, swipe it to unlock the system. 1. If required, login to the Olink Signature Q100 instrument.
  • Page 32 NOTE: Make sure that the protective film has been removed. Barcodes on interface plate and Chip Notched corner in chip and drawer 4. Tap Close Drawer. The system scans the chip and interface plate barcodes, then closes the drawer. 5. On the Add run details screen, confirm the Chip ID, then enter any of the following details for the run: –...
  • Page 33 7. When the run is complete, tap Finish. 8. (Optional) Export your data: a. Insert a USB drive into the USB port on the front of the instrument. b. On the Run Successfully Completed screen, tap (Export Data) to copy the run data to the USB drive. NOTE: You can export the data files later from the History screen.
  • Page 34 9. Tap Open Drawer, then remove the chip and interface plate from the instrument. 10. Tap Close Drawer. 11. Return the interface plate to the storage container. Discard the used chip. 12. Analyze the exported data using the NPX Signature software. NOTE: You can export data onto a USB drive or access it using ethernet (see “2.7 Ethernet...

  • Page 35: Olink Focus Workflow With The 24.192 Chip

    “3.3.1 Chip and Control Line Fluid Handling”. • Only use an Olink Control Line Fluid for a 24.192 chip syringe. Control line fluid syringes are prefilled according to a specific chip type. 1. Remove the control line fluid syringe from the packaging and the chip from the box and foil envelope.
  • Page 36 (Actuation Fluid, Pressure Fluid) into the chip. Note the orientation of the A1 corner. The barcoded edge is on the left side. NOTE: Olink recommends using the reverse pipetting method when pipetting the sample and primer mixes. See the Olink® Target 96 User Manual (Olink 0935) or the Olink® Target 48 User Manual (Olink 1141).
  • Page 37 5. Pipet 20 μL of Pressure Fluid into each of the P4 and P5 inlets ( ) on the chip. 6. Place the Olink Signature Q100 Interface Plate (24.192) over the chip, aligning the barcoded edges. 7. Use clear tape to remove any dust particles or debris from the chip surface, if necessary.
  • Page 38 4.3.3 Run the 24.192 chip on Olink Signature Q100 NOTE: If you see the Lock icon, swipe it to unlock the system. 1. If required, log in to the Olink Signature Q100 instrument. 2. On the Start a New Run screen, tap Focus. NOTE: For more information about the task displayed on the screen, tap 3.
  • Page 39 4. Make sure that the Inteface plate is placed properly on the chip and in level with the drawer. Align the notched corner of the chip to the notch on the drawer and face the barcoded edges of the chip and interface plate forward NOTE: Make sure that the protective film has been removed.
  • Page 40 7. Tap Start Run. The run begins, and the screen displays the time remaining. NOTE: If you need to abort the run, tap (Abort) at the bottom of the screen. See “5.8 Abort a Run in Progress” for more information. 8.
  • Page 41 9. (Optional) Export your data: a. Insert a USB drive into the USB port on the front of the instrument. b. On the Run Successfully Completed screen, tap (Export Data) to copy the run data to the USB drive. NOTE: You can export the data files later in the History screen.
  • Page 42 11. Tap Close Drawer. 12. Return the interface plate to the storage container. Discard the used chip. 13. Analyze the exported data using the NPX Signature software. NOTE: You can export data onto a USB drive or access it using ethernet (see “2.7 Ethernet Option”).

  • Page 43: Troubleshooting

    After cleaning the thermal chuck and chip, retry loading into the system. If the system is still giving an Insufficient vacuum error, contact Olink Technical Support. No or low vacuum. Blocked port(s) of the Clean the Interface plate to remove interface plate.
  • Page 44 (see “5.1 Run Instrument Checks”), then contact Olink® Technical Support. Instrument is in Demo mode. The instrument was left in Contact Olink® Technical Support to Demonstration mode by change the instrument back into Run mistake. mode. Chip scan error. Chip was loaded without its Repeat loading with correct Interface Interface plate on.

  • Page 45: Run Instrument Checks

    Run Instrument Checks You can run two types of instrument checks: • Comprehensive: Checks all components to make sure everything is working correctly (for example, opens and closes valves, raises and lowers the camera, etc.). The comprehensive check takes 8–10 min to complete. •...

  • Page 46: Export Instrument Check Logs

    If the Instrument check fails, contact Olink® Technical Support. Export Instrument Check Logs If Technical Support requests the instrument check logs, export the logs as follows: 1. Insert a USB drive into the USB port on the front of the instrument.

  • Page 47: Shut Down The System

    No fuse replacement is required during installation. If the power fuse is faulty, then replace the fuse: 1. Toggle off the main power switch on the rear of the instrument, then unplug the power cord from the instrument. 2. Remove the fuse holder below the main power switch by pushing and releasing the fuse holder blocks. Main power switch Fuse holder blocks POWERED BY...

  • Page 48: Start Up The System

    The system can remain turned on when not in use. NOTE: If not planned to be used, e.g., for a week, turn off Olink® Signature Q100 to save power. 5.6 Shut Down and Turn Off the System Shut down and turn off the system completely before unplugging it or moving it.
  • Page 49 Power button a. Gently press and hold the power button until the touchscreen screen displays the following message: b. Tap Yes, continue to proceed with system shutdown. NOTE: If a run is in progress, the screen displays a message: NOTE: You can either abort the run and shut down or wait until the run is complete before trying to power down the system again.

  • Page 50: Turn On And Start-Up The System

    The system can remain turned on when not in use. NOTE: If not planned to be used, e.g., for a week, turn off Olink® Signature Q100 to save power. 5.8 Abort a Run in Progress IMPORTANT: Aborted runs cannot be restarted.

  • Page 51: Idle Mode

    2. Tap Open Drawer, then remove the chip and interface plate. 3. Tap Close Drawer to return to the Home screen. Idle mode The instrument goes into idle mode automatically when there is no user interaction for a long while. Touch the screen to take the instrument out of its idle mode.

  • Page 52: Preventive Maintenance, Decontamination, And Disposal

    For optimal performance of your Olink Signature Q100 system, we recommend: • Performing annual maintenance by a certified Olink service engineer. • Ordering a replacement interface plate from Olink when the plate approaches its life expectancy of 2,000 runs. • Only using replacement parts supplied by Olink.
  • Page 53 • Do not use bleach to clean the instrument as it is corrosive to metal. • Before using a cleaning or decontamination method other than those recommended by Olink®, verify with Olink Technical Support that the proposed method will not damage the equipment.
  • Page 54 BMBL guidelines online at cdc.gov/biosafety/publications/index.htm. Ensure that the Olink Signature Q100 system is cleaned or decontaminated before servicing the equipment, removing it from use, or transporting it for disposal. Refer to the instructions contained in this document and use only those materials specified.
  • Page 55 Do not dispose of this product in unsorted municipal waste. This equipment may contain hazardous substances that could affect health and the environment. Use appropriate take-back systems when disposing of materials and equipment. Learn more at olink.com.

  • Page 56: Technical Support

    7. Technical support For technical support contact support@olink.com.

  • Page 57: Revision History

    8. Revision history Version Date Description 2022-01-25 Added text to section Editorial changes 2021-11-11...

  • Page 58: Appendix: Related Documents

    Olink® Target 96 User Manual 0935 Olink® Target 48 User Manual 1141 Olink® Signature Q100 Site Requirements Guide 1170 Olink® NPX Signature User Manual 1173 Olink® Signature Q100 Installation Guide 1171 Olink® Signature Q100 - A benchtop system for protein biomarker analysis 1164...

  • Page 59: Appendix: Safety

    10. Appendix: Safety 10.1 General Safety In addition to your site-specific safety requirements, Olink recommends the following general safety guidelines in all laboratory and manufacturing areas: • Use the appropriate personal protective equipment (PPE): safety glasses, fully enclosed shoes, lab coats, and gloves, according to your laboratory safety practices.
  • Page 60 Follow local municipal waste ordinances for proper disposal provision. Contact customer service for information about responsible disposal options. POWERED BY FLUIDIGM COMPRESSION AIR INLET (OPTIONAL) 70–90 PSI ONLY AC 100–240V 50–60Hz, 8A Rear of instrument Figure 16. Safety symbols on the Signature Q100 instrument 10.3 Electrical Safety...
  • Page 61 NOTE: The main power disconnect is on the rear panel of the instrument. ARNING: ELECTRICAL HAZARD. DO NOT REMOVE THE COVERS. Electrical shock can result if the system is operated without its protective covers. No internal components under the covers are serviceable by the user. ARNING: ELECTRICAL HAZARD.
  • Page 62 For Research Use Only. Not for use in diagnostic procedures. All information in this publication is subject to change without notice. Trademarks: Olink and the Olink logo are trademarks or registered trademarks of Olink Proteomics AB in the United States and/or other countries. All other trademarks are the sole property of their respective owners.

Table of Contents