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Summary of Contents for Consort E3100
- Page 1 Consort MANUAL Distributor: Topac Inc 231 CJC Highway Cohasset MA 02025 E3100 E3200 Tel 781 740 8778 E3300 Fax 781 740 8779 E3400 www.topac.com E3500 E3600 email sales@topac.com July 2009...
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Page 2: Table Of Contents
Fitting electrode cables ..........4 Fitting loading guides ..........4 Gel preparation ............5 Gel pouring with casting dams ........6 Gel pouring with model E3100........6 Gel pouring using tape method........7 Gel pouring using the flexicaster........7 Running the gel ............8 Gel staining and viewing .......... -
Page 3: Warranty
No liability is accepted for loss or damage arising from the incor- rect use of this unit. CONSORT’s liability is limited to the repair or replacement of the unit or refund of the purchase price, at CONSORT’s option. -
Page 4: Safety Precautions
Safety precautions Safety precautions • When used correctly, these units pose no health risk. • However, these units can deliver dangerous levels of electric- ity and are to be operated only by qualifi ed personnel follow- ing the guidelines laid out in this manual. Anyone intending to use this equipment should read the com- •... -
Page 5: General Care And Maintenance
General care and maintenance General care and maintenance • Units are best cleaned using warm water and a mild deter- gent. Water at temperatures above 60°C can cause damage to the unit and components. The tank should be thoroughly rinsed with warm water or distilled water to prevent build up of salts but care should be taken not to damage the enclosed electrode and vigorous cleaning is not necessary or advised. -
Page 6: Fitting Electrode Cables
Fitting electrode cables Fitting electrode cables 1. Note the position of the lid on the unit. This shows the cor- rect polarity and the correct orientation of the cables, black is negative and red positive. 2. Remove the lid from the unit. Note if the lid is not removed, fitting the cables may result in un-tightening of the gold plug and damage to the electrode. -
Page 7: Gel Preparation
For a standard 0.7 % agarose gel, add 0.7 g of agarose to 100 ml of 1x TAE or TBE solution. The same 1x solution should be used in the tank buffer solution. Model Gel size (cm) Volume (ml) E3100 10x8 E3200 7x10 E3300 10x7... -
Page 8: Gel Pouring With Casting Dams
5. Carefully remove the gel casting gates and comb and transfer the gel including tray to the main tank. the gel including tray to the main tank. Gel pouring Gel pouring with model E3100 Gel pouring with model E3100 Gel pouring Gel pouring with model E3100... -
Page 9: Gel Pouring Using Tape Method
Gel pouring Gel pouring using tape method using tape method 1. Autoclave or plastic backed general tape should be used. A length 5 cm longer than the width of each end of the tray should be cut. One length should be placed over one end of the tray folded and the edges sealed securely. -
Page 10: Running The Gel
Gel staining and viewing Gel staining and viewing All trays and the E3100 unit allow staining to be performed with- out removing the gel from the tray if this is preferred. 1. Transfer the gel to a vessel containing the appropriate vol- ume of 0.5 µg/ml ethidium bromide stain for 15...30 minutes,... -
Page 11: Solutions
Solutions Solutions 1x TAE 40 mM tris (pH 7.6), 20 mM acetic acid, 1 mM EDTA • 50 x (1l) dissolve in 750 ml distilled water: • 242 g tris base (FW = 121) • 57.1 ml glacial acetic acid •... -
Page 12: Troubleshooting
Troubleshooting Troubleshooting Equipment problem • Bubbles do not appear on the electrodes: Ensure that the power supply and the whole electric assembly is operating properly. • Melted agarose leaks when casting: Ensure the agarose is not too hot when poured. Ensure that the sealing surfaces of the running tray and the gel casting gates are clean. - Page 13 • Bromophenol blue dye turns yellow: Check pH of buffer during electrophoresis. (pH change). Ensure Tris base and not Tris-HCl was used. Mix the buffer periodically during electrophoresis. Connect a pump to circulate the buffer. • Double-banded pattern Ensure the comb is vertical during casting so that the well shape is not distorted.
- Page 14 E3xxx E3xxx...
- Page 15 DECLARATION OF CONFORMITY We declare under our sole responsibility that the product Horizontal Apparatus content of the type numbers E3100, E3200, E3300, E3400, E3500, E3600 to which this declaration relates is in conformity with the following standards EN61010 LOW VOLTAGE DIRECTIVE 73/23/EEG...
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