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Summary of Contents for Consort EVS1000 Series
- Page 1 EVS1000 BLOT series MANUAL Rev. 44/2019...
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Page 3: Table Of Contents
Contents Contents ............................................. b Safety Information ........................................ 1 Precaution ........................................... 1 General care and maintenance ..................................1 Environmental conditions ....................................2 Fitting electrode cables ..................................... 2 Electroblotting......................................... 3 Setting up the blot sandwich ..................................3 Blot run conditions ......................................3 Buffer solutions for blotting .................................. -
Page 4: Safety Information
EVS1000 Blot units Safety Information Precaution • When used correctly, these units pose no health risk. • However, these units can deliver dangerous levels of electricity and are to be operated only by qualified personnel following the guidelines laid out in this instruction manual. •... -
Page 5: Environmental Conditions
EVS1000 Blot units Environmental conditions • This apparatus is intended for indoor use only. • This apparatus can be operated safely at an altitude up to 2000 m. • The normal operating temperature range is between 4°C and 65°C. • Maximum relative humidity 80 % for temperatures up to 31°C decreasing linearly to 50 % relative humidity at 40°C. -
Page 6: Electroblotting
EVS1000 Blot units Electroblotting Setting up the blot sandwich Each blot sandwich should be set up as follows: • Cassette clamp [-ve] (black) side placed in a tray or other suitable surface. • Pre-soaked fibre pad (note two can be used with thin gels). •... -
Page 7: Buffer Solutions For Blotting
EVS1000 Blot units buffer and thickness and percentage of gel. This will also affect quality of transfer so time course of transfer should be performed for your particular samples and conditions. When the blot time is completed, turn the power supply off. Remove the cassettes from the main tank. -
Page 8: Protein (Western) Blotting Buffers
EVS1000 Blot units Protein (Western) blotting buffers Transfer buffers must be made accurately using high grade reagents. Do not adjust the pH with acid or base as this will affect the properties of the buffer. pH will vary according to the purity of the reagents used. -
Page 9: Certificate
EVS1000 Blot units Certificate... -
Page 10: Warranty
If any defects occur during this warranty period, Consort will repair or replace the defective parts free of charge. This warranty does not cover defects occurring by accident or misuse or defects caused by improper operation. - Page 11 EVS1000 Blot units...
- Page 12 EVS1000 Blot units Consort bvba Hertenstraat 56 unit 9 2300 Turnhout Belgium : (+32) (0)14 41 12 79 : (+32) (0)14 42 91 79 Sales : sales@consort.be Support : support@consort.be Information : info@consort.be...
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