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BFP SYSTEM : Quick Starting Guide
BUNDLE IMAGING
FIBER PHOTOMETRY
'BFP' SYSTEM
QUICK STARTING GUIDE
© DORIC LENSES INC
357 rue Franquet - Québec, (Québec)
G1P 4N7, CANADA
Tel. : (418) 877-5600 - Fax. : (418) 877-1008
www.doriclenses.com
Date: 2020-02-28
Rev: 00
Copyright © 2020 Doric Lenses Inc. All Rights Reserved.
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Summary of Contents for DORIC BFP

  • Page 1 QUICK STARTING GUIDE © DORIC LENSES INC 357 rue Franquet - Québec, (Québec) G1P 4N7, CANADA Tel. : (418) 877-5600 - Fax. : (418) 877-1008 www.doriclenses.com Date: 2020-02-28 Rev: 00 Copyright © 2020 Doric Lenses Inc. All Rights Reserved. 1 / 15...
  • Page 2 There is also 3 Digital Input or Output to synchronize the BFP system with other equipment. Since each camera, the BFP driver and the LED driver are connected to a computer via USB cables, a 4 ports USB 3.0 hub is included to connect only 1 USB cable to the computer..
  • Page 3: Software Installation

    2.1. BEFORE connecting anything to the computer, you need to install the camera driver that is on the USB key supplied with the system. Launch Cam33U_setup_4.7.0.1557.exe. 2.2. Install Doric Neuroscience Studio, using the version on the USB key supplied with the system; Launch setup_DoricStudio5.4.0.15.exe Or looks for the most recent release in our website : http://doriclenses.com/life-sciences/software/955-doric-neuroscience-studio.html...
  • Page 4 3.2. Bundle imaging Fluorescence Mini Cube 'BFMC' This is the central part of the BFP system. It contain set of dicrhroic mirrors and bandpass spectral filters with optics to have uniform illumination on optical fibre port that is re-imaged to an image snesor.
  • Page 5 BFP SYSTEM : Quick Starting Guide 3.3. BFP Driver 'BFPD' The BFP Driver 'BFPD' synchronize the LED driver with camera acquisition in an interleave mode. There is also 3 Digital Input or Output to synchronize the BFP system with other equipment.
  • Page 6 4.1. Load configurations files If a configuration already exist, you may load the configuration by clicking Load Configuration buttons in the LED driver and the BFP driver tabs (2 different files). 4.2. Set LED driver Turn ON the LED Driver, and set each channel to TTL by pushing the knob 2 times, then adjust the current to the desired value by rotating the knob.
  • Page 7 LED will turn OFF completely. 4.3. BFP Set New Configuration (Configuration Tab) In the BFP Driver tab, go to Configuration tab, and click + NEW/EDIT button. Copyright © 2020 Doric Lenses Inc. All Rights Reserved. 7 / 15...
  • Page 8 LIVE or RECORD in Acquisition tab. If you want to have the recording start when triggered by an external signal via a Digital Input, as trigger source, there is 2 possibilities; Copyright © 2020 Doric Lenses Inc. All Rights Reserved. 8 / 15...
  • Page 9 DIO is 'TTL-High', then data stream will interrupt when the DIO is 'TTL-low', but the time will continue, and when the DIO is back to 'TTL-High', the recording will continue until you manually click STOP in the software interface. Copyright © 2020 Doric Lenses Inc. All Rights Reserved. 9 / 15...
  • Page 10 - When ROI are defined, we suggest to save them using the 'Save' button in the ROI tab for faster setup next day, and lock the editing to avoid moving them by mistake. Copyright © 2020 Doric Lenses Inc. All Rights Reserved. 10 / 15...
  • Page 11 Since you can define independant set of ROI for each camera, it is not required to center both camera field of view, but it improve visualisation and could facilitate post-processing. Copyright © 2020 Doric Lenses Inc. All Rights Reserved. 11 / 15...
  • Page 12 * Please note that if no ROI have been set, nothing will be recorded. Copyright © 2020 Doric Lenses Inc. All Rights Reserved. 12 / 15...
  • Page 13 12 hours, as per example above. This will create a single file with all the datas, NOT a file for each serie. Copyright © 2020 Doric Lenses Inc. All Rights Reserved. 13 / 15...
  • Page 14 4.8. Additional DIO (3) To be documented. More details on how to use the softare to come... Copyright © 2020 Doric Lenses Inc. All Rights Reserved. 14 / 15...
  • Page 15: Focus Adjustment

    * Be careful, you may need to rotate several turns to adjust focus. To avoid twisting the cable, you may need to disconnect and re-connect optical fiber cable during this alignment process. Bundle patchcord image in focus (negative) Copyright © 2020 Doric Lenses Inc. All Rights Reserved. 15 / 15...