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TAKARA PCR TP350 User Manual

Thermal cycler dice touch
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TaKaRa PCR
TM
Thermal Cycler Dice
Touch
Code TP350
User's Manual
for Software version 2.0.1
TAKARA BIO INC.
・Users are recommended to carefully read the contents of this manual before operating
Attention
TM.
the TaKaRa PCR Thermal Cycler Dice
Touch
・Carefully observe all special Warnings and Cautions outlined in this manual.
・This manual should be stored for reference in a secure and accessible location.

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Summary of Contents for TAKARA PCR TP350

  • Page 1 TAKARA BIO INC. ・Users are recommended to carefully read the contents of this manual before operating Attention the TaKaRa PCR Thermal Cycler Dice Touch ・Carefully observe all special Warnings and Cautions outlined in this manual. ・This manual should be stored for reference in a secure and accessible location.
  • Page 2 This product is covered by the claims of U.S. Patent 5,552,580, and their corresponding foreign counterpart patent claims. ・Thermal Cycler Dice is a trademark of TAKARA BIO INC. ・All marks are the property of their respective owners. Certain trademarks may not be registered all jurisdictions.

  • Page 3: Table Of Contents

    TP350 User’s Manual TABLE OF CONTENTS page Precautions for Using TaKaRa Scientific Instruments --- 2 Chapter 1: Overview ------------------------------------ 7 Chapter 2: Installation Requirements ----------------------- 9 1. Installation Conditions 2. Installation 3. Moving the System Chapter 3: Names of Parts and Their Functions...

  • Page 4: Precautions For Using Takara Scientific Instruments

    TP350 User’s Manual Precautions for Using TaKaRa Scientific Instruments ● Users are recommended to carefully read the content of this manual before operating the TaKaRa PCR Thermal Cycler Dice Touch. ● After familiarizing themselves with the information in this manual, users are recommended to store it in...
  • Page 5 TP350 User’s Manual （２）General Precautions Regarding TaKaRa Scientific Instruments It is important to observe the various precautions described below to ensure optimum system performance and prevent accidents before they happen. Ensure safe and correct system operation by carefully reading the precautions.
  • Page 6 TP350 User’s Manual ● Be sure to compare system power supply requirements with the rated ！ WARNING power capacity of the power source to ensure system current requirements do not exceed power socket capacity. Confirm that the total power requirements of all connected equipment do not exceed the rated power supply capacity of the common power source.
  • Page 7 TP350 User’s Manual （３）Warning Safety Labels Applied to System Case Users are advised to avoid accidents by carefully reading the warnings and cautions contained on warning stickers at key locations on the interior and exterior of the system. Warning Type Possible Location of Danger Warning Label...
  • Page 8 Precautions against Transferring or Copying Content of This Document The content of this document cannot be transferred or copied without the express permission of the copyright holder. Copyright holder: TAKARA BIO INC. Printed in Japan Contact TAKARA BIO INC. TAKARA BIO INC.

  • Page 9: Chapter 1: Overview

    Thank you for purchasing TaKaRa PCR Thermal Cycler Dice Touch. TaKaRa PCR Thermal Cycler Dice Touch is more small PCR device and easy to use the big touch panel , that reconfigure TaKaRa PCR Thermal Cycler Dice series as usual. This...
  • Page 10 Save and transfer PCR run files and logs between the default or individual user directories and a USB memory drive.  A facility has provided to update the software which resides on the TaKaRa PCR Thermal Cycler Dice Touch with USB memory stick.

  • Page 11: Chapter 2: Installation Requirements

    TP350 User’s Manual ●Chapter 2: Installation Requirements 1. Installation Conditions To ensure optimum performance and long and stable product life, observe the following precautions when selecting an installation location. Note that installing the system in a location that does not meet the requirements listed below can result in malfunctions and/or system breakdown.

  • Page 12: Installation

    TP350 User’s Manual 2. Installation Use the following procedures when installing system. 1. Confirm that the system power switch is set to off. 2. Connect the system side of the power cable to the power connector on the rear of the system. 3.

  • Page 13: Chapter 3: Names Of Parts And Their Functions

    TP350 User’s Manual ● Chapter 3: Names of Parts and Their Functions Front View ①Lid Lock ②Lid Handle ③Lid ④Touch Panel ⑥Air vents （exhaust; Left and Right sides ） ⑤USB Port ⑦Stylus（Touch Pen） General Description ―――――――――――――――――――――――――――――― ①Lid Lock The Lid Lock is slided behind the unit, so that the Lid picks a Lock.
  • Page 14 TP350 User’s Manual Lid Block View ⑧Top Heater ⑨Sample Block ⑬Backside USB Port Rear View ⑩Power Switch ⑪Power Cable Connector General Description ⑫Air vents (intake) ―――――――――――――――――――――――――――――― ⑧Top Heater The Top Heater protects against evaporation resulting from heating of the reaction tube and reaction plate. 【Caution】...

  • Page 15: Chapter 4: System Operation

    Electrical AC100V～240V, 50/60Hz Consuming electric power 500VA （5A @100V） 1-1. Power Switch On Power up the TaKaRa PCR Thermal Cycler Dice Touch will display the logo and take a few moments to initiate the software. 【Caution】 Please do not touch the Touch panel and shut down the system until the main menu appear on the panel screen.
  • Page 16 TP350 User’s Manual 1-3. Home After power up the unit defaults to the home screen from where all features may be accessed. The 4 screen icons provide access the following features: ① PCR Program Editor- is used to create a new PCR thermal profile and initiate its execution.

  • Page 17: Pcr Profile Creation

    TP350 User’s Manual 2．PCR Profile Creation From the ‘home’ screen the ‘PCR Program Editor’ icon will bring you to appear an initial display of PCR program. Create a new thermal profile using either the initial display or the Wizard mode to click witch hat icon (worn Wizard) under the screen. An Active step become blue in a color on the program.
  • Page 18 TP350 User’s Manual ２－１． Configure and Edit a new thermal profile Appear an initial profile that is a default profile or a profile for previous measure- ment in PCR Program Editor. If you want to display a default profile but not a profile for previous measurement, you would click hat icon (worn Wizard) under the screen and open the default profile.
  • Page 19 TP350 User’s Manual ２－２． Configure a new profile using Wizard Mode The TaKaRa PCR Thermal Cycler Dice Touch software includes a Wizard utility which enables the user to configure easy to moderate complexity profiles in just moments. To activate select ‘Wizard’ from the PCR or One Step RT-PCR start-up screen.
  • Page 20 TP350 User’s Manual ２－３． Explanation of functional icons for editing the profile ＜Creating a new Event using Add Step＞ To create a new event select an ‘Add Step’ icon in the ‘PCR Program Editor’. The first screen of the Add Event Wizard which will be displayed lets the user select the type of event.
  • Page 21 TP350 User’s Manual ＜Explanation of specific function (Advanced Edit)＞ Within the cycling configuration screens there are numerous options. There are also options for Gradient , Touchdown and Long range features to use the Advanced Edit. Gradient : allows both the left and right temperatures (range of temperature values: 4～99℃) that is programmable 0～25℃...
  • Page 22 TP350 User’s Manual ２－４．Confirm Run When the user is happy with the profile parameters just click on the tick. A run review screen will now be shown with a text description of the profile steps. Click on OK to commence run execution. Once the profile is confirmed, if the ‘Use Top Heater’...
  • Page 23 TP350 User’s Manual When the run commences the software will automatically proceed to the ‘Run’ screen. You are not able to modify the profile once a run has commenced. You are able to skip a step , pause and stop using right icons on the Run Screen. On run completion the post run report screen will show and if enabled an audible alarm will sound.

  • Page 24: Pcr File Manager

    USB memory drive. Plug your USB drive into the connector on the front of the unit and you may browse the directories on the Takara Thermal cycler Dice Touch and copy/paste files to or from.
  • Page 25 TP350 User’s Manual ＜PostRunReport＞ The post run report can be saved as a PostRunReport text file on the PCR File Management (maximum recent 10 files). Specification is described content of a PostRunReport file. v.2.01...

  • Page 26: Manual Incubation

    TP350 User’s Manual 4. Manual Incubation The manual incubation mode screen allows immediate manual setting of the block temperature. To activate this mode simply press the blue ‘start’ button. Terminating the mode is achieved by pressing the red ‘stop’ button. To program the set temperature press the ‘Temp’...

  • Page 27: Configuration Tools

    ⑤User Accounts： User account control. ⑥Software Update： Allows upgrading of the Takara Thermal cycler Dice Touch operating Software. ⑦Touch panel calibration： You can also perform a calibration of the touch-panel interface using the calibration wizard.
  • Page 28 TP350 User’s Manual ５－１．System Information Confirm a general information screen which includes such information as serial number, software （ N-xxxx ） and hardware version numbers and Controller Software Version. ５－２．User Accounts In the users area you are able to select an existing user or create or delete a user profile.
  • Page 29 Use predicted liquid temperature for hold times： The Takara Thermal cycler Dice Touch software implements a thermal model which predicts the actual liquid temperature inside the reaction vessels (Sample volume is 10～50 μl).
  • Page 30 【Note】 The Top Heater is only activated when a run is started or the operator enables it in manual mode. ５－４．Software Update A facility has been provided to update the software which resides on the Takara Thermal cycler Dice Touch. The upgrade file you receive from our support team should be placed on the main root directory of your USB memory stick.

  • Page 31: Chaptur 5: Diagnosis Functions

    TP350 User’s Manual ●Chapter 5: Diagnosis Functions The following are provided in three test files to program of ‘Dice’ account folder.  Cycle Test： Test cycling time and stability of control operations  Up Down Test： Measures time required from heat up to 98℃ to cool down to 20℃...
  • Page 32 TP350 User’s Manual ④ Activating ’Run File’ button under the icons will show a profile review page which allows the operator to check over the profile (displayed in text format) before confirming commencement and then click ‘Start Run’. ⑤ The Program is started. ⑥...

  • Page 33: Up Down Test

    TP350 User’s Manual 2. Up Down Test ① From the ‘home’ screen the ‘PCR File Manager’ icon will bring to the experiment page. You can load an existing experiment from the internal drive. ② Click the ‘Dice’ icon from some accounts ③...
  • Page 34 TP350 User’s Manual ⑥ The Program is started. ⑦ The below report is described to the end of Up Down Test program. Run Started, Run completed and Run duration is appeared. The report is saved a PostRunReport file ⑧ You checked that the run durations less than the Standard Value. The Reference Value：less than 5 minutes 30 second (Cycle time) v2.01...

  • Page 35: Ramp Test

    TP350 User’s Manual 3. Ramp Rate Test ① From the ‘home’ screen the ‘PCR File Manager’ icon will bring to the experiment page. You can load an existing experiment from the internal drive. ② Click the ‘Dice’ icon from some accounts ③...
  • Page 36 TP350 User’s Manual ⑥ The Program is started . ⑦ The below report is described to the end of Ramp Rate Test program. ⑧ Click the lower right check then the ‘Configuration’ is selected from the ‘home’ screen. v2.01...
  • Page 37 TP350 User’s Manual ⑨ Select the ‘Thermal Calibration’ from the ‘Configuration’ menu. ⑩ Touch the ‘Ramp Rate’ button on the Calibration Wizard screen. ⑪ The Max Cool rate and Max Heat rate is displayed on the System Massage Reference Value: Max Cool is more than 2.5℃/sec Max Heat is more than 3.0℃/sec ⑫...

  • Page 38: Chapter 6: Troubleshooting And Maintenance

    TP350 User’s Manual ●Chapter 6: Troubleshooting and Maintenance If a problem with Thermal Cycler Dice Touch arises the table below outlines the troubleshooting steps that should be followed and the possible solutions to the problem. Problem Possible Cause Solution Unit does not power up. The power cable is unplugged.
  • Page 39 TP350 User’s Manual Problem Possible Cause Solution Warning message “Block Sensor The Main block is malfunctioning. Need to replace the Main Block. Failure” is displayed on screen indicating a block sensor failure. Warning message is displayed on The Main block may be If problem occurs repeatedly screen indicating “system has malfunctioning.
  • Page 40 TP350 User’s Manual Power failure Power failure occurred on the program running and then power was recovered. The machine was resumed and the following message ‘Care should appeared on the screen. When touch the OK button , start to run the program from the stopped state. When a heated top heater becomes by power failure below in a predetermined temperature, resume operation after warming a heated top heater to an...

  • Page 41: Chapter 7: Specifications

    TP350 User’s Manual ● Chapter 7： Specification (＊)Performance specification apply when system is operated in the 20 to 23 degree C temperature range. Product name TaKaRa PCR Thermal Cycler Dice Touch Model No. TP350 Ambient temperature 15℃～30℃ Operating environment Humidity 20% ～ 80% RH...

  • Page 42: Appendix 1: Accessories List And Packing

    1. Power cable 2. User’s Manual 3. Stylus (Touch pen) 4. Warranty sheet 5. TaKaRa Supplied PCR Tubes trial set < Single Tube > 0.2 ml Hi-Tube Dome Cap Single Tube (Cat.# NJ200) < 8 Tube & Cap > 0.2 ml Hi-8-Tube 8 Tube (Cat.# NJ300 )

  • Page 43: Appendix 2: Consumable List

    TP350 User’s Manual ● Appendix 2: Consumable list List of Supplies（Tubes and Plates） Cat. # Product Name Quantity 0.2 ml Single Tube NJ200 0.2 ml Hi-Tube Dome Cap 1,000 NJ201 0.2 ml Hi-Tube Dome Cap Recovery※ 1,000 NJ202 0.2 ml Hi-Tube Flat Cap 1,000 NJ203 0.2 ml Hi-Tube Flat Cap Recovery※...

  • Page 44: Appendix 3: Servicing Request And Safety Check

    TP350 User’s Manual Servicing Request and Safety Check Attention: Distributor of Takara Bio Inc. Product Name： TaKaRa PCR Thermal Cycler Dice Touch Model : TP350 Serial No Date of request： Requested by Name Contacts： （Telephone、Email） Company/Institute Name Address Details of request：...
  • Page 45 TP350 User’s Manual MEMO ____________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________ ____________________________________________________________________________________________________________________________________ NAME____________________________________________________________________ ｖ2.01...
  • Page 46 SETA3-4-1, OTSU, SHIGA, 520-2193 URL: http://www.takara-bio.com 2015.03.09...

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