Related Manuals for VWR avantor VisiScope 400 Series
Summary of Contents for VWR avantor VisiScope 400 Series
- Page 1 Instruction manual Microscope VisiScope Series 400 ® EU cat. no 630-3254 630-3256 630-3255 VERSION: 02 ISSUED: 03/2021...
- Page 2 LEGAL ADDRESS OF MANUFACTURER Europe VWR International bv Researchpark Haasrode 2020 Geldenaaksebaan 464 B-3001 Leuven + 32 16 385011 be.vwr.com UK Importer VWR International Ltd Hunter Boulevard, Magna Park Lutterworth, Leicestershire, LE17 4XN uk.vwr.com Country of Origin Italy vwr.com | Instruction manual...
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Page 3: Table Of Contents
10.8 Stage and stage inserts 10.8.1 Installing stage inserts 10.9 Aperture diaphragm 10.10 Using color filters Use of the microscope in Phase Contrast (transmitted light) 11.1 Installing the phase contrast slider 11.2 Phase contrast slider 11.3 Centering the phase ring Instruction manual | vwr.com... -
Page 4: Warning
Users should observe all safety regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit. vwr.com | Instruction manual... -
Page 5: Package Contents
Stage inserts LED illuminator Centering telescope Anti-glow cap Green filter IF550 Mechanical stage Lens cleaning tissue UV shield Phase contrast slider Glass insert for stage Dust cover Metal insert for stage Power supply + power cord Objectives Instruction manual | vwr.com... -
Page 6: It406 Fl4
3.2 IT406 FL4 Microscope body Metal insert for stage Condenser Mechanical stage LED illuminator UV shield Objectives Stage inserts Eyepieces Lens cleaning tissue Dust cover Anti-glow cap Glass insert for stage Power supply + power cord vwr.com | Instruction manual... -
Page 7: It406 Fl4D
“C” mount LED illuminator Mouse Anti-glow cap Keyboard Objectives Monitor Eyepieces UV shield Glass insert for stage Power supply + power cord Metal insert for stage Micrometric slide Mechanical stage Lens cleaning tissue Dust cover Stage inserts Instruction manual | vwr.com... -
Page 8: Unpacking
The following chart is an illustrated glossary of the symbols that are used in this manual. CAUTION This symbol indicates a potential risk and alerts you to proceed with caution ELECTRICAL SHOCK This symbol indicates a risk of electrical shock. vwr.com | Instruction manual... -
Page 9: Instrument Description
CONDENSER DIOPTER ADJUSTMENT RING FILTER HOLDER PHASE CONTRAST SLIDER STAGE PLATE STAGE LIGHT PATH SELECTOR LEVER OBJECTIVE FLUORESCENCE REFLECTED LIGHT CUBE LEVER ADJUSTMENT KNOB FINE FOCUS KNOB TRANSMITTED LIGHT ADJUSTMENT KNOB MICROSCOPE BODY COARSE FOCUS KNOB Instruction manual | vwr.com... -
Page 10: It406 Fl4
CONDENSER DIOPTER ADJUSTMENT RING FILTER HOLDER STAGE PLATE STAGE LIGHT PATH SELECTOR LEVER OBJECTIVE X-Y MOVEMENT KNOBS FLUORESCENCE REFLECTED LIGHT CUBE LEVER ADJUSTMENT KNOB FINE FOCUS KNOB TRANSMITTED LIGHT ADJUSTMENT KNOB MICROSCOPE BODY COARSE FOCUS KNOB vwr.com | Instruction manual... -
Page 11: It406 Fl4D
USB CAMERA EYEPIECE DIOPTER ADJUSTMENT RING STAGE PLATE STAGE LIGHT PATH SELECTOR LEVER OBJECTIVE X-Y MOVEMENT FLUORESCENCE KNOBS CUBE LEVER REFLECTED LIGHT ADJUSTMENT KNOB FINE FOCUS KNOB TRANSMITTED LIGHT ADJUSTMENT KNOB MICROSCOPE BODY COARSE FOCUS KNOB Instruction manual | vwr.com... - Page 12 IT406 FL4D (back side) MONITOR POWER SUPPLY HDMI PLUG CONNECTOR MONITOR POWER POWER SUPPLY SUPPLY CABLE CABLE USB MOUSE / KEYBOARD RECEIVER PC STICK USB CABLE (TO CAMERA) PC STICK POWER SUPPLY CABLE HDMI CABLE (TO MONITOR) vwr.com | Instruction manual...
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Page 13: Installation
Mechanical stage can only be installed on the right side of the stage. Installing the units: screw the bolts in the fixing holes of the attachments, then mount the unit from below the stage. (Fig. 4) FIGURE 4 Instruction manual | vwr.com... -
Page 14: Installing The Stage Insert
“AS” mark of filter holder facing front. Align the screw of the condenser illumination unit and the hole of the holder, then screw the bolt in the hole with the supplied Allen wrench. (Fig. 8) FIGURE 8 vwr.com | Instruction manual... -
Page 15: Installing The Led Housing
If the color filter is tilted or otherwise out of place (2), it may fall. FIGURE 11 Color filters can be stacked in the holder. This allows to install as many filters as needed, as long as the whole thickness is less than 11 mm. FIGURE 12 Instruction manual | vwr.com... -
Page 16: Installing The Pc Screen (It406 Fl4D)
Plug the PC stick power supply cable in the slot “C”. Plug the HDMI cable in the slot “D” of the PC stick. (Fig. 15) FIGURE 15 Plug the microscope LED power supply in the connector “E” of the microscope illuminator. (Fig. 16) FIGURE 16 vwr.com | Instruction manual... -
Page 17: Connecting The Power Supply
Plug the power supply into the mains socket. Check for a safe connection. Please use the provided power supply. If lost or damaged, please refer to qualified service. Connect the power supply to a grounded (earthed) wall outlet only. FIGURE 19 Instruction manual | vwr.com... -
Page 18: Brightfield Observation (Transmitted Light)
Adjust interpupillary distance Diopter adjustment ring Adjust diopter Adjust aperture diaphragm Aperture diaphragm Nosepiece Insert in the light path the desired objective and focus the Coarse and fine focusing knobs specimen Brightness adjustment knob Adjust brightness Begin observation vwr.com | Instruction manual... -
Page 19: Use Of The Microscope In Brightfield (Transmitted Light)
Look into the left eyepiece with your left eye only. If the image is not sharp, use the diopter adjustment ring (6) to compensate. (Fig. 23) The adjustment range is ±5 diopter. The number indicated on the adjustment ring graduation should correspond to the operator’s diopter correction. FIGURE 23 Instruction manual | vwr.com... -
Page 20: Adjusting Interpupillary Distance
(Fig. 27) Light path lever Brightness 0% binocular observation / Microphotography or video 100% video or photography acquisition 100% binocular observation / 0% video or photography Binocular observation FIGURE 27 vwr.com | Instruction manual... -
Page 21: Stage And Stage Inserts
Holder for slide and Petri diameter 54 mm (provided with the microscope) Holder for 2+2 slides Holder for Utermöhl-Chamber (holder for Petri diameter 54 mm needed) Load-bearing side extension 10.8.1 Installing stage inserts Install the holder in the mechanical stage. (Fig. 29) FIGURE 29 Instruction manual | vwr.com... -
Page 22: Aperture Diaphragm
32. 70-80% APERTURE 30-20% DIAPHRAGM FIELD OF VIEW FIGURE 32 10.10 Using color filters Selecting the appropriate color filter according your need. (Fig. 33) Filter Green (IF550) Phase contrast microscopy FIGURE 33 vwr.com | Instruction manual... -
Page 23: Use Of The Microscope In Phase Contrast
Place a specimen on the stage and focus it. Take out the eyepiece from the eyepiece tube without the diopter adjustment, and replace it with the centering telescope (CT). (Fig. 36) FIGURE 36 Instruction manual | vwr.com 23... - Page 24 If the phase ring is incorrectly centered, the contrast will be severely impaired. The phase ring may need recentering during and after observation of very thick specimens. The phase ring may show an apparent misalignment if the specimen is not flat. FIGURE 38 FIGURE 39 vwr.com | Instruction manual...
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Page 25: Fluorescence Observation (Reflected Light)
495 nm 520LP nm – Acridine orange: DNA, RNA – Auramine – Rhodamine, TRITC: fluorescent antibodies 540-580 nm 585 nm 590LP nm – Propidium iodide: DNA, RNA – FIGURE 41 Instruction manual | vwr.com 25... -
Page 26: Installing Fluorescence Filter
(1). (Fig. 42) Remove the filter slider from the microscope and put it on a table. Using the screws provided with the fluorescence cube, fix the new cube on the slider. (Fig. 43) FIGURE 42 FIGURE 43 vwr.com | Instruction manual... -
Page 27: Use Of The Anti-Glow Cap
Adjust the light intensity of the reflected light. To obtain the proper observation of the sample, adjust the light intensity of the transmitted light to modulate the intensity of the fluorescence with the one of the phase contrast. Instruction manual | vwr.com... -
Page 28: Microphotography
To calculate the magnification of the camera: objective magnification * camera magnification * lens magnification. If using an SLR camera, mirror movement may cause the camera to vibrate. We suggest lifting the mirror, using long exposure times and a remote cord. vwr.com | Instruction manual... -
Page 29: Troubleshooting
The edge of the image is unfocussed objectives aperture diaphragm Stray light is entering the microscope through the eyepieces Bright patches appear on the image Cover the eyepieces and the viewfinder with a dark cloth and through the camera viewfinder Instruction manual | vwr.com 29... -
Page 30: Repair And Maintenance
Product in its local environment. Note: ethanol and ether are highly flammable liquids. Do VWR will not be held liable for any related omission or for not not use them near a heat source, near sparks or near electric equipment. -
Page 31: Disposal
By doing so, you will help to conserve natural and environmental resources and you will ensure that your equipment is recycled in a manner that protects human health. Thank you Instruction manual | vwr.com... - Page 32 (international) info.no@vwr.com Leicestershire info.fr@vwr.com LE17 4XN + prix appel Tel.: +44 (0) 800 22 33 44 * 0,18 € TTC/min uksales@vwr.com GO TO VWR.COM FOR THE LATEST NEWS, SPECIAL OFFERS AND DETAILS FROM YOUR LOCAL VWR SUPPORT TEAM ZPROVWRI31790-EN ©2021...
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