Trace2O AquaSafe WSL25 Plus Instruction Manual
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Trace2O AquaSafe WSL25 Plus Instruction Manual

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WSL25 Plus
Water Safety
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Instruction Manual

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Summary of Contents for Trace2O AquaSafe WSL25 Plus

  • Page 1 AquaSafe WSL25 Plus Water Safety Laboratory Instruction Manual...
  • Page 2 Contents Section 1 : Introduction Section 2 : Kit Contents Section 3 : WSL25 Incubator Instructions Section 4 : Membrane Filtration Instructions Section 5 : Turbidity Tube Instructions Section 6 : Comparator Instructions Section 7 : pH Pocket Tester Instructions Section 8 : EC Pocket Tester Instructions...
  • Page 3 Introduction The Aquasafe WSL25 Plus is a fully portable field laboratory for the detection of microbiological and physico-chemical water quality parameters. The lab is housed in a waterproof wheeled carry case with telescopic handle and comprises the following : ...
  • Page 4 Kit Contents 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 4, 5, 6, 7 Fig 1.0 Main Case components Fig 1.1 Tray components...
  • Page 5 Main Case Components Item Description Turbidity Tube 5-500NTU Digital Incubator Sampling Cable with Carabiner Multi-Purpose Screwdriver Digital Thermometer Vacuum Pump Lauryl Sulphate Broth Sachet Silicone grease Calibration Pack Vehicle Charging Cable Sample Collection Bag (with dechlorination tablet Box Membrane Filters (100 Pack) Phenol Red reagents (50 Pack) DPD No.1 reagents (50 Pack) DPD No.3 reagents (50 Pack)
  • Page 7 Section 3...
  • Page 9 AquaSafe MSI25 Microbiological Safety Incubator Instruction Manual...
  • Page 10 1.0 Introduction The Aquasafe MSI25 is a portable incubator for the incubation of microbiological samples prepared using the membrane filtration method. The incubator is primarily designed to be used with the supplied 54mm x 3.5mm aluminium petri dishes which are suitable for 47mm membrane filter pads, but the incubator can also be used with 55mm pre-prepared plastic petri-dishes.
  • Page 11 2.0 Incubator set-up The incubator is integrated into the waterproof carry case with a rechargeable battery and power supply. The power pack is comprised of a 12Volt 12Ah Sealed Non-Spillable Lead acid battery. The battery must be electrically isolated during transport on-board aircraft so to facilitate this the power supply is disconnected from the battery by removal of the positive(red) terminal connection and the DC Jack is removed from the incubator socket and fitted with a cap.
  • Page 12 3.0 Operating instructions 3.1 Incubator controls LCD Display Petri-Lok cassette Incubator controls The incubator has an on/off switch and power connector on the right hand side. The on/off switch is pressed in to turn on and depressed to turn off. The incubator can be run from a power supply from 12 –...
  • Page 13 3.2 Getting started It is assumed that the user is familiar with the preparation of samples using the membrane filtration method and as such this manual does not cover this however, instructions on membrane filtration are available on request. The user should familiarise themselves fully with the set-up, control and calibration of the incubator prior to carrying out tests for the first time.
  • Page 14 3.3 Menu sequence Select Option Restart NewRun Fig 1.0 20.9°C 24:00 20.9°C 24:00 10.5v <ADJ 10.5v Fig 1.1 Fig 1.8 20.9°C 24:00 20.9°C 24:00 ->37°C 10.5v <ADJ 10.5v Fig 1.2 Fig 1.7 20.9°C 24:00 20.9°C 24:00 <ADJ 10.5v ->44°C 10.5v Fig 1.6 Fig 1.3 20.9°C...
  • Page 15 3.3.1 Start screen (Figure 1.1) Current temp Time remaining 20.9°C 24:00 10.5v Battery voltage Incubator status Mode This screen shows the current status of the incubator. In the state shown the incubator is in idle mode and not running. 3.3.2 37°C Program (Fig 1.2) 20.9°C 24:00...
  • Page 16 3.3.3 44°C Program (Fig1.3) 20.9°C 24:00 ->44°C 10.5v This mode is the pre-programmed default 44°C incubation cycle. In this mode the incubator will run an 18 hour incubation cycle at 44°C. The 18 hour timer can be altered to any setting between 0 and 24 hours.
  • Page 17 3.3.5 Current Run status (Fig 1.5) 20.9°C 24:00 <SEL 10.5v In this mode pressing the middle switch will display the following screen which will flash intermittently : 15:45 TIMER A SAVE EXIT The time remaining is displayed in the top left corner. This can be reset to the programmed default cycle time by pressing the left switch.
  • Page 18 3.3.7 User defined temperature setting (Fig1.7) 20.9°C 24:00 <ADJ 10.5v In this mode the user defined incubation temperature can be adjusted to a temperature between 20°C and 50°C. To adjust the temperature press the middle switch. The screen will change to : ##.#°C User Temp Save...
  • Page 19 In this mode the incubator is calibrated. To calibrate the unit the following equipment is required: Digital thermometer with 100mm x 4mm stainless steel probe (Not included) Trace2o Incubator calibration pack (Included) Petri-Lok petri-dish cassette. 10 Aluminium Petri dishes. Place 10 empty Petri dishes into the bottom of the cassette.
  • Page 20 5.0 Guarantee and Assistance Trace2o hope that the Aquasafe incubator will give many years of trouble free operation but in the event of a technical problem occurring the Aquasafe incubator is covered by Trace2o Ltds standard Guarantee terms and conditions available via email or via download from www.trace2o.com.
  • Page 21 Section 4...
  • Page 23 AquaSafe Membrane Filtration Instruction Manual...

  • Page 24: Table Of Contents

    Contents Contents SECTION 1: ASSEMBLY OF FILTRATION APPARATUS SECTION 2: PREPARING BACTERIOLOGICAL MEDIA IN A LABORATORY FACILITY SECTION 3: PREPARING BACTERIOLOGICAL MEDIA IN THE FIELD SECTION 4: SAMPLING SECTION 5: USE OF BACTERIOLOGICAL MEDIA SECTION 6: ASEPTIC PROCEDURES SECTION 7: PROCESSING SAMPLES FOR COLIFORM ANALYSIS SECTION 8: COUNTING COLIFORMS AND RECORDING THE RESULT SECTION 9: SELECTING THE OPTIMUM VOLUMES FOR MEMBRANE FILTRATION...

  • Page 25: Section 1: Assembly Of Filtration Apparatus

    SECTION 1: ASSEMBLY OF FILTRATION APPARATUS 1. Hand Vacuum Pump 2. Filtrate Flask / Waste Beaker 3. Sampling Cup and cable 4. Graduated Aluminium Funnel 5. Membrane Support & Holder 6. Sealing Gaskets 7. Glass Sintered Disc (Membrane Support)
  • Page 26 Place one gasket within the recess of the support holder and press into place. Place the sintered disc, smooth side upmost, into the centre of the gasket. Push the other two gaskets into place around the sintered support disc. The gaskets, disc and membrane holder The graduated funnel screws clockwise into position.

  • Page 27: Section 2: Preparing Bacteriological Media In A Laboratory Facility

    SECTION 2: PREPARING BACTERIOLOGICAL MEDIA IN A LABORATORY FACILITY Membrane Lauryl Sulfate Broth: For 50 tests, dissolve 7.62g of Membrane Lauryl Sulfate Broth (one sachet) in 100mL deionised water. The broth is supplied in a pre-weighed sterile sachet, with indicating silica gel that will turn from orange to green, to indicate moisture penetration.

  • Page 28: Section 3: Preparing Bacteriological Media In The Field

    SECTION 3: PREPARING BACTERIOLOGICAL MEDIA IN THE FIELD Membrane Lauryl Sulfate Broth: For 50 tests, dissolve 7.62g of Membrane Lauryl Sulfate Broth (one sachet) in 100mL deionised water. The broth is supplied in a pre-weighed sterile sachet, with indicating silica gel that will turn from orange to green, to indicate moisture penetration.

  • Page 29: Section 4: Sampling

    SECTION 4: SAMPLING Rivers and streams Take the sample as near as possible to the fastest flow – this will typically be found towards the centre of the body of water. Avoid taking samples from too close to the bank, where the water may be still and unrepresentative.
  • Page 30 Heat Disinfection of Tap This method is applicable for metal taps, but not for any plastic taps or taps with non- removable anti-splash devices. Turn off the tap fully, and flame the closed tap with a small Propane or Butane burner; cease flaming if/when any steam issues from the tap.

  • Page 31: Section 5: Use Of Bacteriological Media

    SECTION 5: USE OF BACTERIOLOGICAL MEDIA If stored correctly, the dissolved media should remain stable for 6-8 weeks. However, if there are any signs of contamination e.g. yellowing, cloudiness etc., discard. Ideally, to reduce the possibility of contamination, use one bottle of media only for a 24 hour period, and use a fresh bottle on each subsequent day.

  • Page 32: Section 6: Aseptic Procedures

    SECTION 6: ASEPTIC PROCEDURES Aseptic procedures are of paramount importance during microbiological analysis, and extra care must be taken when outside the central laboratory, i.e. in the field. Everything must be kept clean and sterile, particularly on the following surfaces: Inner surface of the sampling cup Inner surface of the graduated filter funnel Filter membrane and absorbent pads...
  • Page 33 Pads are supplied sterile, in cartridges of 100. A sterile pad dispenser is supplied for depositing the pads into the petri-dishes. It is preferable to dispense pads at the central laboratory, prior to going to the sampling point; in this way, the dispenser may be kept attached to a pad cartridge and remain clean and sterile.

  • Page 34: Section 7: Processing Samples For Coliform Analysis

    SECTION 7: PROCESSING SAMPLES FOR COLIFORM ANALYSIS All samples must be incubated within 6 hours of sampling. Dispense a sterile absorbent pad into a sterile petri dish, and saturate the pad with prepared broth Loosen the graduated filter funnel, and remove from the base support. Sterilise the forceps and allow to cool.
  • Page 35 Pour the water sample into the filter funnel up to the 100 ml graduation. Pour water sample up to 100 ml graduation Connect the hand vacuum pump to the filtration unit base (ensuring the arrow is orientated to the direction of desired air flow) and pump in a controlled fashion to suck the water sample through the membrane.
  • Page 36 When all the water has been filtered, release the vacuum pump and use the sterile forceps to take the membrane from the filtration unit. Use the sterile forceps to take the membrane from the filtration unit Place the membrane on top of the pad, which has been previously saturated with the MLSB media.
  • Page 37 It is important to note that when the last sample has been processed, a resuscitation period of at least one hour (but not more than four hours) must be observed before incubating. This allows any physiologically stressed coliforms to recover before culturing. To incubate faecal (thermotolerant) coliforms, temperature of 44.5°C should be used.

  • Page 38: Section 8: Counting Coliforms And Recording The Result

    SECTION 8: COUNTING COLIFORMS AND RECORDING THE RESULT Note the temperature that the incubator has been set for. Following incubation, switch off the power and remove the petri dishes from the incubator. Place the petri dishes on a flat, level surface. Remove the lids and count all the yellow colonies, irrespective of size.

  • Page 39: Section 9: Selecting The Optimum Volumes For Membrane Filtration

    SECTION 9: SELECTING THE OPTIMUM VOLUMES FOR MEMBRANE FILTRATION The optimum volume of sample is that which will allow the most accurate quantification of bacterial colonies. This is achieved when the number of faecal (thermotolerant) coliform colonies on the membrane following incubation is between 20 and 200 colonies. If there are fewer than 10 colonies, then there exists the possibility of statistical error.
  • Page 41 Section 5...
  • Page 43 AquaSafe Turbidity Tube Instruction Manual...
  • Page 44 SECTION 1: ASSEMBLY OF TURBIDITY TUBE Carefully remove the two halves of the turbidity tube from their position, in the foam recess at the front of the AquaSafe Kit. Align the two halves of the turbidity tube so that the graduations are easily visible, then push together the grey bolting mechanism and screw together using the grey waterproof connector.
  • Page 45 Look through the open end of the tube, at the black square on the base of the tube. This is the Trace2o Secchi marker. Hold the tube vertically, and slowly pour the water sample to be analysed into the tube, until the moment that the Secchi marker is no longer visible from the top of the tube.
  • Page 47 Section 6...
  • Page 49 AquaSafe Comparator Instruction Manual for WSL Range...
  • Page 50 SECTION 1: ASSEMBLY OF COMPARATOR Insert the comparator disc into the recess on the right side of the comparator. SECTION 2: pH ANALYSIS: Fill two cells with 10mL of sample. Place one in the left side of the comparator. This is the blank cell.
  • Page 51 To the other cell add one Phenol Red Rapid Tablet. Crush, cap the cell and invert to mix. This is the test cell. Place the test cell in the right side of the comparator.
  • Page 52 Rotate the disc until colour match is obtained. Record disc reading. pH value = disc reading Notes: pH values below 6.5 always produce a yellow colouration pH values above 8.4 always produce a red colouration Water samples with low total alkalinity may give incorrect results Turbid samples should be filtered prior to analysis for best colour match...
  • Page 53 SECTION 3: CHLORINE ANALYSIS: Fill two cells with 10mL of sample. Place one in the left side of the comparator. This is the blank cell. To the other cell add one DPD No. 1 Tablet. Crush, cap the cell and invert to mix. This is the test cell.
  • Page 54 Place the test cell in the right side of the comparator. Rotate the disc until colour match is obtained. Record disc reading of Free Chlorine.
  • Page 55 Having completed test for free chlorine - Remove cell from right side of comparator, remove lid and add one DPD No. 3 Tablet. Crush, cap the cell and invert to mix., place the test cell in the right side of the comparator.
  • Page 56 Combined Chlorine mg/L (ppm) = Total Chlorine mg/L – Free Chlorine mg/L Notes: Take care not to shake or aerate the sample Store the reagents in a cool, dry place Turbid samples should be filtered prior to analysis for best colour match...
  • Page 57 1.0 Fill the two vials to the 10ml mark with sample water Place one in the left side of the comparator as shown in fig 1.1. This is the reference cell Trace2o Ltd Rev1.0...
  • Page 58 Place the test cell in the right side of the comparator as shown in fig 1.2 Rotate the disc until colour match is obtained. Record disc reading that is displayed in the small viewing window to the bottom right. Ammonia concentration is the reading on the disc. Trace2o Ltd Rev1.0...
  • Page 59 The temperature is critical for full sample development. Below 20°C, increase reaction time to 15 minutes.  The tablets are unsuitable for determination of ammonia in sea water or brackish water due to precipitation of salts.  Turbid samples should be filtered prior to analysis. Trace2o Ltd Rev1.0...
  • Page 60 Trace2o Ltd Rev1.0...
  • Page 61 1.0 Fill the two vials to the 10ml mark with sample water Place one in the left side of the comparator as shown in fig 1.1. This is the reference cell Trace2o Ltd Rev1.0...
  • Page 62 Place the test cell in the right side of the comparator as shown in fig 1.2 Rotate the disc until colour match is obtained. Record disc reading that is displayed in the small viewing window to the bottom right. Nitrate concentration is the reading on the disc. Trace2o Ltd Rev1.0...
  • Page 63 10x and retest.  Interferences may occur from the presence of the following ions : Antinomy (III), Iron (III), Lead, Mercury (I), Silver, Chloroplatinate, Metavanadate, Bismuth.  Copper(II) ions may artificially lower the result. Trace2o Ltd Rev1.0...
  • Page 64 Trace2o Ltd Rev1.0...
  • Page 65 1.0 Fill the two vials to the 10ml mark with sample water Place one in the left side of the comparator as shown in fig 1.1. This is the reference cell Trace2o Ltd Rev1.0...
  • Page 66 Place the test cell in the right side of the comparator as shown in fig 1.2 Rotate the disc until colour match is obtained. Record disc reading that is displayed in the small viewing window to the bottom right. Nitrite concentration is the reading on the disc. Trace2o Ltd Rev1.0...
  • Page 67 The tablets should be added direct from the foil avoiding contact with hands or surfaces.  Interferences may occur from the presence of the following ions : Antinomy (III), Iron (III), Lead, Mercury (I), Silver, Chloroplatinate, Metavanadate, Bismuth.  Copper(II) ions may artificially lower the result. Trace2o Ltd Rev1.0...
  • Page 68 Trace2o Ltd Rev1.0...
  • Page 69 Section 7...
  • Page 71 HydroLite® HL101 Pocket pH Tester Instruction Manual...
  • Page 72 Introduction: The HydroLite® HL101 is a pocket-sized pH and temperature tester. This manual provides instructions on use, with a step-by-step operating guide, as well as care and maintenance instructions. Components: ● HydroLite® HL101 pocket pH/temperature tester ● pH Buffer Solutions (pH4.01/7.00) Keypad: The HL101 pocket pH/temperature tester uses a simple three-button membrane keypad, with graphical symbols to describe the function of each key.
  • Page 73 Display: The unit is equipped with a clear LCD display that is used to show measured value, units, mode indicators and function indicators. Map of mode indicators and descriptions: Mode indicator Description MEAS Indicates unit is in the measurement mode Indicates unit is in the calibration mode SETUP Indicates unit is in SETUP mode...
  • Page 74 Power On/Off: ● Press ON/OFF key to turn on the unit, the display shows measured value. ● Press and hold the ON/OFF key for 5 seconds, the unit will turn off. ● If no key is pressed for 8 minutes, the unit will automatically turn off to conserve power. Note: The auto-off function can be disabled if required –...
  • Page 75 Changing the default parameters: 1. Press and hold the CAL key for 3 seconds to enter the setup menu; the unit goes to buffer standard selection mode, the display shows “USA/BUF” (USA standard). 2. Press CAL key to select the USA or NIST standard for pH buffers. Press ENTER key to confirm;...
  • Page 76 7. Press CAL key to set temperature value. Press ENTER key to confirm; the display shows “YES/HOLD” indicating that the auto-hold function is enabled. Enter If the auto-hold function is enabled, the unit will automatically sense a stable endpoint reading and freeze the reading in the display. If you disable this function, the unit allows user to freeze the reading in the display manually by pressing the HOLD key.
  • Page 77 pH Calibration: The HL101 pocket pH/temperature tester allows up to 3 point calibration. We recommend that you perform at least a 2 point calibration for best accuracy. The unit automatically recognises and calibrates to the following standard buffer values. ● USA Standard Buffer Options: pH 4.01, 7.00, 10.01 ●...
  • Page 78 Rinse the pH electrode with distilled water. Immerse the electrode in the pH 4.01 buffer solution. Stir the solution gently. Press ENTER key to confirm. Wait for the measured value to stabilize; the display shows electrode slope and “END”. 2 point calibration is completed. Enter 3 POINT CALIBRATION (not required for normal operation): Ensure that 3 point calibration is selected in the setup menu.
  • Page 79 Temperature Calibration: During the measurement, if the temperature reading displayed differs from that of an accurate thermometer, you need to calibrate the unit. 1. Press and hold the CAL key for 3 seconds to enter setup menu. 2. Press ENTER key until unit shows the “ C/CAL”...
  • Page 80 Electrode Care and Maintenance: ● For best results, always keep the pH electrode membrane wet. ● Ensure that the electrode is thoroughly washed with distilled water after each use. ● During extended periods of disuse, store the sensor with electrode storage solution. DO NOT use deionised or distilled water.
  • Page 81 Troubleshooting LCD DISPLAY CAUSE CORRECTIVE ACTION Soak the electrode in 3M KCL solution or tap Electrode dried out water for 10 minutes Measured value is out Check whether the electrode membrane is of range clogged, dirty or broken Incorrect buffer Use fresh pH buffer solutions for calibration solutions Electrode is broken...
  • Page 82 Thatcham, Berkshire, RG19 4HZ Tel.: +44 (0)1635 566772 Fax: +44 (0)1635 873509 sales@Trace2o.com www.Trace2o.com The information in this document is subject to change without notice. Copyright © Trace2o® Limited, 2016. All rights reserved. Trace2o® and HydroLite® are registered trademarks of Trace2o® Ltd.
  • Page 83 Section 8...
  • Page 85 HydroLite® HL102 Pocket EC Tester Instruction Manual...
  • Page 86 Introduction: The HydroLite® HL102 is a pocket-sized conductivity (EC), TDS and temperature tester. This manual provides instructions on use, with a step-by-step operating guide, as well as care and maintenance instructions. Components: ● HydroLite® HL102 pocket EC/TDS/temperature tester ● EC Calibration Solution (1413µS) Keypad: The HL102 pocket EC/TDS/temperature tester uses a simple three-button membrane keypad, with graphical symbols to describe the function of each key.
  • Page 87 Display: The unit is equipped with a clear LCD display that is used to show measured value, mode indicators and function indicators. ppm ppt Map of mode indicators and descriptions: Mode indicator Description MEAS Indicates unit is in the measurement mode Indicates unit is in the calibration mode SETUP Indicates unit is in SETUP mode...
  • Page 88 Power On/Off: ● Press ON/OFF key to turn on the unit, the display shows measured value. ● Press and hold the ON/OFF key for 5 seconds, the unit will turn off. ● If no key is pressed for 8 minutes, the unit will automatically turn off to conserve power. Note: The auto-off function can be disabled if required –...
  • Page 89 SETTING THE DEFAULT PARAMETERS: 1. Press and hold the CAL key for 3 seconds to enter setup menu; the display shows currently selected number of calibration points. 2. Press CAL key to select 1 or 2 or 3 point calibration. Press ENTER key to confirm; the unit goes to TDS factor setting mode, the display shows “0.5/TDS”.
  • Page 90 7. Press CAL key to set temperature value. Press ENTER key to confirm; the display shows “YES/HOLD” indicating that the auto-hold function is enabled. Enter If the auto-hold function is enabled, the unit will automatically sense a stable endpoint reading and freeze the reading in the display. If you disable this function, the unit allows user to freeze the reading in the display manually by pressing the HOLD key.
  • Page 91 Conductivity Calibration The HL102 pocket EC/TDS/temperature tester allows up to 3 point calibration in the conductivity mode. To ensure higher accuracy, we recommend that you perform a 3 point calibration, or select a calibration standard concentration close to the sample value you are measuring.
  • Page 92 1.5 Press CAL key to set each digit, press ENTER key to confirm. When the setting is done, ensure the displayed value matches the chosen calibration standard. Enter 1.6 Press ENTER key to start the calibration. Wait for the reading to stabilise; the display shows “END”.
  • Page 93 3 POINT CALIBRATION: If 3 point calibration is selected in the setup menu, when second calibration point is done, the display will immediately show “CAL3”. The unit prompts to continue with third point calibration. Repeat the above steps until the display shows “END”, the unit returns to measurement mode.
  • Page 94 Switching Measurement Mode: 1. In the conductivity mode, press ENTER key until the display shows “TDS”. The unit enters TDS measurement mode. Enter 2. Press ENTER key, the unit enters the salinity measurement mode. Enter 3. Press ENTER key again, the unit returns to conductivity measurement mode. Enter Measurement: Rinse the conductivity electrode with distilled water.
  • Page 95 Electrode Care and Maintenance ● After each use, the conductivity electrode should be rinsed thoroughly in deionised water. ● If there is a build-up of solids inside the measurement area of the cell, these should be removed very carefully with a cotton bud soaked in solvent, taking care not to touch the metal parts of the inner cell.
  • Page 96 Troubleshooting LCD DISPLAY CAUSE CORRECTIVE ACTION Soak the conductivity electrode in tap Electrode dried out water for 10 minutes Measured value is out of Check the electrode whether clogged, range dirty or broken Using the fresh conductivity standard Incorrect calibration solutions solutions for calibration Setting value does not match Reset the calibration value...
  • Page 97 Calibration Points 1 point Calibration Range Measured value ±10°C Temperature Compensation 0~60°C, 32~140°F Temperature Coefficient 2%/°C Cell Constant Normalization Temperature 25°C Hold Function Manual or Automatic Others Manual or Automatic (8 minutes after Power Off last key pressed) Sensor Type Order Code: HL102ELEC Power Requirements 2×1.5V "AAA"...
  • Page 98 Thatcham, Berkshire, RG19 4HZ Tel.: +44 (0)1635 566772 Fax: +44 (0)1635 873509 sales@Trace2o.com www.Trace2o.com The information in this document is subject to change without notice. Copyright © Trace2o® Limited, 2016. All rights reserved. Trace2o® and HydroLite® are registered trademarks of Trace2o® Ltd.

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