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Trace2O AquaSafe WSL50 Instruction Manual

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AquaSafe
WSL50
Water Safety
Laboratory
Instruction Manual

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Summary of Contents for Trace2O AquaSafe WSL50

  • Page 1 AquaSafe WSL50 Water Safety Laboratory Instruction Manual...
  • Page 2 Contents Section 1: Introduction Section 2: Kit Contents Section 3: Double Incubator Instructions Section 4: Membrane Filtration Instructions Section 5: Turbidity Tube Instructions Section 6: Block Tester Instructions...
  • Page 3 Section 1: Introduction The AquaSafe® WSL50 is a fully portable field laboratory for the detection of microbiological and physico-chemical water quality parameters. The lab is housed in a waterproof wheeled carry case with telescopic handle and comprises the following: • Integrated dual chamber digital incubator complete with all accessories and consumables for carrying out 300 tests for Faecal and Total Coliforms.
  • Page 4 Section 2: Kit Contents Under Accessories Box: 6, 7...
  • Page 5 Main Case Components Item Description Sterilisable Work Surface AquaSafe® Double Incubator PetriLok® Cassette with cap and 25 Aluminium Petri Dishes Accessories Box Black Box Silicone Grease Calibration Pack 5 Metre Sampling Cable with Carabiner Tray Components Item Description Sterile Absorbent Pads (100 Pack) Sample Collection Bottles with Dechlorination Tablets 5ml Sterile syringe 30ml Dropper Bottle for Methanol...
  • Page 6 Black Box Description IEC Mains Cable - UK UK to EU Adapter Pool Tester HP1 Phenol Red Rapid Tablets (100 Pack) HP2 DPD No.1 Rapid Tablets (100 Pack) HP3 DPD No.3 Rapid Tablets (100 Pack) Separate Box Description Sterilised Water for Membrane Lauryl Sulfate Broth Preparation Sterile Membrane Filters Sterile Absorbent Pads 2 Part Turbidity Tube 5-500 NTU...
  • Page 7 Section 3: AquaSafe Micro-biological Incubator Instruction Manual...
  • Page 8 Contents 1.0 Introduction ............................. 9 2.0 Incubator set-up ..........................10 2.1 Docking Station .......................... 10 2.2 Battery Removal ........................11 2.3 Battery charging ......................... 11 2.4 Vehicle Power ........................... 12 2.5 Fuse ............................12 3.0 Operating instructions ........................13 3.1 Incubator controls ........................13 3.2 Getting started ...........................
  • Page 9 1.0 Introduction The AquaSafe® Double Incubator is a portable incubator for the incubation of microbiological samples prepared using the membrane filtration method. The incubator is primarily designed to be used with the supplied 54mm x 3.5mm aluminium petri dishes which are suitable for 47mm membrane filter pads, but the incubator can also be used with 55mm pre-prepared plastic petri- dishes.
  • Page 10 2.0 Incubator set-up The incubator is integrated into the waterproof carry case with a docking station which incorporates the rechargeable battery pack and power supply for the unit. An external charger is also supplied which is connected to the port on the rear of the case. On/Off Charger Switch...
  • Page 11 2.2 Battery Removal It may be desired to remove the batteries or replace them at the end of their life. To replace or remove the batteries first ensure the switch on the rear of the carry case is in the off position then remove the screw on the left side of the docking station.
  • Page 12 (Cable available separately) Note: The vehicle cigarette lighter charging lead is a specialist product and as such only a genuine Trace2o® replacement should be used. Replacements are available from any Trace2o® approved representative. No attempt should be made to charge or power the equipment other than via the approved Trace2o®...
  • Page 13 3.0 Operating instructions 3.1 Incubator controls LCD Display The incubator has a power connection Petri-Lok® cassette socket on the rear of the unit and three switches on the top which control all functions of the incubator. The incubator can be run from a power supply in the range 12 –...
  • Page 14 3.2 Getting started Instructions for membrane filtration are covered in another section of the kit manual. The user should familiarise themselves fully with the set-up, control and calibration of the incubator prior to carrying out tests for the first time. If the incubator is connected to the docking station turn on using the switch on the rear of the carry case.
  • Page 15 3.3 Menu Options The incubator is a menu driven system. In section 3.3.1 and 3.3.2 the menu sequences will be outlined. In section 3.3.3 onwards the detailed instructions for each screen will be described for the left chamber. The operation of the right chamber is exactly the same except that the right hand switch will be used to step through the sequence instead of the left.
  • Page 16 3.3.1 Menu sequence – Left Chamber Once the user has selected Restart or NewRun pressing the left switch will step through the following sequence. Where an arrow is shown to the left of the middle command the middle switch will select this action for the left chamber. The bottom right of the display will show the current state of the right chamber, OFF is shown for illustration purposes only.
  • Page 17 3.3.2 Menu sequence – Right Chamber Once the user has selected Restart or NewRun pressing the right switch will step through the following sequence. Where an arrow is shown to the right of the middle command the middle switch will select this action for the right chamber. The bottom left of the display will show the current state of the left chamber, OFF is shown for illustration purposes only.
  • Page 18 3.3.3 Start screen (Figure 1.1) Left Chamber Right Chamber Temperature Temperature 20.9°C 20.9°C Right Chamber Left Chamber status Status Mode This screen shows the current status of the incubator. In the state shown the incubator is in idle mode and not running in either chamber.
  • Page 19 3.3.4 37°C Program (Fig 1.2) 20.9°C 20.9°C ->37°C This mode is the pre-programmed default 37°C incubation cycle. In this mode the incubator will run an 18 hour incubation cycle at 37°C. The timer can be altered to any setting between 0 and 24 hours.
  • Page 20 3.3.5 44°C Program (Fig1.3) 20.9°C 20.9°C ->44°C This mode is the pre-programmed default 44°C incubation cycle. In this mode the incubator will run a 24 hour incubation cycle at 44°C. The timer can be altered to any setting between 0 and 24 hours.
  • Page 21 3.3.6 User defined Program (Fig 1.4) 20.9°C 20.9°C U->##°C This mode will run an incubation cycle at the user defined temperature for 24 hours. Adjusting the user defined temperature is described in section 3.3.9. The 24 hour timer can be altered to any setting between 0 and 24 hours.
  • Page 22 3.3.7 Resetting the timer (Fig 1.5) 20.9°C 20.9°C <SEL In this mode pressing the middle switch will display the following screen which will flash rapidly to warn the user this will reset the timer and the elapsed time data will be lost: 15:45 TIMER A The time remaining is displayed in the top left corner.
  • Page 23 3.3.9 User defined temperature setting (Fig1.7) 20.9°C 20.9°C U->##° <ADJ In this mode the user defined incubation temperature can be adjusted to a temperature between 20°C and 50°C. To adjust the temperature press the middle switch. The screen will change to: ##.#°C User Temp Save...
  • Page 24 In this mode the incubator is calibrated. To calibrate the unit the following equipment is required: Digital thermometer with 100mm x 4mm stainless steel probe (Included) Trace2o® Incubator calibration pack (Included) Fig 3.1 Petri-Lok® Petri-dish cassette 10 Aluminium Petri dishes...
  • Page 25 4.0 Care and Maintenance 4.1 General The AquaSafe® incubator is designed to require minimal maintenance if used correctly and the instructions herein are adhered to, although from time to time cleaning and basic maintenance will be required as outlined in sections 4.2 and 4.3. If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.
  • Page 26 5.1 and replaced as a pair. Should any fault occur please contact the Trace2o® Technical Team who will be more than happy to advise you. 5.0 Technical Specification 5.1 Docking station:...
  • Page 27 47Kg 6.0 Guarantee and Assistance Trace2o® hope that the AquaSafe® incubator will give many years of trouble free operation, but in the event of a technical problem occurring the AquaSafe® incubator is covered by Trace2o® Ltd’s standard Guarantee terms and conditions available via email or via download from www.trace2o.com.

  • Page 28: Contents

    Section 4 AquaSafe Membrane Filtration Instruction Manual Contents...

  • Page 29: Table Of Contents

    Contents SECTION 1: ASSEMBLY OF FILTRATION APPARATUS SECTION 2: PREPARING BACTERIOLOGICAL MEDIA IN A LABORATORY FACILITY SECTION 3: PREPARING BACTERIOLOGICAL MEDIA IN THE FIELD SECTION 4: SAMPLING SECTION 5: USE OF BACTERIOLOGICAL MEDIA SECTION 6: ASEPTIC PROCEDURES SECTION 7: PROCESSING SAMPLES FOR COLIFORM ANALYSIS SECTION 8: COUNTING COLIFORMS AND RECORDING THE RESULT SECTION 9: SELECTING THE OPTIMUM VOLUMES FOR MEMBRANE FILTRATION...

  • Page 30: Section 1: Assembly Of Filtration Apparatus

    SECTION 1: ASSEMBLY OF FILTRATION APPARATUS 1. Hand Vacuum Pistol Pump 2. Filtrate Flask / Waste Beaker 3. Sampling Cup and cable 4. Graduated Aluminium Funnel 5. Membrane Support & Holder 6. Sealing Gaskets 7. Glass Sintered Disc (Membrane Support)
  • Page 31 Place one gasket within the recess of the support holder and press into place. Place the sintered disc, smooth side facing upwards, into the centre of the gasket. Push the other two gaskets into place around the sintered support disc. The graduated funnel screws clockwise into position.

  • Page 32: Section 2: Preparing Bacteriological Media In A Laboratory Facility

    SECTION 2: PREPARING BACTERIOLOGICAL MEDIA IN A LABORATORY FACILITY Membrane Lauryl Sulfate Broth: For 50 tests, dissolve 7.62g of Membrane Lauryl Sulfate Broth (one sachet) in 100mL deionised water. The broth is supplied in a pre-weighed sterile sachet, with indicating silica gel that will turn from orange to green, to indicate moisture penetration.

  • Page 33: Section 3: Preparing Bacteriological Media In The Field

    SECTION 3: PREPARING BACTERIOLOGICAL MEDIA IN THE FIELD Membrane Lauryl Sulfate Broth: For 50 tests, dissolve 7.62g of Membrane Lauryl Sulfate Broth (one sachet) in 100mL deionised water. The broth is supplied in a pre-weighed sterile sachet, with indicating silica gel that will turn from orange to green, to indicate moisture penetration.

  • Page 34: Section 4: Sampling

    SECTION 4: SAMPLING Rivers and streams Take the sample as near as possible to the fastest flow – this will typically be found towards the centre of the body of water. Avoid taking samples from too close to the bank, where the water may be still and unrepresentative.
  • Page 35 Heat Disinfection of Tap This method is applicable for metal taps, but not for any plastic taps or taps with non- removable anti-splash devices. Turn off the tap fully, and flame the closed tap with a small Propane or Butane burner; cease flaming if/when any steam issues from the tap.

  • Page 36: Section 5: Use Of Bacteriological Media

    SECTION 5: USE OF BACTERIOLOGICAL MEDIA If stored correctly, the dissolved media should remain stable for 6-8 weeks. However, if there are any signs of contamination e.g. yellowing, cloudiness etc., discard. Ideally, to reduce the possibility of contamination, use one bottle of media only for a 24 hour period, and use a fresh bottle on each subsequent day.

  • Page 37: Section 6: Aseptic Procedures

    SECTION 6: ASEPTIC PROCEDURES Aseptic procedures are of paramount importance during microbiological analysis, and extra care must be taken when outside the central laboratory, i.e. in the field. Everything must be kept clean and sterile, particularly on the following surfaces: Inner surface of the sampling cup Inner surface of the graduated filter funnel Filter membrane and absorbent pads...
  • Page 38 Pads are supplied sterile, in cartridges of 100. A sterile pad dispenser is supplied for depositing the pads into the petri-dishes. It is preferable to dispense pads at the central laboratory, prior to going to the sampling point; in this way, the dispenser may be kept attached to a pad cartridge and remain clean and sterile.

  • Page 39: Section 7: Processing Samples For Coliform Analysis

    SECTION 7: PROCESSING SAMPLES FOR COLIFORM ANALYSIS All samples must be incubated within 6 hours of sampling. Dispense a sterile absorbent pad into a sterile petri dish, and saturate the pad with prepared broth Loosen the graduated filter funnel, and remove from the base support. Sterilise the forceps and allow to cool.
  • Page 40 Connect the hand vacuum pump to the filtration unit base and pump in a controlled fashion to suck the water sample through the membrane.
  • Page 41 When all the water has been filtered, release the vacuum pump and use the sterile forceps to take the membrane from the filtration unit. Place the membrane on top of the pad, which has been previously saturated with the MLSB media.

  • Page 42: Section 8: Counting Coliforms And Recording The Result

    SECTION 8: COUNTING COLIFORMS AND RECORDING THE RESULT Note the temperature that the incubator has been set for. Following incubation, switch off the power and remove the petri dishes from the incubator. Place the petri dishes on a flat, level surface. Remove the lids and count all the yellow colonies, irrespective of size.

  • Page 43: Section 9: Selecting The Optimum Volumes For Membrane Filtration

    SECTION 9: SELECTING THE OPTIMUM VOLUMES FOR MEMBRANE FILTRATION The optimum volume of sample is that which will allow the most accurate quantification of bacterial colonies. This is achieved when the number of faecal (thermotolerant) coliform colonies on the membrane following incubation is between 20 and 200 colonies. If there are fewer than 10 colonies, then there exists the possibility of statistical error.
  • Page 44 Section 5: AquaSafe Turbidity Tube Instruction Manual...
  • Page 45 SECTION 1: ASSEMBLY OF TURBIDITY TUBE Carefully remove the two halves of the turbidity tube from their position, in the foam recess at the front of the AquaSafe Kit. Align the two halves of the turbidity tube so that the graduations are easily visible, then push together the grey bolting mechanism and screw together using the grey waterproof connector.
  • Page 46 Look through the open end of the tube, at the black square on the base of the tube. This is the Trace2o Secchi marker. Hold the tube vertically, and slowly pour the water sample to be analysed into the tube, until the moment that the Secchi marker is no longer visible from the top of the tube.
  • Page 47 Section 6: AquaSafe Block Tester Instruction Manual for WSL Range...
  • Page 48 SECTION 1: GETTING TO KNOW YOUR BLOCK TESTER There are five chambers – three behind coloured overlays, and two clear. The two clear chambers are those into which tablets should be added. The chambers are referred to by a number from 1-5, as shown in the above image. SECTION 2: PREPARATION: Remove the black rubber cap and immerse the entire block tester in the water sample to Grip the pool tester by the sides –...
  • Page 49 SECTION 3: pH (PHENOL RED) TABLET Take 1 x Phenol Red tablet directly from the foil, and add to chamber 2. SECTION 4: FREE CHLORINE (DPD1) TABLET...
  • Page 50 Take 1 x DPD1 tablet directly from the foil, and add to chamber 4. Recap the block tester firmly, ensuring that the arrows on the black rubber cap are pointing towards the coloured overlays. Shake vigorously, and hold up to the light to read the result.
  • Page 51 pH - Choose the closest colour match between the colour of the test sample in chamber 2, and the coloured overlay on chamber 1. Record the result. Free Chlorine – Choose the closest colour match between the colour of the test sample in chamber 4, and the coloured overlays on chambers 3 and 5.
  • Page 52 Total Chlorine – Choose the closest colour match between the colour of the test sample in chamber 4, and the coloured overlays on chambers 3 and 5. Record the result. Combined Chlorine mg/L (ppm) = Total Chlorine mg/L – Free Chlorine mg/L Notes: Take care not to over shake or aerate the sample.

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