Aquasafe WSL50 Pro Instruction Manual
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Aquasafe WSL50 Pro Instruction Manual

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Summary of Contents for Aquasafe WSL50 Pro

  • Page 1 AquaSafe WSL50 Pro Water Safety Laboratory Instruction Manual...
  • Page 2 Contents Section 1: Introduction Section 2: Kit Contents Section 3: Double Incubator Instructions Section 4: Membrane Filtration Instructions Section 5: HydroTest HT1000 Instructions Section 6: Multi-parameter Instructions...
  • Page 3 Section 1: Introduction The AquaSafe® WSL50 Pro is a fully portable field laboratory for the detection of microbiological and physico-chemical water quality parameters. The lab is housed in a waterproof wheeled carry case with telescopic handle and comprises the following: ...
  • Page 4 Section 2: Kit Contents...
  • Page 5 Main Case Components Item Description Sterilisable Work Surface AquaSafe® Double Incubator PetriLok® Cassette with cap and 25 Aluminium Petri Dishes Accessories Box HydroTest® HT1000 Bag HydroCheck® and Buffers Bag Silicone Grease Calibration Pack 5 Metre Sampling Cable with Carabiner IEC Mains Cable – UK...
  • Page 6 HT1000 Bag Components Description HydroTest® HT1000 Digital Photometer Photometer 24 mm Sample Vials Crushing Rod Lint-Free Wipes (10 Pack) HydroCheck® and Buffers Bag Components Description HydroCheck® HC1000 Multi-Parameter Electrochemical Meter pH Probe for HydroCheck® EC Probe for HydroCheck® Thermal Probe for HydroCheck® pH 4.01 Buffer Solution pH 7.00 Buffer Solution EC 1413µS Calibration Solution...
  • Page 7 Section...
  • Page 8 AquaSafe Micro-biological Incubator Instruction Manual...

  • Page 9: Table Of Contents

    Contents 1.0 Introduction ......................11 2.0 Incubator set-up ....................13 2.1 Docking Station ....................13 2.2 Battery Removal..................... 14 2.3 Battery charging ..................... 14 2.4 Vehicle Power ....................15 2.5 Fuse ........................ 15 3.0 Operating instructions ..................17 3.1 Incubator controls ..................17 3.2 Getting started ....................
  • Page 10 6.0 Guarantee and Assistance ................... 32...

  • Page 11: Introduction

    1.0 Introduction The AquaSafe® Double Incubator is a portable incubator for the incubation of microbiological samples prepared using the membrane filtration method. The incubator is primarily designed to be used with the supplied 54mm x 3.5mm aluminium petri dishes which are suitable for 47mm membrane filter pads, but the incubator can also be used with 55mm pre-prepared plastic petri-dishes.

  • Page 13: Incubator Set-Up

    2.0 Incubator set-up The incubator is integrated into the waterproof carry case with a docking station which incorporates the rechargeable battery pack and power supply for the unit. An external charger is also supplied which is connected to the port on the rear of the case.

  • Page 14: Battery Removal

    2.2 Battery Removal It may be desired to remove the batteries or replace them at the end of their life. To replace or remove the batteries first ensure the switch on the rear of the carry case is in the off position then remove the screw on the left side of the docking station.

  • Page 15: Vehicle Power

    Note: The battery charger comes complete with an IEC C19 to UK mains lead. Replacement leads must be of the same type with a sealed plug and maximum cable length of 2 metres, and must carry the CE mark. 2.4 Vehicle Power The incubator is also supplied with a vehicle cigarette lighter cable.

  • Page 17: Operating Instructions

    3.0 Operating instructions 3.1 Incubator controls The incubator has a power connection socket on the rear of the unit and three switches on the top which control all functions of the incubator. The incubator can be run from a power supply in the range 12 –...

  • Page 18: Getting Started

    3.2 Getting started Instructions for membrane filtration are covered in another section of the kit manual. The user should familiarise themselves fully with the set-up, control and calibration of the incubator prior to carrying out tests for the first time. If the incubator is connected to the docking station turn on using the switch on the rear of the carry case.

  • Page 19: Menu Options

    3.3 Menu Options The incubator is a menu driven system. In section 3.3.1 and 3.3.2 the menu sequences will be outlined. In section 3.3.3 onwards the detailed instructions for each screen will be described for the left chamber. The operation of the right chamber is exactly the same except that the right hand switch will be used to step through the sequence instead of the left.

  • Page 20: Menu Sequence - Left Chamber

    3.3.1 Menu sequence – Left Chamber Once the user has selected Restart or New Run pressing the left switch will step through the following sequence. Where an arrow is shown to the left of the middle command the middle switch will select this action for the left chamber. The bottom right of the display will show the current state of the right chamber, OFF is shown for illustration purposes only.

  • Page 22: Menu Sequence - Right Chamber

    3.3.2 Menu sequence – Right Chamber Once the user has selected Restart or New Run pressing the right switch will step through the following sequence. Where an arrow is shown to the right of the middle command the middle switch will select this action for the right chamber. The bottom left of the display will show the current state of the left chamber, OFF is shown for illustration purposes only.

  • Page 23: Start Screen (Figure 1.1)

    3.3.3 Start screen (Figure 1.1) 20.9°C 20.9°C This screen shows the current status of the incubator. In the state shown the incubator is in idle mode and not running in either chamber.

  • Page 24: 37°C Program (Fig 1.2)

    3.3.4 37°C Program (Fig 1.2) 20.9°C 20.9°C ->37°C This mode is the pre-programmed default 37°C incubation cycle. In this mode the incubator will run an 18-hour incubation cycle at 37°C. The timer can be altered to any setting between 0 and 24 hours. Adjusting the incubation time is described in section 3.3.8.

  • Page 25: 44°C Program (Fig1.3)

    3.3.5 44°C Program (Fig1.3) 20.9°C 20.9°C ->44°C This mode is the pre-programmed default 44°C incubation cycle. In this mode the incubator will run a 24 hour incubation cycle at 44°C. The timer can be altered to any setting between 0 and 24 hours. Adjusting the incubation time is described in section 3.3.8.

  • Page 26: User Defined Program (Fig 1.4)

    3.3.6 User defined Program (Fig 1.4) 20.9°C 20.9°C U->##°C This mode will run an incubation cycle at the user defined temperature for 24 hours. Adjusting the user defined temperature is described in section 3.3.9. The 24 hour timer can be altered to any setting between 0 and 24 hours. Adjusting the incubation time is described in section 3.3.8.

  • Page 27: Resetting The Timer (Fig 1.5)

    3.3.7 Resetting the timer (Fig 1.5) 20.9°C 20.9°C <SEL In this mode pressing the middle switch will display the following screen which will flash rapidly to warn the user this will reset the timer and the elapsed time data will be lost: 15:45 TIMER A The time remaining is displayed in the top left corner.

  • Page 28: User Defined Temperature Setting (Fig1.7)

    3.3.9 User defined temperature setting (Fig1.7) 20.9°C 20.9°C U->##° <ADJ In this mode the user defined incubation temperature can be adjusted to a temperature between 20°C and 50°C. To adjust the temperature, press the middle switch. The screen will change to: ##.#°C User Temp Save...

  • Page 29: Calibration (Fig 1.8)

    3.3.10 Calibration (Fig 1.8) 20.9°C 20.9°C <ADJ In this mode the incubator is calibrated. To calibrate the unit the following equipment is required: Digital thermometer with 100mm x 4mm stainless steel probe (Included) Trace2o® Incubator calibration pack (Included) Petri-Lok® Petri-dish cassette 10 Aluminium Petri dishes Assemble the cassette as follows: Fig 3.1...

  • Page 30: Care And Maintenance

    The Petri-Lok® rack can be steam sterilised if required but it is not recommended to steam sterilise the top. The AquaSafe® carry case and integral docking station is waterproof when the lid is fully closed and the connector cap is fitted. When closed the case can be washed with a mild soap solution or rinsed with a hose.

  • Page 31: Maintenance

    4.3 Maintenance The wheels should be periodically cleaned. When dry add a drop of lubricating oil to the wheel axles to prevent seizing. All screws in the case are fitted with anti-vibration nuts or secured with adhesive. However, after transporting the case in a vehicle, it is suggested to check the integrity of the docking station and any screws to ensure no damage has occurred and all electrical connections are secure prior to use.

  • Page 32: Incubator

    47Kg 6.0 Guarantee and Assistance Trace2o® hope that the AquaSafe® incubator will give many years of trouble free operation, but in the event of a technical problem occurring the AquaSafe® incubator is covered by Trace2o® Ltd.’s standard Guarantee terms and conditions available via email or via download from www.trace2o.com.
  • Page 33 Section...
  • Page 34 AquaSafe Membrane Filtration Instruction Manual...
  • Page 35 Contents Contents SECTION 1: ASSEMBLY OF FILTRATION APPARATUS SECTION 2: PREPARING BACTERIOLOGICAL MEDIA IN A LABORATORY FACILITY 38 SECTION 3: PREPARING BACTERIOLOGICAL MEDIA IN THE FIELD SECTION 4: SAMPLING SECTION 5: USE OF BACTERIOLOGICAL MEDIA SECTION 6: ASEPTIC PROCEDURES SECTION 7: PROCESSING SAMPLES FOR COLIFORM ANALYSIS SECTION 8: COUNTING COLIFORMS AND RECORDING THE RESULT SECTION 9: SELECTING THE OPTIMUM VOLUMES FOR MEMBRANE FILTRATION 2...

  • Page 36: Section 1: Assembly Of Filtration Apparatus

    SECTION 1: ASSEMBLY OF FILTRATION APPARATUS Hand Vacuum Pistol Pump Filtrate Flask / Waste Beaker Sampling Cup and cable Graduated Aluminium Funnel Membrane Support & Holder Sealing Gaskets Glass Sintered Disc (Membrane Support...
  • Page 37 Place one gasket within the recess of the support holder and press into place. Place the sintered disc, smooth side facing upwards, into the centre of the gasket. Push the other two gaskets into place around the sintered support disc. The graduated funnel screws clockwise into position.

  • Page 38: Section 2: Preparing Bacteriological Media Ina Laboratory Facility

    SECTION 2: PREPARING BACTERIOLOGICAL MEDIA IN A LABORATORY FACILITY Membrane Lauryl Sulfate Broth: For 50 tests, dissolve 7.62g of Membrane Lauryl Sulfate Broth (one sachet) in 100mL deionised water. The broth is supplied in a pre-weighed sterile sachet, with indicating silica gel that will turn from orange to green, to indicate moisture penetration.

  • Page 39: Section 3: Preparing Bacteriological Media In The Field

    SECTION 3: PREPARING BACTERIOLOGICAL MEDIA IN THE FIELD Membrane Lauryl Sulfate Broth: For 50 tests, dissolve 7.62g of Membrane Lauryl Sulfate Broth (one sachet) in 100mL deionised water. The broth is supplied in a pre-weighed sterile sachet, with indicating silica gel that will turn from orange to green, to indicate moisture penetration.

  • Page 40: Section 4: Sampling

    SECTION 4: SAMPLING Rivers and streams Take the sample as near as possible to the fastest flow – this will typically be found towards the centre of the body of water. Avoid taking samples from too close to the bank, where the water may be still and unrepresentative.
  • Page 41 Heat Disinfection of Tap This method is applicable for metal taps, but not for any plastic taps or taps with non-removable anti-splash devices. Turn off the tap fully, and flame the closed tap with a small Propane or Butane burner; cease flaming if/when any steam issues from the tap.

  • Page 42: Section 5: Use Of Bacteriological Media

    SECTION 5: USE OF BACTERIOLOGICAL MEDIA If stored correctly, the dissolved media should remain stable for 6-8 weeks. However, if there are any signs of contamination e.g. yellowing, cloudiness etc., discard. Ideally, to reduce the possibility of contamination, use one bottle of media only for a 24-hour period, and use a fresh bottle on each subsequent day.

  • Page 43: Section 6: Aseptic Procedures

    SECTION 6: ASEPTIC PROCEDURES Aseptic procedures are of paramount importance during microbiological analysis, and extra care must be taken when outside the central laboratory, i.e. in the field. Everything must be kept clean and sterile, particularly on the following surfaces: Inner surface of the sampling cup Inner surface of the graduated filter funnel Filter membrane and absorbent pads...
  • Page 44 Pads are supplied sterile, in cartridges of 100. A sterile pad dispenser is supplied for depositing the pads into the petri-dishes. It is preferable to dispense pads at the central laboratory, prior to going to the sampling point; in this way, the dispenser may be kept attached to a pad cartridge and remain clean and sterile.

  • Page 45: Section 7: Processing Samples For Coliform Analysis

    SECTION 7: PROCESSING SAMPLES FOR COLIFORM ANALYSIS All samples must be incubated within 6 hours of sampling. Dispense a sterile absorbent pad into a sterile petri dish, and saturate the pad with prepared broth Loosen the graduated filter funnel, and remove from the base support. Sterilise the forceps and allow to cool.
  • Page 46 Pour the water sample into the filter funnel up to the 100 ml graduation. Connect the hand vacuum pump to the filtration unit base and pump in a controlled fashion to suck the water sample through the membrane.
  • Page 47 When all the water has been filtered, release the vacuum pump and use the sterile forceps to take the membrane from the filtration unit. Place the membrane on top of the pad, which has been previously saturated with the MLSB media. Replace the petri-dish lid and mark the petri dish.

  • Page 48: Section 8: Counting Coliforms And Recording The Result

    SECTION 8: COUNTING COLIFORMS AND RECORDING THE RESULT Note the temperature that the incubator has been set for. Following incubation, switch off the power and remove the petri dishes from the incubator. Place the petri dishes on a flat, level surface. Remove the lids and count all the yellow colonies, irrespective of size.

  • Page 49: Section 9: Selecting The Optimum Volumes For Membrane Filtration

    SECTION 9: SELECTING THE OPTIMUM VOLUMES FOR MEMBRANE FILTRATION The optimum volume of sample is that which will allow the most accurate quantification of bacterial colonies. This is achieved when the number of faecal (thermotolerant) coliform colonies on the membrane following incubation is between 20 and 200 colonies.
  • Page 50 Section 5 HydroTest Photometer HT1000...
  • Page 51 Contents Introduction ................... 6 Operation ....................7 2.1.1 Set Up ..................7 2.1.2 Saving Data – Important Information ......... 7 2.1.3 Replacement of Batteries............7 2.1.4 Instrument Diagram ..............8 Overview of Function Keys ............... 9 2.2.1 Overview ..................9 2.2.2 Displaying Time and Date............
  • Page 52 2.3.13 Measuring Absorbance ............. 19 Photometer Settings: Table of Mode Functions ......20 2.4.1 Instrument Basic Settings 1 ............22 2.4.2 Printing of Stored Results ............25 2.4.3 Recall/Delete Stored Results ............. 29 2.4.4 Calibration ................34 2.4.5 Lab Function ................44 2.4.6 User Options ................

  • Page 53: Introduction

    1.0 Introduction The HT1000 is part of the HydroTest range of instruments based on optical measurement technology for the analysis of a range of chemical parameters in aqueous samples. The HT1000 is a photometric analyser using 6 individual selective wavelengths to measure the colour change produced by a reagent when reacted with the analyte of interest.

  • Page 54: Operation

    2.0 Operation 2.1.1 Set Up Before working with the photometer, it may be necessary to insert the batteries if supplied separately. See chapters 2.1.2 Saving Data – Important Information and 2.1.3 Replacement of Batteries. Before using the photometer, perform the following settings in the Mode-Menu. This is achieved by turning the device on and pressing the [MODE] key.

  • Page 55: Instrument Diagram

    2.1.4 Instrument Diagram Screws Battery Compartment Cover Notch (D) Batteries – 4 x AA/LR6 Seal Ring Instrument Back CAUTION: To ensure that the instrument is waterproof: Seal ring (E) must be in position Battery compartment cover (B) must be fixed with the four screws...

  • Page 56: Overview Of Function Keys

    Overview of Function Keys 2.2.1 Overview Switching the photometer on or off Press shift key to achieve figures 0-9. Keep the shift key depressed and press desired figured key. E.g. [Shift] + [1] [1] Function 1 key: description in the text if key available Function 2 key: description in the text if key available Function 3 key: description in text if key available Displaying date and time / user countdown...

  • Page 57: Displaying Time And Date

    Performing Zero Decimal point 2.2.2 Displaying Time and Date Press [‘clock’] key. 19:30:22 2012-06-15 Display shows: After 15 seconds, the photo meter reverts to the previous display automatically, alternatively press [ESC] or [ ]. 2.2.3 User Countdown With this function the operator is able to define their own countdown. Press [‘clock’] key.

  • Page 58: Display Backlight

    E.g. 2 minutes 0 seconds = [Shift] + [0] [2] [0] [0] Confirm with [ ] key. Countdown 02:00 The display shows: Start: Start countdown with [ ] key. After countdown has finished, the photometer reverts to the previous display automatically. 2.2.4 Display Backlight Press [Shift] + [F1] keys to turn the display backlight on or off.

  • Page 59: Selecting A Method

    2.3.2 Selecting a Method The display shows a selection: >> 20 Acid demand T 35 Alkalinity-p T There are two possible ways to select the 40 Alkalinity-tot P required method: …. a) Press arrow key [ ] or [ ] to select the desired method from the displayed list.

  • Page 60: Chemical Species Information

    2.3.4 Chemical Species Information Pressing the [F2] key for a method in the method list gives the available chemical species and corresponding ranges. See Chapter 2.3.8 Changing Chemical Species Example: 320 Phosphate LR T Line 1: Method number, method name Line 2: Range with chemical species 1 0.05-4 mg/l PO Line 3: Range with chemical species 2...

  • Page 61: Performing Zero

    2.3.6 Performing Zero The display shows: prepare Zero Prepare a clean vial as describe in the relevant application note and place the vial in the sample press ZERO chamber, ensuring that the triangular markings on the vial and HT1000 are aligned. Press [ZERO] key.

  • Page 62: Performing Tests

    2.3.7 Performing Tests When zero calibration is complete, remove the vial from the sample chamber and perform the tests as described in the relevant application note. When the results have been displayed, options include: Storing and/or printing the results For some methods, changing between different chemical species Performing further analysis with the same zero Selecting a new method 2.3.8 Changing Chemical Species...

  • Page 63: Storing Results

    2.3.9 Storing Results Press [STORE] key while the test result is displayed. The display shows: Code-No.: _ _ _ _ _ _ We advise you to enter a numeric code (up to 6 digits). A code no. can contain references to the operator or the sampling location.

  • Page 64: Printing Results - T2O Pc-Link (Optional)

    2.3.10 Printing Results – T2o PC-Link (Optional) If the PC-Link module is switched on (see Chapter 2.5 Data Transfer) and the printer is connected, it is possible to print out the test results without saving beforehand. Press [F3] key. The entire data set is printed with date, time Code No., method and test result.

  • Page 65: Performing Additional Measurements

    2.3.11 Performing Additional Measurements To perform additional tests using the same method Press [TEST] key. The display shows: Zero accepted prepare Test press TEST Confirm with [TEST] key. Or press [ZERO] key to perform a new zero calibration. The display shows: prepare Zero press ZERO 2.3.12 Selecting a New Method...

  • Page 66: Measuring Absorbance

    2.3.13 Measuring Absorbance Range: - 2600 mAbs to + 2600 mAbs Method-No. Method Title mAbs 430 nm mAbs 530 nm mAbs 560 nm mAbs 580 nm mAbs 610 nm mAbs 660 nm Select the desired wavelength from the method list or by entering the corresponding method number directly.

  • Page 67: Photometer Settings: Table Of Mode Functions

    2.4 Photometer Settings: Table of Mode Functions Mode-Function Description Page Language Select Language Key-beep Switch the acoustic signal off/off to indicate key-pressing Clock Set date and time Countdown Switch countdown on/off to ensure reaction times Signal-beep Switch acoustic signal on/off to indicate end of reading Print Print all stored results Print, date...
  • Page 68 M list all off User method list, switching off all methods User concentr. Enter the data necessary to run a user concentration method User polynoms Enter the data necessary to run a user polynomial User m. clear Delete all data of a user polynomial or concentration method User m.

  • Page 69: Instrument Basic Settings 1

    2.4.1 Instrument Basic Settings 1 Selecting a Language Press [MODE]. [Shift] + [1] [0] keys. Confirm with [ ] key. The display shows: < Language > Deutsch Press arrow key [ ] or [ ] to select the required language from the displayed list. >>...
  • Page 70 Setting Date and Time Press [MODE], [Shift] + [1] [2] keys. Confirm with [ ] key. The display shows: < Clock > The entry comprises two digits each for year, yy-mm-dd hh:mm month day, minutes and seconds. _ _-_ _-_ _ _ _:_ _ Enter year, month and day <...
  • Page 71 Press [Shift] + [0] keys to switch the countdown off. Press [Shift] + [1] keys to switch the countdown on. Confirm with [ ] key. Note: It is possible to interrupt the working countdown by pressing the [ ] key (application e.g.

  • Page 72: Printing Of Stored Results

    2.4.2 Printing of Stored Results Printing All Results Press [MODE], [Shift] + [2] [0] keys. Confirm with [ ] key. The display shows: < Print > print all data Start: cancel: ESC Press [ ] for printing out all stored results. The display shows e.g.
  • Page 73 Printing Results of a Selected Time Period Press [MODE], [Shift] + [2] [1] keys. Confirm with [ ] key. The display shows: < Print > Enter year, month and day for the first day of the sorted: date required period e.g. 14 May 2009 = [Shift] + [0] [9] from yy-mm-dd [0] [5] [1] [4] _ _-_ _-_ _...
  • Page 74 After printing the photometer goes back to mode menu automatically. Note: It is possible to cancel the entry by pressing [ESC]. If you want to print only results of one day, enter the same day twice to determine the period. Printing Results of a Selected Code No.
  • Page 75 Printing Results of a Selected Method Press [MODE], [Shift] + [2] [3] keys. Confirm with [ ] key. The display shows: < Print > Select the required method from the displayed list >> 20 Acid demand T or enter the method number directly. 35 Alkalinity-p T 30 Alkalinity-tot T ….

  • Page 76: Recall/Delete Stored Results

    Printing Parameter Press [MODE], [Shift] + [2] [9] keys. Confirm with [ ] key. The display shows: < Printing params. > 2: Baud rate cancel: ESC Press [Shift] + [2] keys to select “Baud rate”. The display shows: < Baud rate > is: 9600 select ↑↓: save:...
  • Page 77 Confirm with [ ] key. The display shows: < Storage > The stored data sets are displayed in chronological display all data order, starting with the latest stored test results. Start: cancel: ESC Press [ ] key and all stored results are displayed. print: F3 print all: F2 Press [F3] key to print the displayed result.
  • Page 78 Confirm with [ ] key. The display shows: < Storage > Enter year, month and day for the first day of the sorted: date required period, e.g. 19 May 2009 = [Shift] + [0] [9] to yy-mm-dd [0] [5] [1] [9] _ _ - _ _ - _ _ Confirm with [ ] key.
  • Page 79 Recall Results of a Selected Code No. Range Press [MODE], [Shift] + [3] [2] keys. Confirm with [ ] key. The display shows: < Storage > Enter numeric code number (up to 6 places) for the first required Code No. e.g.: [Shift] + [1] sorted: Code-No.
  • Page 80 Recall Results of a Selected Method Press [MODE], [Shift] + [3] [3] keys. Confirm with [ ] key. The display shows: < Storage > Select the required method from the displayed list >> 20 Acid demand T or enter the method number directly. 35 Alkalinity-p T 30 Alkalinity-tot T ….

  • Page 81: Calibration

    Delete Stored Results Press [MODE], [Shift] + [3] [4] keys. Confirm with [ ] key. The display shows: < Delete data > Select the required method from the displayed list Delete all data? or enter the method number directly. YES: 1 NO: 0 Press [Shift] + [0] keys to retain the data sets in memory.
  • Page 82 The display shows: < Calibration > 1: M191 Ca-hardness 2 2: M191 reset 0 cali. 3: M170 Fluoride L Press [Shift] + [1] keys. The display shows: < Calibration > 1. Fill a clean vial (24 mm Ø) with exactly 10 ml of deionised water, close tightly with the cap.
  • Page 83 10. Press [TEST] key. The batch related method blank is saved. Press [ ] key to go back to mode menu. Notes: 1. If a new batch of CALCIO tablets is used, a calibration of the method blank has to be performed to optimise the results.
  • Page 84 Press [Shift] + [1] keys to erase the method blank and set the value back to factory calibration. Fluoride Method 170 Press [MODE], [Shift] + [4] [0] keys. Confirm with [ ] key. The display shows: < Calibration > 1: M191 Ca-hardness 2 2: M191 reset 0 cali.
  • Page 85 The display shows: < Calibration > 9. Remove the vial from the sample chamber, empty the vial, rinse the vial and cap several times 170 Fluoride L and the fill the vial with exactly 10 ml Fluoride T1 accepted standard (concentration 1 mg/l F). 10.
  • Page 87 User Calibration If a test method is user calibrated, the method name is displayed inverse. Procedure: Prepare a standard of known concentration and use this standard instead of the sample according to the test procedure. It is recommended to use well known standard which hare formulated according to DIN EN, ASTM or other international norms or to use the certified standards which are commercially available.
  • Page 88 Cyanide 0.1-0.3 mg/l CN Fluoride Calibration with 0 and 1 mg/l F through mode 40 Calibration with basic test 100 chlorine free Method Recommended range for user calibration Hardness, Calcium 100-200 mg/l CaCO Hardness, Calcium 2 100-200 mg/l CaCO Hardness-total 15-25 mg/l CaCO Iodine Calibration with basic test 100 chlorine free...
  • Page 89 Store User Calibration Perform the required method as described in the manual using a standard of known concentration instead of 100 Chlorine T the water sample. 0.02-6 mg/l Cl2 0.90 mg/l free Cl2 If the test result is displayed, press [MODE], [Shift] + [4] [5] keys. Confirm with [ ] key.
  • Page 90 Now the method name is displayed inverse and the Chlorine T test result is calculated with new calibration factor. 0.02-6 mg/l Cl2 1.00 mg/l free Cl2 Delete User Calibration This chapter only applies for the methods which can be user calibrated. Select the required method.

  • Page 91: Lab Function

    2.4.5 Lab Function Reduced Operator Guidance – ‘Profi-Mode’ This function may be used for routine analyses with many samples of one method. The following information is always stored in the methods: a) Method b) Range c) Date and time d) Differentiation of results e) Detailed operator instruction f) Compliance with reaction periods If the Profi-Mode is active, the photometer provides only a minimum of operator...
  • Page 92 The selected settings are kept by the photometer even when it is switched off. To change the photometer settings, a new setting is required. One Time Zero (OTZ) One Time Zero is available for all methods where Zero is performed in a 24 mm Ø round vial with sample water.

  • Page 93: User Options

    Note: The specified accuracy is valid for all test results when Zero is performed for each test (One Time Zero function is switched off). 2.4.6 User Options User Method List After switching on the instrument, a scroll list of all available methods is automatically shown in the display.
  • Page 94 Switch with [F2] key between ‘active’ [•] and ‘inactive’ [ ]. Select next method, activate or inactivate as required and repeat. Confirm with [ ] key. Cancel without storing by pressing [ESC] key. Recommendation: If only a few methods are required, it is recommended to perform Mode 62 first, followed by Mode 60.
  • Page 95 User Method List, Switch All Methods On This mode function activates all methods. After switching on the instrument a scroll list of all available methods is automatically shown in the display. Press [MODE], [Shift] + [6] [1] keys. Confirm with [ ] key. The display shows: <Mlist all on>...
  • Page 96 Press [Shift] + [0] keys to keep the valid method selection. The instrument goes back to mode menu automatically. User Concentration Methods It is possible to enter and store up to 10 User Concentration Methods. Therefore, you need 2 to 14 standards of know concentration and one blank (deionised water or reagent blank value).
  • Page 97 Press [Shift] + [0] or [ESC] keys to go back to method no. query. Press [Shift] + [1] keys to start entry mode. The display shows: <User concentr.> wavelength: 1: 530 nm 4: 430 nm 2: 560 nm 5: 580 nm 3:610 nm 6: 660 nm Enter the required wavelength e.g.
  • Page 98 0.000 … 9.999 0.001 10.00 … 99.99 0.01 100.0 … 999.9 1000 … 9999 MEASUREMENT PROCEDURE WITH STANDARDS OF KNOWN CONCENTRATION The display shows: <User concentr.> prepare Zero press ZERO Prepare Zero and press [ZERO] key. Note: Use deionised water or reagent blank value. The display shows: <User concentr.>...
  • Page 99 The display shows the input value and measured <User concentr.> absorption value. S1: 0.05 mg/l mAbs: 12 Confirm with [ ] key. The display shows: <User concentr.> S1 accepted S2: + _____ │ ESC │F1 Enter the concentration of the second standard e.g.
  • Page 100 The display shows: <User concentr.> Note: S2 accepted - Perform as described above to measure further S3: + ______ standards. │ ESC │ F1 │ Store - The minimum number of measured standards is - The maximum number of measured standards is 14 (S1 to S14).
  • Page 101 Enter a method number in the range from 800 to 824 e.g. [Shift] + [8] [0] [0] Confirm with [ ] key. Note: If the entered number has already been used <User polynoms.> to save a concentration, the display shows the Overwrite polynom? query: YES: 1, NO: 0...
  • Page 102 Press [F1] key to reset numerical input. Confirm with [ ] key. The display shows: <User polynoms.> A: 1.32___ E+____ Press arrow key [ ] or [ ] to change between plus and minus sign. Enter the exponent of the coefficient A e.g.
  • Page 103 Confirm with [ ] <User polynoms.> key. choose unit: >> mg/l The display shows: mmol/l mAbs Press arrow key [ ] or [ ] to µg/l change between plus and minus sign. Confirm with [ ] key. The display shows: <User polynoms.>...
  • Page 104 Press [MODE], [Shift] + [6] [6] keys. Confirm with [ ] key. The display shows: <User m. clear> choose no.:______ (800-824), (850-859) Enter the number of the use method you want to delete (in the range from 800 to 824 or 850 to 859) e.g.
  • Page 105 Confirm with [ ] key. The display shows: <User m. print> Start: Press [ ] key to print out the data (e.g. wavelength, unit …) of all stored user methods. The display shows e.g. <User m. print> M800 After data transfer the photometer goes back to mode menu automatically.

  • Page 106: Special Functions

    Press [Shift] + [0] keys to cancel without initialisation. 2.4.7 Special Functions Langelier Saturation Index (Water Balance) For calculation, the following tests are required: - pH-value - Temperature - Calcium hardness - Total alkalinity - TDS (Total Dissolved Solids) Run each test separately and note the results. Calculate the Langelier Saturation index as described: Calculation of the Langelier Saturation Index With mode 71 (see below), it is possible to select between degree Celsius and...
  • Page 107 The display shows: <Langelier> Enter the value for total alkalinity (TA) in the range Tot. alkalinity between 5 and 800 mg/l CaCO and confirm with 5 <= TA <= 800 [ ] key. + _ _ _ _ _ The display shows: <Langelier>...
  • Page 108 The entered value is too low. CH <= 1000 mg/l CaCO Confirm display message with [ ] key and enter a value in the defined range. Selection of Temperature Unit Entering the temperature value is possible in degrees Celsius or degrees Fahrenheit. Therefore the following preselection is (once) required.

  • Page 109: Instrument Basic Settings 2

    2.4.8 Instrument Basic Settings 2 Adjusting Display Contrast Press [MODE], [Shift] + [8] [0] keys. Confirm with [ ] key. The display shows: <LCD contrast> 1 ↑ 1 ↓ Store Test Press arrow key [ ] to increase the contrast of the LCD display by one unit. 0…254: 180 Press arrow key [ ] to decrease the contrast of the LCD display by one unit.
  • Page 110 The display shows: <LCD brightness> 1 ↑ 1 ↓ Store Test Press arrow key [ ] to increase the contrast of the LCD display by one unit. 0…254: 60 Press arrow key [ ] to decrease the contrast of the LCD display by one unit.

  • Page 111: Instrument Special Functions/Service

    2.4.9 Instrument Special Functions/Service Photometer Information Press [MODE], [Shift] + [9] [1] keys. Confirm with [ ] key. This method informs you about the current <System-Info> software version, about the number of performed tests and free memory capacity. Software: V2012.001.1.001.002 more: ↓, cancel: ESC Press arrow key [ ] to display the number of performed test and free memory capacity.

  • Page 112: Enclosure

    3.0 Enclosure Unpacking Carefully inspect all items to ensure that every part of the list below is present and no visible damage has occurred during shipment. If there is any damage, or something is missing, please contact your local distributor immediately. Technical Data Display...
  • Page 113 Operation Acid and solvent resistant touch-sensitive keyboard with integral beeper as acoustic indicator Power Supply 4 batteries (type AA/LR6) Lifetime: approx. 26 hours continuous use or 3500 tests Auto Off 20 minutes after last function 30 seconds acoustic signal before switch off Dimensions Approx.

  • Page 114: Abbreviations

    Abbreviations Abbreviation Definition °C Degree Celsius (centigrade) °F Degree Fahrenheit °F = (°C x 1.8) + 32 °dH Degree German Hardness °fH Degree French Hardness °eH Degree English Hardness °aH Degree American Hardness Absorption unit (≜ Extinction E) 1000 mAbs = 1 Abs ≜ 1 A ≜ 1 E µg/l (=ppb) Microgram per litre mg/l...
  • Page 115 Powder Pack Tablet Tube Test DEHA N, N-Diethylhydroxylamine Diethyl-p-phenylenediamine DTNB Ellman’s reagent 1-(2-Pyridylazo)-2-napthol PMAB Paradimethylaminobenzaldehyde PPST 3-(2-Pyridyl)-5,6-bis(4-phenylsulfonic acid)1,2,4-triazine TPTZ 2,4,6-Tri-92-Pyridyl)-1,3,5-triazine...

  • Page 116: Troubleshooting

    Troubleshooting 3.4.1 Operating Messages in the Display/Error Display Display Possible Causes Elimination Overrange Reading is exceeding If possible, the range dilute sample or use other measuring range Water sample is too Filter water cloudy sample Too much light on the Seal the cap photo cell Repeat...
  • Page 117 Battery warning Capacity of the battery is Warning signal every 3 too low; minutes change the batteries Warning signal every 12 seconds Warning signal, the Change the instrument switches batteries itself off Jus Overrange The user calibration is Please check out of the accepted the standard, range...
  • Page 118 Repeat zeroing Error absorbance Fluoride calibration Repeat e.g.: T2>T1 was not correct calibration The calculation of a Test value is not possible procedure (e.g. combined correct? chlorine) If not – repeat test Example 1: Example 1 The readings 0.6 mg/l free Cl for free and comb Cl total chlorine...
  • Page 119 not able to calculate the combined chlorine In this case, the combined chlorine is most likely the same as total chlorine Example 3: Example 3: The reading for total 0.6 mg/l free Cl chlorine is comb Cl exceeding the range. Overrange instrument is not able to...

  • Page 120: General

    3.4.2 General Finding Possible Causes Elimination Test result Chemical species not as Press arrow keys to select the deviated from required required chemical species the expected Profi-Mode is switched Switch Profi-Mode off with differentiation mode 50 e.g. for the chlorine test there is no selection between...
  • Page 121 APPLICATION NOTE T2O-AN-P60 AMMONIA (P60) METHOD The following application note explains the procedure for the detection of Ammonia (P60) using the HT1000 Photometer. Equipment:  HT1000 Photometer  10ml vial  Stirring rod  HT9 Ammonia No. 1 tablet  HT10 Ammonia No.
  • Page 122  Switch the unit on using the power key.  Select the Ammonia method by depressing the cursor keys until ‘Ammonia T ’ is displayed or use the shortcut by pressing & holding shift followed by 60 Release shift and press enter. Blank analysis: ...
  • Page 123 Sample preparation:  Add one HT9 Ammonia No.1 tablet straight from the foil to the water sample.  Crush the tablet using a clean stirring rod, until no large pieces are visible.  Add one HT10 Ammonia No.2 tablet straight from the foil to the same water sample.
  • Page 124  The Lower LOD is 0.02 mg/l (20ppb), upper LOD is 1 mg/l (1000ppb).  Tolerance: ± 0.005mg/l. Notes  For best results, rinse vials thoroughly between with deionised water. Ensure that the outside of the vials are clean, dry and free from fingerprints.
  • Page 125 APPLICATION NOTE T2O-AN-P100 CHLORINE FREE & TOTAL (P100) METHOD The following application note explains the procedure for the detection of Free and Total Chlorine (P100) using the HT1000 Photometer. Equipment:  HT1000 Photometer  10ml vial  Stirring rod  HT15 DPD No.
  • Page 126  Select the Chlorine method by depressing the cursor keys until ‘Chlorine T ’ is displayed or use the shortcut by pressing & holding shift followed by Release shift and press enter.  Depress the cursor keys until the double arrow (>>) is adjacent to the required test (diff/free/total) and press enter.
  • Page 127 Sample preparation (Free Chlorine):  Add one HT15 DPD No.1 tablet straight from the foil to the water sample.  Crush the tablet using a clean stirring rod, until no large pieces are visible.  Close the vial tightly with the cap and swirl several times until the tablet is dissolved.
  • Page 128  Crush the tablets using a clean stirring rod, until no large pieces are visible.  Close the vial tightly with the cap and swirl several times until the tablet is dissolved. Analysis (Total Chlorine):  Place the vial in the sample chamber, making sure that the marks on the instrument and vial are aligned.
  • Page 129  Place the vial in the sample chamber, making sure that the marks on the instrument and vial are aligned.  Press the Test key  Remove the vial from the sample chamber.  Add one HT16 DPD No.3 tablet straight from the foil to the water sample.
  • Page 130  For best results, rinse vials thoroughly between with deionised water. Ensure that the outside of the vials are clean, dry and free from fingerprints. Always handle vials by the lid where possible. For deep cleaning of vials, immerse in Sodium Hypochlorite solution for 1 hour and rinse with deionised water.
  • Page 131 ® APPLICATION NOTE T2O-AN-P260 NITRATE (P260) METHOD The following application note explains the procedure for the detection of Nitrate (P260) using the HT1000 Photometer. Equipment:  HT1000 Photometer  Nitrate test tube  Nitrate test powder  Nitrate Test tablet ...
  • Page 132  Consult the safety data sheet for all of the reagents before use. Even if you have used HydroTest reagents before, the formulation may have changed. Getting started:  Switch the unit on using the power key.  Select the Nitrate method by depressing the cursor keys until ‘Nitrate T ’...
  • Page 133  Ensure that the 10ml vial is clean.  Fill the vial with 10ml of the water sample.  Fit the cap and tighten.  Place the vial in the sample chamber, making sure that the arrow marks on the instrument and vial are aligned.
  • Page 134  Stand the tube upright, allow contents to settle, then gently invert three to four times.  Allow to stand for a further 2 minutes, then open and wipe carefully around the rim to remove solid particles.  Decant 10ml of the treated solution into the 10ml vial.
  • Page 135  Wait for a reaction period of 10 minutes  After the reaction period, the measurement starts and the result is displayed in mg/l Nitrate LOD/Tolerance  The Lower LOD is 0.08 mg/l (80ppb), upper LOD is 1 mg/l (1000ppb). ...
  • Page 137 ® APPLICATION NOTE T2O-AN-P270 NITRITE (P270) METHOD The following application note explains the procedure for the detection of Nitrite (P270) using the HT1000 Photometer. Equipment:  HT1000 Photometer  10ml vial  Stirring rod  Nitrite LR tablet Safety:...
  • Page 138  Consult the safety data sheet for all of the reagents before use. Even if you have used HydroTest reagents before, the formulation may have changed. Getting started:  Switch the unit on using the power key.  Select the Nitrite method by depressing the cursor keys until ‘Nitrite T ’...
  • Page 139  Ensure that the 10ml vial is clean.  Fill the vial with 10ml of the water sample.  Fit the cap and tighten.  Place the vial in the sample chamber, making sure that the arrow marks on the instrument and vial are aligned.
  • Page 140  Place the vial in the sample chamber, making sure that the marks on the instrument and vial are aligned.  Press the Test key  Wait for a reaction period of 10 minutes  After the reaction period, the measurement starts and the result is displayed in mg/l Nitrite.
  • Page 141  Interferences may occur from presence of the following ions: Antimony (III), Iron (III), Lead, Mercury (I), Silver, Chloroplatinate, Metavanadate, Bismuth.  Copper (II) ions may artificially lower test results.
  • Page 142 HydroCheck® HC1000 Multi-Parameter Electrochemical Water Tester Instruction Manual...
  • Page 143 Contents 1.1 Introduction .................... 98 1.2 Unpacking the Meter ................98 1.3 List of Accessories ................. 98 2.1 Display ....................98 2.2 Keypad ....................99 2.3 Connectors ..................100 2.4 Connecting the Sensors ............... 101 2.5 Inserting/Replacing the Batteries ............102 2.6 Refilling Electrolyte Solution for DO Probe ...........
  • Page 144 8.2 100% saturation calibration: ..............132 8.3 2 Point Calibration: ................133 9.1 DO Calibration in mg/L or ppm Mode ........... 135 9.2 2 Point Calibration: ................136 10.1 Temperature Calibration ..............137 11.1 pH Electrode Care and Maintenance ..........138 11.2 ORP Electrode Care and Maintenance ..........

  • Page 145: Introduction

    1.1 Introduction Thank you for selecting the Trace2o® HydroCheck HC1000 portable multiparameter water quality meter. This manual provides instructions to guide the user in normal operation, diagnostics and maintenance of the instrument. Please read the instructions carefully before use. 1.2 Unpacking the Meter Before unpacking, ensure that the current work environment meet following conditions.

  • Page 146: Keypad

    INDEX: Measurement mode Battery indicator Calibration Stable indicator mode Setup mode Hold indicator Memory mode Calibration Due Reminder Electrode Automatic Temperature slope indicator Compensation: 2.2 Keypad The Trace2o® HydroCheck® HC1000 has a tactile membrane keypad. Words and symbols describe functions of each key. The direction keys are multi-function left/right and up/down keys.

  • Page 147: Connectors

    INDEX: DESCRIPTION  Power the meter ON/OFF.  Holds the current measured value; press the key again to resume measuring.  In the calibration or setting mode, returns to measurement mode.  Toggles between available measurement modes (pH, mV, ORP, Ion, Conductivity, TDS, Salinity, mode Resistivity, DO).

  • Page 148: Connecting The Sensors

    2.4 Connecting the Sensors Take out the sensor from the carrying case. Ensure the connectors are clean and dry before connecting. pH Electrode  Insert the S7 connector on the pH electrode into corresponding connector on the instrument. Once pushed firmly into place, twist to ensure water tightness.

  • Page 149: Inserting/Replacing The Batteries

    DISSOLVED OXYGEN ELECTRODE  Insert the 6-pin connector on the temperature probe into the corresponding connector on the instrument, ensuring that the lugs are aligned. Once pushed firmly into place, tighten the waterproof locking collar.  Once connected, DO NOT pull on the cables. 2.5 Inserting/Replacing the Batteries The HC1000 is supplied pre-installed with the requisite battery.

  • Page 150: Refilling Electrolyte Solution For Do Probe

    2.6 Refilling Electrolyte Solution for DO Probe The Dissolved Oxygen electrode requires an electrolyte solution which must be kept topped up during normal use. To refill, follow the below procedure: 1. Unscrew the membrane cap from the bottom of the dissolved oxygen probe.

  • Page 151: Power On/Off

    3.1 Power On/Off  To turn the meter on, press the ON/OFF key.  To turn the meter off, press and hold the ON/OFF key for 3 seconds,  The meter will turn off automatically if there are no key presses within a specified time period.
  • Page 152 Select one of the above modes. Immerse the electrode in the sample solution, and stir the solution gently. Wait for the reading to stabilise, and record the value shown on the display.

  • Page 153: Conductivity/Tds/Resistivity Measurement

    3.4 Conductivity/TDS/Resistivity Measurement 1. Press the MODE key until the display shows the (Conductivity) or (TDS) or (Resistivity) indicator. 2. Rinse the conductivity electrode thoroughly with distilled water. 3. Immerse the electrode in the sample solution, and stir the solution gently. 4.

  • Page 154: Dissolved Oxygen Measurement

    3.6 Dissolved Oxygen Measurement The HC1000 is suitable for measuring dissolved oxygen in water, wastewater, brine and other liquids. If the intended application is to measure seawater or other water containing large amounts of salt, please set the salinity coefficient before use.

  • Page 155: Hold Function

    3.6 Hold Function The meter contains two data hold modes. When the Auto-Hold function is enabled, the meter will automatically sense a stable endpoint reading and hold. The ‘Hold’ indicator appears on the display. If the Auto-Hold function is disabled, press the ON/OFF key, the meter will immediately hold currently displayed value.
  • Page 156 3. Press the ▼ key, the meter shows the date and time of the reading (Format: mm-dd, hh-mm). 4. Press the ▼ key again, the display shows the stored data. 5. After viewing the memory, press the ON/OFF key to return to measurement mode.

  • Page 157: Setup Menu

    4.1 Setup Menu When in setup mode, the HC1000 displays an integrated setup menu that allows you customise to the operation of the meter to meet your measurement requirements. In the different modes, the meter will show the corresponding options. For general options, the change will be applied to all modes.
  • Page 158 ORP MODE: OPTION DEFAUL DISPLAY DESCRIPTION DESCRIPTION ● 0.1mV Set the resolution for ORP measurement. CONDUCTIVITY/TDS/SALINITY/RESISTIVITY MODES: OPTION DEFAUL DISPLAY DESCRIPTION DESCRIPTION K=0.1 Select the cell ● constant of conductivity K=10 electrode. User-Defined Select the temperature Setting Range: 2.10 0.0 to 10.0%/ coefficient of sample.
  • Page 159 measurement and calibration. Set the default Setting Range: TDS conversion 0.40 to 1.00 factor. ● Degrees Celsius Set the default Degrees temperature unit. Fahrenheit...
  • Page 160 DISSOLVED OXYGEN MODE: OPTION DEFAUL DISPLAY DESCRIPTION DESCRIPTION Select the ● 1 point number of 2 points calibration points. Select the default atmospheric Setting Range: 450 to 850mmHg pressure coefficient. Select the salinity Setting Range: coefficient of 0.0 to 50.0ppt sample solution.
  • Page 161 GENERAL OPTIONS: OPTION DEFAUL DISPLAY DESCRIPTION DESCRIPTION ● Sets the stability criteria for measurement. When the “LO” option is enabled, measuring value will stabilize quickly, but reading is less High accurate. When the “HI” option is enabled, measuring value will stabilize slowly, but improves accuracy.
  • Page 162 … 1 to 31 days When the calibration due reminder is enabled, if you not recalibrate meter within a ● Disable specified time period, the meter will automatically show indicator. Sets the date and time of the meter. Enable Clear all stored ●...

  • Page 163: Adjusting The Settings

    4.2 Adjusting the settings: 1. If necessary, press MODE key until the display shows corresponding mode indicator (e.g., pH mode). 2. Press and hold the CAL key for 3 seconds, the meter enters setup mode, the display shows adjustable setting, and parameter number. …………Setting …………Parameter number 3.

  • Page 164: Exiting Setup Mode

    5. Press ▲ or ▼ key to select the desired option. 6. Press ENTER key to confirm, the meter returns to measurement mode. Setting is completed. 4.3 Exiting setup mode: During the setup mode, if you want to exit setup menu, press MEAS key, the meter will return to measurement mode immediately.

  • Page 165: Temperature Compensation

    …………Year Enter 4. Press ▲ or ▼ key to set the year. 5. Press ENTER key to confirm, the meter shows the currently programmed date and time. The value currently being amended will be flashing. …………Format: Month - Day Enter …………Format: Hours - Minutes 6.

  • Page 166: Manual Temperature Compensation

    switched to automatic temperature compensation mode. 4.7 Manual Temperature Compensation: 4.7.1. DO NOT connect the temperature probe to meter. 4.7.2. Press and hold the MODE key for 3 seconds to enter temperature setting mode. 4.7.3. Press ▲ or ▼ key to set the temperature of the sample. 4.7.4.

  • Page 167: Single Point Calibration

    If you selected the user-defined option in the setup menu, the meter will allow only 2 point calibration. Single point calibration should only be carried out with buffers of pH 7.00, 6.86 or 6.79, otherwise calibration will not be accepted. Ensure that the meter is calibrated when attaching a new electrode or before first use.

  • Page 168: Multi-Point Calibration

    5.3 Multi-Point Calibration: 5.3.1 Make sure that you have selected between 2 and to 5 point calibration in the setup menu. 5.3.2 Repeat steps 5.2.2 to 5.2.5 above. When the first calibration point is completed, the display will show “CAL2”. The meter prompts to continue with second calibration point.

  • Page 169: Ph Calibration With User-Defined Buffers

    5.4 pH Calibration with User-Defined Buffers: Ensure that the “USER” option is selected in the setup menu. 5.4.1 N.B. Custom buffer solution values should be at least 1 pH unit apart. 5.4.2 Rinse the pH electrode with distilled water. Immerse the electrode in the custom buffer solution.

  • Page 170: Ph Calibration Report

    5.5 pH Calibration Report The meter allows checking of the calibration data of the pH electrode for diagnostic purposes. 5.5.1. Press the mR key in the pH measurement mode, the display shows “LOC/P-01”. 5.5.2. Press ▲ or ▼ key until the display shows “ELE/P-02” (Electrode Diagnosis).

  • Page 171: Orp Calibration

    6.1 ORP Calibration The HC1000 allows single point calibration in the relative mV mode, but calibration is not necessary unless and exact readout agreement with a work standard, and at a specific ORP value is needed. 1. Press the MODE key until the meter shows indicator.

  • Page 172: Orp Calibration Report

    6.2 ORP Calibration Report The meter allows checking the mV offset of the ORP electrode for diagnostic purposes. 1. Press the mR key in the ORP measurement mode, the display shows “LOC/P-01”. 2. Press ▲ or ▼ key until the meter shows “ELE/P-02” (Electrode Diagnosis). 3.

  • Page 173: Conductivity Calibration

    7.1 Conductivity Calibration The HC1000 is capable of using conductivity electrodes with different cell constants. Before use, ensure that the correct cell constant is selected (0.1, 1 or 10) in the setup menu. If the current option is “USER”, the automatic calibration function will be disabled (Refer to Setup Menu section).

  • Page 174: Single Point Calibration

    7.2 Single Point Calibration: 7.2.1 Press the MODE key until the meter shows the indicator. 7.2.2 Rinse the conductivity electrode with distilled water, then rinse with a small amount of calibration solution. 7.2.3 Press the CAL key; the meter enters the calibration mode. 7.2.4 Immerse the conductivity electrode in the calibration solution.

  • Page 175: Multi-Point Calibration

    7.3 Multi-Point Calibration: 7.3.1 Make sure that you have selected 2 to 5 points calibration in setup menu. 7.3.2 Repeat steps 1.2 to 1.5 above; when the first calibration point is completed, the display will show “CAL2”. The meter prompts you to continue with the second calibration point.

  • Page 176: Manual Calibration

    7.4 Manual Calibration: The HC1000 offers a quick manual calibration mode, allowing the user to easily calibrate the meter. 7.4.1 Record the cell constant value, which is clearly marked on a label attached to the electrode (e.g., K=1.08). 7.4.2 Ensure the Cal indicator is showing, and press and hold the CAL key for 3 seconds to enter setup menu, the display shows “CELL”...

  • Page 177: Conductivity Calibration Report

    7.4.6 Press ▲ or ▼ key to set each digit according to the value marked on the electrode. 7.4.7 Press ENTER key to confirm until the setting values stop flashing. Once calibration is complete, the meter returns to measurement mode automatically.

  • Page 178: Do Calibration In % Saturation Mode

    4. Press ▼ key, the meter shows calibration point and calibration factor (e.g., K=0.998). ……... Calibration Point ...…... Calibration Factor 5. After the browsing, press MEAS key to exit the current mode. EXIT THE CALIBRATION: To exit calibration, press the ON/OFF key at any point; the meter will return to measurement mode immediately 8.1 DO Calibration in % Saturation Mode The HC1000 dissolved oxygen probe can be calibrated quickly in air.

  • Page 179: 100% Saturation Calibration

    8.2 100% saturation calibration: 8.2.1 Press MODE key until the meter shows indicator and measurement unit “%”. 8.2.2 Make sure that 1 point calibration is selected in the setup menu. 8.2.3 Press CAL key; the meter enters calibration mode, the display shows “100%/CAL1”.

  • Page 180: Point Calibration

    8.3 2 Point Calibration: 8.3.1 Ensure that 2 point calibration is selected in the setup menu. Press CAL key, the meter shows “100/CAL1”. 8.3.2 Press ▲ or ▼ key until the display shows “0/CAL1”. 8.3.3  8.3.4 Immerse the dissolved oxygen probe into the saturated anhydrous sodium sulfite solution (zero oxygen solution).
  • Page 181 stabilise, the display shows “END”. Once calibration is complete, the meter returns to measurement mode automatically. N.B. Performing a percentage saturation calibration will simultaneously calibrate the corresponding mg/L (or ppm) concentration value. Therefore, additional mg/L calibration isn’t required in most circumstances.

  • Page 182: Do Calibration In Mg/L Or Ppm Mode

    9.1 DO Calibration in mg/L or ppm Mode 9.1.1 Press MODE key until the meter shows indicator and measurement unit “mg/L” or “ppm”. 9.1.2 Ensure that 1 point calibration is selected in the setup menu. 9.1.3 If necessary, set the salinity and atmospheric pressure coefficient in the setup menu (Refer to Setup Menu section).

  • Page 183: Point Calibration

    9.2 2 Point Calibration: 9.2.1 Make sure that you have selected 2 points calibration in the setup menu. Press CAL key, the meter shows “8.25mg/L/CAL1” (@25 9.2.2 Press ▲ or ▼ key until the display shows “0.00mg/L/CAL1”. 9.2.3  9.2.4 Immerse the dissolved oxygen probe into the saturated anhydrous sodium sulfite solution (zero oxygen solution).

  • Page 184: Temperature Calibration

    EXIT THE CALIBRATION: During the calibration process, if you want to exit calibration, press MEAS key, the meter will return to measurement mode immediately. 10.1 Temperature Calibration When automatic temperature compensation is enabled, the meter will need to be calibrated if the temperature reading displayed differs from that of an accurate thermometer.

  • Page 185: Ph Electrode Care And Maintenance

    11.1 pH Electrode Care and Maintenance The pH electrode is especially susceptible to dirt and contamination. Regular cleaning is necessary depending on the extent and condition of use. AFTER MEASURING: Rinse the pH electrode in distilled water, and store the electrode in a suitable electrode storage solution.

  • Page 186: Orp Electrode Care And Maintenance

    11.2 ORP Electrode Care and Maintenance  Ensure that the ORP electrode is thoroughly washed with distilled water after each use.  In aggressive chemicals, dirty or viscous solutions, and solutions with heavy metals or proteins, take readings quickly and rinse electrode immediately after.

  • Page 187: Conductivity Electrode Care And Maintenance

    11.3 Conductivity Electrode Care and Maintenance  Ensure that the conductivity electrode is thoroughly washed with distilled water after each use.  If there is a build-up of solids inside the measurement area of the cell, these should be removed very carefully with a cotton bud soaked in solvent, taking care not to touch the metal parts of the inner cell.

  • Page 188: Troubleshooting

    12.1Troubleshooting CAUSE CORRECTIVE ACTION DISPLAY Soak the pH electrode in 3M KCL solution for 15 minutes Electrode dried out Soak the conductivity electrode in tap water for a few minutes Measured value is out of Check the electrode whether range clogged, dirty or broken Incorrect calibration Using the fresh calibration...

  • Page 189: Appendix 1: How To Calculate The Temperature Coefficient

    13.1 Appendix 1: How to calculate the temperature coefficient To determine the temperature coefficient of sample solution, use the following formula: ×100% -25)-C -25) Where: =Temperature coefficient =Conductivity at Temperature A =Conductivity at Temperature B =Temperature A =Temperature B 1. Press and hold the MODE key for 3 seconds to enter temperature setting mode.

  • Page 190: Appendix 2: How To Calculate The Tds Conversion Factor

    7. Calculate the temperature coefficient of sample solution according to the formula shown above. 8. Enter the calculated temperature coefficient into the meter. 13.2 Appendix 2: How to calculate the TDS conversion factor To determine the TDS conversion factor, use the following formula: Actual TDS Factor= Actual Conductivity @ 25ºC...

  • Page 191: Appendix 3: Pressure Vs Altitude Table

    13.3 Appendix 3: Pressure vs Altitude Table ALTITUDE (m) mmHg 101.3 100.1 98.8 97.6 96.4 95.2 94.0 92.8 91.7 90.5 1000 89.4 1100 88.3 1200 87.2 1300 86.1 1400 85.0 1500 84.0 1600 82.9 1700 81.9 1800 80.9 1900 79.9 2000 78.9...

  • Page 192: Warranty

    14.1 Warranty Trace2o hopes that the HydroCheck HC1000 will give many years of trouble- free operation, but in the event of a technical problem occurring the instrument is covered by the Trace2o Ltd standard warranty terms and conditions available via email or via download from www.trace2o.com. In the event that any technical assistance is required Trace2o Customer service department will be happy to assist.
  • Page 193 NOTES...

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