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Summary of Contents for Dionex UVD 170S
- Page 1 UV/VIS Detector UVD 170S/340S Operating Instructions Version: 3.01 Date: July 14, 2000 © Dionex Softron 2000 Doc.: UVD170SUVD340_OI_E_V301.doc...
- Page 2 Declaration of Conformity Product: UV/VIS Detector Type: UVD 170S/340S Dionex Softron GmbH herewith declares conformity of the above products with the respective requirements of the following regulations: • EN 50081-1 : 1992: Electromagnetic Compatibility (EMC) - Generic emissions standard Part 1: Residential, commercial and light industry •...
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Page 3: Table Of Contents
Flow Cell Versions ....................18 6.3.2 Flow Cell Removal....................21 6.3.3 Flow Cell Replacement ..................21 6.3.4 Flow Cell Cleaning....................21 Technical Specifications ....................23 Accessories and Spare Parts ................... 24 Standard Accessories, UVD 170S/340S ..............24 Spare Parts........................24... -
Page 4: Introduction
The layout of this manual is designed to provide quick reference to the appropriate sections, according to the operation required. However, it is recommended that, before operating the UVD 170S/340S, the manual should be read thoroughly and completely in order to obtain a full understanding of the instrument. -
Page 5: Intended Use Of This Instrument
The UVD 170S/340S is a high-sensitivity UV-VIS diode array detector and is specifically intended for use only for HPLC analysis and only under the control of the CHROMELEON Data System. Dionex shall not be liable for any damages, material or otherwise, which are caused by inappropriate or improper use of this device. -
Page 6: Installation
Operating Instructions UVD 170S/340S 2 Installation 2.1 Positioning and Environment The location of the instrument should conform to the following: • stable and free of vibration • free of large temperature variation and draughts • away from direct sunlight •... -
Page 7: Fuse Replacement
Operating Instructions UVD 170S/340S 2.2.1 Fuse Replacement • Switch off the unit and disconnect the power cable, • draw out the fuse cartridge using a small screwdriver (→ fig. 1), • replace fuses as appropriate. Important: Only use fuses as indicated in fig. 1 (min. voltage 250 V) or those listed in the original parts list. -
Page 8: Fluidic Connections
Operating Instructions UVD 170S/340S 2.3 Fluidic Connections The capillary connections of the UVD 170S/340S are located on the left side of the detector (→ fig. 6). The input capillary is marked by the black color. Connect this capillary to the column output. -
Page 9: Installation On The Pc-Interface Board
An open switch (OFF) indicates a binary "1", while a closed switch (ON) indicates a binary "0". On the right, the values are converted into hexadecimal values. Determine the address by adding the values. The default setting of the UVD 170S/340S interface board therefore has the following base address:... -
Page 10: Overview
Operating Instructions UVD 170S/340S 3 Overview The UVD 170S/340S is a high-performance diode-array detector for HPLC detection in the UV/VIS wavelength range. The unit has been developed exclusively for operation under the CHROMELEON chromatography data system. The UVD 170S/340S features: •... -
Page 11: Operation
Stand-alone operation is not possible. Data transfer is performed digitally, via a special interface cable (→ section 2.4). 4.1 Switching the Unit On/Off Having ensured all cable connections to the UVD 170S/340S, the unit can be switched on via the power switch on the rear of the unit. Please note: The deuterium lamp is not automatically switched on. -
Page 12: Installation And Operation Within Chromeleon
Operating Instructions UVD 170S/340S 4.3 Installation and Operation within CHROMELEON 4.3.1 Communication Port The UVD 170S/340S is controlled via a special PC-interface board (→ section 2.4). 4.3.2 Required CHROMELEON Modules To be able to control the detector UVD 170S/340S via CHROMELEON... - Page 13 Operating Instructions UVD 170S/340S Tab Dialog Box "Signals" Option Description UV_VIS_1 UVD 170S / UVD 340S UV_VIS_2 UVD 170S / UVD 340S UV_VIS_3 UVD 170S: from CHROMELEON v.4.10 / UVD 340S UV_VIS_4 UVD 170S: from CHROMELEON v.4.10 / UVD 340S...
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Page 14: Operation Within Chromeleon Tm
Operating Instructions UVD 170S/340S 4.3.4 Operation within CHROMELEON In CHROMELEON , there are two principal modes of instrument operation: • direct control via the toolbar and menu bar or via controls in the Control Panel • control via time programs (PGM-file or command buttons) The following signal-specific and global commands are available. -
Page 15: Spectra Calibration Under Chromeleon Tm
Operating Instructions UVD 170S/340S 4.3.5 Spectra Calibration under CHROMELEON Under CHROMELEON , spectra calibration is performed automatically after each "Lamp on" or "Connect" command ("detector calibration"). The command "CheckWavelength" allows you to include the largest wavelength deviation of this calibration in the Audit Trail. A calibration is possible only when certain conditions are met: •... -
Page 16: Troubleshooting
(see below). Connecting cable between Connectors on the detector UVD 170S/340S and PC not and the PC must be inserted correctly installed evenly and locked into place with latching devices on both sides. - Page 17 Wrong wavelength Select suitable wavelength Connecting cable between Connectors on the detector UVD 170S/340S and PC not and the PC must be inserted correctly installed evenly and locked into place with latching devices on both sides.
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Page 18: Maintenance
Any other service or maintenance work should be carried out only by a qualified Dionex personnel. In the event that the UVD 170S/340S should require shipment for the purpose of service, this should be only in the original packing. If no original packing is available, this can be acquired from Dionex or your local Dionex representative. - Page 19 Operating Instructions UVD 170S/340S Fig. 6: Side view, UVD 170S/340S (side panel open)
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Page 20: Lamp
Operating Instructions UVD 170S/340S 6.2 Lamp Important: Use only the original Dionex deuterium lamp. Using third party parts may damage the instrument; and the warranty coverage shall become invalid. 6.2.1 Lamp Removal • If the UVD 170S/340S is still connected to CHROMELEON... -
Page 21: Flow Cell
Standard Flow Cell (Order no. 5065.1810) The standard flow cell of the UVD 170S/340S has a volume of 10µl and a path length of 9mm. The flow cell body is composed of PEEK with quartz windows and is thus 100% biocompatible. - Page 22 Operating Instructions UVD 170S/340S Fig. 7: Standard Flow Cell Description Order No. Screw M3x12 DIN965 included in 5065.1815 Heat exchanger included in 5065.1815 PEEK tubing ID=0.5mm 2251.6002 PEEK fitting screw 1/16", 15 mm included in 5065.1815 Screw M3x23 DIN 965 included in 6065.1800...
- Page 23 Preparative Flow Cell (Order no. 5065.1800P) The preparative flow cell for the UVD 170S/340S has a volume of 6µl and a path length of 2mm. The cell body is composed of PEEK and is thus 100% biocompatible. The maximum pressure rating of the flow cell is 100bar (10,000kPa).
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Page 24: Flow Cell Removal
Operating Instructions UVD 170S/340S 6.3.2 Flow Cell Removal • If the UVD 170S/340S is still connected to the data system, first terminate communication using the Disconnect command in CHROMELEON (→ CHROMELEON manual). • Switch off the detector. Disconnect the power cord. - Page 25 Operating Instructions UVD 170S/340S Important: Never attempt to remove dirt from the flow cell body using a spatula, tweezers or any other objects as this may lead to irreparable damage. • Replace the lenses onto either side of the flow cell body so that the flat faces are on the inside.
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Page 26: Technical Specifications
Operating Instructions UVD 170S/340S 7 Technical Specifications Optics: Single beam principle with simultaneous measurement across the photodiode array. Light source: Deuterium lamp Wavelength range: 200nm - 344nm (UV-range) 345nm - 595nm (VIS-range) Pixel bandwidth: 1.9nm (UV-range) 3.3nm (VIS-range) Wavelength accuracy: ±... -
Page 27: Accessories And Spare Parts
Spare parts and accessories are always maintained at the latest technical standard. Therefore, order numbers are subject to alteration. However, updated parts will always be compatible with the parts they replace. 8.1 Standard Accessories UVD 170S/340S The following standard accessories (order no. 5065.9000) are included in the shipment: Order No.*... - Page 28 Operating Instructions UVD 170S/340S Order No.* Description 5065.1815 Heat exchanger, flow cell, analyt., confg. including: Heat exchanger PEEK fitting screw 1/16", 15 mm Capillary tube 1,58x0,25 ID Single-part hand-tight fitting Screw M3x12 DIN965 (2 pcs). 5065.1820 Standard flow cell UVD170/340, 10 µl, PEEK capillaries, complete, 6065.1800...
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