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Dionex PDA-100 Operator's Manual

Photodiode array detector
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PDA-100 PHOTODIODE ARRAY DETECTOR
OPERATOR'S MANUAL
©2003 Dionex Corporation
Document No. 031644
Revision 02
September 2003

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  • Page 1 PDA-100 PHOTODIODE ARRAY DETECTOR OPERATOR'S MANUAL ©2003 Dionex Corporation Document No. 031644 Revision 02 September 2003...
  • Page 2 Dionex Corporation, 1228 Titan Way, Sunnyvale, California 94088-3603 U.S.A.

  • Page 3: Table Of Contents

    Contents 1 • Introduction Product Overview .........1-1 Theory of Photodiode Array Detection .
  • Page 4 PDA-100 Photodiode Array Detector Routine Operation ......... 3-5 3.3.1...
  • Page 5 Contents 4.15.2 Software Diagnostics and Calibration ....4-14 5 • Service Liquid Leaks ..........5-1 Cleaning the Flow Cell .
  • Page 6 PDA-100 Photodiode Array Detector Unpacking ..........B-1 Installation .

  • Page 7: Introduction

    UV range (190 nm to 380 nm) and a tungsten lamp optimizes the visible range (380 nm to 800 nm). When using the PDA-100, up to five single wavelengths (2D chromatograms) can be collected without being required to collect 3D data. Collecting individual...

  • Page 8: Theory Of Photodiode Array Detection

    PDA-100 Photodiode Array Detector To take full advantage of the capabilities of the PDA-100, order the 3D option for the software. The 3D option is required in order to perform the following: • 3D data presentation and spectral analysis (real-time or post-run) •...
  • Page 9 A PDA-100, under the control of Dionex software with the 3D option, correctly identified and differentiated the components. The spectra of pyrene and the impurity (see Figure 1-2) clearly indicate that the two peaks are different components.
  • Page 10 PDA-100 Photodiode Array Detector Pyrene Impurity Figure 1-1. Overlay of Pyrene and an Impurity Impurity Pyrene Figure 1-2. Spectra of Pyrene and an Impurity Doc. 031644-02 9/03...

  • Page 11: About This Manual

    1 • Introduction About This Manual Chapter 1 Provides a brief overview of the PDA-100 and the theory Introduction of photodiode array detection. Summarizes the organization of the user’s manual. Explains the meaning of safety messages and icons in the manual and the safety labels on the detector.

  • Page 12: Safety Messages And Notes

    1.3.1 Safety Messages and Notes This manual contains warnings and precautionary statements that can prevent personal injury and/or damage to the PDA-100 when properly followed. Safety messages appear in bold type and are accompanied by icons, as shown below. Indicates an imminently hazardous situation which, if not avoided, will result in death or serious injury.
  • Page 13 1 • Introduction Messages d’avertissement en français Signale une situation de danger immédiat qui, si elle n'est pas évitée, entraînera des blessures graves à mortelles. Signale une situation de danger potentiel qui, si elle n'est pas évitée, pourrait entraîner des blessures graves à mortelles. Signale une situation de danger potentiel qui, si elle n'est pas évitée, pourrait entraîner des blessures mineures à...

  • Page 14: Safety Labels

    PDA-100 Photodiode Array Detector Safety Labels The TUV GS, C, US Mark safety label and the CE Mark label on the PDA-100 indicate that the PDA-100 is in compliance with the following standards: EN 61010-1:1993 (safety), CAN/CSA-C22.2 No. 1010.1-92 (safety), UL 3101- 1/10.93 (safety), EN 50082-1:1992 (susceptibility), and EN 55011:1991...

  • Page 15: Description

    2 • Description Front Panel Figure 2-1 illustrates the front panel of the PDA-100 Photodiode Array Detector. switch provides on/off control of the detector power. LED indicators Power identify the status of four functions: the detector power, the deuterium and tungsten lamps, and the run.
  • Page 16 PDA-100 Photodiode Array Detector LED Status Function Tungsten Lighted The tungsten lamp is on. Flashing A command was issued to turn on the tungsten lamp, but the lamp failed to turn on (see Section 4.5). The tungsten lamp is off.

  • Page 17: Inside Front Panel

    NOTE For an illustration of the inside front panel with the flow cell cover and lamp cover removed, see Figure 5-1. Grasp the PDA-100 front panel by the sides and pull straight off to access the inside front panel (see Figure 2-2).

  • Page 18: Optical System

    PDA-100 Photodiode Array Detector Optical System Figure 2-3 is a schematic of the PDA-100 optical system. Light from the tungsten lamp is focused through an opening in the internal structure of the deuterium lamp. Light from the tungsten and deuterium lamps is then focused through the flow cell by the source lens.
  • Page 19 2 • Description Optical System Function Component Tungsten Lamp The light source for visible and near-infrared wavelengths (380 nm to 800 nm). The tungsten lamp is focused through an opening in the internal structure of the deuterium lamp; this allows the lamps to share the optical axis to the source lens. Visible Focus Lens Focuses the visible light from the tungsten lamp to the through- hole of the deuterium lamp.

  • Page 20: Flow Cell

    2.3.1 Flow Cell The PDA-100 flow cell assembly (see Figure 2-4) is available in PEEK or stainless steel. Two built-in heat exchangers, one on the cell inlet tubing and one on the cell handle, help stabilize the temperature of the mobile phase before it enters the cell.

  • Page 21: Electronics

    Power Supply The power supply module contains connections to the AC mains and provides regulated, low-voltage power for the PDA-100. The power supply is rated at 130 W. Power entry components are shielded and filtered to prevent electromagnetic interference (EMI).
  • Page 22 PDA-100 Photodiode Array Detector Lamp Power Supply The lamp power supply controls the deuterium and tungsten lamps; it also supplies power to the variable speed fan that cools the optical bench. NOTE The fan speed is controlled automatically. Changes in...
  • Page 23 2 • Description Data Processing Card The PDA-100 Moduleware resides on the Data Processing card. Four BNC connectors on the card provide the analog (recorder) outputs. Select the output scale, polarity, baseline offset, and filter rise time from PeakNet 6 or Chromeleon.

  • Page 24: Rear Panel

    CHANNEL 3 CHANNEL 4 TECTION AGAINST Apparatus and products manufactured and sold by Dionex Corporation under one or more of the following U.S. Patents owned or licensed by Dionex: 4,242,097; 4,252,644; 4,314,823; RISK OF FIRE OR 4,351,909; 4,383,047; 4,455,233; 4,474,664; 4,482,967; 4,512,245; 4,644,807; 4,700,059; 4,751,004;...
  • Page 25 Stellen Sie sicher, daß sich die Steckdose nahe am Gerät befindet und leicht zugänglich ist. Fuses The PDA-100 uses two 4.0 amp fast-blow fuses (IEC 127 type 1, P/N 954763). See Section 5.4 for fuse replacement instructions. For continued protection against risk of fire or shock, replacement fuses must be the type and rating specified here.
  • Page 26 The analog outputs continue to output data even after the host computer is turned off or is disconnected from the PDA-100. When the detector power is turned on again, the analog output settings are either the factory defaults (see Section 3.5) or the settings selected the last time the detector was controlled by the host computer.

  • Page 27: Operation And Maintenance

    3.1.1 Mobile Phases Solvent quality significantly affects detection limits and instrument performance. To ensure optimal performance of the PDA-100, follow the precautions below. Strong bases can etch the silica windows of the flow cell. If the mobile phase is a base, make sure the mobile phase concentration does not exceed 0.1 M.

  • Page 28: Solvent Delivery System

    PDA-100 Photodiode Array Detector • When changing from a buffer to a different operating mobile phase, be sure the solvents are miscible and will not induce precipitation of the buffers. Flush the cell with deionized water immediately after the analysis; do not let buffers remain in the cell for extended periods.
  • Page 29 3 • Operation and Maintenance • Cleanliness is very important. After operating in detection modes that do not require optically clean reagents (such as conductivity), the solvent delivery system may need to be thoroughly cleaned. A basic cleaning procedure is described below; for additional information, refer to the pump user’s manual.

  • Page 30: Getting Started

    PDA-100 Photodiode Array Detector Getting Started Before beginning operation of the PDA-100, check the following: • Press the switch on the front panel to turn on the detector (see Power Figure 2-1). • Make sure the LED is lighted, but is not flashing. If the...

  • Page 31: Routine Operation

    3 • Operation and Maintenance Routine Operation During routine operation, the PDA-100 is controlled from a host computer running either PeakNet 6 or Chromeleon software. The following sections describe the two modes of software control: panel control (in which commands are executed directly from a control panel) and automated control (control from a PGM file).
  • Page 32 9. Data collected during the run may be saved to a sequence. To retrieve the data, select the assigned sample name within the sequence. Acquisition On/Off Serial Numbers Setup Parameters Detector Signals for UV1 and UV2 Wavelengths Pump Pressure Chromatogram(s) Limits Figure 3-1. PDA-100 2D Control Panel Example Doc. 031644-02 9/03...
  • Page 33 3 • Operation and Maintenance Figure 3-2. PDA-100 3D Control Panel Example Doc. 031644-02 9/03...
  • Page 34 The table below lists several of the most frequently used detector commands. For more information, refer to the online Help. PDA-100 Command Function Connect Indicates whether the PDA-100 is connected to the server and the selected timebase. UV Lamp On/Off Turns the deuterium lamp on and off. Vis Lamp On/Off Turns the tungsten lamp on and off.

  • Page 35: Programmed Control

    Programmed Control A PGM file is a list of time-precise control commands created by the user for automated operation of the PDA-100. The user can create PGM files automatically (with the help of the PGM Wizard) or manually (by editing an existing PGM file).
  • Page 36 PDA-100 Photodiode Array Detector Creating a New PGM File 1. PeakNet 6 and Chromeleon contain a Program Wizard to facilitate the creation of new PGM files. To activate the Wizard, go to the File menu and select the New command.
  • Page 37 If the selected timebase includes other devices (pump, autosampler, etc.), Program Wizard screens for these devices are displayed before the PDA-100 screens. If necessary, click the Next button in each screen for another device until the following screen is displayed.
  • Page 38 PDA-100 Photodiode Array Detector The following screen is displayed. 9. Select the Data Collection Rate and the Rise Time. NOTE The PDA-100 performance can be optimized by careful selection of the data collection rate, rise time, and other operating parameters. For details, refer to Section 3.4.
  • Page 39 3 • Operation and Maintenance The following screen is displayed. 12. Select the sample Wavelength, Reference Wavelength, and Reference Bandwidth. NOTE The Reference Wavelength plus one-half the Reference Bandwidth cannot be outside the 3D Wavelength range. If the values are outside this range, the Reference Wavelength setting will default to the longest wavelength possible given the bandwidth.
  • Page 40 PDA-100 Photodiode Array Detector The following screen is displayed. 14. Program the single-wavelength channels by selecting the Wavelength, Bandwidth, Reference Wavelength, and Reference Bandwidth for each channel. NOTE The settings selected for UV_VIS_1 through UV_VIS_4 apply to both analog outputs and digital outputs.

  • Page 41: Optimizing Detector Performance

    3 • Operation and Maintenance Optimizing Detector Performance The performance of the PDA-100 can be optimized by careful selection of the operating parameters. Section 3.4 defines several operating parameters, describes how they interact with each other, offers guidelines for selecting them, and includes example chromatograms.
  • Page 42 PDA-100 Photodiode Array Detector Rise Time Rise time is a measure of how quickly the PDA-100 responds to a change in signal. The rise time can be set to 0.1, 0.2, 0.5, 1.0, 2.0 (default), or 5.0 seconds. The selected rise time is applied to all data collected, and to both analog and digital output.
  • Page 43 Data Collection Rate The data collection rate (or sample rate) is the number of data points per second (Hz) at which the host computer stores data from the PDA-100. (The detector electronics always collect data at 10.0 Hz.) The data collection rate can be set to 0.25, 0.5, 1.0, 2.0, 2.5 (default), 5.0, or 10.0 Hz.
  • Page 44 PDA-100 Photodiode Array Detector Sample Wavelength The PDA-100 measures absorbance over all wavelengths from 190 nm to 800 nm. The deuterium lamp optimizes the UV range (190 nm to 380 nm). The tungsten lamp optimizes the visible range (380 nm to 800 nm). Set the sample wavelength at the wavelength with the absorbance maxima.
  • Page 45 3 • Operation and Maintenance Sample Bandwidth The sample bandwidth is the wavelength range at which the chromatogram is measured. As the bandwidth increases, baseline noise decreases. Increasing the bandwidth increases the number of wavelength signals overlaid at each point in time.
  • Page 46 There are two types of reference mode: “active” and “off.” • To select the active reference mode, enter a wavelength within the scanning ability of the PDA-100. The selected wavelength will be used as the reference wavelength. The reference intensity will vary over time, as signal intensity changes.
  • Page 47 3 • Operation and Maintenance Example Chromatogram: Selecting the Reference Wavelength Figure 3-5 shows results obtained from two different channels within the same run. When the reference wavelength is turned off, baseline drift is - µ AU/hour; when a reference wavelength (active mode) is selected, µ...
  • Page 48 PDA-100 Photodiode Array Detector Reference Bandwidth The reference bandwidth, which is analogous to the conventional bandwidth of a channel, serves to average several diode signals of the reference wavelength. A reference bandwidth is required only when a reference wavelength (active mode) is selected.
  • Page 49 3 • Operation and Maintenance Example Chromatogram: Selecting the Sample Wavelength, Reference Wavelength, and Reference Bandwidth Figure 3-6 shows the spectrum of caffeine. The operating conditions are: Sample wavelength = 272 nm Sample bandwidth = 5 nm Reference wavelength = 325 nm Reference bandwidth = 51 nm These settings calculate the absorbance by the following equation: avg I...
  • Page 50 Step The appropriate PDA-100 step setting is automatically selected by the software. A step is the time interval between two successively stored data points. The smaller the step, the more data points that are recorded and, in general, the more precise the analytical results.
  • Page 51 3 • Operation and Maintenance Step Override Selecting an inappropriate step setting may cause loss of sensitivity, skipped data points, and other problems. Guidelines for overriding the automatic selected step setting are provided for reference by advanced users only. In almost all cases, the step setting selected by PeakNet 6 or Chromeleon is preferred.
  • Page 52 PDA-100 Photodiode Array Detector Negative Absorbance NOTE The NegativeAbsorptionLevel command is available only if you are running PeakNet 6.40 SP3 (or later) or Chromeleon 6.50 SP1 (or later) and PDA-100 Moduleware version 2.0 (or later). Negative absorbance is the result of decreased absorption of the eluent after autozero (which usually occurs at the beginning of a run).
  • Page 53 3 • Operation and Maintenance In PeakNet 6 or Chromeleon, press F8 to display the Commands dialog box. Select UV, select the NegativeAbsorptionLevel command, and select a higher setting. You may continue increasing the setting if the range is required. Baseline noise may increase as the NegativeAbsorptionLevel setting increases.

  • Page 54: Stand-Alone Analog Operation

    Data is presented at the analog output channels whenever the detector power is on. Use a chart recorder or other voltage-measuring device to monitor the channels. When the PDA-100 power is first turned on, the analog output settings are at the factory defaults (see the table below). Channel...

  • Page 55: Routine Maintenance

    3 • Operation and Maintenance Routine Maintenance The following maintenance procedures may be performed by the user. Other maintenance procedures must be performed by trained Dionex Service personnel. • Before shutting down the detector for more than 24 hours, flush the system with deionized water or methanol for at least 15 minutes at 1.0 mL/min (or...
  • Page 56 PDA-100 Photodiode Array Detector • Replace the deuterium and tungsten lamps when required (see below). The average lamp lifetime is 1000 hours. However, lamps often last longer than this with only minimal deterioration in performance. Monitor baseline noise. If the noise increases above an acceptable level, especially after a lamp(s) has been in service for more than 1000 hours, replace the lamp.

  • Page 57: Troubleshooting

    If you are unable to eliminate a problem, contact Dionex. In the U.S., call 1-800- 346-6390 and select the Technical Support option. Outside the U.S., call the nearest Dionex office.

  • Page 58: No Detector Response

    PDA-100 not detected on the DX-LAN When the host computer is powered up, the software scans the DX-LAN for all installed instruments. If the PDA-100 power is off, the detector cannot be identified. Before turning on the computer, make sure the detector power is already turned on.

  • Page 59: Noisy Baseline

    DX-LAN interface card. When using 10BASE-T cabling, always install an Ethernet hub. If you simply plug the 10BASE-T cable from the PDA-100 into the connector on the host computer’s DX-LAN card, the connection will not work.
  • Page 60 Inappropriate reference wavelength Select a reference wavelength in a quiet area of the spectrum where little absorption occurs. Dionex recommends selecting a reference wavelength below 380 nm for UV applications and above 600 nm for visible applications. For more information, see Section 3.4.
  • Page 61 Flow cell cover, lamp cover, or front panel is missing Optical components are sensitive to temperature and light. Do not operate the PDA-100 unless the flow cell cover, lamp cover, and front panel are in place. • Crimped or plugged tubing Over time, tubing may become pinched off and plugged.

  • Page 62: Drifting Baseline

    After installing a new lamp, allow from 8 to 24 hours for the lamp to stabilize. • Lamp cover, flow cell cover, or front panel is missing Do not operate the PDA-100 unless the lamp cover, flow cell cover, and front panel are in place; optical components are sensitive to temperature and light. •...

  • Page 63: Lamp Does Not Light Or Lamp Led Is Flashing

    Select a reference bandwidth in an area of the spectrum where little absorption occurs (see Section 3.4). Dionex recommends selecting a reference wavelength (active reference mode) for gradient analyses and turning off the reference wavelength for isocratic analyses in a laboratory with a stable ambient temperature. For more information, see Section 3.4.

  • Page 64: Wavelength Calibration Fails

    Wellness panel (see Section 4.15.2). If these fail, remove the flow cell and repeat the wavelength calibration and the wavelength verification. If the wavelength calibration still fails, contact Dionex Technical Support for assistance. Doc. 031644-02 9/03...

  • Page 65: Wavelength Verification Fails

    4 • Troubleshooting Wavelength Verification Fails • Detector wavelength requires recalibration In PeakNet 6 or Chromeleon, select the UV Calibration command on the Wellness panel. Or, press F8 to display the Commands dialog box and select UV (to display UV-related commands). Select the UV_Calibration command, and then select WavelengthCal.

  • Page 66: Faulty Dx-Lan Communication

    Select a spectral range in the PGM file or control panel. • Detector and PGM file (or control panel) connected to different timebases Add the PDA-100 to the same timebase as the PGM file or control panel. 4.11 Low Spectral Resolution • Bunch width too wide Select a bunch width setting of 1 nm and then recheck the resolution.

  • Page 67: Peaks Too Large Or Small

    4.12 Peaks Too Large or Small • Analog output-related problem Check the recorder and integrator input voltage. The PDA-100 provides a 1 volt full-scale output. Verify that the selected output range (AU full-scale) is appropriate. •...

  • Page 68: Poor Peak Shape

    PDA-100 Photodiode Array Detector 4.13 Poor Peak Shape • Concentration of standard is too high; column is overloaded Dilute the standard. Install a sample loop with a smaller volume. • Concentration of standard is too low Increase the standard concentration.

  • Page 69: Error Message: Not Enough Light To Perform Operation

    (see Section 5.2). Reinstall the cell in the optical bench and autozero the detector again to verify that the problem has been resolved. If the error message appears again, contact Dionex Technical Support for assistance. If you see a weak light (or no light at all) shining onto the business card, the lamp intensity is too low.

  • Page 70: Diagnostics

    Audit Trail includes the date, time, and error code for each problem the PDA-100 reports. There are two ways to review this information: • Check the Audit Trail displayed in the PDA-100 control panel, or • Retrieve the Audit Trail from a sequence by selecting the Audit Trail folder and the file for that date.
  • Page 71 Select UV (to display UV-related commands). c. Select UV_Calibration and then WavelengthCal. d. Click the Execute button. e. When the calibration procedure is complete, select the WavelengthCalResult command, or click the Wavelength button on the PDA-100 Wellness control panel. 4-15 Doc. 031644-02 9/03...
  • Page 72 PDA-100 Photodiode Array Detector 4-16 Doc. 031644-02 9/03...

  • Page 73: Service

    This chapter describes routine service procedures that the user may perform. All procedures not described here must be performed by trained Dionex Service personnel. To contact Dionex in the U.S., call 1-800-346-6390 and select the Technical Support option. Outside the U.S., call the nearest Dionex office.

  • Page 74: Cleaning The Flow Cell

    PDA-100 Photodiode Array Detector Cleaning the Flow Cell Film deposits on the flow cell windows may cause excessive baseline noise or high absorbance offset. 1. Pump methanol through the cell for 20 to 30 minutes at 1.0 mL/min. 2. Pump deionized water through the cell for 20 to 30 minutes at 1.0 mL/min.

  • Page 75: Removing The Flow Cell

    5 • Service Removing the Flow Cell Remove the flow cell from the optical bench prior to inspecting the cell for leaks or obstructions, replacing the cell windows (see Section 5.5), or installing a new cell (see Section 5.6). 1. Grasp the detector front panel by the sides and pull straight off to remove. 2.

  • Page 76: Replacing The Flow Cell Windows

    PDA-100 Photodiode Array Detector Replacing the Flow Cell Windows Contaminated flow cell windows can significantly increase baseline noise and cause wavelength calibration to fail. If cleaning the cell (see Section 5.4) does not eliminate these problems, replace the two silica flow cell windows.

  • Page 77: Replacing The Flow Cell

    5 • Service 5. Use tweezers to carefully remove the O-ring from the groove (see Figure 5-2). Do not scratch the groove. Scratches in the groove will prevent the new O-ring from sealing properly and will cause leaks. 6. Install a new O-ring (P/N 054136) in the groove. 7.

  • Page 78: Replacing The Deuterium Lamp

    Replacing the Deuterium Lamp 1. Stop the PeakNet 6 or Chromeleon server. 2. Turn off the PDA-100 power switch. The lamp housing and base may be hot to the touch, especially after the lamp has been in operation for a long time. Wait until the lamp has cooled down before continuing.
  • Page 79 5 • Service 12. When the lamp is fully seated, tighten the screws in the flange with a screwdriver. To ensure proper performance, the lamp must be fully seated. 13. Squeeze the clip on the UV lamp connector and insert it into the 6-pin bulkhead connector on the side wall.
  • Page 80 PDA-100 Photodiode Array Detector 20. Reset the lamp lifetime as follows: a. Press F8 to display the Commands dialog box. b. Select UV (to display UV-related commands). c. Select UVLampAge. d. Reset the lamp age to zero. e. Click the Execute button.

  • Page 81: Replacing The Tungsten Lamp

    Replacing the Tungsten Lamp 1. Stop the PeakNet 6 or Chromeleon server. 2. Turn off the PDA-100 power switch. The lamp housing and base may be hot to the touch, especially after the lamp has been in operation for a long time. Wait until the lamp has cooled down before continuing.
  • Page 82 PDA-100 Photodiode Array Detector 11. Carefully insert the new lamp into the optical bench and tighten the three screws that secure the lamp to the optical bench. (The replacement lamp is pre-aligned in the mount.) To ensure proper performance, the lamp must be fully seated.

  • Page 83: Replacing The Main Power Fuses

    Netzbuchse auf der Rückseite des PDA-100. 1. Turn off the PDA-100 power switch. 2. Disconnect the power cord from the PDA-100 rear panel. 3. The fuse holder is part of the main power receptacle (see Figure 5-4). Note the recessed lock on each side of the fuse holder;...
  • Page 84 4. When both locks are released, pull the fuse holder straight out of the compartment and replace the two fuses in the holder with new 4.0 amp fast- blow IEC 127 fuses (P/N 954763). Dionex recommends always replacing both fuses.

  • Page 85: A • Specifications

    A • Specifications A.1 Physical Dimensions 17.5 cm H x 38.6 cm W x 49.0 cm D (6.9 x 15.2 x 19.3 in) 6 cm (2.4 in) clearance required behind the detector Weight 16 kg (35 lbs) Decibel Level <50 db (at “A WEIGHTING” setting) during routine operation 56 db (at “A WEIGHTING”...

  • Page 86: Detector

    PDA-100 Photodiode Array Detector A.4 Detector Optical System Single-beam, reverse-optics design with concave holographic grating Light Sources Deuterium lamp (30 W) for ultraviolet spectrum analysis Tungsten lamp (15 W) for visible spectrum analysis Photodiode Array 1024 elements Wavelength 190 nm to 800 nm...

  • Page 87: Flow Cells

    A • Specifications A.5 Flow Cells Cell Body PEEK or stainless steel PEEK cell: 13 µL volume; 10 mm (0.394 in) path length Volume and Stainless steel cell: 13 µL volume; 10 mm (0.394 in) path length Optical Path Length Maximum PEEK cell: 2 MPa (300 psi) Operating...
  • Page 88 PDA-100 Photodiode Array Detector Doc. 031644-02 9/03...

  • Page 89: B • Installation

    • Do not turn on the PDA-100 unless it is at room temperature (10 ºC to 40 ºC; 50 ºF to 104 ºF). If the detector is moved from a cold environment to a warm environment, wait at least 1 hour for condensation to evaporate before turning on the mains power.

  • Page 90: Installation

    (IEC 320 C13) from the PDA- 100 main power receptacle (see Figure B-1) to a grounded, single-phase power source. Use the PDA-100 power switch (see Figure 2-1) to turn the detector on and off.
  • Page 91 The flow cell is installed in the detector at the factory. Follow the steps below to complete the cell installation. 1. Grasp the PDA-100 front panel by the sides and pull straight off to remove. 2. Connect the cell inlet tubing to the union fitting on the heat exchanger tubing (see Figure B-2).

  • Page 92: Flow Cell

    (0.14 MPa to 0.34 MPa; 20 psi to 50 psi) required to help prevent bubbles from forming in the cell. a. Locate the following items in the PDA-100 Ship Kit: 0.25-mm (0.010-in) ID tubing (P/N 052306), two ferrule fittings (P/N 043276), and two 10-32 bolts (P/N 043275).

  • Page 93: Waste Line

    B.3.3 Waste Line Connect the waste line (P/N 054779) to the PDA-100 rear panel (see Figure B-1). Place the free end of the line in a waste container below the level of the lab bench on which the PDA-100 is installed.

  • Page 94: Relay/Ttl Control

    Stromversorgung wird mit Netzspannung betrieben. Der Service darf nur von qualifiziertem Personal durchgeführt werden. The PDA-100 Ship Kit includes a 12-pin connector for Relay and TTL connections (see Figure B-3). To attach individual wires and twisted pairs to the connector, strip the end of the wire(s), insert into the connector, and tighten the locking screw(s) with a screwdriver.
  • Page 95 B • Installation The table below describes the pin assignments for the Relay and TTL connectors. Connections 1 and 2 can be configured in the software to switch any low-voltage control. The switched current must be less than 200 mA and 42 V peak. Assignment Input Pin Function Relay 1 output...

  • Page 96: Dx-Lan Interface

    In order to communicate with a host computer running Dionex software, the DX-LAN interface must be installed. Connect an unshielded twisted- pair DX-LAN cable (P/N 960281) from the RJ-45 (10BASE-T) connector on the PDA-100 rear panel to a “combo” 10BASE-T Ethernet hub (P/N 056909). Installing the DX-LAN Cable 1.
  • Page 97 PEEK Flow Cell Outlet Tubing Assembly 043276 Ferrule 043275 060498 Flow Cell Windows Replacement Kit PDA-100 with Stainless Steel Flow Cell 056126 Stainless Steel Flow Cell (with heat exchangers) 060038 Stainless Steel Flow Cell Outlet Tubing Assembly 055902 Union Fitting...
  • Page 98 PDA-100 Photodiode Array Detector Doc. 031644-02 9/03...
  • Page 99 Index Numerics Backpressure line, 4-4, B-4 Bandwidth, 3-8 2D data, 1-1 Setting is too narrow, 4-5 3D data, 1-2, 3-5, 3-18 Baseline drift, 3-15 Bunch width selection, 3-24 Reducing, 3-20 Reducing data file size, 3-25 Baseline noise, 3-15, 5-2 Software support, 1-2, 3-5 Causes, 5-2 Step time selection, 3-25 Reducing, 3-16, 3-19 –...
  • Page 100 Conserving, 1-1, 3-18 Chromeleon software, 1-1 – 1-2 Drain tube, 2-12, B-5 3D option, 1-2, 3-5 DX-LAN connector, 2-12 Control of PDA-100, 3-5, 3-8 – 3-9 DX-LAN interface, 1-1 Leak alarm, 2-3 Cable specifications, B-8 See also Chromatography software Communication problem, 4-2, 4-10...
  • Page 101 Index Filter paddle, 2-4 – 2-5 Integrator, 4-11, B-5 Malfunction, 4-5 Isocratic analyses Positions, 2-5 Reference wavelength selection, 3-20, 4-7 Flow cell See Cells Front panel Illustration, 2-1 Lamp power supply, 2-8 LEDs described, 2-1 – 2-2 Lamps, 1-1 Fuses, 2-11, A-1 Calibration, 4-8 Replacing, 5-11 Deuterium LED flashing, 2-1...
  • Page 102 NOTES in manual, 1-7 Peak purity, 1-2 – 1-3, 3-5, 3-18 Peak resolution, 3-15 PeakNet 6 software, 1-1 3D option, 1-2, 3-5 Control of PDA-100, 3-5, 3-8 – 3-9 Offset level, 3-8 Leak alarm, 2-3 Operating parameters See also Chromatography software Optimization, 3-15 –...
  • Page 103 Correlation with data collection rate, 3-17 Preamp gain, 2-8 Definition, 3-16 Problems Effect on baseline noise, 3-16 Contacting Dionex for help, 4-1, 5-1 Guidelines for selecting, 3-16 – 3-17 See also Troubleshooting Inappropriate setting, 4-5 Pumping system requirements, 3-2 Run LED, 2-1...
  • Page 104 Elution time is slow, 4-3 Waste line, 2-12 Lamp does not light, 4-7 Installation, B-5 PDA-100 does not respond, 4-2 – 4-3 Wavelength, 3-8 Peak shape is poor, 4-12 Wavelength calibration, 1-1, 3-8, 4-9, 4-15 Peak size is wrong, 4-11...

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