Related Manuals for Mirus Ingenio EZporator MTR 51000
Summary of Contents for Mirus Ingenio EZporator MTR 51000
- Page 1 Ingenio® EZporator® Electroporation System Product User Manual MIR 51000 mirusbio.com RevB 121520...
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Page 2: Table Of Contents
Pulse Conditions for Common Cell Types ……………......16 Instrument Troubleshooting............17 Experimental Troubleshooting Low Electroporation Efficiency............. 18 High Cellular Toxicity..............19 Maintenance.................. 20 Warranty General Warranty................ 21 Out of Warranty Service.............. 22 Page 2 Mirus Bio www.mirusbio.com │ techsupport@mirusbio.com │ U.S. Toll Free: 844.MIRUSBIO (844.647.8724)│ Direct: +1.608.441.2852... -
Page 3: Technology Overview
The Ingenio® EZporator® was designed for use with Ingenio® Electroporation Kits and Solution. Use of electroporation solutions not manufactured by Mirus Bio may result in low transfection efficiency and high cellular cytotoxicity. Additionally, use of in- compatible electroporation solutions may cause damage to the instrument and will void the warranty. -
Page 4: System Components And Features
System and verify that the box contains the following: • EZporator® Pulse Generator (1) • EZporator® Cuvette Chamber (1) • Power cords (3) EZporator® Cuvette Chamber EZporator® Pulse Generator Power Cords Page 4 Mirus Bio www.mirusbio.com │ techsupport@mirusbio.com │ U.S. Toll Free: 844.MIRUSBIO (844.647.8724)│ Direct: +1.608.441.2852... -
Page 5: Technical Specifications
Not to be operated in conductive pollutants atmosphere Ordering Information: Catalog # Product MIR 51000 Ingenio® EZporator® Electroporation System MIR 51100 Ingenio® EZporator® Pulse Generator MIR 51200 Ingenio® EZporator® Cuvette Chamber Page 5 Mirus Bio www.mirusbio.com │ techsupport@mirusbio.com │ U.S. Toll Free: 844.MIRUSBIO (844.647.8724)│ Direct: +1.608.441.2852... -
Page 6: Safety Information-Please Read Carefully
ENVIRONMENTAL USAGE: This product should not be used in the presence of a flammable atmosphere such as an anesthetic mixture with air, oxygen or ni- trous oxide. Page 6 Mirus Bio www.mirusbio.com │ techsupport@mirusbio.com │ U.S. Toll Free: 844.MIRUSBIO (844.647.8724)│ Direct: +1.608.441.2852... - Page 7 • All service must be performed by Mirus Bio authorized personnel only. If there are any questions about the operation of this instrument, call Mirus Bio Technical Support at 888.530.0801 or +1.608.441.2852. Caution Notice This symbol indicates that caution is advised. Proper set-up, usage and PPE when using this instrument are required.
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Page 8: Set-Up Instructions Installation
When the instrument is initialized for the first time, the display will read “READY LV 400V.” Instrument Features and Controls: Voltage Adjust High Voltage Pulse Button Cuvette Chamber Output Page 8 Mirus Bio www.mirusbio.com │ techsupport@mirusbio.com │ U.S. Toll Free: 844.MIRUSBIO (844.647.8724)│ Direct: +1.608.441.2852... -
Page 9: Display And Status Options
The capacitors failed to charge properly. To reset this message, press the ‘Pulse’ button. Setpoint Too Low The entered voltage setpoint is below the normal operating specifications. No pulse has been applied. Page 9 Mirus Bio www.mirusbio.com │ techsupport@mirusbio.com │ U.S. Toll Free: 844.MIRUSBIO (844.647.8724)│ Direct: +1.608.441.2852... -
Page 10: Post-Pulse Display
Pulse button a second time before delivery of the pulse. Following the delivery of a pulse, press the Pulse button or rotate the Voltage Adjust knob to return to the ‘READY’ mode. Page 10 Mirus Bio www.mirusbio.com │ techsupport@mirusbio.com │ U.S. Toll Free: 844.MIRUSBIO (844.647.8724)│ Direct: +1.608.441.2852... -
Page 11: Ezporator® System Protocols
Following the delivery of a pulse, press the Pulse button or rotate the Voltage Adjust knob to return to the ‘READY’ mode. 10. Repeat steps 5-9 for additional samples. Page 11 Mirus Bio www.mirusbio.com │ techsupport@mirusbio.com │ U.S. Toll Free: 844.MIRUSBIO (844.647.8724)│ Direct: +1.608.441.2852... -
Page 12: Instructions For Use With Ingenio® Solution And Kits
7. After centrifugation, resuspend cells in Ingenio® Electroporation Solution us- ing the volume determined in step B3. (Cont. on next page) Page 12 Mirus Bio www.mirusbio.com │ techsupport@mirusbio.com │ U.S. Toll Free: 844.MIRUSBIO (844.647.8724)│ Direct: +1.608.441.2852... - Page 13 4. Incubate the electroporated cells in appropriate culture medium for 12-72 hours or as required. A culture medium change may be necessary for longer incubations. 5. Harvest cells and assay as required. Page 13 Mirus Bio www.mirusbio.com │ techsupport@mirusbio.com │ U.S. Toll Free: 844.MIRUSBIO (844.647.8724)│ Direct: +1.608.441.2852...
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Page 14: Optimization
Use siRNA that is highly pure, sterile and the correct sequence. Try siR- NA in the range of 250-750 nM (final concentration in cuvette) to determine the best siRNA concentration for electroporation. Page 14 Mirus Bio www.mirusbio.com │ techsupport@mirusbio.com │ U.S. Toll Free: 844.MIRUSBIO (844.647.8724)│ Direct: +1.608.441.2852... -
Page 15: Pulse Conditions
DNA at the specified voltages in 0.2 cm cuvettes (100 µl volume). Delivery efficiency and cell viability were determined by flow cytometry 48 hours post- electroporation: 130 V 150 V 110 V 170 V Page 15 Mirus Bio www.mirusbio.com │ techsupport@mirusbio.com │ U.S. Toll Free: 844.MIRUSBIO (844.647.8724)│ Direct: +1.608.441.2852... -
Page 16: Pulse Conditions For Common Cell Types
HeLa Hepa HepG2 HL-60 HUVEC Jurkat K562 MCF-7 NIH-3T3 NIKS PC-12 RAW 264.7 SH-SY5Y SK-BR-3 SK-N-MC THP-1 U-937 Vero Other 80-160 5-10 (Starting Point) 200-300 Page 16 Mirus Bio www.mirusbio.com │ techsupport@mirusbio.com │ U.S. Toll Free: 844.MIRUSBIO (844.647.8724)│ Direct: +1.608.441.2852... -
Page 17: Instrument Troubleshooting
Press the ‘Pulse’ button to reset this message. A very low line voltage or a brownout may be the cause. Verify that the outlet powering the EZporator® is adequately rated. If the message persists, contact Mirus Technical Support. Setpoint too low: The user has entered a setpoint that is outside of normal operating specifications. -
Page 18: Experimental Troubleshooting Low Electroporation Efficiency
® included for plasmid To assess delivery efficiency of plasmid DNA, use Mirus Bio Label IT Tracker™ Intracellular Nucleic Acid Localization Kit to label the target delivery plasmid or use Mirus Bio prelabeled Label IT® Plasmid Delivery Controls. -
Page 19: High Cellular Toxicity
DNA We recommend using Mirus Bio MiraCLEAN® Endotoxin Removal Kit (MIR 5900) for removal of any traces of endotoxin from your DNA preparation. Alternatively, use cesium chloride gradient or anion exchange purified DNA which contains levels of endotoxin that do not harm most cells. -
Page 20: Maintenance
20 mm, 2.5 A, 250 V type T (time delay) fuses. 4. Return the fuse and cap to the Pulse Generator module. Carefully turn right with the screwdriver to tighten. AC Input Fuse Power Button Page 20 Mirus Bio www.mirusbio.com │ techsupport@mirusbio.com │ U.S. Toll Free: 844.MIRUSBIO (844.647.8724)│ Direct: +1.608.441.2852... -
Page 21: Warranty
The Ingenio® EZporator® Electroporation System is warrantied for one year from the date of purchase. At its discretion, Mirus Bio will repair or replace the unit if it is found to be defective as to workmanship or materials. This warranty is considered void for any instrument which has been: 1. -
Page 22: Out Of Warranty Service
Shipment of your EZporator® Device to Mirus Bio should be prepaid. Your bill will include return shipment freight charg- es. Disassembly by the user is prohibited. Service should only be carried out by ex- perienced Mirus Bio technicians. - Page 23 Ingenio® EZporator® Electroporation System Product User Manual | Instructions for MIR 51000 Notes ©1996-2021 All rights reserved. Mirus Bio LLC. All trademarks are the property of their respective owners. For terms and conditions, visit www.mirusbio.com Page 23 Mirus Bio www.mirusbio.com │ techsupport@mirusbio.com │ U.S. Toll Free: 844.MIRUSBIO (844.647.8724)│ Direct: +1.608.441.2852...
- Page 24 Mirus Bio LLC 5602 Research Park Blvd., Ste. 210 Madison, WI 53719 USA U.S. Toll-free: 888.530.0801 Direct: +1.608.441.2852 5423-027 R2.0...
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