Omega Mag-Bind M2837-00 Product Manual page 16

M2839 Mag-Bind® Blood RNA 96 Protocol
20. Add 400 µL RNA Wash Buffer II. Pipet up and down 20 times or shake for 5 minutes to
mix.
21. Let sit at room temperature for 5 minutes.
22. Place the plate on a magnetic separation device to magnetize the Mag-Bind®
Particles CNR. Let sit at room temperature until the Mag-Bind® Particles CNR are
completely cleared from solution.
23. Aspirate and discard the cleared supernatant. Do not disturb the Mag-Bind® Particles
CNR.
24. Remove the plate containing the Mag-Bind® Particles CNR from the magnetic
separation device.
25. Add 450 μL RNA Wash Buffer II to each well. Pipet up and down 20 times or shake for
2 minutes to mix thoroughly.
26. Place the plate on a magnetic separation device to magnetize the Mag-Bind®
Particles CNR. Let sit at room temperature until the Mag-Bind® Particles CNR are
completely cleared from solution.
27. Aspirate and discard the cleared supernatant. Do not disturb the Mag-Bind® Particles
CNR.
28. Leave the tube on the magnetic separation device for 10 minutes to air dry the Mag-
Bind® Particles CNR. Remove any residual liquid with a pipettor.
29. Add 100 µL DEPC Water. Pipet up and down 20 times or shake for 1 minute to mix
thoroughly.
30. Let sit at room temperature for 3 minutes.
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