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M2839 Mag-Bind® Blood RNA 96 Protocol
15. Aspirate and discard the cleared supernatant. Do not disturb the Mag-Bind® Particles
CNR.
16. Leave the tube on the magnetic separation device for 3 minutes to air dry the Mag-
Bind® Particles CNR. Remove any residual liquid with a pipettor.
17. Prepare the Mag-Bind® DNase I digestion mix as detailed in the table below:
Note: If total nucleic acid (DNA and RNA) is desired, skip Mag-Bind® DNase I
digestion steps (Steps 18-24) and proceed to Step 25 for isolating both DNA and
RNA.
Number of
Samples
1
4
10
96
* Volumes are calculated 10% extra to offset pipetting error.
Important Notes:
•
Mag-Bind® DNase I is very sensitive and prone to physical denaturation. Do not
vortex the Mag-Bind® DNase I mixture. Mix gently by shaking the plate.
•
Freshly prepare Mag-Bind® DNase I digestion mix right before RNA isolation.
•
All steps must be carried out at room temperature. Work quickly, but carefully.
18. Add 100 µL Mag-Bind® DNase I digestion mix. Pipet up and down 20 times or shake
gently for 2 minutes to mix.
Note: It is very important to remove any liquid drop from the wells before adding
the Mag-Bind® DNase I digestion mix. Mag-Bind® DNase I digestion mix must be used
immediately once it is prepared.
19. Let sit at room temperature for 10-15 minutes.
14
Mag-Bind® DNase I
Digestion Buffer
98 µL
431 µL*
1078 µL*
10.35 mL*
Mag-Bind® DNase I
2 µL
8.8 µL*
22 µL*
211 μL*
Total Volume
100 µL
439.8 µL
1100 µL
10.56 mL
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