BioLife ThawSTAR CFT2 Instructions For Use Manual

ThawSTAR
Automated Cell Thawing System

1. ThawSTAR Automated Cell Thawing System Function and Description

ThawSTAR Automated Cell Thawing System (Item No. AST-601) is designed to rapidly thaw live biological contents of 1.8-2.0 mL cryogenic
®
vials taken directly from liquid nitrogen (LN2) storage, a -80°C freezer or vials equilibrated and held at dry ice temperature (-78.5°C) in a
container such as the ThawSTAR
vial receives a customized thaw cycle based upon multiple built-in detection parameters. The intended result is a thermal profile equivalent
to a water bath thaw, but without the water, and comparable post-thaw cell recovery and viability.
LEDs
Base light

1.1 General Safety

ThawSTAR Automated Cell Thawing System is intended for the exclusive use by trained and experienced laboratory personnel.
1.1.1. Review instructions before installing, using or cleaning the unit.
1.1.2. DANGER: To prevent risk of severe or fatal electrical shock, use only the supplied AC power supply with cables for
the US, UK and EU packaged with the unit.
1.1.3. WARNING: Do not place near flammable materials. This unit is not designed to be explosion-proof or spark-proof.
1.1.4. WARNING: Avoid prolonged exposure to ultraviolet light such as sunlight or germicidal lamps unless covered by
light resistant plastic bag or a large piece of unbroken aluminum foil placed over the unit when not in use.
1.1.5. WARNING: Do not clean unit with ketone solvents; use recommended cleaning protocol in section 5.
1.1.6. CAUTION: Do not place near or immerse in water or other liquids.
1.1.7. WARNING: Removal of shell without authorization will void your limited warranty. Do not remove exterior shell of
the unit unless instructed to do so by an authorized BioLife representative.
1.1.8. WARNING: Unit has a covered mini-USB port on the bottom; do not insert any USB plug into this port or attempt to connect unit
with any other device, (eg. a computer), unless instructed to do so by an authorized BioLife representative.
1.1.9. WARNING: Do not use flag labels or labels that have a tab extending beyond the vial body or with folds or ridges with the
unit as these features may interfere with heat transfer or cause vial to become lodged in the unit.
1.1.10. CAUTION: The unit is designed for stationary indoor laboratory bench top use; outdoor "field" use or use on a
laboratory bench subject to strong vibrations such as near a centrifuge, shaker, or vortex mixer is not advised.
1.1.11. Ensure the unit is placed securely on a level surface during operation.
1.1.12. Recommended for use with standard 1.8 to 2.0 mL cryogenic vials containing cryopreserved cells; the ThawSTAR
system is optimized for 1.o mL volume of aqueous solution at a starting temperature of <- 70°C.
1.1.12.1. Ensure vials were frozen in an upright and vertical position and that frozen contents are at the bottom of the vial.
1.1.12.2. Vials should be gently inserted into the top opening of the unit;
should be inserted into the unit at any time.
1.1.13. CAUTION: Keep liquids out of the unit; ensure cryogenic vials have clean exteriors and are tightly capped to prevent leaks.
1.1.14. CAUTION: Do not touch the vial with hands or any other object until the thawing process is finished (after
cryogenic vial is inserted into the opening of the unit and the thawing process has been initiated).
1.1.15. Thawing time is typically 2-3 minutes from the time of vial insertion. Laboratory personnel are advised to be in close
proximity to the unit while in use so that vials may be immediately retrieved upon thaw completion.
1.1.16. Before moving the unit, ensure that any cryogenic vial has been removed from unit opening.
1.1.17. Keep unit opening covered while not in use.
1.1.18. HOT SURFACE WARNING: The heating elements inside the vial opening are heated while the unit is turned on. Avoid
inserting body parts such as fingers or any object except a 1.8 to 2.0 mL cryogenic vial into the vial opening at any time.
1.1.19. Ensure the power cord can be easily disconnected from the power supply when placing the unit on a laboratory bench.
CFT2
®
CFT2 Transporter (Item No. AST-602). Utilizing patent-pending STAR
®
Power
connection
Unit
opening
On/Off
switch
CAUTION: NO other objects (e.g. forceps)
Instructions for Use
adaptive sensing technology, each
™
Mini USB port (to be
used only by authorized
service personnel)
Speaker