1. Manuals
  2. Brands
  3. Gilson Manuals
  4. Water Pump
  5. 322
  6. User manual

Gilson 322 User Manual

Hplc pump
Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
User's Guide
322 HPLC Pump

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the 322 and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for Gilson 322

Summary of Contents for Gilson 322

  • Page 1 User's Guide 322 HPLC Pump...
  • Page 2 Trademarks All product and company names are trademarks™ or registered® trademarks of their respective holders. Use of the trademark(s) in this document does not imply any affiliation with or endorsements by the trademark holder(s).

  • Page 3: Table Of Contents

    CHAPTER 1 | Description unpacking Technical Specifications Customer Service INSTALLATION CHAPTER 2 | Electrical Connections Cabling for TRILuTION LC Control Hydraulic Connections OPERATION CHAPTER 3 | Front Panel and Startup Priming Pump Operating Parameters 322 HPLC PumP | uSER’S GuIDE...
  • Page 4 MAINTENANCE CHAPTER 4 | maintenance Overview Cleaning Pump Head Piston Seals Piston and Bellows Piston Rinsing Chamber Reassemble Pump Head Check Valves Filters maintenance Procedures Fuse Replacement TROUBLESHOOTING CHAPTER 5 | Troubleshooting Repair and Return Policies TABLE OF CONTENTS 322 HPLC PumP...
  • Page 5 REPLACEMENT PARTS APPENDIX A | 322 HPLC Pump Hydraulic Pump Heads Electrical miscellaneous (Tools, Power Cords, Cables, etc.) Service Parts COLUMN HOLDER APPENDIX B | Installation Part Numbers INJECTOR APPENDIX C | Injection Valve Holder Connection to Injector Part Numbers...

  • Page 7: Safety

    Failure to comply with these precautions or with specific warnings elsewhere in the user’s guide violates safety standards of design, manufacture, and intended use of the instrument. Gilson assumes no liability for the customer’s failure to comply with these requirements.

  • Page 8: Electronic And Hazard Symbols

    CAUTION indicates a potentially hazardous situation which, if not avoided, may result in minor CAUTION or moderate injury NOTICE indicates a potentially hazardous situation which, if not avoided, may result in NOTICE equipment damage SAFETY 322 HPLC PUmP...

  • Page 9: Lifting

    Be sure to use only replacement parts mentioned in the user’s guide. Do not repair the instrument or change parts not listed in the user’s guide. If it is necessary to replace parts not listed, please contact your local Gilson representative. 322 HPLC PUmP...

  • Page 11: Introduction

    Chapter 1 INtrODUCtION Chapter 1 | IN thIS Chapter ● Description ● unpacking ● Technical Specifications ● Customer Service 322 HPLC PumP | uSER’S GuIDE...

  • Page 12: Description

    Description The 322 HPLC Pump is a multi-solvent pump for analytical to semi-preparative high performance liquid chromatography (HPLC). It accommodates flow rates from 0.15 mL/min up to 30 mL/min at pressures up to 8700 psi (600 bar). It includes a built-in, high-pressure adjustable volume dynamic mixer (AVDm) allowing users to perform a binary gradient with a single unit.
  • Page 13 The pump heads must be dismounted for routine servicing purposes (for example, changing a piston seal). Figure 3 H2 Pump Head 322 HPLC PumP | uSER’S GuIDE...
  • Page 14 OUtLet FILter The outlet filter protects the injection valve and the column. The outlet filter can be replaced by the user. Figure 6 Purge Valve and Outlet Filter InTRoDuCTIon 322 HPLC PumP...
  • Page 15 Front Panel ON/OFF The 322 Pump must be controlled by external software, such as TRILuTIon LC. The 322 Pump has a front panel to control power or POWER indicate errors. REMOTE Rear Panel Figure 7 322 HPLC Pump Standby Panel...

  • Page 16: Unpacking

    It is necessary for two people to lift the 322 Pump out of the box, using the straps provided. The 322 Pump weighs approximately 26 kg (57 lbs.). To avoid personal injury and for general safety, if moving or lifting the system, always get another person to assist you.

  • Page 17: Technical Specifications

    Technical Specifications Please be aware of the following before operating the pump. Changes or modifications to the pump not expressly approved by Gilson could void the NOTICE warranty. This instrument complies with part 15 of the FCC Rules. operation is subject to the following two conditions: (1) This instrument may not cause harmful interference, and (2) this instrument must accept any interference received, including interference that may cause undesired operation.

  • Page 18: Customer Service

    If you need assistance, please contact your local Gilson representative. Specific contact information can be found at www.gilson.com. To help us serve you quickly and efficiently, please refer to Repair and Return Policies on page 49.

  • Page 19: Chapter 2 | Installation

    TRILuTION® LC Software is the recommended controller for the pump. The pump is delivered with the main hydraulic components installed. Solvent bottles can be placed in the removable trays atop the pump, on the bench, or on the floor. 322 HPLC PumP | uSER’S GuIDE...
  • Page 20 Figure 11 The GSIOC ID (0–9) is set mechanically for the GSIOC ID (0–9) 322 Pump using the SW1 (left) selector on the rear panel. BaUD rate The baud rate (9600, 19200, or External) is set mechanically using the SW2 (right) selector on the rear panel.

  • Page 21: Cabling For Trilution Lc Control

    Plug one end of a GSIOC cable into the port marked GSIOC FROm CONTROLLER. Plug the other end into the GSIOC port of an interface module (or other controlling device, such as a Gilson liquid handler), and then connect an RS-232 cable from the interface module (or other controlling device) to the computer.
  • Page 22 The other end of the tubing goes to the drain (or a suitable receptacle) via the drain tubing exit port. Installation is completed by replacing the drip tray. Figure 15 Drip Tray Diagram INSTaLLaTION INSTaLLaTION 322 HPLC PumP 322 HPLC PumP...
  • Page 23 For details about installing the piston rinse plumbing and the piston rinsing procedure, refer to Piston Rinsing Procedure on page 28. 322 HPLC PumP 322 HPLC PumP | uSER’S GuIDE | uSER’S GuIDE...
  • Page 24 0.2–0.3 0.7–2 0.4–1.0 2.1–5 1.1–2.2 >5 When the dynamic mixer is operating the indicator (green LED) lights up. The AVDM may only CAUTION be adjusted when the pump is stopped and depressurized. INSTaLLaTION INSTaLLaTION 322 HPLC PumP 322 HPLC PumP...

  • Page 25: Operation

    IN thIS Chapter ● Front Panel and Startup ● Priming ● Pump Operating Parameters TRILuTION® LC Software provides software control of the pump for setup and operation. Refer to the TRILUTION® LC Software User’s Guide for more information. 322 HPLC PumP | uSER’S GuIDE...

  • Page 26: Front Panel And Startup

    10. Turn the knob on the purge valve all the way to the right (clockwise) to close the outlet to waste. Depending on your solvents, ensure that the rinse plumbing kit is installed. Refer to Piston Rinsing Procedure on page 28. OPERaTION 322 HPLC PumP...
  • Page 27 10. Repeat steps 5–9 for any additional mobile phase instruments. This will ensure that each pump head is primed individually. Stop Pump on the manual Control window. 11. Stop the pump by selecting 12. Close the purge valve. 322 HPLC PumP | uSER’S GuIDE...
  • Page 28 Over time the buffer concentrate will dilute into the water bottle via entropy and laminar flow. NOTICE To prevent sediment build-up in the piston chamber, change the water and clean the bottle. OPERaTION 322 HPLC PumP...

  • Page 29: Pump Operating Parameters

    The inlet pressure can be adjusted from 0–50 mPa. Compressibility Compressibility is a compensation parameter for solvent compressibility. The compressibility can be adjusted from 0–2000 mbar . The standard compressibility values are 34 for water, 162 for methanol, and 180 for acetonitrile. 322 HPLC PumP | uSER’S GuIDE...

  • Page 31: Maintenance

    ● Cleaning check valves and filters (clean outlet filter after changing piston seal) ● Replacing parts subject to wear and tear in each pump head: piston seal, check valves, and piston assembly ● Run-in the pump head and/or seal 322 HPLC PUMP | USER’S GUIDE...

  • Page 32: Maintenance Overview

    Refer to the Material Safety Data Sheets for the solvents used. Cleaning Keep the pump clean for peak performance. Always turn the power off to the pump before cleaning. Wipe the pump with a soft cloth dampened with a mild detergent and disinfect as needed. MAInTEnAnCE 322 HPLC PUMP...

  • Page 33: Pump Head

    Run. 9. Select 10. Wait for the pistons to fully retract. Repeat steps 1-10 to mount the pump heads, but select TRUE for the MountHeads property in the NOTE ServiceHeads command. 322 HPLC PUMP | USER’S GUIDE...
  • Page 34 5 mm Allen wrench. Support the pump head with your other hand while loosening the screws, alternating equally between the two. Moderate force may be required to remove the pump head. Figure 23 Loosening Pump Head Screws MAInTEnAnCE 322 HPLC PUMP...
  • Page 35 1. Undo the two retaining screws using a 3 mm Allen wrench (part number 4320302). Figure 25 Initial Steps for Pump Head Disassembly 2. Remove the white cap and retaining screws. Figure 26 Remove the White Cap and Retaining Screws 322 HPLC PUMP | USER’S GUIDE...
  • Page 36 3. Pull the piston and bellows out of the body of the pump head. Figure 27 Pull the Piston and Bellows Out of Pump Head 4. Pull apart the two halves of the pump head using a slight twisting motion. MAInTEnAnCE 322 HPLC PUMP...

  • Page 37: Piston Seals

    Figure 29 cloth or an air line. Piston Seal Removal 3. Push a new seal carefully into the recess. 4. Initialize and run-in the new seal(s) as described in Run-In Procedure on page 45. 322 HPLC PUMP | USER’S GUIDE...

  • Page 38: Piston And Bellows

    1. Insert the bellows tool (part number 38013235) into the open end of the bellows. This tool helps ensure that the orifice at the end of the bellows remains open while you work. Figure 35 Figure 34 Opening the Orifice Inserting the Tool MAInTEnAnCE 322 HPLC PUMP...

  • Page 39: Piston Rinsing Chamber

    Metallic Ring Collar Figure 38 Metallic Ring and Piston Collar Seating Piston Rinsing Chamber Change the rinsing liquid at least once a week. For details, refer to Piston Rinsing Procedure on page 28. 322 HPLC PUMP | USER’S GUIDE...

  • Page 40: Reassemble Pump Head

    (do not overtighten the screws). Figure 41 Figure 42 Refitting the White Cap Tightening the Screws Do not turn the piston after the head is reassembled, because it is possible to damage the NOTICE bellows. MAInTEnAnCE 322 HPLC PUMP...

  • Page 41: Check Valves

    This also applies to the check valve cartridges, which have different forms. The check valves must not be disassembled into sub-components. no check valve sub-component is available from Gilson. Cleaning a Check Valve This procedure is carried out with the check valves installed: 1.
  • Page 42 14 mm wrench until there is contact, then tighten the connector by turning it a further 30° in the clockwise direction. If leakage is observed, tighten the connector progressively until the leakage stops. 7. Run the pump and perform the Leak Test Procedure on page MAInTEnAnCE 322 HPLC PUMP...

  • Page 43: Filters

    Purge Valve and Outlet Filter CLeaNING the OUtLet FILter 1. Remove the filter from the pump. 2. Separate the two halves of the filter casing using two 11 mm wrenches. Figure 47 Separate the Filter Casing 322 HPLC PUMP | USER’S GUIDE...
  • Page 44 (downstream) half of the casing to ensure that the cartridge is properly seated before reassembling the filter casing. 7. Refit the filter to the pump and re-prime the system, checking for leaks. MAInTEnAnCE 322 HPLC PUMP...

  • Page 45: Maintenance Procedures

    1. Run the pump unloaded for 1–2 minutes at 20% of nominal flow rate with methanol or isopropanol. 2. Run the pump at maximum operating pressure for 4–5 minutes. While running, check for any leaks. 3. Repeat the first step, but for 30 minutes. 322 HPLC PUMP | USER’S GUIDE...

  • Page 46: Fuse Replacement

    Fuse Replacement 4. Replace both fuses. Use only fuses with the rated current and specified type as listed on the rear panel of the instrument. 5. Insert the fuse drawer into its receptacle on the rear panel. MAInTEnAnCE 322 HPLC PUMP...

  • Page 47: Chapter 5 | Troubleshooting

    Check each component in the circuit, even if it is new. To receive notification of and to designate instructions for response to error states in TRILUTION LC, users must define parameters in the Method - error handling tab. 322 HPLC PUmP | USER’S GUIDE...
  • Page 48 Increase mixer volume Baseline noise, periodic pulses Air in the hydraulics Prime the pump, degas the solvent Faulty pressure module Contact your Gilson representative Contact your local Gilson representative or techsupport@gilson.com for assistance resolving NOTE problems described in this chapter. TROUbLESHOOTING...

  • Page 49: Repair And Return Policies

    Repair and Return Policies Before Calling Us Your local Gilson representative will be able to serve you more efficiently if you have the following information: ● Serial number and model number of the instruments involved ○ The serial number is located inside the door on the right side of the pump.

  • Page 51: Appendix A | Replacement Parts

    ● miscellaneous (Tools, Power Cords, Cables, etc.) ● Service Parts 322 HPLC Pump PART NUMBER DESCRIPTION 381032211 322-H1 Pump (110 and 220V), 2-solvent, 15 mL/min, 60 mPa 381032221 322-H2 Pump (110 and 220V), 2-solvent, 30 mL/min, 30 mPa Hydraulic Tubing and Fittings PART NUMBER...

  • Page 52: Pump Heads

    Chamber Body H1 3801320202 Chamber Body H2 3801320701 Rinsing Body Chamber H1 3801320801 Rinsing Body Chamber H2 38013296 Outlet Check Valve Chamber H1/H2 38013297 Inlet Check Valve Chamber H1 3801320204 Inlet Check Valve Chamber H2 REPLACEmEnT PARTS 322 HPLC PumP...

  • Page 53: Electrical

    Tubing clip, PP, for 4.8 mm, 3/16” for bottle tray 38073202 Drip pan, PP, with drain tubing (silicone, 4 x 6 mm, 1000 mm) MISCELLANEOUS (TOOLS, POWER CORDS, CABLES, ETC.) CONTINUED ON PAGE 54 322 HPLC PumP | uSER’S GuIDE...

  • Page 54: Service Parts

    Replacing these parts may require assistance from your technical service representative. PART NUMBER DESCRIPTION 38013242 Pressure module, 316 steel and PTFE, 60 mPa, 1.8–8.5 mL 38013278 Purge valve, 316 steel and PCTFE, 8 μL 360650 AVDm mixer with motor Assembly REPLACEmEnT PARTS 322 HPLC PumP...

  • Page 55: Appendix Bcolumn Holder

    The column holder attaches to the left side of the pump. Three pairs of column support, fitted with clips for different diameter columns, are provided in the kit. Column Support Retaining Knob Hole for Retaining Stud Retaining Knurled Column Stud Screw Support Figure 50 Column Holder 322 HPLC PumP | uSER’S GuIDE...

  • Page 56: Part Numbers

    38013203 Column holder, equipped for 2 to 20 mm ID column 380132661 Column holder clips, 1/4" (2 mm), 2/pk 380132662 Column holder clips, 1/2" (10 mm), 2/pk 380132663 Column holder clips, 1" (20 mm), 2/pk CoLumN HoLDER 322 HPLC PumP...

  • Page 57: Appendix C Injector

    Appendix C inJeCTOR Appendix C | in THiS CHApTeR ● Injection Valve Holder ● Connection to Injector ● Part Numbers 322 HPLC PumP | uSER’S GuIDE...

  • Page 58: Injection Valve Holder

    Valve holder with manual Rheodyne 7725i injection valve 38013206 Valve holder without valve 49931559 tubing, 316 steel, 0.25 x 1.6 x 550 mm, 27 µL (to injector, <10 mL/min) 49933509 tubing, 316 steel, 0.5 x 1.6 x 500 mm, 98 µL (to injector, >10 mL/min) INjECtoR 322 HPLC PumP...

  • Page 59: Appendix D | Reference Information

    PEEK™ The polymer surface will not leach metal ions into the eluent or extract metal-sensitive components from the sample. LIQUID CONTACT MATERIALS CONTINUED ON PAGE 60 322 HPLC PuMP | uSER’S GuIDE...
  • Page 60 PTFE, PEEK, FEP, ETFE and Titanium descriptions provided by Valco Instruments Co. Inc. (www.vici.com) PCTFE description provided by Fluorotherm (www.fluorotherm.com) Stainless Steel, Type 316L description provided by new England Small Tube Corporation (www.nesmalltube.com) Ruby/Sapphire description provided by Ceramaret SA (www.ceramaret.ch) REFEREnCE InFoRMATIon 322 HPLC PuMP...

  • Page 61: Solvent Miscibility

    Refer to the table below when selecting solvents. For some solvents, lower toxicity alternatives are indicated [(1), (2), (3)], as follows: (1) Dichloromethane (2) Tetrahydrofuran (3) Ethanol 322 HPLC PuMP | uSER’S GuIDE...
  • Page 64 LT801384-04...

This manual is also suitable for:

322-h1322-h2381032211381032221

Table of Contents