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BIO X Autocalibration Kit
Installation and Quick-start Guide
The Autocalibration Kit enables Automatic Bed Leveling (ABL) and automatic X, Y and Z
calibration. These features are available on HeartOS software version 1.7 and above.
The BIO X autocalibration removes the potential for user error when calibrating the tip of the
nozzle relative to the printing surface or to another nozzle. It does this by probing and
aligning all printhead nozzle tips in all axes (X, Y and Z), and includes ABL.
ABL measures the print bed surface and adjusts all movements to follow its tilt and contours
to improve the quality of bioprinted structures. ABL compensates for any height differences
1
present in a print bed or printing surface. After a brief calibration procedure, the print bed
adjusts the Z height while running the bioprinting protocol, ensuring the construct is level
relative to the printing surface. ABL requires a surface probe to automatically measure the
print bed's height at various points.
Virginia, U.S.
Kyoto, Japan
Gothenburg, Sweden
Boston, U.S.
46-29 Yoshida-Shimo Adachi-
Arvid Wallgrens Backe 20,
2000 Kraft Dr., Suite 2125
100 Franklin St.，
cho, Sakyo-ku, Kyoto
Blacksburg, VA 24060
Gothenburg, 41346
Boston, MA 02110
www.cellink.com | +1 (833) CELLINK
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Summary of Contents for Cellink BIO X Autocalibration Kit
- Page 1 BIO X Autocalibration Kit Installation and Quick-start Guide The Autocalibration Kit enables Automatic Bed Leveling (ABL) and automatic X, Y and Z calibration. These features are available on HeartOS software version 1.7 and above. The BIO X autocalibration removes the potential for user error when calibrating the tip of the nozzle relative to the printing surface or to another nozzle.
- Page 2 BIO X Autocalibration Kit (Barcode: D16110021178) Part Quantity/Barcode Surface probe 000000020628 Surface probe stabilizer 000000020627 Surface probe magnet D16110021355 Z-station base 000000020625 Z-station plate 000000020624 XY printhead calibration lever 000000020629 M4 screw 000000020419 Virginia, U.S. Kyoto, Japan Gothenburg, Sweden Boston, U.S.
- Page 3 Before installing the BIO X Autocalibration Kit NOTE: Before installing the BIO X Autocalibration Kit, make sure that your BIO X is running HeartOS 1.7 or above. If the HeartOS software installed on the BIO X is version 1.6 or older, your system will not be compatible with the Autocalibration Kit.
- Page 4 NOTE: If any endstop components are misaligned, absent or not activating, contact the CELLINK Support team at support@cellink.com. Virginia, U.S. Kyoto, Japan Gothenburg, Sweden Boston, U.S. 46-29 Yoshida-Shimo Adachi- Arvid Wallgrens Backe 20, 2000 Kraft Dr., Suite 2125 100 Franklin St.，...
- Page 5 Installation of BIO X Autocalibration Kit Step 1: Installing the surface probe station 1. Turn the BIO X off and remove all printheads. 2. Unscrew the bottom mechanical endstop screw of the second printhead, labeled by the green circle in the diagram.
- Page 6 Virginia, U.S. Kyoto, Japan Gothenburg, Sweden Boston, U.S. 46-29 Yoshida-Shimo Adachi- Arvid Wallgrens Backe 20, 2000 Kraft Dr., Suite 2125 100 Franklin St.， cho, Sakyo-ku, Kyoto Blacksburg, VA 24060 Gothenburg, 41346 Boston, MA 02110 www.cellink.com | +1 (833) CELLINK...
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Page 7: Machine Calibration
Turn the screw to secure the cartridges in place. Virginia, U.S. Kyoto, Japan Gothenburg, Sweden Boston, U.S. 46-29 Yoshida-Shimo Adachi- Arvid Wallgrens Backe 20, 2000 Kraft Dr., Suite 2125 100 Franklin St.， cho, Sakyo-ku, Kyoto Blacksburg, VA 24060 Gothenburg, 41346 Boston, MA 02110 www.cellink.com | +1 (833) CELLINK... - Page 8 Virginia, U.S. Kyoto, Japan Gothenburg, Sweden Boston, U.S. 46-29 Yoshida-Shimo Adachi- Arvid Wallgrens Backe 20, 2000 Kraft Dr., Suite 2125 100 Franklin St.， cho, Sakyo-ku, Kyoto Blacksburg, VA 24060 Gothenburg, 41346 Boston, MA 02110 www.cellink.com | +1 (833) CELLINK...
- Page 9 1. Make sure the surface probe is attached. Virginia, U.S. Kyoto, Japan Gothenburg, Sweden Boston, U.S. 46-29 Yoshida-Shimo Adachi- Arvid Wallgrens Backe 20, 2000 Kraft Dr., Suite 2125 100 Franklin St.， cho, Sakyo-ku, Kyoto Blacksburg, VA 24060 Gothenburg, 41346 Boston, MA 02110 www.cellink.com | +1 (833) CELLINK...
- Page 10 Virginia, U.S. Kyoto, Japan Gothenburg, Sweden Boston, U.S. 46-29 Yoshida-Shimo Adachi- Arvid Wallgrens Backe 20, 2000 Kraft Dr., Suite 2125 100 Franklin St.， cho, Sakyo-ku, Kyoto Blacksburg, VA 24060 Gothenburg, 41346 Boston, MA 02110 www.cellink.com | +1 (833) CELLINK...
- Page 11 Virginia, U.S. Kyoto, Japan Gothenburg, Sweden Boston, U.S. 46-29 Yoshida-Shimo Adachi- Arvid Wallgrens Backe 20, 2000 Kraft Dr., Suite 2125 100 Franklin St.， cho, Sakyo-ku, Kyoto Blacksburg, VA 24060 Gothenburg, 41346 Boston, MA 02110 www.cellink.com | +1 (833) CELLINK...
- Page 12 5. Once in the print page, press Calibrate and select Automatic bed-leveling. Virginia, U.S. Kyoto, Japan Gothenburg, Sweden Boston, U.S. 46-29 Yoshida-Shimo Adachi- Arvid Wallgrens Backe 20, 2000 Kraft Dr., Suite 2125 100 Franklin St.， cho, Sakyo-ku, Kyoto Blacksburg, VA 24060 Gothenburg, 41346 Boston, MA 02110 www.cellink.com | +1 (833) CELLINK...
- Page 13 8. Use automatic calibration or remove the probe for manual calibration. Virginia, U.S. Kyoto, Japan Gothenburg, Sweden Boston, U.S. 46-29 Yoshida-Shimo Adachi- Arvid Wallgrens Backe 20, 2000 Kraft Dr., Suite 2125 100 Franklin St.， cho, Sakyo-ku, Kyoto Blacksburg, VA 24060 Gothenburg, 41346 Boston, MA 02110 www.cellink.com | +1 (833) CELLINK...
- Page 14 4. Press Calibrate in the print page. Select Automatic calibration. Virginia, U.S. Kyoto, Japan Gothenburg, Sweden Boston, U.S. 46-29 Yoshida-Shimo Adachi- Arvid Wallgrens Backe 20, 2000 Kraft Dr., Suite 2125 100 Franklin St.， cho, Sakyo-ku, Kyoto Blacksburg, VA 24060 Gothenburg, 41346 Boston, MA 02110 www.cellink.com | +1 (833) CELLINK...
- Page 15 8. Select Start to begin the calibration process. Virginia, U.S. Kyoto, Japan Gothenburg, Sweden Boston, U.S. 46-29 Yoshida-Shimo Adachi- Arvid Wallgrens Backe 20, 2000 Kraft Dr., Suite 2125 100 Franklin St.， cho, Sakyo-ku, Kyoto Blacksburg, VA 24060 Gothenburg, 41346 Boston, MA 02110 www.cellink.com | +1 (833) CELLINK...
- Page 16 Virginia, U.S. Kyoto, Japan Gothenburg, Sweden Boston, U.S. 46-29 Yoshida-Shimo Adachi- Arvid Wallgrens Backe 20, 2000 Kraft Dr., Suite 2125 100 Franklin St.， cho, Sakyo-ku, Kyoto Blacksburg, VA 24060 Gothenburg, 41346 Boston, MA 02110 www.cellink.com | +1 (833) CELLINK...
- Page 17 Virginia, U.S. Kyoto, Japan Gothenburg, Sweden Boston, U.S. 46-29 Yoshida-Shimo Adachi- Arvid Wallgrens Backe 20, 2000 Kraft Dr., Suite 2125 100 Franklin St.， cho, Sakyo-ku, Kyoto Blacksburg, VA 24060 Gothenburg, 41346 Boston, MA 02110 www.cellink.com | +1 (833) CELLINK...
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