Cellink BIO X 10-mL User Manual
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Cellink BIO X 10-mL User Manual

Pneumatic printhead
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BIO X 10-mL Pneumatic Printhead
User Manual

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Summary of Contents for Cellink BIO X 10-mL

  • Page 1 BIO X 10-mL Pneumatic Printhead User Manual...

  • Page 3: Table Of Contents

    Contents 1.Package contents 6.Frequently asked questions 2.Technical specifications 7.Maintenance 3.Safety information Support information 3.1 BIO X system warnings 3.2 Pneumatic Printhead warnings 4.Getting started 4.1 Unpacking and installation 4.2 Raising and lowering the printhead 4.3 Loading the bioink cartridge 4.4 Your first bioprint 4.5 Removing the printhead 4.6 Printing optimization 5.Relevant G-code commands...

  • Page 5: Package Contents

    Package contents...
  • Page 6 01 Package contents Item Part number Quantity BIO X 10 mL Pneumatic Printhead 000000020356 Air adapter connector 10 mL 000000610041...

  • Page 7: Technical Specifications

    Technical specifications...
  • Page 8 02 Technical specifications • Compatible with 10-mL plastic cartridge. • Dimensions (height x width x depth): • With screw: 63.5 x 30.5 x 51 mm. • Without screw: 63.5 x 30.5 x 46.2 mm. • Weight: 102 g. • Maximum recommended pressure: 700 kPa. •...
  • Page 9 02 Technical specifications • Running temperature (in recommended conditions): 26-32 degrees. • Heating capacity and rate: • Total heating range: 30-65 degrees. • Heating rate: Room temperature-40 degrees (10 minutes). • Heating rate: 40-65 degrees (12 minutes). • Cooling capacity: N/A. •...
  • Page 10 02 Technical specifications *All measurements are in mm. Figure 1: Theoretical maximum build volume as shown from the top and side view using the Pneumatic Printhead. Render assumes a nozzle length of zero. Exact build volume depends on cartridge position in printhead and nozzle/ needle used.
  • Page 11 02 Technical specifications Table 1: Theoretical build volume based on tips of common lengths. Actual volume can vary based on build surface thickness, size, shape and level, as well as cartridge position and tightness of tip. X, Y and Z are measured from the front left corner of the printbed. Tip Type Tip Length Nozzle/Needle Length...

  • Page 13: Safety Information

    Safety information...

  • Page 14: Bio X System Warnings

    03 Safety information 3.1 BIO X system warnings • Please consult the BIO X manual for BIO X-specific and general warnings and safety procedures. 3.2 10-mL Pneumatic Printhead warnings • Do not clean the printhead by submerging it in liquid or using excessive spraying. Liquid inside the printhead can damage the circuitry and motor.

  • Page 15: Getting Started

    Getting started...

  • Page 16: Unpacking And Installation

    04 Getting started 4.1 Unpacking and installation NOTE: The printing parameters displayed on this manual might not be accurate for your specific protocol. For recommended parameters please consult the documentation for the bioink being used. 1. Open the package. Remove the 10-mL Pneumatic Printhead. 2.

  • Page 17: Raising And Lowering The Printhead

    04 Getting started 4.2 Raising and lowering the printhead 1. On the user interface, navigate to the Utilities menu and then go to the Tools submenu. 2. Lower the desired printhead mount into the active or loading position by pressing the down arrow (Figure 3). 3.

  • Page 18: Loading The Bioink Cartridge

    04 Getting started 4.3 Loading the bioink cartridge 1. Make sure that the printhead is installed and in the active or loading position (Section 4.1 and 4.2) (Figure 2). 2. Fill a cartridge with bioink or obtain a prefilled cartridge. Remove the bottom cap if using a prefilled cartridge (Figure 4A).
  • Page 19 04 Getting started 4. Load the cartridge into the printhead from the top (Figure 5B). Tighten the locking screw. To make this step easier, we recommend placing the printhead in the active position (lowered) as explained in the Raising and lowering the printhead section (page 13) (Figure 5A).

  • Page 20: Your First Bioprint

    04 Getting started 4.4 Your first bioprint 1. Prepare the printhead and load the bioink as detailed in Section 4.3. 2. Select Bioprint from the Start menu (Figure 6). Figure 6: Select Bioprint on the Start menu and proceed to print setup.
  • Page 21 04 Getting started 3. Select an STL file (filename.stl) or G-code file (filename.gcode) from the Model menu (Figure 7). Proceed to the next menu by selecting Surface along the bottom of the screen. Figure 7: Selecting an STL file from the Model menu.
  • Page 22 04 Getting started 4. Select a surface to print on (e.g., a Petri dish) and proceed to the next menu (Figure 8). Figure 8: Select a print surface from the Surface menu.
  • Page 23 04 Getting started 5. Select printhead positions that have been set up. Ensure that Pneumatic 10-mL is selected under the printhead type (Figure 9). 6. Enter your printhead parameters (Figure 9) and proceed to the Layers menu. Parameters needed for the Pneumatic Printhead include: •...
  • Page 24 04 Getting started 7. Assign the enabled printhead to the respective layer characteristics and proceed to the next menu (Figure 10). Figure 10: Layer menu. You can assign toolheads to respective print areas like perimeter, infill and support. You can select the infill pattern and density and preview the layers.
  • Page 25 04 Getting started 8. Prime the nozzle/needle and test bioink flow by using the Drop button next to the pressure setting (Figure 11). Press Print to proceed to the calibration page. Figure 11: The Print menu is where you can preview your print parameters and test the extrusion rate.
  • Page 26 04 Getting started 9. Select Calibrate to calibrate the system to the desired start position (Figure 12). Bring the tip of the nozzle/needle to your desired starting position. We recommend making sure that the tip touches the surface of your printing substrate. 10.

  • Page 27: Removing The Printhead

    04 Getting started 4.5 Removing the Pneumatic Printhead 1. Move the printhead to the active or loading position from the Tools submenu under the Utilities menu. See Section 4.2 for instructions. 2. Loosen the screw and remove the cartridge from the top. 3.
  • Page 28 04 Getting started Figure 13: Removing the Pneumatic Printhead from the BIO X.

  • Page 29: Printing Optimization

    04 Getting started 4.6 Pneumatic Printhead printing optimization The Pneumatic Printhead uses compressed air to extrude bioink through a nozzle or needle with diameters between 50 and 600 micrometers. As a result, you can increase or decrease the air pressure to control the filament dispensing rate. Parameters that affect the deposited material’s diameter include nozzle diameter, translation speed, layer height, bioink chemistry and thermal environment.
  • Page 30 04 Getting started Preflow delays can be set to ensure continuous filaments at the beginning of each layer and various starts and stops. This is due to the mechanical compression of the bioink in the cartridge, resulting in a delay in flow. The preflow delay setting will pause printhead movement after the air pressure valve is opened at the designated time.

  • Page 31: Relevant G-Code Commands

    Relevant G-code commands...
  • Page 32 05 Relevant G-code commands Commands Description G1 Xnnn Ynnn Ennn Fnnn When used in combination with the G90 command, which defines absolute coordinates, G1 is the absolute move command. The values for the X and Y parameters are the coordinates (in mm) directing where to move.
  • Page 33 05 Relevant G-code commands G7 Znnn Ennn Fnnn When used in combination with the G90 command, which defines absolute coordinates, G7 is the relative move command. The value for the Z parameter is the coordinate (in mm) directing where to move relative to the current position. E tells the BIO X to open the valve for extrusion.

  • Page 35: Frequently Asked Questions

    Frequently asked questions...
  • Page 36 06 Frequently asked questions • Can I print temperature-sensitive materials using the Pneumatic Printhead? The Pneumatic Printhead will warm up during normal operation due to the embedded chips and other electronic components. If precise temperature control is needed, we recommend using the Temperature-controlled Printhead in a room with an ambient temperature between 20 and 23 degrees Celsius.
  • Page 37 06 Frequently asked questions enough to pass through the needle or nozzle without clogging it. If using a thermosensitive bioink, check to see if it has gelled at the tip. If it has, consider using the Temperature- controlled Printhead to prevent thermal gelation. •...

  • Page 39: Maintenance

    Maintenance...
  • Page 40 07 Maintenance • Regularly clean the printhead with a damp cloth to remove any dust or debris.

  • Page 41: Support Information

    Appendix A: Consumables— needles and nozzles...
  • Page 42 Appendix A: Consumables- needles and nozzles Table 2: Compatible needles Part Number Type Color Gauge Length (inch) NZ5180505001 Needle Green NZ6200255001 Needle Pink 0.25 NZ6200505001 Needle Pink 0.50 NZ5201005001 Needle Pink 1.00 NZ6210255001 Needle Purple 0.25 NZ6210505001 Needle Purple 0.50 NZ5211005001 Needle Purple...
  • Page 43 Appendix A: Consumables- needles and nozzles NZ7220505001 Needle Steel 0.50 NZ6230255001 Needle Orange 0.25 NZ6230505001 Needle Orange 0.50 NZ6231005001 Needle Orange 1.00 NZ6250255001 Needle 0.25 NZ6250505001 Needle 0.50 NZ5251005001 Needle 1.00 NZ6270255001 Needle Clear 0.25 NZ6270505001 Needle Clear 0.50 NZ5271005001 Needle Clear 1.00...
  • Page 44 Appendix A: Consumables- needles and nozzles NZ5320255001 Needle Yellow 0.25 NZ5320505001 Needle Yellow 0.50 NZ5340255001 Needle Green 0.25 NZ5340505001 Needle Green 0.50 Table 3: Compatible conical nozzles Part Number Type Color Gauge Length (inch) NZ4180005001 Conical Green 1.25 NZ4200005001 Conical Pink 1.25 NZ3220005002...
  • Page 45 Appendix A: Consumables- needles and nozzles Table 4: Compatible precision conical nozzle Part Number Type Color Gauge Length (inch) NZ2210000801 0.75 Micron-s Conical Light Blue NZ2230000801 Micron-s Conical Purple 0.75 NZ2250000801 Micron-s Conical White 0.75 NZ2270000801 Micron-s Conical 0.75 NZ2300000801 Micron-s Conical Black 0.75...
  • Page 46 Appendix A: Consumables- needles and nozzles Table 5: Compatible cartridges and cartridge accessories Part Number Description Quantity CSC011011102 Empty Cartridges 10cc with End and Tip Caps CSC011011502 Empty Cartridges 10cc with End and Tip Caps 000000020878 10ml End Caps OH000000010 Female/female Luer lock adapter OH000000050 Female/female Luer lock adapter...
  • Page 47 Support information • Official site: www.cellink.com • Contact: support@cellink.com • Contact: sales@cellink.com • Web store: www.cellink.com/store Store Sales Support Official site...
  • Page 48 www.cellink.com...

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