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Tubing selection
The choice of tubing depends on the requirements for speed and precision of dosing. The
larger the inner diameter (ID) of tubing, the faster but less precise is the dispensing.
Precision in the table below is expressed as coefficient of variation (CV) and describes
the scattering range of the dispenses relative to the mean.
Tubing inner diameter
Dosing volume at CV <1 %
Speed range (ml/min)
To dispense with CV <1
and read the appropriate tubing ID (e.g. 3 mm). The 8 mm tubing is only recommended for PUMP
modes.
Loading the tubing in the pump head
Always switch off the DOSE IT when loading the tubing and when manipulating the open
pump head.
• Open the cover of the pump head. The adjustment of the screws (1) on both sides of the pump
head depends on the tubing inner diameter (ID). Adjust the 1.6 marking of the clamps as
follows:
• Place the tubing into the pump head. Make sure that the tubing runs over the rollers and
centrally through the two V-shaped clamps. Carefully close the pump head.
• Connect the dispensing and aspiration tubes with tube collar, if required.
Adapt a dispensing program
In the APPLICATION window the first programs are already pre-defined with default values.
• Select a program to be defined using the arrow keys and press PARAMETER in order to adjust
the program.
• Using the arrow keys, select a parameter you wish to change. Press CHANGE and follow the
information on the screen.
• Use the DISPENSE type for dosing and the PUMP mode for continuously pumping of a liquid.
• The CUSTOMIZE type allows to define a program consisting of up to 20 single steps, see OI.
The predefined programs DENSITY GRADIENT, DISH COATING and DILUTION 1 TO 10 are
examples of this.
Run a dispensing program
• Select a previously defined program and press START.
• Put the tubing end with the aspiration tube in the liquid and hold the PRIME key pressed until
the tubing is completely filled without bubbles.
• The first time you start a program or use a tubing, carry out a calibration of the dosing volume
by pressing CALIBRATE and follow the instructions on the screen. To reduce the measurement
error, press repeatedly DOSING and dispense up to ten samples. Press CONTINUE, enter the
measured volume and press CHANGE. Press CONTINUE to end the calibration.
• Press START. While dispensing, you can PAUSE and adjust the program at any time.
2/4
1 mm
2 mm
>0.5 ml
>1 ml
0.6
–
5
2
2.1
%
,
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a
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Tubing ID
1.6 marking of the clamps
1 mm
between 3.2 and 4.8 scale values
2 mm
matches the 4.8 value on the scale (2)
3 mm
matches the 6.4 value on the scale (2)
4
–
8
m
m
as low as possible, completely open
3 mm
>3 ml
–
2
0
3
4.8
–
4
7
5
h t
e
d
e
i s
e r
d
d
s o
n i
g
o v
u l
4 mm
6 mm
>7 ml
>15 ml
8.4
–
8
3
7
16
–
1
m
e
(
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. g
5
m
, l
. i
. e
>
3
m
171254_V02_QSG_DOSE_IT_EN.docx
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