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Related Manuals for Genetix ClonePix FL
Summary of Contents for Genetix ClonePix FL
- Page 1 ClonePix Robot Manual 07MAN1054.A2 Effective Date: 27-May-11...
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Page 2: Table Of Contents
PC Connections ......................... 9 Filter options ..........................10 Robot Enclosure ....................11 Installation ......................12 Layout of ClonePix FL and Ancillary Equipment ..............12 Source and Destination Plate Stackers ................. 13 Connecting the Wash Bottles ....................14 Connecting the Ethanol Bottles ..................... 14 Camera Alignment ........................ - Page 3 GENETIX > CLONEPIX FL ROBOT MANUAL General Maintenance ..................21 Regular Maintenance ......................21 General Precautions ........................ 22 Cleaning Procedures ....................... 22 Removable Parts ......................... 22 Cleaning of Instrument Interior .................... 24 Cleaning of System Fluid (Sterile Water) Supply ..............24 Incoming Compressed Air Supply ..................
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Page 4: Table Of Figures
Table of Figures Figure 1: ClonePix FL electrical connections ................8 Figure 2: ClonePix FL PC connections ..................9 Figure 3: ClonePix FL layout and dimensions ................ 12 Figure 4: Stacker orientation ....................13 Figure 5: Stackers and cassettes ..................... 14 Figure 6: Connecting the wash bottles .................. -
Page 5: Introduction To Clonepix Fl
For detailed information about the software please see the ClonePix FL Software Application Manual. Note: ClonePix FL is strictly for research use only and is not intended or recommended for the diagnosis of disease in humans or animals. -
Page 6: Technical Specifications
GENETIX > CLONEPIX FL ROBOT MANUAL Technical Specifications External Dimensions and Weight Size (excluding ancillary equipment): 1010mm (width) x 900mm (depth) x 1495mm (height) Weight: Robot - 350 kg (maximum) Compressor - 60 kg Compressed Air Supply Minimum Pressure: 6 bar... -
Page 7: Electrical Supply
GENETIX > CLONEPIX FL ROBOT MANUAL Electrical Supply European: Voltage: 210 - 250V AC 45-66 Hz single phase Power: Robot - 2500W Halogen Heater - 300W Connections: IEC Input - Robot Mains / Halogen Heater IEC Output - PC IEC Output - Monitor Fuses: Input - F3 –... -
Page 8: Electrical Connections
GENETIX > CLONEPIX FL ROBOT MANUAL Electrical Connections Figure 1: ClonePix FL electrical connections Symbols on Equipment Symbol Meaning Beware moving parts. Refer to Robot Manual for operating instructions. The year of manufacture is given on the serial number label which is located on the right side of the unit. -
Page 9: Pc Connections
GENETIX > CLONEPIX FL ROBOT MANUAL PC Connections Figure 2: ClonePix FL PC connections 9 of 37... -
Page 10: Filter Options
GENETIX > CLONEPIX FL ROBOT MANUAL Filter options Filter set Excitation Emission Cat. No. Suitable for: Wavelength (nm) Filter Wavelength (nm) Filter Position Position X4990 AF430 (Royal Blue) (Cyan) X4991 CloneDetect FITC* (standard) (Blue) (Green) Fluorescein AF488 X4992 (Cyan) (Yellow) -
Page 11: Robot Enclosure
ROBOT MANUAL Robot Enclosure ClonePix FL is designed to provide a controlled environment for sample protection conducive to accurate and reliable mammalian cell picking. Protection within the robot top cover is provided by utilizing a rear mounted HEPA filtered air supply and a front mounted exhaust fan. This creates a horizontal laminar flow of sterile air across the picking area of the bed of the machine whilst the front door is closed. -
Page 12: Installation
GENETIX > CLONEPIX FL ROBOT MANUAL Installation Note: Installation is to be undertaken only by Genetix approved personnel. Layout of ClonePix FL and Ancillary Equipment Front view: Top view: Figure 3: ClonePix FL layout and dimensions 12 of 37... -
Page 13: Source And Destination Plate Stackers
ROBOT MANUAL Source and Destination Plate Stackers ClonePix FL uses a stacker system to hold and process the source and destination plates. The source stacker is located at the rear of the machine and the destination stacker towards the front. The stacker cassettes can accommodate up to 10 standard microplates or 12 Genetix microplates. -
Page 14: Connecting The Wash Bottles
The tubing carrying system fluid (sterile water) to the Wash Station and back to the waste bottles will be connected by a Genetix approved engineer on commissioning of ClonePix FL. If it is necessary to disconnect these make sure that they are marked so that the tubes can be re- connected correctly. -
Page 15: Camera Alignment
The aim of camera alignment is to achieve pin-to-spot accuracy, relating the image pixel co- ordinates with the robot x, y co-ordinates. Calibration and alignment are performed before ClonePix FL is dispatched and will be checked by a Genetix approved engineer when the robot is installed. -
Page 16: Health And Safety
External Covers Warning – If any of the external covers on ClonePix FL are removed the power supply is automatically interrupted. If it is necessary to remove any of the external covers it is essential that switched off the power is first. -
Page 17: Safety Features
Isolator Switch: Turns on power to ClonePix FL. The isolator switch is the round red switch located at the bottom of the right-hand side panel. ClonePix FL is powered up by turning the switch 90º from ‘O’ to ‘I’. Door: The machine will not run with the front door open. -
Page 18: Electrical Safety
Power is removed from the drives when the doors are open. Hot Air/Halogen Dryer ClonePix FL is fitted with an ultra-high temperature halogen dryer. The casing may become hot during the drying cycle. Noise Levels During normal operation the level of airborne noise emitted by the robot will not exceed 70db measured at a distance of 1 meter. -
Page 19: Initial Operation
ROBOT MANUAL Initial Operation System Features ClonePix FL is constructed within a welded steel framework. The outer measurements are given in the Technical Specifications section on page 6. Be aware that these dimensions do not allow for opening robot doors. -
Page 20: Power-Up Procedures
Ensure RESET button is pressed (red light goes off). • Once the computer has booted up, click on the ClonePix FL icon. The first time ClonePix FL is used, the three axes will sequentially go through their ‘Initialize drives’ routine. This enables the drives to find their respective datum positions. -
Page 21: General Maintenance
Weekly • In any week that ClonePix FL is not used, it is important to purge the system fluid supply line with sterile water, and run 70% ethanol through the replenishing ethanol wash bath (if fitted). Failure to keep the pumps regularly primed may lead to system malfunction. -
Page 22: General Precautions
Cleaning Procedures Removable Parts The design of ClonePix FL permits all components coming into close proximity with biological material to be removed and cleaned: Picking Head The picking head can be regularly sprayed with 70% ethanol in situ and allowed to dry in the HEPA laminar air flow. - Page 23 Autoclave pins, O-rings and pin removal key. Recommended standard autoclave conditions are 121° C at 15 psi (103 kPa) for 20 minutes. Genetix recommends that each project/group should have at least two sets of Picking Pins. Picking Tubing Picking Tubing (X4970) is the 8 silicon tubes that connect between the picking head and the actuator.
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Page 24: Cleaning Of Instrument Interior
Cleaning of Instrument Interior For efficient decontamination of pathogenic micro-organisms, all non-removable parts within the ClonePix FL top cover should be sprayed or wiped with Genetix Sterilizing Agent (K8080) or 70% Ethanol. Abrasive cleaners should not be used, as they will damage the surface of the bed. -
Page 25: Automated Picking Pin Cleaning
Described below are two typical sterility tests conducted on ClonePix FL. 1. Chamber sterility test A ClonePix FL was prepared using the cleaning procedures described above. A Trypticase Soy Agar* plate (BD Biosciences #221185) was placed open on the robot bed during a prolonged mammalian cell picking run. -
Page 26: Changing The Picking Head
Important note: When handling the picking head take care that the pins are not knocked. Damage to pins may produce unreliable results and can cause serious damage to ClonePix FL. If any pins are suspected to have incurred damage please replace them immediately. -
Page 27: Replacement Parts And Optional Extras
GENETIX > CLONEPIX FL ROBOT MANUAL Replacement Parts and Optional Extras Please refer to the Genetix website for the latest replacement parts and optional extras www.genetix.com Replacement Parts Code Description X4941 Additional picking head populated with eight X4961 (F1 Pins) -
Page 28: Reagents And Supplies
GENETIX > CLONEPIX FL ROBOT MANUAL Reagents and Supplies Please refer to the Genetix website for the latest reagents and supplies www.genetix.com CloneDetect Detection Reagents Cat No. Description Pack Size K8200 CloneDetect anti-human detection agent. FITC label. Sterile, azide free. -
Page 29: Clonematrix
GENETIX > CLONEPIX FL ROBOT MANUAL CloneMatrix Cat No. Description Pack Size K8510 CloneMatrix 1 x 40ml (100ml final media volume) K8500 CloneMatrix 6 x 40ml (6 x 100ml final media volume) K8530 CloneMatrix: optimized for CHO cells 1 x 40ml (100ml final media volume) -
Page 30: Petriwell Cell Culture Plates
GENETIX > CLONEPIX FL ROBOT MANUAL PetriWell Cell Culture Plates Cat No. Description Pack Size W1000 PetriWell-1 Plate. Black with clear base. Polystyrene. TC treated 1/40 W1005 PetriWell-1 Plate. Black with clear base. Polystyrene. Non TC treated 1/40 W1007 PetriWell-1 Plate. Black with clear base. Polystyrene. Collagen coated... -
Page 31: Other
Clean pin cleaning solution K8080 Genetix Sterilizing Agent (1L per Sachet) K8085 Genetix Sterilizing Agent (1L per Sachet) K8150 CaliBeads: Fluorescent beads for ClonePix FL. 200µm diameter. Pan-wavelength. 100ml K8010 Adherent Cell Picking Reagent – Type A 1 x 500ml K8020 Adherent Cell Picking Reagent –... -
Page 32: Troubleshooting
Manually move the actuator to the middle of the bed Disconnect and reconnect control cable between PC and right side of ClonePix FL (a small screwdriver may be necessary). Re-boot ClonePix FL Interior light or UV germicidal lamp will not turn... -
Page 33: Glossary Of Terms
Default location for Pick Run and Imaging Run images and data. Accessible via Review Results. LED intensity ClonePix FL uses only LEDs for consistent and reliable imaging. The intensity of the LEDs can be adjusted by the user to control image exposure under the Acquisition tab. - Page 34 (trans). The Prime Probe is set up by creating a Prime Configuration. Process A standard program for ClonePix FL to carry out a task such as a series of similar experiments, or a maintenance task. A Process is saved as a .gfp file.
- Page 35 GENETIX > CLONEPIX FL ROBOT MANUAL Wash bottle 4 liter bottle of sterile water used to purge the fluid system (1 liter in some older systems). Waste bottle 4 liter bottle used to collect purged fluid (1 liter in some older systems).
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Page 36: Index
GENETIX > CLONEPIX FL ROBOT MANUAL Index Accuracy Maintenance Actuator 15, 19 Maximum Air supply Mechanical interlock Airborne noise Microplates Ball-screw Operating environment Bath 13, 19 Pin(s) 13, 15, 19 Power outlet Calibration Power-Up Procedures Calibration and alignment Pre-Power-Up Checklist... -
Page 37: Contact Details
Information furnished by Genetix Ltd is believed to be accurate and reliable; however, no responsibility is assumed by Genetix Ltd, for its use; nor for any infringements of patents or other rights of third parties which may result from its use. No license is granted by implication or otherwise under any patent rights of Genetix Ltd.
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