Related Manuals for Genetix ClonePix FL
Summary of Contents for Genetix ClonePix FL
- Page 1 ClonePix Quick Set-Up Instructions Software Release: 1.2.13.971 07MAN1055.A2 Effective Date: 01-Jun-10...
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Page 2: Table Of Contents
GENETIX > CLONEPIX FL QUICK INSTRUCTIONS > CONTENTS Contents What are Quick Set Up Instructions?...............3 Starting up ClonePix FL ..................3 Loading plates....................4 Preparing for a Pick Run ...................5 The Pick Run ......................6 Imaging Settings........................7 Picking Settings ........................8 Sanitize Pin Options ......................9 Start Pick Run........................ -
Page 3: What Are Quick Set Up Instructions
Ensure compressed air gauge is set to 80 psi (5.5 bar). • Ensure Emergency Stop button is not pressed in. • Switch on ClonePix FL. The HEPA filtration system works all the time that ClonePix FL is on. • Press Reset button at the front of ClonePix FL. •... -
Page 4: Loading Plates
QUICK INSTRUCTIONS > LOADING PLATES Loading plates It is important to know how to load plates into ClonePix FL correctly. A ClonePix FL bed layout is as shown below. The Source plate stacker system is located at the rear of the machine, and the Destination plate stacker system is at the front. -
Page 5: Preparing For A Pick Run
Orientation of destination plates in ClonePix FL Preparing for a Pick Run Prior to carrying out a picking run, it is important to make sure that ClonePix FL is set up correctly. The Prepare for Pick Run process is designed for this purpose. This process helps the user to validate that 1) the pins are firing correctly, 2) the camera, pins and microplates are aligned, and 3) the fluid system is sterile and ready for use. -
Page 6: The Pick Run
GENETIX > CLONEPIX FL QUICK INSTRUCTIONS > THE PICK RUN The Pick Run The Pick Run process is designed to aid new users through their first picking run. Use of the default parameters provided should facilitate successful imaging, colony selection and picking. -
Page 7: Imaging Settings
• Select Read Barcode. Select Auto-assign barcode in case of failure. If you don’t have barcodes on your plates, ClonePix FL will automatically assign a code. Batch plates • Select this option. This assumes that you are loading multiple plates that contain the same sample and that you want them to be processed as a single experiment. -
Page 8: Picking Settings
GENETIX > CLONEPIX FL QUICK INSTRUCTIONS > THE PICK RUN be created later in Preview (see below). Most users select one white light and one fluorescent option. Prime Probe • The Prime Probe is the acquisition option to be used for colony detection. Select the white light option if available. -
Page 9: Sanitize Pin Options
• Select Read Barcode. Select Auto-assign barcode in case of failure. If you don’t have barcodes on your plates, ClonePix FL will automatically assign a code. Deposit Options • Select Match Destination Plate to Source Plate if you want to start a new destination plate each time a new source plate is fed in. -
Page 10: Preview
GENETIX > CLONEPIX FL QUICK INSTRUCTIONS > THE PICK RUN Preview This is where you set up your Image Acquisition and Colony Detection settings. The screen should look like this: Step 1: Set up Image Acquisition settings Click on the Acquisition tab. - Page 11 White Light and FITC 1s. Insure that White Light and FITC 1s options are selected and that Default is deselected. Do not delete the Default option – it is required for other ClonePix FL functions. Set the Prime Configuration to White Light. This is critical for correct colony detection.
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Page 12: Select Wells
Click Next to proceed. • Imaging ClonePix FL will now automatically image the source plates, detect the colonies and generate combined data for all plates. While ClonePix FL is imaging, pre-fill one or more 96-well destination plates with liquid •... -
Page 13: Select Groups
GENETIX > CLONEPIX FL QUICK INSTRUCTIONS > THE PICK RUN To further isolate only those in the Accept group that • have highest associated FITC fluorescence, click New Group. This will bring up a new group at the top. Click on the newly-created group (top line) to select it •... -
Page 14: Picking
Before picking can commence, you will be prompted to swap the source cassettes. Remove • the empty Feed cassette (it can be placed at the back of the ClonePix FL bed). Next, unlock the Return cassette and carefully transfer it to the left side of the source stacker. There is no need to re-arrange the plates in the cassette. -
Page 15: Contact Details
Information furnished by Genetix Ltd is believed to be accurate and reliable; however, no responsibility is assumed by Genetix Ltd, for its use; nor for any infringements of patents or other rights of third parties which may result from its use. No license is granted by implication or otherwise under any patent rights of Genetix Ltd.
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