Dagan 2400A Operating Manual

Dagan 2400A Operating Manual

Extracellular preamplifier

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Extracellular Preamplifier
Operating Manual
Warning: This instrument is not designed or intended for use in
human applications or human experimentation
2400A
深圳市净康科技有限公司
地址:深圳市龙岗区南湾街道吉厦社区沙平北路 111 号 6008
王经理 15813841944
(微信同号)
电话:0755-28917660
邮箱:jkang66@163.com
网址:http://www.3000buy.com
QQ : 422612157

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Summary of Contents for Dagan 2400A

  • Page 1 2400A Extracellular Preamplifier Operating Manual 深圳市净康科技有限公司 地址:深圳市龙岗区南湾街道吉厦社区沙平北路 111 号 6008 王经理 15813841944 QQ : 422612157 (微信同号) 电话:0755-28917660 邮箱:jkang66@163.com 网址:http://www.3000buy.com Warning: This instrument is not designed or intended for use in human applications or human experimentation...
  • Page 2: Table Of Contents

    Use of High and Low Cutoff Filters b) Monitor Amplifier 5. Probe a) Capacitive Neutralization Control b) Probe Input Parameters c) Probe Input Connector d) System Grounding e) Physical Probe Placement C. MODEL B (external DC/Battery operation) D. SERVICING E. GUARANTEE DAGAN CORPORATION Minneapolis, Minnesota USA...
  • Page 6: General Information

    When used by itself or as a component of a larger system, this instrument will truly become an indispensable laboratory tool. INSTRUMENT & ACCESSORIES The Model 2400A is shipped with the following standard accessories: Rack adapter (handles/bracket assemblies). Probe mounting rod for micromanipulator...
  • Page 7: Specifications

    SPECIFICATIONS PREAMPLIFIER NEUTRALIZATION: 15 turn control located on probe. Range is 0 to 100 INPUT IMPEDANCE: Greater than 10,000 Megohms INPUT CONNECTOR: Miniature co-axial slip-on OUTPUT: 10 ohms impedance. 20 volt peak to peak, BNC connector GAIN: 50; 100; 200; 500; 1,000; 2,000; 5,000; or 10,000 ± 1% OVERLOAD RECOVERY: Recovery is very fast, usually one-half to a few milliseconds except for just after STAIN.
  • Page 8: Options/ Input Connectors

    2400 OPTIONAL HEADSTAGES 2410A Standard Capacitive Neutralized Single ended probe and cable with connector. 2430A Optional Differential probe for the 2400A System Input Adapters and Connectors 8012 Additional co-axial input connector HB120 and HB180 Glass Microelectrode Holders for BNC option input...
  • Page 9: Directions For Use

    DIRECTIONS FOR USE INSTALLATION 1. LINE VOLTAGES AND FREQUENCY The serial number label on the instrument rear panel also contains the line voltage and frequency. A voltage marked 115v AC indicates the power supply is wired for voltages from 100 to 125v AC. A 230v AC marking indicates a 200 to 250v AC range.
  • Page 10: Operation

    OPERATION ELECTRODE The ELECTRODE control switches provide a unique combination of microelectrode terminations~ A push of the button changes the 2400 system from (1) a high current stain generator for marking recording sites to a, (2) controlled positive or negative ideal current source up to 1000nA to (3) a low noise neutralized preamplifier to your microelectrode for any future need that you may encounter.
  • Page 11: Current Pump

    INTERNAL position of the PUMP Level switch) and the microelectrode resistance should be less than 14 volts. For situations requiring higher compliances than 14 volts the DAGAN model 6400 Current Generator system (40 volts compliance) is recommended. b) EXTERNAL PUMP REFERENCE Using a BNC connector, inject a DC analog voltage within -10 to +10 volts into the EXT REF jack.
  • Page 12: C) Auxiliary Pump Output

    PUMP External Reference input is one meghom. An output current of 1nA for each 10mV of reference voltage will be available either through the probe or the AUX PUMP OUT BNC connector. c) AUXILIARY PUMP OUTPUT (AUX PUMP OUT) USE The output of the current generator may be obtained at any time from this connector.
  • Page 13 In instruments equipped for microelectrode resistance measurements (2400Z), the CALIBRATE generator will be modified to a 2 Hz output. The model 2400 is normally supplied with a 150 Hz CALIBRATE generator.
  • Page 14: Preamplifier

    PREAMPLIFIER The heart of the 2400 system is its versatile preamplifier section made up of a high gain, low noise neutralized preamplifier, active filters, and an audio amplifier. The neutralized input capacitance allows risetime signals to be viewed with high microelectrode resistances.
  • Page 15: A) Use Of High And Low Cutoff Filters

    an option) plugs into the instrument back panel to provide sound where you want it. A Calibrate switch allows a precise signal to be passed from the preamplifier to calibrate recording or viewing instrumentation. a) USE OF HIGH AND LOW CUTOFF FILTERS The active filter network provided in the 2400 is virtually a complete instrument in itself.
  • Page 16: B) Monitor Amplifier

    A rear panel mounted BNC PULSE TEST 10:1 allows you to inject a calibration signal through the 2400A (after the GAIN) to check external recording equipment. For example, a one volt signal would...
  • Page 17: Probe

    PROBE An ultra low noise experimental setup and the absence of capacitive loading effects are made possible by the use of a probe head stage containing the first preamplifier gain block and also all circuitry needed for the other microelectrode terminations. Capacitive neutralization is at your fingertips with a high resolution neutralization control located on the probe.
  • Page 18: B) Probe Input Parameters

    negative neutralization capacitance fed back to the probe input) will result in a much faster risetime. The neutralization control is preadjusted at the factory and in many cases will never need additional attention. For very high microelectrode resistances (above 10 megohms) or experimental setups with a large amount of stray (or a co-axial cable) capacitance, slight adjustments from time to time will be desirable.
  • Page 19: C) Probe Input Connector

    DC coupled input stage allows extremely fast preamplifier recovery if overloads or open circuits occur. c) PROBE INPUT CONNECTORS The 2400A comes with BNC connector, Mini Coaxial, 1mm Tip Jacks, or Phone Jack BNC (standard in 2005) Mates with Dagan HB120 and HB180 microelectrode holders for glass pipettes.
  • Page 20 differential FET amplifier connected to ground). To achieve maximum advantage of the low noise preamplifier input, the probe should be located as close as possible to the microelectrode. The usual procedure is to use a 4 to 6 inch piece of small diameter co-axial cable to connect the microelectrode internal to the 2400 probe input connector.
  • Page 21: Model B (External Dc/Battery Operation)

    DC supplies or batteries. D. SERVICING Dagan Corporation prefers that we be allowed to perform any major service work that should arise. The three year warranty period will provide service at no charge to your during this period.
  • Page 22: Guarantee

    The 2400 system is warranted to be free from defects caused by materials, workmanship, and construction for a period of one year from date of purchase. During this period, Dagan Corporation will service your instrument at no charge. A letter or phone call to us can usually provide enough data to confirm the problem and we will then immediately air ship you the plug-in replacement cards.

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