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Dagan IX2-700 Operating Manual

Dual intracellular preamplifier
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IX2-700
Dual
Intracellular Preamplifier
Operating Manual
Version 1.1
Dagan
Corporation
2855 Park Avenue
Minneapolis, MN 55407 USA
(612) 827-5959
Fax: (612) 827-6535
Email: info@dagan.com
Web: www.DAGAN.com
Warning: This instrument is not designed or intended for use in human applications or human experimentation.

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Summary of Contents for Dagan IX2-700

  • Page 1 IX2-700 Dual Intracellular Preamplifier Operating Manual Version 1.1 Dagan Corporation 2855 Park Avenue Minneapolis, MN 55407 USA (612) 827-5959 Fax: (612) 827-6535 Email: info@dagan.com Web: www.DAGAN.com Warning: This instrument is not designed or intended for use in human applications or human experimentation.

  • Page 2: Table Of Contents

    IX2-700 Dual Intracellular Preamplifier TABLE OF CONTENTS Page IX2-700 Block Diagram Introduction and Features Control Description Operation Specifications Accessories Warranty/Services/Repairs...

  • Page 4: Introduction And Features

    Two Channel extracellular recording; Ion-Selective Electrode Amplifier; Teaching application requiring ease of use. The IX2-700 is a complete instrument with DC and pulsed current passing capability, filtering, electrode impedance testing, and a back lighted (for dark labs) LCD voltage and current meter.

  • Page 5: Control Description

    BUZZ OPERATION The buzz or tickle function contained in the IX2-700 is used to impale an electrode in the cell. The IX2-700 has a controlled buzz magnitude. Start with the Buzz Magnitude in the counterclockwise or minimum position and increase while initiating buzzes until you achieve desired cell penetration.
  • Page 6 IX2-700 Dual Intracellular Preamplifier OFFSET VOLTAGE CONTROL This control cancels electrochemical potentials developed between reference electrodes and the probe input. Offsetting the output level of the amplifier nulls these potentials. The range is ±200 mV. Be careful not to touch this control during the experiment, as it will change the baseline.
  • Page 7 IX2-700 Dual Intracellular Preamplifier EXTERNAL I BNC The External I BNC allows for externally generated step commands at 20nA/V times the headstage multiplier. 1/3/5/10/30 KHZ WB FILTER SWITCH The low pass filter limits the high frequency response of the amplifier to minimize noise.

  • Page 8: Operation

    IX2-700 Dual Intracellular Preamplifier OPERATION Each of the two independent channels in the IX2-700 can be used as a bridge Channels 1 and 2 have Bridge Balance and Holding Current controls. PROCEDURE When beginning, ascertain that the system is grounded properly and that the noise is low.
  • Page 9 IX2-700 Dual Intracellular Preamplifier Apply a train of current pulses to the electrode. Note that the Z test may not be used for this as it disables the Bridge Balance control. This can either be from an external source or from the Step Current Command. You will see a train of pulses on the voltage trace of the oscilloscope.
  • Page 10 IX2-700 Dual Intracellular Preamplifier COMPENSATING FOR THE CAPACITY The capacity compensation control provides capacitance neutralization, or “negative capacitance” at the headstage input. Without this compensation, high frequency signal components from a high impedance source such as a microelectrode would be lost because of stray capacitances at the input. Apply a square wave (such as in balancing the bridge) to the electrode.
  • Page 11 IX2-700 Dual Intracellular Preamplifier DIFFERENTIAL RECORDING The IX2-700 can be used for differential recording using Channel 1 and Channel 2. The 10 (V ) output BNC on the front panel is the Channel 1 voltage less the Channel 2 voltage. You must be certain both channels are properly zeroed with their DC offsets as any offset will also be subtracted from the other channel.

  • Page 12: Specifications

    IX2-700 Dual Intracellular Preamplifier SPECIFICATIONS Electrode Holders: Low noise polycarbonate in two sizes: 1.0 to 1.2mm (#HBI2O); 1.5 to 1.8 mm (#HB18O) (order separately) Headstage: Box Probe, 1.5” x 1.2” x .88”, 4” long, 3/8” rod, BNC Input Connector Standard Headstage: N=.l for 20 to 500 Megohm...

  • Page 13: Accessories

    IX2-700 Dual Intracellular Preamplifier Balance Reading: 0 to 100 megohm divided by headstage scaling N (ten-turn dial) Electrode Impedance Test: 1mV per megohm squarewave (N) Digital Polarization Current Range: 0 to +100 nA (ten-turn dial) scaled by headstage multiplier N...

  • Page 14: Warranty/Services/Repairs

    FUSE REPLACEMENT The IX2-700 has a fused power supply. The fuse is located inside the cabinet on the rear panel printed circuit board. It is easily snapped out for inspection if the power switch pilot light should fail to function. It is a slow- blow 1 amp.