Page 1 Operating manual HettCube 200/200 R; 400/400 R; 600/600 R Translation of the original operating manual Read the instructions prior to performing any task! AB66006 12.2020 / Rev. 01 1 / 173...
Page 2 Andreas Hettich GmbH & Co. KG Föhrenstraße 12 D-78532 Tuttlingen/Germany +49 (0)7461/705-0 +49 (0)7461/705-1125 info@hettichlab.com, service@hettichlab.com www.hettichlab.com © 2019 2 / 173 12.2020 / Rev. 01 AB66006...
Page 3: Table Of Contents
Table of contents Table of contents Introduction ........7 Symbols.
Page 4 Table of contents Switching on the incubator....46 Initialization....... . 46 Operating modes.
Page 5 Technical data ........133 HettCube 200......133 HettCube 200 R.
Page 6 Table of contents External devices are integrated in program mode, e.g. DAY/NIGHT simulation..... 163 Combination of standstill mode with incubation mode (energy-saving mode).
Page 7: Introduction
Introduction Introduction Symbols Signal words Signal word Meaning DANGER This combination of symbol and signal word indicates an immediate dan- gerous situation that will result in death or serious injury if it is not avoided. WARNING This combination of symbol and signal word indicates a possible dangerous situation that can result in death or serious injury if it is not avoided.
Page 8 98/79/EC. Please observe the intended Chapter 1.5 ‘Intended use’ on page 12 . use in this regard  For IvD applications, Hettich offers IvD-compliant incubators. Fig. 2: Information on the front side of the incubator 8 / 173 12.2020 / Rev.
Page 9 Introduction Attention, general danger point Nonobservance of this warning can lead to material damage and personal injury. Before using the incubator, make sure you read the operating instructions and observe the safety information. Attention: Biohazard Nonobservance of this warning can lead to personal injury. Before using the incubator, make sure you read the operating instructions and observe the safety information.
Page 10: Symbol On The Shipping Carton Label
Introduction Symbol for potential-free alarm output This symbol indicates the potential-free alarm output. Symbol for fuse (for Switchboard option only) This symbol indicates the fuse 6F1 (for Switchboard option only). Symbol for analogue output for independent temperature measure- ment (option) This symbol indicates the 4-20 mA analogue output for an inde- pendent temperature measurement.
Page 11: Personal Protective Equipment
Andreas Hettich GmbH & Co. KG. Service technician A service technician is someone who was trained and authorized by Andreas Hettich GmbH & Co. KG to perform service work on the incubator. AB66006 12.2020 / Rev. 01...
Page 12: Intended Use
Improper use Another use or a use going beyond the intended use is considered to be improper. The company Andreas Hettich GmbH & Co. KG is not liable for damage resulting from this. ■ The cultivation of human sample material for diagnostic purposes,...
Page 13: Replacement Parts/Consumable Material
■ Due to the legal regulations and especially to protect the Hettich sales partner and Hettich employees, clean and disinfect the incu- bator and accessories and certify that it is harmless with your sig- nature (clearance certificate).
Page 14: Safety Information
Safety information Safety information ■ Before commissioning the incubator, the operating instructions are to be read and observed. Only those persons who have read and understood the operating instructions may operate the incu- bator. ■ In addition to the operating instructions and the binding regula- tions for accident prevention, the recognized professional rules for safe and proper work are to be observed.
Page 15 Repairs may only be performed by a person authorized by the manufacturer. ■ Only original spare parts and approved original accessories of the company Andreas Hettich GmbH & Co. KG may be used. ■ The safety and reliability of the incubator are only guaranteed if: –...
Page 16: Device Description
Device description Device description Standard device Touchscreen incl. ■ Temperature guard, class 3.1; for cooled devices, class 3.1 and Chapter 6.13.5 ‘Temperature 3.2.; individually adjustable  guard’ on page 98 . ■ Chapter 6.13.4 ‘Tol- Tolerance band asymmetrically adjustable  erance band’...
Page 17 Device description The incubator has a access port with a Ø of 42 mm on the rear side. Through this opening, cables from external measuring systems can be fed into the interior. After feeding through the cable, the access port must be sealed with the foam plug included in delivery to prevent temperature deviations in the inte- rior.
Page 18: Options
Device description Fig. 8: Rear side of device (size 200) For device size 200 only: Thread insert M6 for fixing kit 60012 Mains cable connection Potential-free analogue output Standard access port, Ø 42 mm Options Options are articles which are permanently installed in the device.
Page 19: Additional Device Access Port
Device description Fig. 9: Condensation diagram 3.2.2 Additional device access port Additional device access port (Ø 22, 42, 67 mm) centred on the left side of the device. Other positions possible after consultation. In addition to the standard access port, it is possible to realize addi- tional access ports.
Page 20: Independent Pt100 Temperature Sensor With 4-20 Ma Analogue Output
3.2.5 Continuous cooling (option) For applications in continuous operation for which temperatures below 15 °C are required, Hettich offers an alternative operating mode in its cooled incubators. After the long-term cooling phase has ended, perform the subse- quent dehumidification process. Particularly when the device is tem- porarily put out of operation after long-term cooling.
Page 21: Passive Dehumidification
Device description Icing can occur in the case of continuous cooling temperatures between +1°C and +5°C. Using pro- gram mode, the customer can create a suitable "defrosting" program. The device runs through a daily cycle in which the cooling unit is briefly heated, and then specifically cooled again afterwards.
Page 22 Device description Drawer (HTS set), 30 60024 mm high Drawer (HTS set), 65 60025 mm high Drawer (HTS set), 105 60026 mm high Rack 60040 Rack (set) 60039 Rack (HTS set) 60038 Rack 60041 Rack (set) 60037 Rack (HTS set) 60036 Frame L, 16 places 60027...
Page 23 Device description USB port blocker (set) - 60525 For protecting the USB/A interface. Set consists of 10 locking clips and 1 USB key tool. AB66006 12.2020 / Rev. 01 23 / 173...
Page 24: Transport And Storage
The length and width of the pallet are identical for all incubators. The incubator height can be found in the following table. Incubator Height HettCube 200/200 R 1111 HettCube 400/400 R 1565 HettCube 600/600 R 2132 Tab. 1: Height with packaging...
Page 25 Transport and storage HettCube HettCube HettCube 200/200 R 400/400 R 600/600 R Drawer (HTS set), height 30 mm (kg) Drawer (HTS set), height 65 mm (kg) Drawer (HTS set), height 105 mm (kg) Rack (set) for inclined storage of cultures (Loewenstein) (kg) Rack (HTS set) for inclined storage of cul- tures (Loewenstein) (kg)
Page 26 Transport and storage DANGER A danger is posed by penetrating liquids. There is an electric shock hazard for personnel. − If liquid has penetrated into the incubator, the incubator must not be put into operation. − In such a case, contact a service technician of the manufacturer.
Page 27: Commissioning
Commissioning Commissioning ■ Ventilation openings may not be covered or blocked. ■ Before commissioning, check to make sure the mains voltage is correct according to the nameplate and that the mains cable is connected properly. ■ If necessary, additional devices and accessories can be mounted and/or connected.
Page 28 Commissioning Remove the two fastening screws and remove the front wooden beam. In the case of devices with the optional glass door, there are additional reinforcements attached to the wooden beam. These can be removed with the beam. Fig. 15: Front wooden beam Fastening screws Front wooden beam Fasten the two metal rails to the wooden pallet with two nails...
Page 29 Commissioning Roll the incubator carefully down from the wooden pallet using the metal rails. Fig. 19: Rolling the incubator down over metal rails Unloading from the pallet, HettCube 200/200 R Remove the front wooden beam. Fig. 20: Front beam Fastening screws Front beam Stick the washers included in delivery onto the metal bars and screw the metal bars into the two holes on the rear panel.
Page 30: Setting Up, Connecting And Switching On The Incubator
Commissioning Hold the two metal bars and carefully pull the incubator off the wooden pallet using the metal rails. NOTICE Do not lift the incubator with the metal bars, as otherwise the rear panel may be damaged. Unscrew the metal bars from the rear panel of the incubator. Successively lift the incubator on the left and right sides slightly and remove the two metal rails.
Page 31: Incubator Connection
CAUTION Two incubators may only be stacked on top of each other in the case of HettCube 200/200 R. The upper incubator must be fixed to the lower incubator with the stack kit (order no. 60009) and additionally secured against tilting. We rec- ommend the fixing kit (order no.
Page 32 Commissioning DANGER A danger is posed by penetrating liquids. There is an electric shock hazard for personnel. − If liquid has penetrated into the incubator, the incubator must not be put into operation. − The incubator may only be operated at a socket with a personal protection switch.
Page 33 Commissioning Potential-free alarm output The connection may only be established by qualified, skilled per- sonnel. NOTICE Material damage to the controller board due to high load. Relays on the controller board can be damaged in the event of a high load, and will no longer switch. −...
Page 34 Commissioning Connection of additional devices NOTICE (Switchboard option) An excessively high interior temperature can result in damage to the incubator. External devices which are operated inside the incu- bator must not exceed the permitted power, since the uncontrolled temperature increase can result in damage to the incubator.
Page 35: Initial Commissioning
Commissioning 5.2.3 Initial commissioning Switch on the incubator at the device switch. The incubator can be operated via the touchscreen. The touchscreen can also be operated with latex gloves. Fig. 28: Device switch [ON/OFF] Device switch  The control will start up. Fig.
Page 36 Commissioning [Confirmation] button to confirm the language selec- Tap the tion. Fig. 32: ‘Language’ selection [Confirmation] button ‘Selected language’ display Date and time are already preset. If the preset infor- mation is correct, confirm this with the [Confirmation] button. The start screen (Fig. 38 ‘Start screen’ on page 37) is displayed and the configuration is completed.
Page 37 Commissioning [Confirmation] button Keypad Tap the date. Fig. 35: Setting the date and time Date [Confirma- Select the current date. Confirm the entry with the tion] button. Fig. 36: Setting the date [Confirmation] button Date selection [Cancel] button [Confirma- Confirm the entries for the date and time with the tion] button.
Page 38: Inserting And Removing The Slide-In Modules
Commissioning These settings are only made once. When switched on again, the start screen appears. The description for operation can be found in Chapter 6 ‘Operation’ on page 41 .  Inserting and removing the slide-in modules In the delivered condition, the slide-in modules and drawers are already inserted in the incubator.
Page 39: Telescopic Slide-In Modules
Commissioning 5.3.2 Telescopic slide-in modules Before inserting the slide-in modules and drawers, the telescope rails included in the delivery must be inserted. Inserting the telescope rails Push the telescope rails into the rear latching rail at the desired height. Fig. 42: Snapping telescopic rails in at the rear Engage the telescope rails in the front latching rail.
Page 40 Commissioning Removing the telescopic rails Lift the slide-in module or the drawer, unhook it from the tele- scope rails and remove it. Pull the telescope rails out a little. Hold the telescopic rails at the pulled-out part and slightly twist towards the centre of the incubator until the snap-in nose proj- ects from the latching rail.
Page 41: Operation
(60919). − When operating a vibrating device (e.g., a shaker), the operating company must make sure that this cannot move away on the slide-in module. Hettich accepts no responsibility for damage caused by the use of external devices. −...
Page 42 Operation WARNING There is a risk that the sample temperature control will be faulty. There is a risk that the temperature control of micro- biological samples is not correct, which could result in a misinterpretation of the test results or the destruc- tion of the samples.
Page 43: Operating Elements
Operation Before operation, complete commissioning in full  Chapter 5 ‘Commissioning’ on page 27. After a malfunction (power failure, door open too long, etc.), the operating company must assess whether the samples are still usable. Operating elements Fig. 48: Operating elements, front side Door lock Service interface (USB) [Incubator On/Off] switch...
Page 44: Door Locking Mechanism
Operation Door locking mechanism To prevent unauthorized personnel from opening the door, the incu- bator can be locked. Individual cylinder locks are used. If the key is lost, a spare key can be ordered as a spare part by providing the lock number.
Page 45: Fixing The Mechanical Display Protection Of The Control Panel
Operation Fixing the mechanical display protection of the control panel To prevent the incubator from being operated by unauthorized per- sonnel, the mechanical display protection can be additionally blocked. In the delivery condition, there is a screw on the rear side of the incubator, which can be used to block the mechanical display protec- tion.
Page 46: Switching On The Incubator
Operation Switching on the incubator Switch on the incubator at the device switch. NOTICE When switched on, the ring lighting of the device switch is active. Fig. 52: Device switch [ON/OFF] Device switch  The control will start up. The start screen is displayed. Fig.
Page 47: Operating Modes
Operation Operating modes The incubator offers the following operating modes: ■ Chapter 6.8.1 ‘Functional description of stand- Standstill mode  still mode’ on page 47 In this operating mode, only the current interior temperature is displayed. The interior temperature is not regulated. Individual set- tings on the temperature guard as well as timer-controlled starting times for manual or program operation are displayed.
Page 48: Functional Description Of Manual Mode
Operation 6.8.2 Functional description of manual mode In manual mode, an adjustable temperature is held constantly. With ‘Editing or deleting temperature drop’ temperature drops  on page 62 , you can raise or lower the temperature setpoint to a definable value at certain times. Manual mode can be started with a time delay.
Page 49: Functional Description Of Program Mode
Operation 6.8.3 Functional description of program mode Via program mode, you can combine complex sequences of temper- atures and durations to form one program. A program is composed of program segments. A program segment can also be defined as a standstill segment (incubator in standstill without regulation).
Page 50: Main Screen
Operation After the execution of program mode, the incubator either changes to standstill or regulates to the holding temperature when this was defined at the program start. Main screen Operating concept Category 1 Light background (light grey) The incubator is either in standstill mode in manual mode or program mode.
Page 51 Operation Fig. 56: Main screen Item Designation Description/function Chapter 6.11 Call of manual and program mode. Operating modes  ‘Manual mode’ on page 54 ,  Chapter 6.12 ‘Program mode’ on page 69 ‘Date’ and ‘Time’ display Shows the current date and time. These can be changed in the settings (3).
Page 52: Process Flow Information
Operation The active temperature guard is indicated on the main screen. Fig. 57: Main screen, standstill mode, temperature guard active Temperature guard active display 6.10 Process flow information In the process flow information, you can enlarge the process flow in three different levels.
Page 53 Operation Level (type number) Scale Displayed time period Level 2 (type 2x) One hour 24 hours are displayed. One can scroll over seven days. Level 3 (type 3x) Five minutes 60 seconds are displayed. One can scroll over twelve hours. The display can be scrolled horizontally to the left and right.
Page 54: Manual Mode
Operation 6.11 Manual mode In manual mode, a specified temperature (temperature setpoint) is held. On the main screen, the rotating circle symbol, together with the symbol for manual mode, are displayed to indicate that temperature control is active. In the case of cooled incubators, temperature drops can be activated in manual mode.
Page 55 Operation Fig. 63: Main screen, temperature drop exists and active Temperature drop exists and is cur- rently active (black symbol) Fig. 64: ‘Manual mode  Settings’ AB66006 12.2020 / Rev. 01 55 / 173...
Page 56 Operation Item Designation Description/function Here, you can make settings for manual mode. ‘Manual mode’ tab  Chapter 6.11 ‘Manual mode’ on page 54 Here, you can define the date and time for the starting ‘Start delay’ Start delay  point of manual mode. If the start delay is deactivated, on page 57 manual mode is started without a time delay, directly after activating manual mode.
Page 57 Operation Start delay To set the start delay, proceed as follows: [Activate/deactivate start delay] Activate the start delay with the switch. Fig. 65: ‘Manual mode  Start delay’ [Activate/deactivate start delay] switch By tapping on the start date, you will get to the date selection. Fig.
Page 58 Operation By tapping on the start time, you will get to the time input. Fig. 68: ‘Manual mode  Start delay Start time’  Start time Enter the time. Confirm the input of the start time with the [Confirmation] button. Fig.
Page 59 Operation Temperature drop The temperature drop is only available in manual mode on incubators with a cooling unit. If the current manual mode contains a temperature drop, this is indicated in the HettCube activities  Further information on page 54. Multiple temperature drops can be created.
Page 60 Operation Creating a temperature drop To create the temperature drop, proceed as follows: Activate the temperature drop. Fig. 70: ‘Manual mode  Temperature drop’ [Activate/deactivate temperature drop] switch [Edit temperature drop] button. Tap on the Fig. 71: ‘Manual mode  Temperature drop ...
Page 61 Operation [Plus] button. Tap on the Fig. 73: ‘Manual mode  Temperature drop  Editing  Settings  Date’ [Plus] button Examples for the temperature drop can be found in the appendix  Appendix B ‘Examples’ on page 159. Fig. 74: ‘Manual mode  Temperature drop ...
Page 62 Operation Item Designation Description/function [Confirmation] button After you have made all settings, you can confirm this with this button. Starting time Enter the starting time (date and time). Duration You can define the temperature drop via a start and end time or via the start time and specifying the duration of the temperature drop here.
Page 63 Operation [Edit temperature drop] editing icon for the tempera- Tap on the ture drop that you would like to edit or delete. If you would like to delete all temperature drops, you can select this later. With the ‘List view’ symbol, you can have all temperature drops displayed in a list.
Page 64 Operation PIN protection If no ‘Admin PIN’ was assigned, you will still be asked for the ‘Admin PIN’ when activating the PIN protection. To activate the PIN protection, you will need the ‘Admin PIN’. To set the PIN protection, proceed as follows: [PIN protection] switch.
Page 65 Operation You can activate or deactivate the control contact for manual mode [Activate/deactivate control contact] switch. via the Fig. 82: ‘Manual mode  Control contact’ [Activate/deactivate control con- tact] switch Set temperature NOTICE If the incubator is operated at a temperature set below 4°C, the evaporator can ice up.
Page 66: Starting Manual Mode
Operation You can now either ■ select the existing value by tapping it and entering a new value via the keypad or ■ [Delete entry] button and delete the existing value with the entering a new value via the keypad. [Confirmation] button.
Page 67: Manual Mode Changes During Running Operation
Operation After a few seconds, the display will switch to the main screen. There, a countdown is displayed until the start of manual mode. NOTICE When the countdown is running, it is not pos- sible to change the manual mode settings. To start a program, first stop the countdown.
Page 68 Operation [Go back to the main You can return to the main screen via the screen] button. Fig. 91: Detailed manual mode screen [Go back to the main screen] button 68 / 173 12.2020 / Rev. 01 AB66006...
Page 69: Program Mode
Operation 6.12 Program mode 6.12.1 Program mode settings [Operating The settings for program mode can be reached via the modes] button or the HettCube activities area. Fig. 92: ‘Main screen  Settings’ [Operating modes] button HettCube activities area Fig. 93: Program mode settings Item Designation Description/function...
Page 70: Creating A Program
Operation Item Designation Description/function When this button is tapped, the currently activated program [Delete] button  Chapter 6.12.4 will be irrevocably deleted. ‘Copying and deleting a program’ on page 81 The program segments can be scrolled horizontally to the ‘Editing a Program segments ...
Page 71 Operation Editing the program name To edit the program name, proceed as follows: [Edit program name] button. Tap the  The editing window will open. Fig. 96: ‘Program mode  Creating a program  Editing the program name’ [Edit program name] button You can now either ■...
Page 72 Operation Tap on the program segment to be edited.  By tapping on the program segment to be edited, the selected program segment is highlighted in white in the pro- gram segment list. All other program segments are shown in grey.
Page 73 Operation Fig. 101: Program segment settings Item Designation Description/function [Confirmation] button After you have made all settings, you can confirm this with this button. Starting time Enter the starting time (date and time). The start time can only be defined for the first segment. Starting from the second segment, the start time is the end time of the pre- vious segment.
Page 74 Operation Item Designation Description/function You can change the temperature setpoint for this program ‘Set tempera- Set temperature  [Plus] and [Minus] buttons. segment in steps of 0.1 with the ture’ on page 65 By tapping on the temperature, a dialogue opens, via which you can enter the temperature setpoint directly.
Page 75 Operation Tap on the start time (can only be set for the first segment). Fig. 104: ‘Program segment settings Start time’  ‘Start time’ You can now either ■ select the existing values by tapping them and entering new values via the keypad or ■...
Page 76 Operation You can now either ■ select the existing values by tapping them and entering new values via the keypad or ■ [Delete entry] button and delete the existing values with the entering new values via the keypad. Enter the value in days, hours and minutes. Confirm the entries [Confirmation] button.
Page 77 Operation If an additional device is to be switched on in the interior or the ventilation opening is to be opened during the program seg- ment, you can activate the control contact for it here. Fig. 110: Activating the control contact [Activate/deactivate control con- tact] button Confirm the settings of the program segment by tapping on the...
Page 78: Editing A Program
Operation [Confirmation] button to save the program. Tap the Fig. 113: Saving a program [Confirmation] button [Cancel] button Confirm saving with the [Confirmation] button. Fig. 114: ‘Saving a program Confirmation’  [Confirmation] button  The saved program is displayed in the program overview. Fig.
Page 79 Operation Select the program to be edited from the program list.  The selected program is highlighted in white in the list. Fig. 116: ‘Program mode  Program overview’ Program list [Edit program] button [Edit program] button to edit the program. Tap the ...
Page 80 Operation [Duplicate program segment] button. Tap on the Fig. 118: Copying a program segment [Duplicate program segment] button  A duplicate is appended to the end of the program segment list. The copy is marked. The description for editing a pro- ‘Editing a program gram segment can be found under ...
Page 81: Copying And Deleting A Program
Operation 6.12.4 Copying and deleting a program Change to the ‘Program mode settings’ menu  Chapter 6.12.1 ‘Program mode settings’ on page 69, select the program to be edited from the program list and press the [Edit program] button. If you are already in the ‘Edit program’ menu, you can continue with editing the program name ‘Editing the program name’...
Page 82 Operation Copying a program To copy a program, proceed as follows: [Copy program] Select the program to be copied and tap the button. Fig. 122: ‘Program mode  Copying a program’ Program list [Copy program] button  The copy is created. Fig.
Page 83 Operation Select the program to be deleted from the program list and [Delete program] button. delete it by tapping the Fig. 124: ‘Program mode  Delete’ Program list [Delete program] button [Confirmation] button.  Confirm the deletion with the Fig. 125: ‘Program mode  Delete dialogue’...
Page 84: Program Mode - Start Settings
Operation 6.12.5 Program mode — Start settings Before starting program mode, you can change the start settings. Fig. 126: ‘Program mode  Start settings’ Item Designation Description/function [Start] button The currently selected program is started. Program behaviour The program behaviour indicates whether the times of the program segments start once the temperature of the pro- gram segment has been reached or after the specified time.
Page 85 Operation Item Designation Description/function The number of program repetitions can be entered here. [Program repetition] switch  ‘Pro- gram repetition’ on page 86 This function is for repeating the entire program. It is not possible to repeat individual program segments. In the case of program repetitions, it might make sense to integrate a standstill segment at the end of a program.
Page 86 Operation Timer To specify the starting point of the program, proceed as follows: Activate the timer.  The date and time for the program start can be specified. Tap on the date field.  The calendar will open. The current date is highlighted. Fig.
Page 87 Operation Activate the program repetition. Tap on the number of repetitions to set this. Fig. 131: Program repetitions [Program repetitions] switch ‘Number of repetitions’ specifica- tion You can now either ■ select the existing value by tapping it and entering a new value via the keypad or ■...
Page 88 Operation You can now either ■ select the existing value by tapping it and entering a new value via the keypad or ■ [Delete entry] button and delete the existing value with the entering a new value via the keypad. [Confirmation] button.
Page 89 Operation Program start The program cannot be started if another one is already being run. Starting a program ends manual mode. To start the program, proceed as follows: After the settings have been made, you can now start the pro- [Start] button.
Page 90: Program Mode Overview
Operation 6.12.6 Program mode overview In program mode, the temperature profile defined by you is run with the specified times. Fig. 139: Main screen Item Designation Description/function Call of manual and program mode. Chapter 6.11 Program settings  ‘Manual mode’ on page 54 ,  Chapter 6.12 ‘Program mode’...
Page 91 Operation A defined program runs in program mode. On the main screen, the rotating circle symbol, together with the symbol for manual mode, are displayed to indicate that temperature control is active. The number in the circle is the currently running pro- gram segment number of the program.
Page 92 Operation Fig. 141: Detailed program screen Item Designation Description/function Currently run program segment The number in the circle is the currently running program segment number of the program. Controller status Indicates the current controller status (heating or cooling). Program Currently running program. Actual temperature Indicates the current interior temperature of the incubator.
Page 93: Device Settings
Operation Item Designation Description/function Program segment information Indicates the temperature setpoint as well as the duration of the current program segment. The blue dot indicates the progress. 6.13 Device settings You can make basic incubator settings in the device settings. You can get to the device settings via the main screen.
Page 94 Operation If the incubator is in manual or program mode, not all setting options can be operated during the run; these are then greyed-out, such as the date and time. To use all setting options, the incubator must be in standstill mode. About Chapter 6.13.1 ‘About’...
Page 95: About
Operation 6.13.1 About In this menu, the contact data is displayed to you. Fig. 144: ‘About’ menu 6.13.2 Date & time In this menu, you can change the date and time of the control. Date and time are preset ex works and are changed during the initial installation if required.
Page 96: Temperature
Operation Tap on the displayed time. Fig. 147: ‘Date & time’ menu  The dialogue opens for editing the time. You can now either ■ select the existing values by tapping them and entering new values via the keypad or ■...
Page 97: Tolerance Band
Operation 6.13.4 Tolerance band In this menu, you can make the settings for the toler- ance band alarm. +3 K -5 K + 3 K - 5 K Fig. 150: Tolerance band. Temperature 2 Temperature 1 Alarm active (grey background on the diagram) Door opening - - - Temperature limit Temperature setpoint...
Page 98: Temperature Guard
Operation ■ The tolerance band, i.e. the actual temperature monitor, now engages when 40 °C is exceeded and 32 °C is fallen short of. ■ If the tolerance band is exceeded or fallen short of, an optical and acoustical alarm is output, and the potential-free alarm output is switched.
Page 99 Operation switched off; for class 3.2, the cooling. As soon as the actual tem- perature is back within the limits, the incubator will continue to regu- late. The temperature guards serve as device protection and sample protection. The temperature limits can limit the standard temperature range.
Page 100 Operation The setting ex works is: OFF and -10 °C. This temperature lies out- side of the temperature range of the incubator. The temperature specification value can be changed by tapping it. Fig. 154: ‘Temperature guard’ menu, class 3.2 [On] button is activated for 3.2, class 3.3 (class 3.1 and class If the 3.2) is activated automatically.
Page 101: Control Contact (Option)
Operation Fig. 158: Diagram of ‘Temperature guard’, class 3.3 A1 Target temperature 1 B1 Temperature specification, class 3.1 C1 Deviation from the target temperature starting from time C1 D1 Class 3.1 active starting from time D1 A2 Target temperature 2 B2 Temperature specification, class 3.2 C2 Deviation from the target temperature starting from time C2 D2 Class 3.2 active starting from time D2...
Page 102 Operation 6.13.6.1 Switchboard option The Switchboard option can be switched on/off with the control con- tact. "On" means that the Switchboard (external socket strip) is live. Fig. 159: ‘Control contact’ menu If the control contact is activated here, it can be used in manual and program mode.
Page 103 Operation For safety reasons, the incubator must be in opera- tion (manual or program mode) before the external device is started. This way, the external device is prevented from being switched on and energy (heat) prevented from being generated within the utilized space without the incubator being able to compen- sate for this.
Page 104 Operation energy, low temperatures might no longer be able to be reached. The lowest temperature that can still be reached can be found in the heat Chapter 6.14 ‘Heat compensation’ compensation diagram  on page 118 . Configure the external device such that it starts when the supply voltage is switched on.
Page 105: Language
Operation NOTICE − If the ventilation opening is opened during cooling mode, there is a danger of moisture from the envi- ronment getting into the incubator. − At target temperatures below 10 °C, the ventila- tion opening must be kept closed. Otherwise, there is a danger the evaporator will ice up.
Page 106: Door
Operation 6.13.9 Door If the door is opened, an information banner immediately appears indicating the duration the door is opened. The duration of the door being opened is updated in real time. The incubator calls attention to the fact that the temperature is changing in the incubator due to the open door;...
Page 107 Operation Sleep mode To set sleep mode, proceed as follows: Tap on the sleep mode value. You can now either ■ select the existing value by tapping it and entering a new value via the keypad or ■ [Delete entry] button and delete the existing value with the entering a new value via the keypad.
Page 108: Power Failure
Operation Activating the screen To activate the screen, proceed as follows: Tap on the screen to reactivate this. You will be prompted to enter the Admin PIN. If you have forgotten your PIN in this state, you can reset the [Bit key] button ...
Page 109: Failure Alarm
Operation 6.13.12 Failure alarm You can configure the alarm sound with this setting. The volume, fre- quency and duration are adjustable. The volume of the failure alarm can be adjusted in stages. ‘Number of reproduced signal sounds’ , it can be speci- Via the input Fig.
Page 110 Operation Select where the data to be exported should be saved and [Confirmation] button. confirm the selection with the Device directory In the root directory of the USB stick, a folder is automatically created during the export which has the incubator serial number as its name.
Page 111 Operation [Confirmation] button. Confirm the message with the  You will go back to the Export menu. Fig. 180: Export finished [Confirmation] button [Eject USB stick] button in order to be able to You can tap the remove the USB stick. Fig.
Page 112: Import
Operation 6.13.14 Import Data import is only possible in standstill mode Fig. 183: ‘Import’ menu Via the import function, you can import the HettCube programs and HettCube temperature drops from a USB stick. For service technicians, additional import and export functions are available.
Page 113 Operation If a program with the same name already exists, you can select how you would like to further proceed. If there is no program with an identical name, continue with the next step. Select one of the two options Keep both A copy is made of the imported pro- gram.
Page 114: Hours Of Operation
Operation 6.13.15 Hours of operation In this menu, the individual operating hours are dis- played to you. Fig. 191: ‘Operating hours’ menu The operating hours of the individual assemblies/functions are dis- played. 6.13.16 Logbook There are the following options for calling up the log- book: −...
Page 115: System Information
Fig. 194: ‘System information’ menu The system information contains information about the version num- bers of the device components. In addition, the serial numbers and contact data of Andreas Hettich GmbH & Co.KG are displayed. 6.13.18 Admin access [Change admin PIN]...
Page 116 Operation Enter the new admin PIN via the keypad and confirm the input [Confirmation] button. with the Fig. 197: Entering a new PIN [Confirmation] button Keypad Enter the new admin PIN again via the keypad and confirm the [Confirmation] button. input with the ...
Page 117 Operation Confirm that you would like to enter the PUK by tapping on the [Confirmation] button. Fig. 200: Confirming entry of PUK [Confirmation] button Enter the 4-digit PUK via the keypad and confirm the input with [Confirmation] button. Fig. 201: PUK entry Keypad [Confirmation] button ...
Page 118: Service Dashboard
Operation [Confirmation] button to accept the PUK as the new Tap the ‘Change admin admin PIN. The admin PIN can be changed  PIN’ on page 115 . Fig. 204: PUK confirmation [Confirmation] button 6.13.19 Service dashboard Personnel: ■ Service technician Fig.
Page 119 °C) Lowest reachable temperature, HettCube 400 R (0 °C to +65 °C) Lowest reachable temperature, HettCube 200 R (0 °C to +65 °C) The lowest reachable temperature values when using external devices with different heat outputs in the interior of the incubator.
Page 120: Cleaning, Disinfection And Maintenance
Cleaning, disinfection and maintenance Cleaning, disinfection and maintenance DANGER A danger is posed by penetrating liquids. There is an electric shock hazard for personnel. WARNING There is a risk of contamination of the microbiological sample material. If cleaning and/or disinfection are not sufficient after contamination, there is a risk that microbiological sample material will be contaminated with foreign germs.
Page 121: Cleaning
Cleaning, disinfection and maintenance In the event of strong contamination, the filter mat must be exchanged. A service technician must do the exchanging. For incubators with cooling, we recommend that the filter mat be exchanged once a year. Cleaning Surface cleaning and care WARNING The incubator might be contaminated.
Page 122 Cleaning, disinfection and maintenance Disengage the support rails upward and out of the front latching rail and then pull out toward the front from the rear latching rail. Fig. 208: Removing the support rails Hold onto the baffle sheet and screw out both knurled screws. Fig.
Page 123: Disinfection
Cleaning, disinfection and maintenance Disengage the right baffle sheet upward and then remove toward the front. Installation is done in the opposite order. When installing the upper baffle sheet, push this upward and fasten with the two knurled screws. It must be flush with the ceiling of the interior.
Page 124: Autoclaving
Cleaning, disinfection and maintenance Autoclaving Autoclaving accelerates the ageing process of plas- tics. In addition, it can cause discolourations in plas- tics. The slide-in modules, drawers, support rails, telescope rails, latching rails and plates in the interior can be autoclaved at 121°C / 250°F (20 Min).
Page 125: Troubleshooting
Troubleshooting Troubleshooting If the error cannot be remedied using the trouble- shooting table, notify the service technician. Please specify the type of device and the serial number. Both numbers can be found on the name plate of the incubator. If a malfunction occurs, there is an optical and acoustic alarm.
Page 126: Warnings And Error Messages
Troubleshooting Warnings and error messages When a malfunction occurs, there is an optical and acoustic alarm and the potential-free alarm contact switches. Additional signal encoder for monitoring the con- troller unit: Alarms, which are sent from the operating unit or controller unit, are placed at the potential-free alarm output and can be connected to external signalling equipment (acoustic or visual) by the customer.
Page 127 Troubleshooting Fault description Cause Remedy If the error appears again: Call Service; Provide the device type, serial number and error number. Error 3 Sensor measured value below Call Service; Provide the device the valid measuring range. type, serial number and error number.
Page 128 Troubleshooting Fault description Cause Remedy Error 19 Memory issue Call Service; Provide the device type, serial number and error number. Error 20 Memory issue Call Service; Provide the device type, serial number and error number. Error 21 Memory issue Call Service; Provide the device type, serial number and error number.
Page 129 Troubleshooting Fault description Cause Remedy Error 30 This is not an error, but a Please acknowledge the message warning for the user that there with [X] ; the set operating mode was a power failure. The pro- continues. gram/manual mode is con- tinued based on the user set- tings (Tolerance band and Power failure/Tolerance band).
Page 130 Troubleshooting Fault description Cause Remedy Error 37 The temperature set in temper- Please acknowledge the message ature guard 3.2 was exceeded. with [X] ; The customer decides The set temperature setpoint is what to do next after checking the higher than the set temperature samples.
Page 131: Door Warning
Troubleshooting Fault description Cause Remedy Error 50 The temperature difference Please acknowledge the message [X] ; The customer decides between the two independent with temperature sensors was too what to do next after checking the great. samples. If the error appears again: Call Service;...
Page 132: Event Overview
Troubleshooting 8.2.2 Event overview The events which have occurred while the program/manual mode ‘Status information’ bar. were running are shown collectively in a blue [Logbook] button, an overview of the events, such If you tap on the as the door opening/closing, program pause, program continuation, [Logbook] button are displayed with the start time and duration.
Page 133: Technical Data
Technical data Technical data HettCube 200 Manufacturer Andreas Hettich GmbH & Co. KG, D-78532 Tuttlingen Model HettCube 200 Type 62001 62001-01 Mains voltage (± 10 %) 220-240 V 1~ 100-120 V 1~ Mains frequency 50-60 Hz Connected load 450 VA/850 VA 500 VA/900 VA Energy consumption at 37°C...
Page 134: Hettcube 200 R
All temperature data was measured at an ambient temperature of 22°C and in acc. with DIN 12880:2007-05. The data applies to incu- bators with standard equipment. HettCube 200 R Manufacturer Andreas Hettich GmbH & Co. KG, D-78532 Tuttlingen Model HettCube 200 R Type 62006 62006-01 Mains voltage (±...
Page 135 Technical data Global-warming potential (GWP) Inner volume 150 l Ambient conditions (EN / IEC 61010-1) Set-up site Indoors only Height Up to 2000 m above sea level Ambient temperature 16°C to 35°C Humidity Maximum relative humidity 75%, non-condensing. Overvoltage category (IEC 60364-4-443) Degree of contamination Device protection class...
Page 136: Hettcube 400
All temperature data was measured at an ambient temperature of 22°C and in acc. with DIN 12880:2007-05. The data applies to incu- bators with standard equipment. HettCube 400 Manufacturer Andreas Hettich GmbH & Co. KG, D-78532 Tuttlingen Model HettCube 400 Type 64001 64001-01 Mains voltage (±...
Page 137: Hettcube 400 R
All temperature data was measured at an ambient temperature of 22°C and in acc. with DIN 12880:2007-05. The data applies to incu- bators with standard equipment. HettCube 400 R Manufacturer Andreas Hettich GmbH & Co. KG, D-78532 Tuttlingen Model HettCube 400 R Type 64006...
Page 138 Technical data Mains voltage (± 10 %) 220-240 V 1~ 100-120 V 1~ Mains frequency 50-60 Hz Connected load 450 VA/850 VA 500 VA/900 VA Energy consumption at 37°C 0.046 kWh/h Cooling medium R 513 a (Contains fluorinated greenhouse gases documented by the Kyoto Pro- tocol) Amount of refrigerant 200 g...
Page 139: Hettcube 600
All temperature data was measured at an ambient temperature of 22°C and in acc. with DIN 12880:2007-05. The data applies to incu- bators with standard equipment. HettCube 600 Manufacturer Andreas Hettich GmbH & Co. KG, D-78532 Tuttlingen Model HettCube 600 Type 66001 66001-01 Mains voltage (±...
Page 140 Technical data Ambient temperature 16°C to 35°C Humidity Maximum relative humidity 75%, non-condensing. Overvoltage category (IEC 60364-4-443) Degree of contamination Device protection class Noise level ≤ 41 dB(A) Not suitable for use in explosion-endangered areas. Emitted interference, immunity to EN/IEC 61326-2-6, Class B interference Type of protection (EN 60529) IP 20...
Page 141: Hettcube 600 R
All temperature data was measured at an ambient temperature of 22°C and in acc. with DIN 12880:2007-05. The data applies to incu- bators with standard equipment. HettCube 600 R Manufacturer Andreas Hettich GmbH & Co. KG, D-78532 Tuttlingen Model HettCube 600 R Type 66006 66006-01 Mains voltage (±...
Page 142 Technical data Emitted interference, immunity to EN/IEC 61326-2-6, Class B interference Type of protection (EN 60529) IP 20 Temperatures Temperature range 0 °C to 65 °C Temperature setting precision 0.1°C Temperature deviation over time at ± 0.1 K 37 °C Spatial temperature deviation at ±...
Page 143: Definition Of The Utilized Space
Technical data Definition of the utilized space Fig. 215: Utilized space Interior 600/600 R 400/400 R 200/200 R C (in mm) D (in mm) E (in mm) 1415 Interior, rounded (in litres) Tab. 3: Interior Utilized space position 600/600 R 400/400 R 200/200 R above sensor...
Page 144: Type Plate
Technical data Utilized space position 600/600 R 400/400 R 200/200 R above sensor Utilized space (in litres) Tab. 4: Utilized space in acc. with DIN 12880:2007-05 Type plate Fig. 216: Type plate Manufacturer logo Product designation Type number Serial number possibly IVD symbol possibly EAC marking, CE marking Country of manufacture...
Page 145: Dimensions
Technical data Dimensions Standard dimensions *535 *690 Fig. 217: Dimensions Interior Door opening ≤ 90°, self-closing HettCube 200 / HettCube 400 / HettCube 600 / 200 R 400 R 600 R min. 970 mm min. 1425 mm min. 1990 mm...
Page 146 Technical data ‘Lateral access Dimension for option ±5 port’ Fig. 218: ‘Lateral access port’ dimension Æ22 mm Æ42 mm Æ67 mm X ±5 26 mm 33 mm 40 mm 146 / 173 12.2020 / Rev. 01 AB66006...
Page 147: Disposal
Disposal Disposal Before disposal, the incubator must be decontaminated and cleaned to protect personnel, the environment and property. When disposing of the incubator, the respective legal regulations must be observed. According to the directive 2012/19/EU , devices may no longer be disposed of in the household waste.
Page 148: Free And Open Source Software
License Terms and Conditions regarding LGPL components: For those parts of the HETTICH software that are licenses under ■ the "GNU Library General Public License" or the "GNU Lesser General Public License"...
Page 149: Index
Index Index About........95 Editing a program..... . . 78, 81 Accessories.
Page 150 Program mode......90 HettCube 200......133 Program mode —...
Page 151 Index Warning symbols......7 Warnings and error messages....126 AB66006 12.2020 / Rev.
Page 152 Index 152 / 173 12.2020 / Rev. 01 AB66006...
Page 153: 13 Appendix
13 Appendix...
Page 155: A Software Symbols
Software symbols Software symbols Designation Icon After power failure, continue program. After power failure, hold holding temperature. Upper temperature guard limit (3.1) Lower temperature guard limit (3.2) Cancel Alarm muted Editing Starts on Required time until end Calculated program end Screen lock in sleep mode Date Settings...
Page 156 Software symbols Designation Icon Brightness Logbook Up/down Information Interior temperature Copy Cooling Volume List view Delete Minus To the left… To the right… OK/Accept Pause Pause Resume program Plus Program mode Program repetition counter 156 / 173 12.2020 / Rev. 01 AB66006...
Page 157 Software symbols Designation Icon Operating modes Delete input Switchpoint Control contact (external contact) active Close Sleep mode Service settings Holding temperature at end of process Start Stop Increase temperature Decrease temperature Temperature drop Set timer time Timer value Tolerance band. Sound switched off Door warning Door opened...
Page 158 Software symbols Designation Icon Toggle switch Next Go back Go back to the main screen Reset admin PIN 158 / 173 12.2020 / Rev. 01 AB66006...
Page 159: B Examples
Examples Examples Customer examples and practical In manual mode: Temperature drop wishes ■ During the entire weekend (Friday afternoon to Monday morning) ■ Over one day (Sunday) ■ With holiday function (using Wednesday as example) ■ Over the weekend with start delay In program mode ■...
Page 160: Temperature Drop Over One Day (Sunday)
Examples Temperature drop over one day (Sunday) °C Fig. 220: Diagram ‘Temperature drop over one day (Sunday)’ Operating mode: Manual mode; Temperature drop via real-time calendar Procedure: In manual mode, set the temperature drop to 4°C from Sat- urday night at midnight to Monday morning at 3:00 a.m. and ‘weekly’...
Page 161: Temperature Drop On The Weekend With Start Delay
Examples In manual mode, set the temperature drop to 4°C from Friday afternoon at 2:00 p.m. to Monday morning at 3:00 a.m. and ‘weekly’  ‘Creating a temperature set the repetition interval to drop’ on page 60 . Set another temperature drop from Tuesday evening at 20:00 p.m.
Page 162: Temperature Drop On The Weekend Including The Passive Dehumidification Option
Examples Temperature drop on the weekend including the passive dehumidification option Description: The incubator is operated at a temperature of 37 °C during the work- week. Starting on Saturday 12:00 p.m., the temperature drops to 4 °C. On the following Monday, the sample material is taken out at approx.
Page 163: External Devices Are Integrated In Program Mode, E.g. Day/Night Simulation
Examples External devices are integrated in program mode, e.g. DAY/NIGHT simulation. Customer wish/description: Customer would like to simulate the ambient conditions (light and temperature) for its organisms (e.g. plants) daily. The samples should be charged as follows: ■ The day is simulated with +22 °C and 16 "daylight hours". ■...
Page 164: Combination Of Standstill Mode With Incubation Mode (Energy-Saving Mode)
Examples Set the program as shown  Chapter 6.12.2 ‘Cre- ating a program’ on page 70. Please take the starting time into account  ‘Timer’ on page 86. Fig. 227: ‘Day/night simulation ’program Combination of standstill mode with incubation mode (energy-saving mode) °C Fig.
Page 165: Incubator Is Always Cleaned On Wednesdays Due To The Laboratory Routine (Sop) (Standstill Mode)
Examples Incubator is always cleaned on Wednesdays due to the laboratory routine (SOP) (standstill mode) °C Fig. 230: Diagram ‘Incubator is always cleaned on Wednesdays due to the laboratory routine (SOP) (standstill mode)’ Starting time (Thursday, 12:00 a.m.) PS Program segment Operating mode: Program mode;...
Page 166 Examples 166 / 173 12.2020 / Rev. 01 AB66006...
Page 167: C Accessory Overview
60029 Glass door All-glass outer door, for HettCube 400 | 400 R 60030 Glass door All-glass outer door, for HettCube 200 | 200 R 60001 Shelf (set) Made of stainless steel, with standard rails, max. load (kg): 50 60031 Shelf (HTS set) Made of stainless steel, with telescopic rails, pull out up to 70 %, max.
Page 168 Æ (mm): 22, foam stoppers 60007 Access port Æ (mm): 42, foam stoppers 60008 Access port Æ (mm): 67, foam stoppers 60009 Stacking kit For safe stacking of two HettCube 200 | 200 Rs 168 / 173 12.2020 / Rev. 01 AB66006...
Page 169 60010 Rolling cabinet Lockable, with one drawer, incl. lockable castors, W x D x H (mm): 770 x 500 x 550, for HettCube 200 | 200 R 60919 Fixing kit (HTS set) For fixing of shelves and drawers with telescopic rails, made of polyamide AB66006 12.2020 / Rev.
Page 170 Accessory overview 170 / 173 12.2020 / Rev. 01 AB66006...
Page 171: D Declaration Of Conformity
Declaration of conformity Declaration of conformity AB66006 12.2020 / Rev. 01 171 / 173...
Page 172 Type of device: Incubator / cooling incubator Type designation: HettCube 200 / 200 R, HettCube 400 / 400 R, HettCube 600 / 600 R The above-named incubator/cooling incubator meets the relevant harmonization legislation of the Union: • Low Voltage Directive 2014/35/EU •...
Page 173: E List Of Standards
List of standards List of standards Standards and regulations which The device is a very high-tech product. It is subject to extensive apply to this device testing and certification procedures according to the following stand- ards and regulations in their respectively valid version: Electrical and mechanical safety for Standard series: IEC 61010 (corresponds to standard series DIN EN construction and final inspection:...

This manual is also suitable for:

Hettcube 200 r Hettcube 400 Hettcube 400 r Hettcube 600 Hettcube 600 r

Hettich HettCube 200 Operating Manual

1 Introduction

2 Safety Information

3 Device Description

4 Transport and Storage

5 Commissioning

6 Operation

7 Cleaning, Disinfection and Maintenance

8 Troubleshooting

9 Technical Data

10 Disposal

11 Free and Open Source Software

12 Index

13 Appendix

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