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Oxygraph-2k Manual
Mitochondrial Physiology Network 19.01(B01): 19-24 (2014)
B:
www.bioblast.at/index.php/MiPNet19.01B_POS-Service
Service of the
polarographic
oxygen sensor
OroboPOS
Gnaiger E
OROBOROS INSTRUMENTS Corp
high-resolution respirometry
Schöpfstr 18, A-6020 Innsbruck, Austria
Email:
erich.gnaiger@oroboros.at
www.oroboros.at
Section
1.
Accessories ............................................. 20
2.
Cleaning ................................................. 20
3.
Membrane mounting ................................ 22
4.
Electrical cable connection ........................ 23
5.
Storage of the POS .................................. 23
6.
Replacement of the POS head ................... 24
7.
References .............................................. 24
Summary:
Service of the polarographic oxygen sensors (OroboPOS,
POS) is the basis for signal stability, low noise and high time resolution.
Sensor service may not be required for several months of operation.
Performance of the OROBOROS Oxygraph-2k according to instrumental
specifications is obtained only with oxygen sensors which are maintained
in a good functional state.
level of zero current (0% to max. 5%). For each sensor, the frequency of
POS service can be optimized on the basis of a long-term record of
calibration values as a quality control (O2k-Calibration-List.xls).
instruments@oroboros.at
POS service is required if (1) a new
sensor is prepared, (2) the raw
signal at air calibration declines, (3)
signal noise is high, (4) the time
response
is
prolonged
constant >10 s) and biphasic, (5)
the oxygen signal at zero calibration
does not decline rapidly to a low
2014 OROBOROS
Version B01: 2014-03-06
Page
(time
www.oroboros.at
B
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Related Manuals for Oroboros Instruments Oxygraph-2k
Summary of Contents for Oroboros Instruments Oxygraph-2k
- Page 1 POS) is the basis for signal stability, low noise and high time resolution. Sensor service may not be required for several months of operation. Performance of the OROBOROS Oxygraph-2k according to instrumental specifications is obtained only with oxygen sensors which are maintained in a good functional state.
- Page 2 For cleaning of the anode and cathode, sensor head best Figure 2. Removal of a used removed from the OroboPOS-Connector membrane from the POS. OROBOROS Oxygraph-2k OROBOROS INSTRUMENTS...
- Page 3 (overnight), sealing the ammonia under a membrane and under the POS cover slip in long-term applications. Protect the POS from light, since the silver/silver chloride anode is light sensitive. O2k-Core Manual OROBOROS INSTRUMENTS...
- Page 4 (Fig. 4 [3]). Then slide the membrane ring holder into the membrane guide and press firmly down to slide the PEEK membrane ring over the POS head (Fig. 4 [4]). OROBOROS Oxygraph-2k OROBOROS INSTRUMENTS...
- Page 5 5.1. Short-term storage in the Oxygraph-2k For short periods of days or several weeks, the POS is stored in the Oxygraph-2k chamber. The chamber is washed with distilled water and completely filled with 70% ethanol for chemical sterilization. The stopper is inserted loosely without pushing it down beyond the point where the sealing ring is inserted into the glass chamber.
- Page 6 Hitchman ML, Gnaiger E (1983) A thermodynamic consideration of permeability coefficients of membranes. In: Polarographic oxygen sensors, Gnaiger E and Forstner H, eds., Springer, Berlin, Heidelberg, New York: 31-36. » Full version » www.bioblast.at/index.php/MiPNet19.01B_POS-Service Next step – O2k-Core Manual D » MiPNet19.01D O2k-Calibration OROBOROS Oxygraph-2k OROBOROS INSTRUMENTS...
- Page 7 POS cable into the female plug positioned between the two control lights of the Oxygraph-2k (left chamber: between the MAINS and STIRRER lights; right chamber: between the STIRRER and COMM lights). The ridge on the male plug has to face accurately...
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