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SAS-3
Operator Manual
English
1
HL-2-1514P 2017-01(4) [SAS-3 Operators Manual] EN.indd 1
11/01/2017 10:23

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Summary of Contents for helena SAS-3

  • Page 1 SAS-3 Operator Manual English HL-2-1514P 2017-01(4) [SAS-3 Operators Manual] EN.indd 1 11/01/2017 10:23...
  • Page 2 SAS-3 Operator Manual HL-2-1514P 2017-01(4) [SAS-3 Operators Manual] EN.indd 2 11/01/2017 10:23...
  • Page 3: Table Of Contents

    6.2 SAS-3 Carbon Electrode 6.3 SAS-3 Reagent Spreader 6.4 SAS-3 Antisera Template 6.5 SAS-3 60 Sample Tray 6.6 Cleaning Spills 6.7 Replace fuse(s) 6.8 Replacing Contact Sheet 6.9 Lubrication of hinges HL-2-1514P 2017-01(4) [SAS-3 Operators Manual] EN.indd 3 11/01/2017 10:23...
  • Page 4 SAS-3 Operator Manual Troubleshooting Performance Specification 17 Warranty WEEE HL-2-1514P 2017-01(4) [SAS-3 Operators Manual] EN.indd 4 11/01/2017 10:23...
  • Page 5: The Sas-3

    This manual provides all necessary information for the setup and operation of the SAS-3 including important information regarding safety and maintenance of the unit. To ensure safe and reliable operation, always operate the SAS-3 in accordance with the operator manual.
  • Page 6: Safety Considerations

    The SAS-3 is fully overload protected. However, the following guidelines should be observed to ensure safe operation of the unit. The SAS-3 must only be operated when connected to an earthed supply ■ of the correct voltage and frequency as indicated on the serial number label.
  • Page 7: Installation

    SAS-3 Gel Block Remover 311100 SAS-3 Antisera Template 311000 Place the SAS-3 unit on a level bench. Keep the area around the ■ instrument clear to ensure correct ventilation. Select an environment free from excessive humidity, dust and large ■...
  • Page 8: Controls

    Applicator Assembly The applicator assembly holds the required number of SAS-3 Applicators. It can move left and right to allow positioning of sample tray and up and down when loading and applying samples. Sample Base...
  • Page 9: Operating Instructions

    Connect the unit to an appropriate mains outlet and switch on by using the power switch located on the left side of the instrument. The display will show the Helena Biosciences Europe logo. This shows that the machine is ready to operate.:...
  • Page 10: Create Or Edit A Program

    Apply sample The applicator contacts the gel for a specified time thereby applying the sample on the gel. Electrophoresis The gel will be subjected to a high voltage during a specified time. Apply reagent When appropriate the gel will be provided with a reagent that is spread with multiple spreading movements. HL-2-1514P 2017-01(4) [SAS-3 Operators Manual] EN.indd 10 11/01/2017 10:23...
  • Page 11: View Process Parameters During Program Run

    Abort a program run To abort a program at any stage, press START/STOP. NOTE: It is not possible to pause any stages of the program. The gel can then be removed. HL-2-1514P 2017-01(4) [SAS-3 Operators Manual] EN.indd 11 11/01/2017 10:23...
  • Page 12: Options Menu

    SAS-3 Operator Manual Options Menu The SAS-3 is provided with an Options Menu giving access to a number of instrument settings. When the display shows Helena Biosciences Europe the Options Menu can be reached by pushing the ENTER and START/STOP button simultaneously.
  • Page 13: Maintenance

    Do not use corrosive or abrasive cleaners. Dry the unit before plugging the power cord in. SAS-3 Carbon Electrode After each use, rinse each SAS-3 Carbon Electrode in deionised water and dry with a lint-free tissue. SAS-3 Reagent Spreader After each use, rinse each SAS-3 Reagent Spreader with deionised water and dry with a lint-free tissue.
  • Page 14: Replacing Contact Sheet

    Let the unit stand for 5 minutes to evaporate all remaining residue. When finished turn off the power and unplug the cord. Take a new SAS-3 contact sheet. On the side punched with two holes, peel the backing off for about 1-2 cm and fold back. This stiffens the side. Align the new contact sheet, adhesive side down, over the two alignment pins so that the two holes will fit over the two round pins. Rub the edge...
  • Page 15: Lubrication Of Hinges

    When the lid does not close correctly, or when it needs too much force to open, it is advised to apply a small drop of oil on each of the hinges. HL-2-1514P 2017-01(4) [SAS-3 Operators Manual] EN.indd 15 11/01/2017 10:23...
  • Page 16 Check and/ or replace contact sheet Check and/or replace electrodes Contact your local distributor Electrophoresis Remove possible heating/cooling Restricted fan air flow obstructions from the air problems inlet Contact your local In all other cases distributor HL-2-1514P 2017-01(4) [SAS-3 Operators Manual] EN.indd 16 11/01/2017 10:23...
  • Page 17 10 - 62°C (minimum step size 1°C) Operating Temperature: 15 - 27°C ambient temperature Environmental 90% Rh Conditions: Dimensions: Weight: 36 kg Size: 530mm (W) x 270mm (H) x 600mm (D) HL-2-1514P 2017-01(4) [SAS-3 Operators Manual] EN.indd 17 11/01/2017 10:23...
  • Page 18 SAS-3 Operator Manual Warranty Helena warrants each unit to be free from defects in material and workmanship. Helena’s liability under this warranty is limited to servicing or adjusting any unit and to replacing any defective part. This warranty is effective for 1 year commencing from the date of shipment to the original purchaser, and does not cover faults caused by misuse, abnormal conditions, damage incurred in shipment, or consumable items such as lamps.
  • Page 19 HL-2-1514P 2017-01(4) [SAS-3 Operators Manual] EN.indd 19 11/01/2017 10:23...
  • Page 20 Helena Biosciences Europe Queensway South Team Valley Trading Estate Gateshead Tyne and Wear NE11 0SD United Kingdom Tel: +44 (0)191 482 8440 Fax: +44 (0)191 482 8442 Email: info@helena-biosciences.com HL-2-1514P 2017/01 (4) HL-2-1514P 2017-01(4) [SAS-3 Operators Manual] EN.indd 20 11/01/2017 10:23...

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