Related Manuals for Amplyus miniPCR mini16
Summary of Contents for Amplyus miniPCR mini16
- Page 1 User’s guide (c)2013-2019 Amplyus LLC. miniPCR bio and the miniPCR bio logo are trademarks of Amplyus LLC mini16 user’s guide support@minipcr.com...
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Page 2: Table Of Contents
INDEX Quick start guide Warranty Specifications Features Components Software Overview Contents Optional accessories Getting started Download Connect Software operation Protocol types Create and save Editing protocols Running and monitoring protocols Operation without software Hardware operation Loading your samples Quickguide to LED interface Troubleshooting and maintenance Troubleshooting Care &... - Page 3 QUICK START GUIDE 1 -- Download software from minipcr.com/downloads 2 -- Connect mini16 to your device using USB or Bluetooth > Devices tab 3 -- Library tab Create a PCR, heat block, linear ramp or Flex program > 4 -- Run by clicking the play symbol to upload the program and start the run 5 --...
- Page 4 Amplyus’ sole liability, under this Limited Warranty, for failure to repair or replace miniPCR thermal cyclers after a reasonable number of attempts, is limited to the replacement of the Product or, at Amplyus’ sole discretion, the refund of the original purchase price of the Product.
- Page 5 SPECIFICATIONS FEATURES Dual Bluetooth® and USB conectivity Programmable via Windows, Mac, iPhone, iPad, Android and Kindle Fire PCR, touchdown PCR, heat block, linear ramp and FLEX programming modes FLEX mode allows for arbitrary combinations of up to 15 programming steps Portable and durable construction 16 x 0.2 ml PCR tube capacity (strip compatible) On-device microprocessor runs autonomously...
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Page 6: Optional Accessories
CONTENTS miniPCR mini16 thermal cycler Universal voltage power adapter (2AA73) US-style AC cord (QP-1000-07) 6 ft USB Cable (QP-1000-09) Drawstring travel pouch (QP-1000-12) OPTIONAL ACCESSORIES miniPCR™ Power Pack, 20,000 mAh Li-Ion Battery (QP-1000-13) Portable solar panel (contact team@minipcr.com) support@minipcrbio.com mini16 user’s guide... -
Page 7: Getting Started
GETTING STARTED DOWNLOAD THE miniPCR (FREE DOWNLOAD) miniPCR App v2.0 or higher is needed to run mini16. 1 -- Visit www.minipcr.com/downloads and select your platform of choice (Windows, Mac, iOS, Android, Amazon Kindle Fire). Minimum system requirements are listed on the website. -
Page 8: Connect
CONNECT Establishing a Bluetooth (BLE) connection (iOS, Windows, Mac, Android and Kindle Fire) Note: make sure Bluetooth is turned on on your device. 1 -- Turn on the mini16 using the on/off switch on the back of the unit. A flashing blue LED on the front of the mini16 indicates that it is ready to connect to your device. - Page 9 CONNECT (continued) Establishing a USB connection (Windows, Mac, Android and Kindle Fire) 1 -- USB-C USB-C to USB-A adapter required. Android and Kindle Fire 1. With software closed, connect the mini16 to your Android or Kindle Fire using a USB-C to USB-A adapter and the original USB cable provided with the miniPCR.
- Page 10 OPTIONAL CONFIGURATIONS Establishing connections to multiple miniPCR units This procedure works for connecting units through BLE and/or USB. 1 -- Go to Devices to see the list of available mini16 units. Note: it is possible to connect mini8 and mini16 units simultaneously. 2 -- All units connected through USB will automatically appear as ‘Connected’...
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Page 11: Software Operation
SOFTWARE OPERATION miniPCR App v2.0 or higher is needed to run mini16. CREATING AND SAVING PROTOCOLS You can program mini16 in four different modes: PCR, heat block, linear ramp and FLEX (includes touchdown PCR) 1 -- Open the miniPCR™ App in your device and remain on the “Library” tab 2 -- Click on the button on the top right corner. - Page 12 CREATING AND SAVING PROTOCOLS (cont.) PCR mode Input temperatures up to 99 °C, step times up to 9999 seconds minutes and cycles up to 99. Heated lid is turned on by default. This setting mantains the lid at approx 105 °C to prevent condensation on the caps of the tubes.
- Page 13 CREATING AND SAVING PROTOCOLS (cont.) Linear ramp mode Select the initial and final temperatures and the ramp time from the first to the second temperature. A heating or cooling ramp can be selected. Heated lid is turned on by default. This setting mantains the lid at approx 10 degrees above the current temperature.
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Page 14: Editing Protocols
PROTOCOL OPTIONS Editing protocols 1 -- Click on the desired protocol from your Library to show available options: Run, View/ Edit, Copy, Delete. Click View/Edit. Win/Mac Mobile / iOS / Amazon Fire Copying protocols 2 -- Update protocol parameters (e.g. change the number of cycles, temperature, etc.) 3 -- Click “Save Only”... -
Page 15: Running And Monitoring Protocols
RUNNING AND MONITORING Starting the run 1 -- Move the power switch on the back of the mini16 to the ON position. 2 -- Connect your miniPCR thermal cycler via USB cable or Bluetooth. Tip: You can simultaneously monitor multiple miniPCR machines with one device. Each miniPCR will appear as a separate tab in the “Now running”... - Page 16 Autonomous run feature • It is not necessary to maintain a USB or Bluetooth connection during a run. The miniPCR will keep running the protocol while the power switch remains in the ON position. • Toggling the power switch OFF and ON will restart the last protocol programmed (with Auto Start ON, see below).
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Page 17: Hardware Operation
HARDWARE OPERATION LOADING YOUR SAMPLES 1 -- Open the lid by pinching the side tabs on the front plate of your miniPCR machine (indicated by arrows). Use your thumb and index fingers to pinch, and lift the lid with your other hand. Load the PCR tubes in the metal block. Pinch both tabs to release lid 2 -- Fully untwist (counterclockwise) the adjustment knob and press the lid down until the... -
Page 18: Quickguide To Led Interface
QUICK GUIDE TO LED INTERFACE All LEDs flashing: protocol uploaded or device initializing Blue LED flashing: mini16 available for Bluetooth pairing Blue LED on: mini16 paired via Bluetooth Green, Yellow, and Red LEDs cycling: mini16 ready for protocol run. Press “Run” to start All LEDs on: protocol complete Yellow LED flashing: error,... -
Page 19: Troubleshooting And Maintenance
TROUBLESHOOTING AND MAINTENANCE TROUBLESHOOTING Lid won’t latch: ensure tubes are properly inserted into block and lid is at maximum height (turn knob counterclockwise). Run won’t start: ensure power supply is plugged into AC outlet and the power supply LED is on. Check miniPCR power switch is in the ON position. Ensure protocol uploads and LEDs blink. -
Page 20: Care & Maintenance
CARE & MAINTENANCE miniPCR thermal cyclers do not require regular maintenance. They can be cleaned using water or an acrylic cleaning agent. They should not come into contact with organic solvents or corrosive solutions. Ensure that no liquid enters the device. For safety reasons, the device must be switched off and disconnected from the power supply before cleaning. -
Page 21: Operating Conditions
OPERATING CONDITIONS miniPCR thermal cyclers are designed to be safe to operate under the following conditions: • Indoor use • Altitude up to 2,000 m • Temperature 9 oC to 40 °C • Maximum relative humidity 80% for temperatures up to 31 °C, decreasing linearly to 50% relative humidity at 40 °C •...
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