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GC Column Care Guide
GC Column Installation............................................................................................. 1-2
GC Column Conditioning and Testing ........................................................................... 3
Protecting the GC Column and Extending Life Time .................................................. 4
Carrier Gas Selection and Flow Optimization ............................................................ 5
Column Storage .................................................................................................... 5
Capillary Columns ..................... 6
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Summary of Contents for Phenomenex GC
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Page 1: Table Of Contents
GC Column Installation..................... 1-2 GC Column Conditioning and Testing ................3 GC Hints and Tips Protecting the GC Column and Extending Life Time ..........4 Carrier Gas Selection and Flow Optimization ............5 Column Storage ....................5 Chemical Compatibility / Solvent Rinsing of Zebron Capillary Columns ..... -
Page 2: Gc Column Installation
GC Column Installation A. Capillary GC Installation with a Traditional GC Nut Note: GC columns do not have a specific directional flow when received from the manufacturer. Upon initial use of your new Zebron column, Phenomenex recommends ™ the practice of dedicating one specific end of the column for injector installation only. This is particularly important when dealing with active/caustic or contaminating compounds. - Page 3 Short-Style Ferrules AG0-8320 Cool-Lock GC Capillary Nut For Use with AG0-8420 Cool-Lock Nut Installation Gauge Long-Style Ferrules **Guaranteed fit for Shimadzu QP-2010 and QP 2014 GC systems AG0-8349 Cool-Lock Nut Installation Gauge Replacement Ferrules AG0-4701 GC Capillary Ferrules Graphite ⁄...
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Page 4: Gc Column Conditioning And Testing
Standards to establish the baseline performance characteristics of your column and to monitor any changes over time. Test solutions should evaluate column inertness, efficiency and resolution in an easy-to-use format. Most Phenomenex test mixes are supplied ready to use at a concentration of 250 μg/mL in glass ampules. -
Page 5: Gc Hints And Tips
The easiest way to reduce column contamination is to add a short, high 3. Use a Wool Liner temperature bake out at the end of the standard GC method. This bake out helps remove high boiling contaminants that would otherwise remain in the column The liner is the first line of defense for the column and the style chosen can make and cause damage. -
Page 6: Carrier Gas Selection And Flow Optimization
Ensure a flow of carrier gas through the column at 100-200 °C. For long-term film columns, nitrogen yields the highest number of theoretical plates. storage, disconnect from the GC and cap or seal the capillary ends. Oxygen However, the optimal velocity is fairly low (long analysis times), and the and moisture can degrade or irreversibly damage the column, especially loss in efficiency at higher velocities is high. -
Page 7: Chemical Compatibility / Solvent Rinsing Of Zebron ™ Capillary Columns
GC Hints and Tips D. Chemical Compatibility / Solvent Rinsing of Zebron Capillary Columns ™ Important! Water and organic solvents such as those listed in Table 1 will not damage Zebron column stationary phases. However, inorganic acids and bases should be completely avoided or rapid degradation and permanent damage to the stationary phase will result. - Page 8 500/Pk AF0-1202-52 500/Pk Please inquire with Phenomenex about other Phenex Syringe filters offerings. Above syringe filters are non-sterile. Housing is made of medical-grade polypropylene (PP) and offer luer lock inlet/slip outlet connections, unless otherwise indicated. If Phenex Syringe Filters do not perform ™...
- Page 9 2/pk AG0-7521 Gold Inlet Base Seal, split (double groove/cross), 0.8 mm 5182-9652 10/pk Replacement Inlet Seal Washers AG0-8397 Inlet Seal Washers, Gold Plated, for Agilent GC injection port — 12/pk GuideRight Injection Hole Septa Long Graphite Ferrules ™ The GuideRight through-hole guides the needle during injection.
- Page 10 HS 100 / 101 / 40 • AH0-7550 Aluminum Seal, with Pharma-Fix molded Tekmar-Dohrmann 7000HT • pressure-release septum • ThermoQuest HS 850, Trace GC AG0-8350 Aluminum Seal, Magnetic Crimp Cap, 20 mm 2000 + HS 2000 TEF/SIL, blue Varian Genesis • • Limited Volume Vials QsertVial ™...
- Page 11 Large Volume 96-Well Plates for High-Throughput 20 x 2.0 mm Selecting a SPE Sorbent: Please call your Phenomenex Sample Preparation Specialist for help in selecting the right SPE sorbent for your analysis. Trademarks Zebron, Inferno, Cool-Lock, Phenex, Strata-X, Guardian, GuideRight, PhenoRed, PhenoGreen, and WAX...
- Page 12 Corporate Office USA 09-4780951 09-4780952 (310) 212-0555 nzinfo@phenomenex.com (310) 328-7768 info@phenomenex.com Puerto Rico (800) 541-HPLC (310) 328-7768 info@phenomenex.com United Kingdom www.phenomenex.com 01625-501367 Phenomenex products are available worldwide. For the distributor in your country, 01625-501796 contact Phenomenex USA, International Department at international@phenomenex.com ukinfo@phenomenex.com...
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