Phenomenex Gemini C18 Care And Use

pH Flexible LC
Gemini HPLC/UHPLC Columns
Tips for Care and Use

General Information

Each Gemini column manufactured by Phenomenex is individually prepared and tested. Every column is supplied with a
Certificate of Quality Assurance (CQA) which indicates testing conditions, operating parameters, and column details. The
column details, including specifications and performance test results should be entered into your information management
system for easy tracking and reference. Electronic copies of your column's quality documentation can also be acquired at:
www.phenomenex.com/mysupport.

Inspection

Upon receipt of column, please verify that the column you received is the one you ordered (i.e. dimensions, particle size, media).
Additionally, please check the column for any physical damage potentially caused during shipment. Test the column immediately to
verify performance and record the result of your test in your column information management system.

Column Characteristics

Gemini
Phases Shipping Solvent
†
C18
Acetonitrile / Water (65:35)
NX-C18 Acetonitrile / Water (75:25)
C6-Phenyl
Acetonitrile / Water (65:35)
† Depending on column dimensions, organic to aqueous solvent ratios may vary slightly.
* pH stability under gradient conditions. pH stability is 1.5-10 under isocratic conditions.

Typical Flow Rate, Backpressure, Temperature:

Here are some typical values for common dimensions of Gemini HPLC and UHPLC columns. These numbers are not absolute values
and can differ based on LC system, running parameters and sample analytes/matrix. The values below have been created using a
solvent system of Acetonitrile and Water.
Particle Internal Typical Flow
Size (µm) Diameter (mm) (mL/min)
3 0.3 0.005
3 0.5 0.015
3 1.0 0.05
3 2.0 0.2
3 3.0 0.5
3 4.6 1
5 2.0 0.2
5 3.0 0.5
5 4.6 1
5 10.0 5
5 21.2 20
5 30.0 40
10 4.6 1
10 10.0 5
10 21.2 20
10 30.0 40
10 50.0 50
** Column Length
Phenomenex products are available worldwide.
www.phenomenex.com/mysupport
Particle Pore Surface
Sizes Size Area
(µm) (Å) (m
3,5,10 110 375
3,5,10 110 375
3,5 110 375
Typical Pressure (PSI)
50 mm** 150 mm** 250 mm**
500 1000 NA
700 1400 NA
800 1000 NA
650 1550 NA
950 1900 2700
1000 1700 2600
400 650 850
550 1100 1550
1400 1500 1950
1400 1600 1800
500 800 1400
500 900 1200
NA NA 1400
NA NA 1400
NA 400 500
NA 400 600
NA 300 300
Carbon
Load Reversed
/g) (%) pH Stability Phase
2
14 1.0-12.0*
14 1.0-12.0*
12 1.0-12.0*
Typical Flow Rates (Independent of particle size):
i. 1.0 mL/min for 4.6 mm ID
ii. 0.2-0.6 mL/min for 2.1 mm ID
Maximum Backpressure:
iii. > 5,000 psi (345 bar) may compromise column longevity.
Maximum Temperature:
iv. Recommended max temperature for Gemini LC columns is
60°C, however temperature limits are dependent on your
running parameters. Running at a pH greater than 8 at 60°C will
compromise column lifetime.
v. Continuous use of Gemini columns at the maximum
temperature limit may compromise column longevity.
100%
Normal Aqueous
Phase HILIC Stable
QUALITY
MANAGEMENT SYSTEM
CERTIFIED BY DNV GL
9001:2008