Transmitted Light Insert; Twin Wavelength Method - LEITZ LABORLUX S Instructions Manual

24
Transmitled light insert
Instead ofa filter b lock, a trans mitted light insert can be used in
the PLO EMOPAK for normal transmitted light microscopy.
The microsco pe can thus be used a lte rna te ly for transmitted
light b rightti e ld wo rk and inc ide nt li g ht n uoresce nce studies,
with out the Leitz PLOEMOPAK having to he removed from
the microscope.
Fig. 29
The arrowed number indicates th e filter system
In
eITect.
The stop (t)
clicks
into
place when the bloc k
is
correctly positioned. Only fi lter
block s I
and
lcan now be
us ed.
Fori ntercha nge between all th ree posi·
tions, pull th e stop upwards and
clamp by
rota ting
sligh tly.
1 x
-
•
TWin wavelength method
This method is used for recognition of small amounts of one
fluoro chrome in th e presence of relatively large quantities of a
seco nd . By successive excitatio n w ith two wavelengths, the
specific nuorescence can be observed se parately from th e
other. Two differe nt components can th en, for example, be
recognised inside one or in separa te cells.
Please re fer to the instructio ns for th e Lamphousing
103 Z
(Order no.
933 534)
for informatio n on insertio n, ope ratio n and
centratioD of the me rcury maximum-press ure lamp.