Phase Contrast Objectives; Phase Contrast With The Ukl Universal Condenser - LEITZ LABORLUX S Instructions Manual

16
Annular
sto p
EF objectives
PLAN objectives
I
10/0.25
PHACO I PLAN
10/0.25 PHACO I
2
25/0.40 PHACO 2 PLAN 20/0.40
PHACO I
40/0.65 PHACO 2 PLAN 40/0.65
PHAC02
3 10011.25 PHACO 3
PLAN 10011.25 OEL PHACO 3
Phase conlr.lsl objectives
Phase contrast with the UKL universal condenser
[nsert the UKL condenser into the condenser mount (1.9) and
bring to its highest position with the vertical adjustm en t (1.8).
Set the aperture diaphragm (9.5) to "10" (gree n). Put a speci­
me n on the stage. Turn in th e 10/0.25 PH ACO I objecti ve.
Set the annu lar stop turret at pos ition I and foc us (he specimen
with the coa rse and fine drive. Cl ose the field diaphragm. Set
Ihe condenser with the verti cal adjustment (1.8) so that the
edges of th e closed fi e ld diaphragm are in sharp foclis. Centre
the
image of the field diaphragm
wi th th e two centdng screws.
Open th e tield diaphragm until it just di sappe ars from the field
of view.
Remove an eyepiece from the eyepiec e tube and insert
the foc usin g telescope. Loosen the clamp ring on th e focusing
telescope and adjust the eyelens until light and phase rings are
in sharp focus. Using the cenlring screws (press in and turn)
adjust the lig ht ring so that it is exactly covered by the phase
ring o f the objective . Ce nlral io n mu st be c<:Irried o ut o nce for
all objective/annular stop combina tion s <:Ind is then retained
for all further settings.
Fig. 17 Phase ring and annular stop as seen through the fo cusing
telescope.
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